LOCUS       pENTRYhRIP4-stop        4644 bp    DNA     circular     15-JUN-2006
DEFINITION  LMBP 5346.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|405002363|
COMMENT     VNTNAME|pENTRYhRIP4-stop|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pENTRYhRIP4-stop" 0 4644 
COMMENT       (CDBMol 0 0 1 1786 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 5 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI" "XhoI" "BclI" "BglI" "BglII" "BstXI" "DpnI" "DraI" 
COMMENT         "EcoRV" "HincII" "KpnI" "PvuI" "PvuII" "SacI" "SalI" "ScaI" "SfiI" 
COMMENT         "TaqI" "XbaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 1 0 0 0 0 1 4644 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 1 4325 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 8 57 0 350 0 592 0 1229 0 
COMMENT            1553 0 1618 0 2598 0 2860 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 485 0 1309 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 497 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1730 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 504 0 527 0 845 0 2818 0 
COMMENT            0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 6 1271 0 1785 0 2202 0 2405 
COMMENT            0 2796 0 2986 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 2600 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 2598 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2860 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "BclI" "tgatca" 2 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 3 1050 0 1453 0 2202 
COMMENT             0 0 "") #13=(CRSite (CStringList) "BglII" "agatct" 2 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 4 721 0 846 0 1702 
COMMENT             0 2131 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "DpnI" "gatc" 3 0 17 55 0 487 0 852 0 1136 0 
COMMENT             1311 0 1404 0 1660 0 1701 0 1778 0 1988 0 3390 0 3520 0 3772 0 3981 
COMMENT             0 3989 0 4000 0 4075 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "DraI" "tttaaa" 4 0 1 462 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2867 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "HincII" "gtyrac" 4 0 5 480 0 679 0 1996 0 
COMMENT             2095 0 2395 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 2 495 0 1710 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 1137 0 3521 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "PvuII" "cagctg" 4 0 2 1519 0 2981 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "SacI" "gagctc" 6 0 2 1563 0 1971 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 478 0 677 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "ScaI" "agtact" 4 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "TaqI" "tcga" 2 0 14 479 0 555 0 678 0 1104 0 
COMMENT             1482 0 1545 0 2376 0 2574 0 2697 0 2861 0 3120 0 3486 0 3889 0 4541 
COMMENT             0 0 "") 
COMMENT        #27=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2869 0 0 "")) 
COMMENT       (CObList 
COMMENT        #28=(CFSignal (CObList) "rrnB T2 transcription terminator" 43 0 1 281 
COMMENT             "") 
COMMENT        #29=(CFSignal (CObList) "rrnB T1 transcription terminator" 43 0 1 106 
COMMENT             "") 
COMMENT        #30=(CFSignal (CObList) "pUC origin" 33 0 0 3968 4641 0 (CStringList) 
COMMENT        #31=(CFSignal (CObList) "Kan(R)" 4 0 0 3095 3904 0 (CStringList) 
COMMENT        #32=(CFSignal (CObList) "attL2" 86 0 1 2873 2972 0 (CStringList) 
COMMENT        #33=(CFSignal (CObList) "attL1" 86 0 0 358 457 0 (CStringList) 
COMMENT        #34=(CFSignal (CObList) "hRIP4-Stop" 4 0 0 505 2856 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #35=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #36=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                    "pENTRYhRIP4-stop" 2) 5 "" 0 4) 
COMMENT              #37=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pENTRYhRIP4-stop'" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "DMB 5346" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: kkersse. Original author: kkersse" 
COMMENT                          1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 03/15/06 12:39" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 06/15/06 02:59" 1) 
COMMENT                     #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4644 bp" 1) 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #46=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 15-JUN-2006" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #49=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 4 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "kkersse" 1))) 
COMMENT                   "Author") 
COMMENT              #51=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #52=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "kkersse" 1))) 
COMMENT                   "Original Author") 
COMMENT              #53=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #54=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #55=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 27164488 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #56=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 4325 " 1))))) "ApaLI: 1 site") 1 5 
COMMENT                          "GTGCAC" "CACGTG") 
COMMENT                     #58=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 27164568 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "AvaI: 8 sites") 1 5 "CYCGRG" 
COMMENT                          "GRGCYC") 
COMMENT                     #59=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 27173768 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #60=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 485 " 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 1309 " 1))))) "BamHI: 2 sites") 1 
