LOCUS       pIB1                    5270 bp    DNA     circular      1-DEC-2009
DEFINITION  LMBP 5767.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5767
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 01-DEC-2009
            
COMMENT     VNTDATE|512929442|
COMMENT     VNTNAME|pIB1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pIB1" 0 5270 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 1 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BstXI" "SacI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 520 779 1 5270 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #0=(CFSignal (CObList) "ampicillin resistance" 4 0 1 1556 2416 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #1=(CFSignal (CObList) "pMB1 ori" 33 0 1 613 1015 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #2=(CFSignal (CObList) "AOX1 terminator" 43 0 0 520 779 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #3=(CFSignal (CObList) "Pp HIS4" 4 0 0 2516 5047 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #4=(CFSignal (CObList) "HIS4 terminator" 43 0 0 5048 5088 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "Last modified: 01-DEC-2009" 2 0 0 0 0 0 
COMMENT            (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #6=(CFSignal (CObList) "LMBP 5767" 2 0 0 0 0 0 (CStringList) 
COMMENT            (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #7=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT            (CObjectList 
COMMENT             #8=(CRefLinePar 
COMMENT                 (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pIB1" 2) 5 "" 0 4) 
COMMENT             #9=(CFolderPar 
COMMENT                 (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                  (CObjectList 
COMMENT                   #10=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "DNA Plasmid 'pIB1'" 1) 
COMMENT                   #11=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Local object" 
COMMENT                        1) 
COMMENT                   #12=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "Created: 03/16/09 04:24" 1) 
COMMENT                   #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "Last Modified: 12/01/09 10:35" 1) 
COMMENT                   #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "length: 5270 bp" 1) 
COMMENT                   #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "storage type: Basic" 1) 
COMMENT                   #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "form: Circular" 1))) "General Description") 
COMMENT             #17=(CFolderPar 
COMMENT                  (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                   (CObjectList 
COMMENT                    #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Original Source Database: GenBank" 1) 
COMMENT                    #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Modification Date in the Original DB: 16-MAR-2009" 1))) 
COMMENT                  "Standard Fields") 
COMMENT             #20=(CRefLinePar 
COMMENT                  (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                  0 0) 
COMMENT             #21=(CFolderPar 
COMMENT                  (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                   (CObjectList 
COMMENT                    #22=(CFolderPar 
COMMENT                         (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                          (CObjectList 
COMMENT                           #23=(CLinePar (CParagraph 0 (15 #5# 0) 1 2 2 0 180) 
COMMENT                                "" 1))) "Last modified: 01-DEC-2009") 
COMMENT                    #24=(CFolderPar 
COMMENT                         (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                          (CObjectList 
COMMENT                           #25=(CLinePar (CParagraph 0 (15 #6# 0) 1 2 2 0 180) 
COMMENT                                "" 1))) "LMBP 5767"))) "Annotations") 
COMMENT             #26=(CFolderPar 
COMMENT                  (CGroupPar (CParagraph 12 (6 0) 1 2 0 0 178) 
COMMENT                   (CObjectList 
COMMENT                    #27=(CFolderPar 
COMMENT                         (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                          (CObjectList 
COMMENT                           #28=(CFolderPar 
COMMENT                                (CGroupPar 
COMMENT                                 (CParagraph 1556 (3 #0# 0) 1 2 2 0 327) 
COMMENT                                 (CObjectList 
COMMENT                                  #29=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 1556  End: 2416 (Complementary)" 
COMMENT                                       1))) "ampicillin resistance") 
COMMENT                           #30=(CFolderPar 
