LOCUS       pPLc28LIG8              4708 bp    DNA     circular      7-SEP-2009
DEFINITION  LMBP 574.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 574
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 7-SEP-2009
            
            
            
COMMENT     VNTNAME|pPLc28LIG8|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 4 360 0 755 0 1274 0 4707 
COMMENT            0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 16 0 2003 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 2 1342 0 3909 0 0 "")) 
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COMMENT        #5=(CFSignal (CObList) "pMB1 ori" 33 0 1 2293 2695 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3236 4096 0 
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COMMENT        #7=(CFSignal (CObList) "PL promoter" 30 0 0 4462 4592 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "5' UTR of phage T4 ligase, incl. RBS" 32 0 0 18 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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FEATURES             Location/Qualifiers
     CDS             538..2001
                     /vntifkey="4"
                     /label=phage\T4\ligase
     rep_origin      complement(2293..2695)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3236..4096)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        4462..4592
                     /vntifkey="30"
                     /label=PL\promoter
     RBS             18..537
                     /vntifkey="32"
                     /label=5'\UTR\of\phage\T4\ligase,\incl.\RBS
BASE COUNT     1386 a       987 c      1072 g      1263 t 
ORIGIN
        1 ttccggatcc ggccaagctt ttttcaccaa aggaaaataa agaaagtttt gatagtgaaa 
       61 aagctataaa agatgtaatt gaaaagatga ttaaaaacga ttcttttcct tggaacacta 
      121 cctggagaac tcctggattt aatccttata atccatatca tccatatcag acgcatccgt 
      181 ttcagacatg gaactatatt aagcccggcg gtatagagta tttgtataat agacctactt 
      241 gtggtgataa tattttccaa ggagcattct aatgtttgtt gttcacacta tttatgaaaa 
      301 tgaaggtaat actacacgtg attacggtca cgtaaatcaa ttttttagat gcaatccaga 
      361 attccgagct caaaaagacg aacgaatttt taaaaaatgt gtagagcaag gtttcattta 
      421 tatcaagcac tggatgcaag gaaataaagt tagaaccacg taccacaggt ctttgactga 
      481 gcttaatgat gaattgattt ataatagagc tgtaaaccaa actctaaagg atgaacaatg 
      541 attcttaaaa ttctgaacga aatagcatct attggttcaa ctaaacagaa gcaagcaatt 
      601 cttgaaaaga ataaagataa tgaattgctt aaacgagtat atcgtctgac ttattctcgt 
      661 gggttacagt attatatcaa gaaatggcct aaacctggta ttgctaccca gagttttgga 
      721 atgttgactc ttaccgatat gcttgacttc attgaattca cattagctac tcggaaattg 
      781 actggaaatg cagcaattga ggaattaact ggatatatca ccgatggtaa aaaagatgat 
      841 gttgaagttt tgcgtcgagt gatgatgcga gaccttgaat gtggtgcttc agtatctatt 
      901 gcaaacaaag tttggccagg tttaattcct gaacaacctc aaatgctcgc aagttcttat 
      961 gatgaaaaag gcattaataa gaatatcaaa tttccagcct ttgctcagtt aaaagctgat 
     1021 ggagctcggt gttttgctga agttagaggt gatgaattag atgatgttcg tcttttatca 
     1081 cgagctggta atgaatatct aggattagat cttcttaagg aagagttaat taaaatgacc 
     1141 gctgaagccc gccagattca tccagaaggt gtgttgattg atggcgaatt ggtataccat 
     1201 gagcaagtta aaaaggagcc agaaggccta gattttcttt ttgatgctta tcctgaaaac 
     1261 agtaaagcta aagaattcgc cgaagtagct gaatcacgta ctgcttctaa tggaatcgcc 
     1321 aataaatctt taaagggaac catttctgaa aaagaagcac aatgcatgaa gtttcaggtc 
     1381 tgggattatg tcccgttggt agaaatatac agtcttcctg catttcgttt gaaatatgat 
     1441 gtacgttttt ctaaactaga acaaatgaca tctggatatg ataaagtaat tttaattgaa 
     1501 aaccaggtag taaataacct agatgaagct aaggtaattt ataaaaagta tattgaccaa 
     1561 ggtcttgaag gtattattct caaaaatatc gatggattat gggaaaatgc tcgttcaaaa 
     1621 aatctttata aatttaaaga agtaattgat gttgatttaa aaattgtagg aatttatcct 
     1681 caccgtaaag accctactaa agcgggtgga tttattcttg agtcagagtg tggaaaaatt 
     1741 aaggtaaatg ctggttcagg cttaaaagat aaagccggtg taaaatcgca tgaacttgac 
     1801 cgtactcgca ttatggaaaa ccaaaattat tatattggaa aaattctaga gtgcgaatgc 
     1861 aacggttggt taaaatctga tggccgcact gattacgtta aattatttct tccgattgcg 
     1921 attcgtttac gtgaagataa aactaaagct aatacattcg aagatgtatt tggtgatttt 
     1981 catgaggtaa ctggtctatg aaagcttggc tgcctcgcgc gtttcggtga tgacggtgaa 
     2041 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 
     2101 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg 
     2161 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga 
     2221 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 
     2281 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     2341 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 
     2401 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
     2461 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
     2521 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
     2581 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
     2641 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 
     2701 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     2761 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     2821 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     2881 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 
     2941 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     3001 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     3061 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     3121 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
     3181 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
     3241 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
     3301 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
     3361 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
     3421 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
     3481 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
     3541 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
     3601 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
     3661 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
     3721 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
     3781 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
     3841 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
     3901 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
     3961 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
     4021 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
     4081 aatgttgaat actcatactc ttcctttttc aatattatgt aagcagacag ttttattgtt 
     4141 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg 
     4201 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac 
     4261 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac 
     4321 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcc 
     4381 tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgccggtg atgccggcca 
     4441 cgatgcgtcc ggcgtagagg atctctcacc taccaaacaa tgcccccctg caaaaaataa 
     4501 attcatataa aaaacataca gataaccatc tgcggtgata aattatctct ggcggtgttg 
     4561 acataaatac cactggcggt gatactgagc acatcagcag gacgcactga ccaccatgaa 
     4621 ggtgacgctc ttaaaattaa gccctgaaga agggcagcat tcaaagcaga aggctttggg 
     4681 gtgtgtgata cgaaacgaag cattggaa 
//