COMMENT                          5 "GGATCC" "CCTAGG") 
COMMENT                     #63=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 26881352 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "BclI: 0 sites") 1 5 "TGATCA" 
COMMENT                          "ACTAGT") 
COMMENT                     #64=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 27174648 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #65=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #12# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #12# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 1050 " 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #12# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 1453 " 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #12# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 2202 " 1))))) "BglI: 3 sites") 7 4 
COMMENT                          "GCCNNNNNGGC" "CGGNNNNNCCG") 
COMMENT                     #69=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 27161672 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "BglII: 0 sites") 1 5 "AGATCT" 
COMMENT                          "TCTAGA") 
COMMENT                     #70=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 36318960 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #71=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #14# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #14# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 721 " 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #14# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 846 " 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #14# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 1702 " 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #14# 4) 1 2 2 0 191) 
COMMENT                                        " N4: 2131 " 1))))) "BstXI: 4 sites") 8 
COMMENT                          4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
COMMENT                     #76=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 27165288 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT" 
COMMENT                          "TAGCTA") 
COMMENT                     #77=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 36319360 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "DpnI: 17 sites") 2 2 "GATC" "CTAG") 
COMMENT                     #78=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 36319840 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #79=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #16# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #16# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 462 " 1))))) "DraI: 1 site") 3 3 
COMMENT                          "TTTAAA" "AAATTT") 
COMMENT                     #81=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 27161928 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #82=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 497 " 1))))) "EcoRI: 1 site") 1 5 
COMMENT                          "GAATTC" "CTTAAG") 
COMMENT                     #84=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 36318640 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #85=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #17# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #17# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 2867 " 1))))) "EcoRV: 1 site") 3 3 
COMMENT                          "GATATC" "CTATAG") 
COMMENT                     #87=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 26892920 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #88=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #18# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #18# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 480 " 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #18# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 679 " 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #18# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 1996 " 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #18# 4) 1 2 2 0 191) 
COMMENT                                        " N4: 2095 " 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #18# 5) 1 2 2 0 191) 
COMMENT                                        " N5: 2395 " 1))))) "HincII: 5 sites") 3 
COMMENT                          3 "GTYRAC" "CARYTG") 
COMMENT                     #94=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 21377840 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #95=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 1730 " 1))))) "HindIII: 1 site") 1 
COMMENT                          5 "AAGCTT" "TTCGAA") 
COMMENT                     #97=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 36319760 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #98=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #19# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #19# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 495 " 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (1 #19# 2) 1 2 2 0 191) 
COMMENT                                         " N2: 1710 " 1))))) "KpnI: 2 sites") 5 
COMMENT                          1 "GGTACC" "CCATGG") 
COMMENT                     #101=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 21379520 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
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COMMENT                                    (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #103=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 504 " 1) 
COMMENT                                     #104=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #6# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 527 " 1) 
COMMENT                                     #105=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #6# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 845 " 1) 