COMMENT                                (CGroupPar 
COMMENT                                 (CParagraph 2516 (3 #3# 0) 1 2 2 0 327) 
COMMENT                                 (CObjectList 
COMMENT                                  #31=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 2516  End: 5047" 1) 
COMMENT                                  #32=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Pichia pastoris histidinol dehydrogenase HIS4" 
COMMENT                                       1))) "Pp HIS4"))) "CDS (2 total)") 
COMMENT                    #33=(CFolderPar 
COMMENT                         (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                          (CObjectList 
COMMENT                           #34=(CFolderPar 
COMMENT                                (CGroupPar 
COMMENT                                 (CParagraph 613 (3 #1# 0) 1 2 2 0 194) 
COMMENT                                 (CObjectList 
COMMENT                                  #35=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 613   End: 1015 (Complementary)" 
COMMENT                                       1))) "pMB1 ori"))) 
COMMENT                         "Replication Origin (1 total)") 
COMMENT                    #36=(CFolderPar 
COMMENT                         (CGroupPar (CParagraph 43 (7 43 0) 1 2 1 0 178) 
COMMENT                          (CObjectList 
COMMENT                           #37=(CFolderPar 
COMMENT                                (CGroupPar 
COMMENT                                 (CParagraph 520 (3 #2# 0) 1 2 2 0 194) 
COMMENT                                 (CObjectList 
COMMENT                                  #38=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 520   End: 779 " 1))) 
COMMENT                                "AOX1 terminator") 
COMMENT                           #39=(CFolderPar 
COMMENT                                (CGroupPar 
COMMENT                                 (CParagraph 5048 (3 #4# 0) 1 2 2 0 194) 
COMMENT                                 (CObjectList 
COMMENT                                  #40=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 5048  End: 5088" 1) 
COMMENT                                  #41=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Pischia pastoris HIS4 terminator" 1))) 
COMMENT                                "HIS4 terminator"))) "Terminator (2 total)"))) 
COMMENT                  "Feature Map") 
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COMMENT                   (CObjectList 
COMMENT                    #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "   Similarity between ambiguous nucleotides (if any)" 
COMMENT                         1) 
COMMENT                    #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "is calculated as an AVERAGE similarity" 1))) 
COMMENT                  "Motifs (both strands)")))) 
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COMMENT               "@F bp (molecule @L bp)" 0) 
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FEATURES             Location/Qualifiers
     terminator      5048..5088
                     /vntifkey="43"
                     /label=HIS4\terminator
                     /note="Pischia pastoris HIS4 terminator"
     CDS             complement(1556..2416)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(613..1015)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      520..779
                     /vntifkey="43"
                     /label=AOX1\terminator
     CDS             2516..5047
                     /vntifkey="4"
                     /label=Pp\HIS4
                     /note="Pichia pastoris histidinol dehydrogenase HIS4"
BASE COUNT     1367 a      1199 c      1265 g      1439 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 
      421 ccactagtct cgagctgcag gcatgcaagc ttcttagaca tgactgttcc tcagttcaag 
      481 ttgggcactt acgagaagac cggtcttgct agattctaat caagaggatg tcagaatgcc 
      541 atttgcctga gagatgcagg cttcattttt gatacttttt tatttgtaac ctatatagta 
      601 taggattttt tttgtcattt tgtttcttct cgtacgagct tgctcctgat cagcctatct 
      661 cgcagctgat gaatatcttg tggtaggggt ttgggaaaat cattcgagtt tgatgttttt 
      721 cttggtattt cccacacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
      781 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
      841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
      901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
      961 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg 
     1021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     1081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     1141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     1201 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 
