LOCUS       pMSCV-F-BirA            7370 bp    DNA     circular     12-FEB-2009
DEFINITION  LMBP 5689.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5689
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 12-FEB-2009
            
COMMENT     VNTDATE|501519450|
COMMENT     VNTNAME|pMSCV-F-BirA|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pMSCV-F-BirA" 0 7370 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AatII" "Alw44I" "Bst98I" "KpnI" "NcoI" "PvuII" "SpeI" 
COMMENT         "SphI" "SspI" "Tth111I" "XbaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 1 7370 1 7370 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AatII" "gacgtc" 6 0 4 747 0 3080 0 3578 0 6572 
COMMENT            0 0 "") 
COMMENT        #1=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 3 5071 0 6317 0 6814 0 0 
COMMENT            "") 
COMMENT        #2=(CRSite (CStringList) "Bst98I" "cttaag" 2 0 4 35 0 1008 0 1429 0 3767 
COMMENT            0 0 "") 
COMMENT        #3=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 3 407 0 1471 0 4139 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 1439 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "PvuII" "cagctg" 4 0 4 1704 0 4581 0 6945 0 
COMMENT            7301 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "SpeI" "actagt" 2 0 2 662 0 2759 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "SphI" "gcatgc" 6 0 2 2553 0 7045 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SspI" "aatatt" 4 0 3 2333 0 4300 0 6454 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "Tth111I" "gacnnngtc" 5 0 5 392 0 813 0 1219 0 
COMMENT            3076 0 4124 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "XbaI" "tctaga" 2 0 3 220 0 2485 0 3952 0 0 "")) 
COMMENT       (CObList 
COMMENT        #11=(CFSignal (CObList) "psi+ extended packaging signal" 21 0 0 516 1404 
COMMENT             0 (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #12=(CFSignal (CObList) "birA" 4 0 0 1479 2444 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #13=(CFSignal (CObList) "FLAG epitope" 22 0 0 1443 1466 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "mPGK1 promoter" 29 0 0 2506 3013 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "puromycin resistance" 4 0 0 3033 3632 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "ampicillin resistance" 4 0 1 5576 6436 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "pMB1 ori" 33 0 1 4633 5035 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "lac promoter" 30 0 1 4458 4541 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "5' MSCV-PCMV LTR" 29 0 0 1 513 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1..515") 
COMMENT        #20=(CFSignal (CObList) "3' MSCV-PCMV LTR" 43 0 0 3733 4245 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3762..4245")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #21=(CFSignal (CObList) "LMBP 5689" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #22=(CFSignal (CObList) "Last modified: 12-FEB-2009" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #23=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #24=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pMSCV-F-BirA" 2) 
COMMENT                   5 "" 0 4) 
COMMENT              #25=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pMSCV-F-BirA'" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5689" 
COMMENT                          1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 12/12/08 02:57" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 02/12/09 02:55" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7370 bp" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                    (CObjectList 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 12-FEB-2009" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #37=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
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COMMENT                    (CObjectList 
COMMENT                     #39=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #40=(CLinePar (CParagraph 0 (15 #21# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 5689") 
COMMENT                     #41=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #42=(CLinePar (CParagraph 0 (15 #22# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 12-FEB-2009"))) 
COMMENT                   "Annotations") 
COMMENT              #43=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #44=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #45=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1479  End: 2444" 1))) "birA") 
COMMENT                            #47=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3033 (3 #15# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3033  End: 3632" 1))) 
COMMENT                                 "puromycin resistance") 
COMMENT                            #49=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5576 (3 #16# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5576  End: 6436 (Complementary)" 
COMMENT                                        1))) "ampicillin resistance"))) 
COMMENT                          "CDS (3 total)") 
COMMENT                     #51=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #52=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 516   End: 1404" 1))) 
COMMENT                                 "psi+ extended packaging signal"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #54=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #55=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1443  End: 1466" 1))) 
COMMENT                                 "FLAG epitope"))) "Misc. Marker (1 total)") 
COMMENT                     #57=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
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COMMENT                                        "   1..515" 1))) "5' MSCV-PCMV LTR") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2506  End: 3013" 1))) 
COMMENT                                 "mPGK1 promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #64=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #65=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4458  End: 4541 (Complementary)" 
COMMENT                                        1))) "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #67=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #68=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4633  End: 5035 (Complementary)" 
COMMENT                                        1))) "pMB1 ori"))) 
