LOCUS       pRH020                  6751 bp    DNA     circular     20-MAY-2014
DEFINITION  LMBP 5431.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5431
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 20-MAY-2014
            
COMMENT     VNTDATE|436794302|
COMMENT     VNTNAME|pRH020|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pRH020" 0 6751 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BglII" "Bsp119I" "BstXI" "Eco32I" "HindIII" "NcoI" "PdiI" 
COMMENT         "PdmI" "ScaI" "Bsp1407I" "Eam1105I" "EcoRV") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6751 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #3=(CRSite (CStringList) "BglII" "agatct" 2 0 1 1944 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "Bsp119I" "ttcgaa" 3 0 1 3187 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 2 2485 0 3650 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "Eco32I" "gatatc" 4 0 1 3596 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1032 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 1798 0 6165 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "PdiI" "gccggc" 4 0 2 402 0 537 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "PdmI" "gaannnnttc" 6 0 2 3183 0 6547 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "ScaI" "agtact" 4 0 3 1914 0 3363 0 6281 0 0 
COMMENT             "") 
COMMENT        #12=(CRSite (CStringList) "Bsp1407I" "tgtaca" 2 0 3 1058 0 2341 0 2743 0 
COMMENT             0 "") 
COMMENT        #13=(CRSite (CStringList) "Eam1105I" "gacnnnnngtc" 7 0 1 4945 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 3596 0 0 "")) 
COMMENT       (CObList 
COMMENT        #15=(CFSignal (CObList) "ccdB" 4 0 0 2284 2589 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2284..2589") 
COMMENT        #16=(CFSignal (CObList) "chloramphenicol" 4 0 0 1283 1942 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1283..1942") 
COMMENT        #17=(CFSignal (CObList) "attR2" 0 0 0 2630 2754 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2630..2754") 
COMMENT        #18=(CFSignal (CObList) "attR1" 0 0 1 1050 1174 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1050..1174)") 
COMMENT        #19=(CFSignal (CObList) "chloramphenicol" 4 0 0 5650 6309 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5650..6309") 
COMMENT        #20=(CFSignal (CObList) "pMB1 ori" 33 0 1 3928 4330 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3928..4330)") 
COMMENT        #21=(CFSignal (CObList) "T7 promoter" 30 0 0 968 984 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "968..984") 
COMMENT        #22=(CFSignal (CObList) "T3 promoter" 30 0 1 3673 3689 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3673..3689)") 
COMMENT        #23=(CFSignal (CObList) "lac promoter" 30 0 1 3753 3836 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3753..3836)") 
COMMENT        #24=(CFSignal (CObList) "RK2/RP4 oriT" 33 0 0 536 646 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "536..646") 
COMMENT        #25=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 1182 1234 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #26=(CFSignal (CObList) "GST" 4 0 0 2789 3442 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2789..3463")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #27=(CFSignal (CObList) "LMBP 5431" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #28=(CFSignal (CObList) "Last modified: 20-MAY-2014" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                   4) 
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COMMENT                    (CObjectList 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pRH020'" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5431." 
COMMENT                          1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 01/31/07 11:45" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 05/20/14 11:46" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6751 bp" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 23-OCT-2007" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #43=(CFolderPar 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "LMBP 5431") 
COMMENT                     #50=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "Last modified: 20-MAY-2014"))) 
COMMENT                   "Annotations") 
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COMMENT                                   #56=(CLinePar 
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COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2630..2754" 1))) "attR2"))) 
COMMENT                          "Attenuator (2 total)") 
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COMMENT                                   #65=(CLinePar 
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COMMENT                                   #66=(CLinePar 
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COMMENT                                   #69=(CLinePar 
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COMMENT                                   #70=(CLinePar 
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COMMENT                                        "Start: 5650  End: 6309" 1) 
COMMENT                                   #77=(CLinePar 
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COMMENT                                   #78=(CLinePar 
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COMMENT                                        "   5650..6309" 1))) "chloramphenicol"))) 
COMMENT                          "CDS (4 total)") 
COMMENT                     #79=(CFolderPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        "Start: 968   End: 984 " 1) 
COMMENT                                   #82=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #83=(CLinePar 
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COMMENT                                 "lacUV5 promoter") 
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COMMENT                                   #88=(CLinePar 
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COMMENT                                        "   complement(3673..3689)" 1))) 
COMMENT                                 "T3 promoter") 
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COMMENT                                        "Start: 3753  End: 3836 (Complementary)" 
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COMMENT                                   #92=(CLinePar 
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COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3753..3836)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (4 total)") 
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COMMENT                                        "Start: 536   End: 646 " 1) 
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COMMENT                                   #98=(CLinePar 
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COMMENT                                        "   536..646" 1))) "RK2/RP4 oriT") 
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COMMENT                                         "   complement(3928..4330)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                                            " N1: 1944 " 1))))) "BglII: 1 site") 
COMMENT                            1 5 "AGATCT" "TCTAGA") 
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COMMENT                             "Bsp119I: 1 site") 2 4 "TTCGAA" "AAGCTT") 
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COMMENT                                            " N3: 2743 " 1))))) 
COMMENT                             "Bsp1407I: 3 sites") 1 5 "TGTACA" "ACATGT") 
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COMMENT                                            " N2: 3650 " 1))))) "BstXI: 2 sites") 
COMMENT                            8 4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
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FEATURES             Location/Qualifiers
     CDS             2284..2589
                     /vntifkey="4"
                     /label=ccdB
     CDS             1283..1942
                     /vntifkey="4"
                     /label=chloramphenicol
     attenuator      2630..2754
                     /vntifkey="0"
                     /label=attR2
     attenuator      complement(1050..1174)
                     /vntifkey="0"
                     /label=attR1
     CDS             2789..3442
                     /vntifkey="4"
                     /label=GST