COMMENT                                     #106=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #6# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 2818 " 1))))) "NcoI: 4 sites") 
COMMENT                           1 5 "CCATGG" "GGTACC") 
COMMENT                     #107=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 21399040 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList)) "PstI: 6 sites") 5 1 "CTGCAG" 
COMMENT                           "GACGTC") 
COMMENT                     #108=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 36319680 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #109=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #20# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #20# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 1137 " 1) 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #20# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 3521 " 1))))) "PvuI: 2 sites") 
COMMENT                           4 2 "CGATCG" "GCTAGC") 
COMMENT                     #112=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 26889176 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
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COMMENT                                    (CObjectList 
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COMMENT                                           " N1: 1519 " 1) 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #21# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 2981 " 1))))) "PvuII: 2 sites") 
COMMENT                           3 3 "CAGCTG" "GTCGAC") 
COMMENT                     #116=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 26885816 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
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COMMENT                                    (CParagraph 0 (10 #22# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #22# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 1563 " 1) 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #22# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 1971 " 1))))) "SacI: 2 sites") 
COMMENT                           5 1 "GAGCTC" "CTCGAG") 
COMMENT                     #120=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 26899888 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
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COMMENT                                    (CObjectList 
COMMENT                                     #122=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #23# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 478 " 1) 
COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #23# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 677 " 1))))) "SalI: 2 sites") 1 
COMMENT                           5 "GTCGAC" "CAGCTG") 
COMMENT                     #124=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 26275824 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList)) "ScaI: 0 sites") 3 3 "AGTACT" 
COMMENT                           "TCATGA") 
COMMENT                     #125=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 26265416 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList)) "SfiI: 0 sites") 8 5 "GGCCNNNNNGGCC" 
COMMENT                           "CCGGNNNNNCCGG") 
COMMENT                     #126=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 21398000 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
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COMMENT                                    (CObjectList 
COMMENT                                     #128=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #8# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 2600 " 1))))) "SmaI: 1 site") 3 
COMMENT                           3 "CCCGGG" "GGGCCC") 
COMMENT                     #129=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 27017232 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList)) "TaqI: 14 sites") 1 3 "TCGA" "AGCT") 
COMMENT                     #130=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 26886296 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
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COMMENT                                    (CObjectList 
COMMENT                                     #132=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #27# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 2869 " 1))))) "XbaI: 1 site") 1 
COMMENT                           5 "TCTAGA" "AGATCT") 
COMMENT                     #133=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 27161592 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #134=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #10# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #135=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #10# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 2860 " 1))))) "XhoI: 1 site") 1 
COMMENT                           5 "CTCGAG" "GAGCTC") 
COMMENT                     #136=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 27163208 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #137=(CGroupPar 
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COMMENT                                   (LOGFONT 51 19 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Hinc}II (2395)" "@N (@S)" 5 -4.89555 
COMMENT                                   -15.681 2.48303 14.6425 5.06822 0.953798 
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COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #322=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -531 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 1953066364 
COMMENT       (CStringList) 1869112175 1769104494 (CObList)))
FEATURES             Location/Qualifiers
     terminator      complement(281..308)
