LOCUS       pENTRYhRIP4+stop        4645 bp    DNA     circular     17-MAY-2006
DEFINITION  LMBP 5347.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|410708889|
COMMENT     VNTNAME|pENTRYhRIP4+stop|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI" "XhoI" "PvuII") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 1 1467 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 8 2 0 1843 0 2136 0 2378 0 
COMMENT            3015 0 3339 0 3404 0 4384 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 2271 0 3095 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2283 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 3516 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 2290 0 2313 0 2631 0 4604 
COMMENT            0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 6 128 0 3057 0 3571 0 3988 
COMMENT            0 4191 0 4582 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 4386 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 4384 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "PvuII" "cagctg" 4 0 2 123 0 3305 0 0 "")) 
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COMMENT             1 "") 
COMMENT        #13=(CFSignal (CObList) "rrnB T1 transcription terminator" 43 0 1 1892 
COMMENT             1 "") 
COMMENT        #14=(CFSignal (CObList) "pUC origin" 33 0 0 1110 1783 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "attL2" 86 0 1 15 114 0 (CStringList) 
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COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: kkersse. Original author: kkersse" 
COMMENT                          1) 
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COMMENT                          "Created: 05/17/06 01:48" 1) 
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COMMENT                          "Last Modified: 05/17/06 02:13" 1) 
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COMMENT                          "length: 4645 bp" 1) 
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COMMENT                          "CYCGRG" "GRGCYC") 
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COMMENT                          "CCATGG" "GGTACC") 
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COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
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FEATURES             Location/Qualifiers
     terminator      complement(2067..2094)
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      complement(1892..1935)
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     rep_origin      1110..1783
                     /vntifkey="33"
                     /label=pUC\origin
     CDS             237..1046
                     /vntifkey="4"
                     /label=Kan(R)
     misc_recomb     complement(15..114)
                     /vntifkey="86"
                     /label=attL2
     misc_recomb     2144..2243
                     /vntifkey="86"
                     /label=attL1
     CDS             <2291..4645
                     /vntifkey="4"
                     /label=hRIP4+stop
BASE COUNT     1057 a      1254 c      1317 g      1017 t 
ORIGIN
        1 ctcgagatat ctagacccag ctttcttgta caaagttggc attataagaa agcattgctt 
       61 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc 
      121 agctgcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata 
      181 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga 
      241 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat 
      301 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt 
      361 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg 
      421 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca 
      481 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa 
      541 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc 
      601 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg 
      661 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga 
      721 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata 
      781 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc 
      841 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag 
      901 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac 
      961 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc 
     1021 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cattattcag 
