LOCUS       pIB4                    5796 bp    DNA     circular     30-MAY-2011
DEFINITION  LMBP 5770.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5770
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 30-MAY-2011
            
            
            
            
            
COMMENT     VNTDATE|512930788|
COMMENT     VNTNAME|pIB4|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pIB4" 0 5796 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
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COMMENT         "PstI" "SacI" "SpeI" "XhoI" "SmaI" "SphI" "EcoRI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 922 927 1 5796 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 944 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 3 257 0 2276 0 4971 0 
COMMENT            0 "") 
COMMENT        #2=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 3 1511 0 3332 0 4826 0 0 
COMMENT            "") 
COMMENT        #3=(CRSite (CStringList) "PvuII" "cagctg" 4 0 2 1192 0 3141 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HincII" "gtyrac" 4 0 2 4377 0 5415 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 852 0 974 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 939 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 966 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 933 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 950 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 956 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 941 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "SphI" "gcatgc" 6 0 1 972 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 923 0 0 "")) 
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COMMENT        #15=(CFSignal (CObList) "pMB1 ori" 33 0 1 1262 1541 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2082 2942 0 
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COMMENT        #17=(CFSignal (CObList) "Pichia AOX1 promoter" 29 0 0 187 750 0 
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COMMENT        #18=(CFSignal (CObList) "5' UTR of AOX1" 21 0 0 751 916 0 (CStringList) 
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COMMENT        #19=(CFSignal (CObList) "Pichia AOX1 terminator" 43 0 0 1046 1261 0 
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COMMENT                          "Created: 03/16/09 04:46" 1) 
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COMMENT                          "Last Modified: 05/30/11 12:24" 1) 
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COMMENT                          "length: 5796 bp" 1) 
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COMMENT                                 "Pichia AOX1 terminator"))) 
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COMMENT                          "CTGCAG" "GACGTC") 
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COMMENT                          "ACTAGT" "TGATCA") 
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COMMENT                          "CCCGGG" "GGGCCC") 
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COMMENT        #336=(CSampFrag "pIB4" 1892 5415 "HincII" 1511 "HaeII" (LOGPEN 0 0 0) 0 
COMMENT              5.48015e-294) 
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COMMENT              2.36974e-316 1.02894e-314) 
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COMMENT              2.29794e-316 1.02894e-314) 
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COMMENT              1.05823e+034) 
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COMMENT              2.122e-314 4.94066e-324))))
FEATURES             Location/Qualifiers
     CDS             3042..5573
                     /vntifkey="4"
                     /label=Pichia\HIS4
     terminator      1046..1261
                     /vntifkey="43"
                     /label=Pichia\AOX1\terminator
     rep_origin      complement(1262..1541)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(2082..2942)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        187..750
                     /vntifkey="29"
                     /label=Pichia\AOX1\promoter
     misc_feature    751..916
                     /vntifkey="21"
                     /label=5'\UTR\of\AOX1
BASE COUNT     1528 a      1316 c      1331 g      1621 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatacgct cattccaatt ccttctatta ggctactaac accatgactt tattagcctg 
      241 tctatcctgg cccccctggc gaggtcatgt ttgtttattt ccgaatgcaa caagctccgc 
      301 attacacccg aacatcactc cagatgaggg ctttctgagt gtggggtcaa atagtttcat 
      361 gttcccaaat ggcccaaaac tgacagttta aacgctgtct tggaacctaa tatgacaaaa 
      421 gcgtgatctc atccaagatg aactaagttt ggttcgttga aatgctaacg gccagttggt 
      481 caaaaagaaa cttccaaaag tcgccatacc gtttgtcttg tttggtattg attgacgaat 
      541 gctcaaaaat aatctcatta atgcttagcg cagtctctct atcgcttctg aacccggtgg 
      601 cacctgtgcc gaaacgcaaa tggggaaaca acccgctttt tggatgatta tgcattgtcc 
      661 tccacattgt atgcttccaa gattctggtg ggaatactgc tgatagccta acgttcatga 
      721 tcaaaattta actgttctaa cccctacttg acaggcaata tataaacaga aggaagctgc 
      781 cctgtcttaa accttttttt ttatcatcat tattagctta ctttcataat tgcgactggt 
      841 tccaattgac aagcttttga ttttaacgac ttttaacgac aacttgagaa gatcaaaaaa 
      901 caactaatta ttcgaaacga ggaattcgag ctcggtaccc ggggatccac tagtctcgag 
      961 ctgcaggcat gcaagcttct tagacatgac tgttcctcag ttcaagttgg gcacttacga 
     1021 gaagaccggt cttgctagat tctaatcaag aggatgtcag aatgccattt gcctgagaga 
     1081 tgcaggcttc atttttgata cttttttatt tgtaacctat atagtatagg attttttttg 