     1261 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     1321 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     1381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     1441 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
     1501 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
     1561 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
     1621 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
     1681 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
     1741 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
     1801 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
     1861 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
     1921 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
     1981 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
     2041 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
     2101 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
     2161 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
     2221 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
     2281 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
     2341 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
     2401 aatgttgaat actcatactc ttcctttttc aatgatctcc tgatgactga ctcactgata 
     2461 ataaaaatac ggcttcagaa tttctcaaga ctacactcac tgtccgactt caagtatgac 
     2521 atttcccttg ctacctgcat acgcaagtgt tgcagagttt gataattcct tgagtttggt 
     2581 aggaaaagcc gtgtttccct atgctgctga ccagctgcac aacctgatca agttcactca 
     2641 atcgactgag cttcaagtta atgtgcaagt tgagtcatcc gttacagagg accaatttga 
     2701 ggagctgatc gacaacttgc tcaagttgta caataatggt atcaatgaag tgattttgga 
     2761 cctagatttg gcagaaagag ttgtccaaag gatgatccca ggcgctaggg ttatctatag 
     2821 gaccctggtt gataaagttg catccttgcc cgctaatgct agtatcgctg tgcctttttc 
     2881 ttctccactg ggcgatttga aaagtttcac taatggcggt agtagaactg tttatgcttt 
     2941 ttctgagacc gcaaagttgg tagatgtgac ttccactgtt gcttctggta taatccccat 
     3001 tattgatgct cggcaattga ctactgaata cgaactttct gaagatgtca aaaagttccc 
     3061 tgtcagtgaa attttgttgg cgtctttgac tactgaccgc cccgatggtc tattcactac 
     3121 tttggtggct gactcttcta attactcgtt gggcctggtg tactcgtcca aaaagtctat 
     3181 tccggaggct ataaggacac aaactggagt ctaccaatct cgtcgtcacg gtttgtggta 
     3241 taaaggtgct acatctggag caactcaaaa gttgctgggt atcgaattgg attgtgatgg 
     3301 agactgcttg aaatttgtgg ttgaacaaac aggtgttggt ttctgtcact tggaacgcac 
     3361 ttcctgtttt ggccaatcaa agggtcttag agccatggaa gccaccttgt gggatcgtaa 
     3421 gagcaatgct ccagaaggtt cttataccaa acggttattt gacgacgaag ttttgttgaa 
     3481 cgctaaaatt agggaggaag ctgatgaact tgcagaagct aaatccaagg aagatatagc 
     3541 ctgggaatgt gctgacttat tttattttgc attagttaga tgtgccaagt acggcgtgac 
     3601 gttggacgag gtggagagaa acctggatat gaagtcccta aaggtcacta gaaggaaagg 
     3661 agatgccaag ccaggataca ccaaggaaca acctaaagaa gaatccaaac ctaaagaagt 
     3721 cccttctgaa ggtcgtattg aattgtgcaa aattgacgtt tctaaggcct cctcacaaga 
     3781 aattgaagat gcccttcgtc gtcctatcca gaaaacggaa cagattatgg aattagtcaa 
     3841 accaattgtc gacaatgttc gtcaaaatgg tgacaaagcc cttttagaac taactgccaa 
     3901 gtttgatgga gtcgctttga agacacctgt gttagaagct cctttcccag aggaacttat 
     3961 gcaattgcca gataacgtta agagagccat tgatctctct atagataacg tcaggaaatt 
     4021 ccatgaagct caactagcgg agacgttgca agttgagact tgccctggtg tagtctgctc 
     4081 tcgttttgca agacctattg agaaagttgg cctctatatt cctggtggaa ccgcaattct 
     4141 gccttccact tccctgatgc tgggtgttcc tgccaaagtt gctggttgca aagaaattgt 
     4201 ttttgcatct ccacctaaga aggatggcac ccttacccca gaagtcatct acgttgccca 
     4261 caaggttggt gctaagtgta tcgtgctagc aggaggcgcc caggcagtag ctgctatggc 
     4321 ttacggaaca gaaactgttc ctaagtgtga caaaatattt ggtccaggaa accagttcgt 
     4381 tactgctgcc aagatgatgg ttcaaaatga cacatcagcc ctgtgtagta ttgacatgcc 
     4441 tgctgggcct tctgaagttc tagttattgc tgataaatac gctgatccag atttcgttgt 
     4501 ctcagacctt ctgtctcaag ctgaacatgg tattgattcc caggtgattc tgttggctgt 
     4561 cgatatgaca gacaaggagc ttgccagaat tgaagatgct gttcacaacc aagctgtgca 
     4621 gttgccaagg gttgaaattg tacgcaagtg tattgcacac tctacaaccc tatcggttgc 
     4681 aacctacgag caggctttgg aaatgtccaa tcagtacgct cctgaacact tgatcctgca 
     4741 aatcgagaat gcttcttatg ttgatcaagt acaacacgct ggatctgtgt ttgttggtgc 
     4801 ctactctcca gagagttgtg gagattactc ctccggcacc aaccacactt tgccaacgta 
     4861 cggatatgcc cgtcaataca gcggagttaa cactgcaacc ttccagaagt tcatcacttc 
     4921 acaagacgta actcctgagg gactgaaaca tattggccaa gcagtgatgg atctggctgc 
     4981 tgttgaaggt ctagatgctc accgcaatgc tgttaaggtt cgtatggaga aactgggact 
     5041 tatttaatta tttagagatt ttaacttaca tttagattcg atagatcatt attgaagcat 
     5101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 
     5161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 
     5221 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 
//
