LOCUS       pCAGGS-E-mABIN-1(469-524)        4977 bp    DNA     circular     24-OCT-2007
DEFINITION  LMBP 5376.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5376
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
            
COMMENT     VNTDATE|457355639|
COMMENT     VNTNAME|pCAGGS-E-mABIN-1(469-524)|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
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COMMENT       (CObList #0=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 192 0 728 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 2698 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 733 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4 0 0 "")) 
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COMMENT            (CStringList) 1 1 1 1 "16..54") 
COMMENT        #6=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 359 715 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1123 1197 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1123 1197 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
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COMMENT        #10=(CFSignal (CObList) "\337-globin polyA" 43 0 0 267 358 1 
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COMMENT        #16=(CFSignal (CObList) "ampicillin" 4 0 1 2254 3114 1 (CStringList) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 09/13/07 11:13" 1) 
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COMMENT                                        "Mouse A20-binding inhibitor of NF-kappaB activation 1 cDNA; aa 469-524" 
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FEATURES             Location/Qualifiers
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     misc_feature    359..715
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit  ß-globin gene"
     terminator      complement(1123..1197)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      1123..1197
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      complement(1312..1713)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      267..358
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     promoter        complement(793..875)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      1017..1092
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     intron          4002..4926
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="intron of the chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3633..4974
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3250..3627
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     CDS             complement(2254..3114)
                     /vntifkey="4"
                     /label=ampicillin
     CDS             64..234
                     /vntifkey="4"
                     /label=mABIN-1(469-524)
                     /note="Mouse A20-binding inhibitor of NF-kappaB activation 1 cDNA; aa 469-524"
BASE COUNT     1061 a      1317 c      1388 g      1211 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcagtgacac agaatgagtt gctgaaacag caggtaaaga tctttgaaga ggacttccag 
      121 agggaacgga gtgaccgtga acgcatgaat gaagagaagg aggagctgaa gaagcaagta 
      181 gagaagctgc aggcccaggt caccctgact aatgcccagc tcaaaactct ctgaccccca 
      241 atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca 
      301 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa 
      361 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt 
      421 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg 
      481 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc 
      541 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg 
      601 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt 
      661 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg 
      721 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 
      781 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctgggtgcct 
      841 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 
      901 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac 
      961 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 
     1021 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 
     1081 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct 
     1141 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 
     1201 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc 
     1261 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 
     1321 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
     1381 actcaaaggc ggtaatacgg ttatccacag aatcagggat aacgcaggaa agaacatgtg 
     1441 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
     1501 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
     1561 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
     1621 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
     1681 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
     1741 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
     1801 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
     1861 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
     1921 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
     1981 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
     2041 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     2101 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     2161 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     2221 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     2281 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     2341 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     2401 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     2461 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     2521 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
     2581 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     2641 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     2701 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     2761 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     2821 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
     2881 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     2941 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     3001 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     3061 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     3121 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     3181 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     3241 acctgggtcg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 
     3301 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 
     3361 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 
     3421 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg 
     3481 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 
     3541 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 
     3601 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc 
     3661 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt 
     3721 gtgcagcgat gggggcgggg gggggggggg ggcccccccc aggcggggcg gggcggggcg 
     3781 aggggcgggg cggggcgagg cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc 
     3841 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc 
     3901 ggcgggcggg agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc 
     3961 gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc 
     4021 cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc 
     4081 tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg 
     4141 gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct 
     4201 gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg 
     4261 cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc 
     4321 ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc 
     4381 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta 
     4441 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg 
     4501 gcggggcggg gccgcctcgg gccggggagg gctcggggga aggggcgcgg cggcccccgg 
     4561 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc 
     4621 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc 
     4681 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg 
     4741 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg 
     4801 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc 
     4861 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc 
     4921 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaa 
//