     CDS             5650..6309
                     /vntifkey="4"
                     /label=chloramphenicol
     rep_origin      complement(3928..4330)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        968..984
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(3673..3689)
                     /vntifkey="30"
                     /label=T3\promoter
     promoter        complement(3753..3836)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      536..646
                     /vntifkey="33"
                     /label=RK2/RP4\oriT
     promoter        1182..1234
                     /vntifkey="30"
                     /label=lacUV5\promoter
BASE COUNT     1737 a      1576 c      1675 g      1763 t 
ORIGIN
        1 ggggatcggt cttgccttgc tcgtcggtga tgtacttcac cagctccgcg aagtcgctct 
       61 tcttgatgga gcgcatgggg acgtgcttgg caatcacgcg caccccccgg ccgttttagc 
      121 ggctaaaaaa gtcatggctc tgccctcggg cggaccacgc ccatcatgac cttgccaagc 
      181 tcgtcctgct tctcttcgat cttcgccagc agggcgagga tcgtggcatc accgaaccgc 
      241 gccgtgcgcg ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg 
      301 ccattgatgc gggccagctc gcggacgtgc tcatagtcca cgacgcccgt gattttgtag 
      361 ccctggccga cggccagcag gtaggccgac aggctcatgc cggccgccgc cgccttttcc 
      421 tcaatcgctc ttcgttcgtc tgaaagcagt acaccttgat aggtgggctg cctttcctgg 
      481 gtggcttggt ttaatcagcc atccgctggc cttcatccgg tacgccgccg gaggccggcc 
      541 agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga 
      601 aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga 
      661 tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg 
      721 atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaaga 
      781 tccgtcgaat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 
      841 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 
      901 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 
      961 gtgattgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga 
     1021 cggtatcgat aagcttatcg atcagatcaa caagtttgta caaaaaagct gaacgagaaa 
     1081 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat 
     1141 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt 
     1201 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cggcgagatt 
     1261 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat 
     1321 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta 
     1381 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca 
     1441 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt 
     1501 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac 
     1561 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt 
     1621 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta 
     1681 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt 
     1741 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat 
     1801 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca 
     1861 tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga 
     1921 tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta 
     1981 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag 
     2041 tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc 
     2101 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc 
     2161 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg 
     2221 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt 
     2281 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat 
     2341 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca 
     2401 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa 
     2461 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa 
     2521 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg 
     2581 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg 
     2641 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat 
     2701 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgatct 
     2761 ggggtcgacg gatccccggg taattaatat gtcccctata ctaggttatt ggaaaattaa 
     2821 gggccttgtg caacccactc gacttctttt ggaatatctt gaagaaaaat atgaagagca 
     2881 tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa aagtttgaat tgggtttgga 
     2941 gtttcccaat cttccttatt atattgatgg tgatgttaaa ttaacacagt ctatggccat 
     3001 catacgttat atagctgaca agcacaacat gttgggtggt tgtccaaaag agcgtgcaga 
     3061 gatttcaatg cttgaaggag cggttttgga tattagatac ggtgtttcga gaattgcata 
     3121 tagtaaagac tttgaaactc tcaaagttga ttttcttagc aagctacctg aaatgctgaa 
     3181 aatgttcgaa gatcgtttat gtcataaaac atatttaaat ggtgatcatg taacccatcc 
     3241 tgacttcatg ttgtatgacg ctcttgatgt tgttttatac atggacccaa tgtgcctgga 
     3301 tgcgttccca aaattagttt gttttaaaaa acgtattgaa gctatcccac aaattgataa 
     3361 gtacttgaaa tccagcaagt atatagcatg gcctttgcag ggctggcaag ccacgtttgg 
     3421 tggtggcgac catcctccaa aatcggatct ggttccgcgt tgaggcgcgc cactcgaggg 
     3481 ggggcccggt accaagatgg cctttggtgg gttgaagaag gaaaaagaca gaaacgactt 
     3541 aattacctac ttgaaaaaag cctgtgagta aacaggcccc ttttcctttg tcgatatcga 
     3601 attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg tggagctcca 
     3661 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt 
     3721 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 
     3781 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
     3841 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 
     3901 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 
     3961 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
     4021 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     4081 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     4141 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     4201 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     4261 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
     4321 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     4381 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     4441 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     4501 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 
     4561 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     4621 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     4681 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     4741 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     4801 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
     4861 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
     4921 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
     4981 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
     5041 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
     5101 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
     5161 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
     5221 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
     5281 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
     5341 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
     5401 gatgcttttc tgtgactggt gagtggggat cctctagagt ccgaataaat acctgtgacg 
     5461 gaagatcact tcgcagaata aataaatcct ggtgtccctg ttgataccgg gaagccctgg 
     5521 gccaactttt ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat 
     5581 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag 
     5641 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat 
     5701 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt 
     5761 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg 
     5821 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg 
     5881 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag 
     5941 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta 
     6001 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg 
     6061 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat 
     6121 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat 
     6181 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat 
     6241 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc 
     6301 ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa 
     6361 taagtgataa taagcggatg aatggcagaa attcggacct gcaggcatgc aactctagag 
     6421 gatccccact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 
     6481 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 
     6541 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 
     6601 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 
     6661 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 
     6721 tgttgaatac tcatactctt cctttttcaa t 
//