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      complement(106..149)
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     rep_origin      3968..4641
                     /vntifkey="33"
                     /label=pUC\origin
     CDS             3095..3904
                     /vntifkey="4"
                     /label=Kan(R)
     misc_recomb     complement(2873..2972)
                     /vntifkey="86"
                     /label=attL2
     misc_recomb     358..457
                     /vntifkey="86"
                     /label=attL1
     CDS             <505..2856
                     /vntifkey="4"
                     /label=hRIP4-Stop
BASE COUNT     1057 a      1253 c      1315 g      1019 t 
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg 
      481 actggatccg gtaccgaatt caccatggag ggcgacggcg ggaccccatg ggccctggcg 
      541 ctgctgcgca ccttcgacgc gggcgagttc acgggctggg agaaggtggg ctcgggcggc 
      601 ttcgggcagg tgtacaaggt gcgccatgtc cactggaaga cctggctggc catcaagtgc 
      661 tcgcccagcc tgcacgtcga cgacagggag cgcatggagc ttttggaaga agccaagaag 
      721 atggagatgg ccaagtttcg ctacatcctg cctgtgtatg gcatctgccg cgaacctgtc 
      781 ggcctggtca tggagtacat ggagacgggc tccctggaaa agctgctggc ttcggagcca 
      841 ttgccatggg atctccggtt ccgaatcatc cacgagacgg cggtgggcat gaacttcctg 
      901 cactgcatgg ccccgccact cctgcacctg gacctcaagc ccgcgaacat cctgctggat 
      961 gcccactacc acgtcaagat ttctgatttt ggtctggcca agtgcaacgg gctgtcccac 
     1021 tcgcatgacc tcagcatgga tggcctgttt ggcacaatcg cctacctccc tccagagcgc 
     1081 atcagggaga agagccggct cttcgacacc aagcacgatg tatacagctt tgcgatcgtc 
     1141 atctggggcg tgctcacaca gaagaagccg tttgcagatg agaagaacat cctgcacatc 
     1201 atggtgaagg tggtgaaggg ccaccgcccc gagctgccgc ccgtgtgcag agcccggccg 
     1261 cgcgcctgca gccacctgat acgcctcatg cagcggtgct ggcaggggga tccgcgagtt 
     1321 aggcccacct tccaagaaat tacttctgaa accgaggacc tgtgtgaaaa gcctgatgac 
     1381 gaagtgaaag aaactgctca tgatctggac gtgaaaagcc ccccggagcc caggagcgag 
     1441 gtggtgcctg cgaggctcaa gcgggcctct gcccccacct tcgataacga ctacagcctc 
     1501 tccgagcttc tctcacagct ggactctgga gtttcccagg ctgtcgaggg ccccgaggag 
     1561 ctcagccgca gctcctctga gtccaagctg ccatcgtccg gcagtgggaa gaggctctcg 
     1621 ggggtgtcct cggtggactc cgccttctct tccagaggat cactgtcgct gtcctttgag 
     1681 cgggaacctt caaccagcga tctgggtacc acagacgtcc agaagaagaa gcttgtggat 
     1741 gccatcgtgt ccggggacac cagcaaactg atgaagatcc tgcagccgca ggacgtggac 
     1801 ctggcactgg acagcggtgc cagcctgctg cacctggcgg tggaggccgg gcaagaggag 
     1861 tgcgccaagt ggctgctgct caacaatgcc aaccccaacc tgagcaaccg taggggctcc 
     1921 accccgttgc acatggccgt ggagaggagg gtgcggggtg tcgtggagct cctgctggca 
     1981 cggaagatca gtgtcaacgc caaggatgag gaccagtgga cagccctcca ctttgcagcc 
     2041 cagaacgggg atgagtctag cacacggctg ctgttggaga agaacgcctc ggtcaacgag 
     2101 gtggactttg agggccggac gcccatgcac gtggcctgcc agcacgggca ggagaatatc 
     2161 gtgcgcatcc tgctgcgccg aggcgtggac gtgagcctgc agggcaagga tgcctggctg 
     2221 ccactgcact acgctgcctg gcagggccac ctgcccatcg tcaagctgct ggccaagcag 
     2281 ccgggggtga gtgtgaacgc ccagacgctg gatgggagga cgccattgca cctggccgca 
     2341 cagcgcgggc actaccgcgt ggcccgcatc ctcatcgacc tgtgctccga cgtcaacgtc 
     2401 tgcagcctgc tggcacagac acccctgcac gtggccgcgg agacggggca cacgagcact 
     2461 gccaggctgc tcctgcatcg gggcgctggc aaggaggccg tgacctcaga cggctacacc 
     2521 gctctgcacc tggctgcccg caacggacac ctggccactg tcaagctgct tgtcgaggag 
     2581 aaggccgatg tgctggcccg gggacccctg aaccagacgg cgctgcacct ggctgccgcc 
     2641 cacgggcact cggaggtggt ggaggagttg gtcagcgccg atgtcattga cctgttcgac 
     2701 gagcaggggc tcagcgcgct gcacctggcc gcccagggcc ggcacgcaca gacggtggag 
     2761 actctgctca ggcatggggc ccacatcaac ctgcagagcc tcaagttcca gggcggccat 
     2821 ggccccgccg ccacactcct gcggcgaagc aagacccact cgagatatct agacccagct 
     2881 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag 
     2941 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgcagctct ggcccgtgtc 
     3001 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact 
     3061 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc 
     3121 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 
     3181 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga 
     3241 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 
     3301 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 
     3361 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga 
     3421 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 
     3481 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca 
     3541 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 
     3601 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga 
     3661 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 
     3721 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 
     3781 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 
     3841 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt 
     3901 ctaatcagaa ttggttaatt ggttgtaaca ttattcagat tgggccccgt tccactgagc 
     3961 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 
     4021 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 
     4081 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 
     4141 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 
     4201 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 
     4261 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 
     4321 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 
     4381 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 
     4441 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 
     4501 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 
     4561 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 
     4621 ttgctggcct tttgctcaca tgtt 
//