COMMENT                          "Replication Origin (1 total)") 
COMMENT                     #70=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 1 178) 
COMMENT                           (CObjectList 
COMMENT                            #71=(CFolderPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        "Start: 3733  End: 4245" 1) 
COMMENT                                   #73=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3762..4245" 1))) "3' MSCV-PCMV LTR"))) 
COMMENT                          "Terminator (1 total)"))) "Feature Map") 
COMMENT              #75=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #76=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #77=(CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 1439 " 1))))) "NcoI: 1 site") 1 5 
COMMENT                          "CCATGG" "GGTACC") 
COMMENT                     #79=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #80=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 662 " 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #6# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 2759 " 1))))) "SpeI: 2 sites") 1 5 
COMMENT                          "ACTAGT" "TGATCA") 
COMMENT                     #83=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #84=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #7# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 2553 " 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #7# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 7045 " 1))))) "SphI: 2 sites") 5 1 
COMMENT                          "GCATGC" "CGTACG") 
COMMENT                     #87=(CRSFolderPar 
COMMENT                          (CFolderPar 
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COMMENT                            (CObjectList 
COMMENT                             #88=(CGroupPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        " N1: 5071 " 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 6317 " 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #1# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 6814 " 1))))) "Alw44I: 3 sites") 1 
COMMENT                          5 "GTGCAC" "CACGTG") 
COMMENT                     #92=(CRSFolderPar 
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COMMENT                                        " N1: 407 " 1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 1471 " 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 4139 " 1))))) "KpnI: 3 sites") 5 1 
COMMENT                          "GGTACC" "CCATGG") 
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COMMENT                                  (CObjectList 
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COMMENT                                         " N2: 4300 " 1) 
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COMMENT                                         " N3: 6454 " 1))))) "SspI: 3 sites") 3 
COMMENT                          3 "AATATT" "TTATAA") 
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COMMENT                                           " N2: 2485 " 1) 
COMMENT                                     #106=(CLinePar 
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COMMENT                                           " N3: 3952 " 1))))) "XbaI: 3 sites") 
COMMENT                           1 5 "TCTAGA" "AGATCT") 
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COMMENT                                           " N1: 747 " 1) 
COMMENT                                     #110=(CLinePar 
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COMMENT                                           " N2: 3080 " 1) 
COMMENT                                     #111=(CLinePar 
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COMMENT                                           " N3: 3578 " 1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #0# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 6572 " 1))))) "AatII: 4 sites") 
COMMENT                           5 1 "GACGTC" "CTGCAG") 
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COMMENT                                     #118=(CLinePar 
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COMMENT                                           " N4: 3767 " 1))))) "Bst98I: 4 sites") 
COMMENT                           1 5 "CTTAAG" "GAATTC") 
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COMMENT                                           " N3: 6945 " 1) 
COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #5# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 7301 " 1))))) "PvuII: 4 sites") 
COMMENT                           3 3 "CAGCTG" "GTCGAC") 
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COMMENT                                           " N5: 4124 " 1))))) 
COMMENT                            "Tth111I: 5 sites") 4 5 "GACNNNGTC" "CTGNNNCAG"))) 
COMMENT                   "Restriction/Methylation Map")))) 
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COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
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COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
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FEATURES             Location/Qualifiers
     misc_feature    516..1404
                     /vntifkey="21"
                     /label=psi+\extended\packaging\signal
     promoter        1..513
                     /vntifkey="29"
                     /label=5'\MSCV-PCMV\LTR
     CDS             1479..2444
                     /vntifkey="4"
                     /label=birA
                     /note="E. coli biotin protein ligase"
     misc_marker     1443..1466
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      3733..4245
                     /vntifkey="43"
                     /label=3'\MSCV-PCMV\LTR
     promoter        2506..3013
                     /vntifkey="29"
                     /label=mPGK1\promoter
     CDS             3033..3632
                     /vntifkey="4"
                     /label=puromycin\resistance
     CDS             complement(5576..6436)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(4633..5035)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(4458..4541)
                     /vntifkey="30"
                     /label=lac\promoter
BASE COUNT     1687 a      2011 c      1986 g      1686 t 
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggttaaactt aagcttacca 
     1441 tggattacaa ggacgatgac gataaggtac ccggatccat gaaggataac accgtgccac 
     1501 tgaaattgat tgccctgtta gcgaacggtg aatttcactc tggcgagcag ttgggtgaaa 