     1081 attgggcccc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 
     1141 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 
     1201 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 
     1261 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 
     1321 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 
     1381 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 
     1441 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 
     1501 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 
     1561 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 
     1621 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 
     1681 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 
     1741 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 
     1801 cccctgattc tgtggataac cgtattaccg ctagcatgga tctcggggac gtctaactac 
     1861 taagcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cggaagactg 
     1921 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc 
     1981 gggagcggat ttgaacgttg tgaagcaacg gcccggaggg tggcgggcag gacgcccgcc 
     2041 ataaactgcc aggcatcaaa ctaagcagaa ggccatcctg acggatggcc tttttgcgtt 
     2101 tctacaaact cttcctgtta gttagttact taagctcggg ccccaaataa tgattttatt 
     2161 ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttt ttataatgcc 
     2221 aactttgtac aaaaaagcag gctctttaaa ggaaccaatt cagtcgactg gatccggtac 
     2281 cgaattcacc atggagggcg acggcgggac cccatgggcc ctggcgctgc tgcgcacctt 
     2341 cgacgcgggc gagttcacgg gctgggagaa ggtgggctcg ggcggcttcg ggcaggtgta 
     2401 caaggtacgc catgtccact ggaagacctg gctggccatc aagtgctcgc ccagcctgca 
     2461 cgtcgacgac agggagcgca tggagctttt ggaagaagcc aagaagatgg agatggccaa 
     2521 gtttcgctac atcctgcctg tgtatggcat ctgccgcgaa cctgtcggcc tggtcatgga 
     2581 gtacatggag acgggctccc tggaaaagct gctggcttcg gagccattgc catgggatct 
     2641 ccggttccga atcatccacg agacggcggt gggcatgaac ttcctgcact gcatggcccc 
     2701 gccactcctg cacctggacc tcaagcccgc gaacatcctg ctggatgccc actaccacgt 
     2761 caagatttct gattttggtc tggccaagtg caacgggctg tcccactcgc atgacctcag 
     2821 catggatggc ctgtttggca caatcgccta cctccctcca gagcgcatca gggagaagag 
     2881 ccggctcttc gacaccaagc acgatgtata cagctttgcg atcgtcatct ggggcgtgct 
     2941 cacacagaag aagccgtttg cagatgagaa gaacatcctg cacatcatgg tgaaggtggt 
     3001 gaagggccac cgccccgagc tgccgcccgt gtgcagagcc cggccgcgcg cctgcagcca 
     3061 cctgatacgc ctcatgcagc ggtgctggca gggggatccg cgagttaggc ccaccttcca 
     3121 agaaattact tctgaaaccg aggacctgtg tgaaaagcct gatgacgaag tgaaagaaac 
     3181 tgctcatgat ctggacgtga aaagcccccc ggagcccagg agcgaggtgg tgcctgcgag 
     3241 gctcaagcgg gcctctgccc ccaccttcga taacgactac agcctctccg agctgctctc 
     3301 acagctggac tctggagttt cccaggctgt cgagggcccc gaggagctca gccgcagctc 
     3361 ctctgagtcc aagctgccat cgtccggcag tgggaagagg ctctcggggg tgtcctcggt 
     3421 ggactccgcc ttctcttcca gaggatcact gtcgctgtcc tttgagcggg aaccttcaac 
     3481 cagcgatctg ggcaccacag acgtccagaa gaagaagctt gtggatgcca tcgtgtccgg 
     3541 ggacaccagc aaactgatga agatcctgca gccgcaggac gtggacctgg cactggacag 
     3601 cggtgccagc ctgctgcacc tggcggtgga ggccgggcaa gaggagtgcg ccaagtggct 
     3661 gctgctcaac aatgccaacc ccaacctgag caaccgtagg ggctccaccc cgttgcacat 
     3721 ggccgtggag aggagggtgc ggggtgtcgt ggagctcctg ctggcgcgga agatcagtgt 
     3781 caacgccaag gatgaggacc agtggacagc cctccacttt gcagcccaga acggggacga 
     3841 gtctagcaca cggctgctgt tggagaagaa cgcctcggtc aacgaggtgg actttgaggg 
     3901 ccggacgccc atgcacgtgg cctgccagca cgggcaggag aatatcgtgc gcatcctgct 
     3961 gcgccgaggc gtggacgtga gcctgcaggg caaggatgcc tggctgccac tgcactacgc 
     4021 tgcctggcag ggccacctgc ccatcgtcaa gctgctggcc aagcagccgg gggtgagtgt 
     4081 gaacgcccag acgctggatg ggaggacgcc attgcacctg gccgcacagc gcgggcacta 
     4141 ccgcgtggcc cgcatcctca tcgacctgtg ctccgacgtc aacgtctgca gcctgctggc 
     4201 acagacaccc ctgcacgtgg ccgcggagac ggggcacacg agcactgcca ggctgctcct 
     4261 gcatcggggc gctggcaagg aggccgtgac ctcagacggc tacaccgctc tgcacctggc 
     4321 tgcccgcaac ggacacctgg ccactgtcaa gctgcttgtc gaggagaagg ccgatgtgct 
     4381 ggcccgggga cccctgaacc agacggcgct gcacctggct gccgcccacg ggcactcgga 
     4441 ggtggtggag gagttggtca gcgccgatgt cattgacctg ttcgacgagc aggggctcag 
     4501 cgcgctgcac ctggccgccc agggccggca cgcacagacg gtggagactc tgctcaggca 
     4561 tggggcccac atcaacctgc agagcctcaa gttccagggc ggccatggcc ccgccgccac 
     4621 actcctgcgg cgaagcaaga cctag 
//