     1141 tcattttgtt tcttctcgta cgagcttgct cctgatcagc ctatctcgca gctgatgaat 
     1201 atcttgtggt aggggtttgg gaaaatcatt cgagtttgat gtttttcttg gtatttccca 
     1261 cacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 
     1321 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 
     1381 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 
     1441 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 
     1501 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 
     1561 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 
     1621 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 
     1681 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 
     1741 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 
     1801 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 
     1861 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 
     1921 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 
     1981 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 
     2041 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 
     2101 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 
     2161 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 
     2221 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 
     2281 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 
     2341 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 
     2401 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 
     2461 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 
     2521 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 
     2581 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 
     2641 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 
     2701 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 
     2761 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 
     2821 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 
     2881 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 
     2941 atactcttcc tttttcaatg atctcctgat gactgactca ctgataataa aaatacggct 
     3001 tcagaatttc tcaagactac actcactgtc cgacttcaag tatgacattt cccttgctac 
     3061 ctgcatacgc aagtgttgca gagtttgata attccttgag tttggtagga aaagccgtgt 
     3121 ttccctatgc tgctgaccag ctgcacaacc tgatcaagtt cactcaatcg actgagcttc 
     3181 aagttaatgt gcaagttgag tcatccgtta cagaggacca atttgaggag ctgatcgaca 
     3241 acttgctcaa gttgtacaat aatggtatca atgaagtgat tttggaccta gatttggcag 
     3301 aaagagttgt ccaaaggatg atcccaggcg ctagggttat ctataggacc ctggttgata 
     3361 aagttgcatc cttgcccgct aatgctagta tcgctgtgcc tttttcttct ccactgggcg 
     3421 atttgaaaag tttcactaat ggcggtagta gaactgttta tgctttttct gagaccgcaa 
     3481 agttggtaga tgtgacttcc actgttgctt ctggtataat ccccattatt gatgctcggc 
     3541 aattgactac tgaatacgaa ctttctgaag atgtcaaaaa gttccctgtc agtgaaattt 
     3601 tgttggcgtc tttgactact gaccgccccg atggtctatt cactactttg gtggctgact 
     3661 cttctaatta ctcgttgggc ctggtgtact cgtccaaaaa gtctattccg gaggctataa 
     3721 ggacacaaac tggagtctac caatctcgtc gtcacggttt gtggtataaa ggtgctacat 
     3781 ctggagcaac tcaaaagttg ctgggtatcg aattggattg tgatggagac tgcttgaaat 
     3841 ttgtggttga acaaacaggt gttggtttct gtcacttgga acgcacttcc tgttttggcc 
     3901 aatcaaaggg tcttagagcc atggaagcca ccttgtggga tcgtaagagc aatgctccag 
     3961 aaggttctta taccaaacgg ttatttgacg acgaagtttt gttgaacgct aaaattaggg 
     4021 aggaagctga tgaacttgca gaagctaaat ccaaggaaga tatagcctgg gaatgtgctg 
     4081 acttatttta ttttgcatta gttagatgtg ccaagtacgg cgtgacgttg gacgaggtgg 
     4141 agagaaacct ggatatgaag tccctaaagg tcactagaag gaaaggagat gccaagccag 
     4201 gatacaccaa ggaacaacct aaagaagaat ccaaacctaa agaagtccct tctgaaggtc 
     4261 gtattgaatt gtgcaaaatt gacgtttcta aggcctcctc acaagaaatt gaagatgccc 
     4321 ttcgtcgtcc tatccagaaa acggaacaga ttatggaatt agtcaaacca attgtcgaca 
     4381 atgttcgtca aaatggtgac aaagcccttt tagaactaac tgccaagttt gatggagtcg 
     4441 ctttgaagac acctgtgtta gaagctcctt tcccagagga acttatgcaa ttgccagata 
     4501 acgttaagag agccattgat ctctctatag ataacgtcag gaaattccat gaagctcaac 
     4561 tagcggagac gttgcaagtt gagacttgcc ctggtgtagt ctgctctcgt tttgcaagac 
     4621 ctattgagaa agttggcctc tatattcctg gtggaaccgc aattctgcct tccacttccc 
     4681 tgatgctggg tgttcctgcc aaagttgctg gttgcaaaga aattgttttt gcatctccac 
     4741 ctaagaagga tggcaccctt accccagaag tcatctacgt tgcccacaag gttggtgcta 
     4801 agtgtatcgt gctagcagga ggcgcccagg cagtagctgc tatggcttac ggaacagaaa 
     4861 ctgttcctaa gtgtgacaaa atatttggtc caggaaacca gttcgttact gctgccaaga 
     4921 tgatggttca aaatgacaca tcagccctgt gtagtattga catgcctgct gggccttctg 
     4981 aagttctagt tattgctgat aaatacgctg atccagattt cgttgtctca gaccttctgt 
     5041 ctcaagctga acatggtatt gattcccagg tgattctgtt ggctgtcgat atgacagaca 
     5101 aggagcttgc cagaattgaa gatgctgttc acaaccaagc tgtgcagttg ccaagggttg 
     5161 aaattgtacg caagtgtatt gcacactcta caaccctatc ggttgcaacc tacgagcagg 
     5221 ctttggaaat gtccaatcag tacgctcctg aacacttgat cctgcaaatc gagaatgctt 
     5281 cttatgttga tcaagtacaa cacgctggat ctgtgtttgt tggtgcctac tctccagaga 
     5341 gttgtggaga ttactcctcc ggcaccaacc acactttgcc aacgtacgga tatgcccgtc 
     5401 aatacagcgg agttaacact gcaaccttcc agaagttcat cacttcacaa gacgtaactc 
     5461 ctgagggact gaaacatatt ggccaagcag tgatggatct ggctgctgtt gaaggtctag 
     5521 atgctcaccg caatgctgtt aaggttcgta tggagaaact gggacttatt taattattta 
     5581 gagattttaa cttacattta gattcgatag atcattattg aagcatttat cagggttatt 
     5641 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 
     5701 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 
     5761 cctataaaaa taggcgtatc acgaggccct ttcgtc 
//