     1561 cgctgggaat gagccgggcg gctattaata aacacattca gacactgcgt gactggggcg 
     1621 ttgatgtctt taccgttccg ggtaaaggat acagcctgcc tgagcctatc cagttactta 
     1681 atgctaaaca gatattgggt cagctggatg gcggtagtgt agccgtgctg ccagtgattg 
     1741 actccacgaa tcagtacctt cttgatcgta tcggagagct taaatcgggc gatgcttgca 
     1801 ttgcagaata ccagcaggct ggccgtggtc gccggggtcg gaaatggttt tcgccttttg 
     1861 gcgcaaactt atatttgtcg atgttctggc gtctggaaca aggcccggcg gcggcgattg 
     1921 gtttaagtct ggttatcggt atcgtgatgg cggaagtatt acgcaagctg ggtgcagata 
     1981 aagttcgtgt taaatggcct aatgacctct atctgcagga tcgcaagctg gcaggcattc 
     2041 tggtggagct gactggcaaa actggcgatg cggcgcaaat agtcattgga gccgggatca 
     2101 acatggcaat gcgccgtgtt gaagagagtg tcgttaatca ggggtggatc acgctgcagg 
     2161 aagcggggat caatctcgat cgtaatacgt tggcggccat gctaatacgt gaattacgtg 
     2221 ctgcgttgga actcttcgaa caagaaggat tggcacctta tctgtcgcgc tgggaaaagc 
     2281 tggataattt tattaatcgc ccagtgaaac ttatcattgg tgataaagaa atatttggca 
     2341 tttcacgcgg aatagacaaa cagggggctt tattacttga gcaggatgga ataataaaac 
     2401 cctggatggg cggtgaaata tccctgcgta gtgcagaaaa ataagaattc tgcagatatc 
     2461 cagcacagtg gcggccgctc gagtctagag ggcccgttta acgaattcta ccgggtaggg 
     2521 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg 
     2581 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg 
     2641 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc 
     2701 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact 
     2761 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc 
     2821 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc 
     2881 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc 
     2941 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct 
     3001 ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc 
     3061 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 
     3121 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 
     3181 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 
     3241 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 
     3301 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 
     3361 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 
     3421 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 
     3481 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 
     3541 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 
     3601 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga 
     3661 ccgaaaggag cgcacgaccc catgcatcga taaaataaaa gattttattt agtctccaga 
     3721 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat 
     3781 tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac 
     3841 agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc 
     3901 tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac 
     3961 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta 
     4021 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag 
     4081 agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc 
     4141 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga 
     4201 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatgggt aacagtttct 
     4261 tgaagttgga gaacaacatt ctgagggtag gagtcgaata ttaagtaatc ctgactcaat 
     4321 tagccactgt tttgaatcca catactccaa tactcctgaa atagttcatt atggacagcg 
     4381 cagaagagct ggggagaatt aattcgtaat catggtcata gctgtttcct gtgtgaaatt 
     4441 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 
     4501 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 
     4561 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 
     4621 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     4681 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 
     4741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
     4801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
     4861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
     4921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
     4981 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 
     5041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     5101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     5161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     5221 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 
     5281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     5341 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     5401 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     5461 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
     5521 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
     5581 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
     5641 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
     5701 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
     5761 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
     5821 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
     5881 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
     5941 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
     6001 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
     6061 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
     6121 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
     6181 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
     6241 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
     6301 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
     6361 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
     6421 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 
     6481 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 
     6541 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 
     6601 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 
     6661 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 
     6721 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 
     6781 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 
     6841 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact 
     6901 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 
     6961 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 
     7021 cgacggcgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg 
     7081 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc 
     7141 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat 
     7201 gccggccacg atgcgtccgg cgtagaggcg attagtccaa tttgttaaag acaggatatc 
     7261 agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga 
     7321 gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa 
//
