ID pFB-Neo-E-hA20 standard; circular DNA; ; 9033 BP. DE LMBP 5942. CC Accession number: LMBP 5942 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 30-JUL-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT rep_origin complement(7025..7427) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(7968..8828) FT /vntifkey="4" FT /label=ampicillin\resistance FT RBS 4555..5053 FT /vntifkey="32" FT /label=5'\UTR\of\EMC-POLYP\(IRES) FT /note="IRES of the encephalomyocarditis virus polyprotein gene" FT CDS 5225..6019 FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT promoter 169..760 FT /vntifkey="29" FT /label=5'\MMLV-LTR FT misc_feature 761..2046 FT /vntifkey="21" FT /label=psi+\extended\MMLV\packaging\signal FT terminator 6079..6672 FT /vntifkey="43" FT /label=3'\MMLV-LTR FT misc_marker 2077..2115 FT /vntifkey="22" FT /label=E-tag FT CDS 2125..4497 FT /vntifkey="4" FT /label=hA20 FT misc_feature 4498..4541 FT /vntifkey="21" FT /label=3'\UTR\of\hA20 SQ Sequence 9033 BP; 2165 A; 2520 C; 2309 G; 2039 t; gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc 60 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta 120 ccctattccc cacactcacc ggagccaaag ccgcgggaca tatacatgtg aaagacccca 180 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat 240 aactgagaat agaaaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc 300 caaagcggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa 360 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc 420 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga 480 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc 540 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac 600 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc 660 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc 720 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg 780 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta 840 tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta 900 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc 960 cggccgcaac cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg 1020 agtccaaaaa tcccgatcgt tttggactct ttggtgcacc ccccttagag gagggatatg 1080 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 1140 cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc 1200 tctgtctgac tgtgtttctg tatttgtctg aaaatatggg cccgggccag actgttacca 1260 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcagatcgct cacaaccagt 1320 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta 1380 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca 1440 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg 1500 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc 1560 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc 1620 gatcctccct ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga 1680 tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta 1740 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct 1800 ggagacctct ggcggcagcc taccaagaac aactggaccg accggtggta cctcaccctt 1860 accgagtcgg cgacacagtg tgggtccgcc gacaccagac taagaaccta gaacctcgct 1920 ggaaaggacc ttacacagtc ctgctgacca cccccaccgc cctcaaagta gacggcatcg 1980 cagcttggat acacgccgcc cacgtgaagg ctgccgaccc cgggggtgga ccatcctcta 2040 gactgccgga tcgaattgtc gacgaattcc accatgggtg cgccggtgcc gtatccggac 2100 ccgctggaac cgcgtgcggc cgcaatggct gaacaagtcc ttcctcaggc tttgtatttg 2160 agcaatatgc ggaaagctgt gaagatacgg gagagaactc cagaagacat ttttaaacct 2220 actaatggga tcattcatca ttttaaaacc atgcaccgat acacactgga aatgttcaga 2280 acttgccagt tttgtcctca gtttcgggag atcatccaca aagccctcat cgacagaaac 2340 atccaggcca ccctggaaag ccagaagaaa ctcaactggt gtcgagaagt ccggaagctt 2400 gtggcgctga aaacgaacgg tgacggcaat tgcctcatgc atgccacttc tcagtacatg 2460 tggggcgttc aggacacaga cttggtactg aggaaggcgc tgttcagcac gctcaaggaa 2520 acagacacac gcaactttaa attccgctgg caactggagt ctctcaaatc tcaggaattt 2580 gttgaaacgg ggctttgcta tgatactcgg aactggaatg atgaatggga caatcttatc 2640 aaaatggctt ccacagacac acccatggcc cgaagtggac ttcagtacaa ctcactggaa 2700 gaaatacaca tatttgtcct ttgcaacatc ctcagaaggc caatcattgt catttcagac 2760 aaaatgctaa gaagtttgga atcaggttcc aatttcgccc ctttgaaagt gggtggaatt 2820 tacttgcctc tccactggcc tgcccaggaa tgctacagat accccattgt tctcggctat 2880 gacagccatc attttgtacc cttggtgacc ctgaaggaca gtgggcctga aatccgagct 2940 gttccacttg ttaacagaga ccggggaaga tttgaagact taaaagttca ctttttgaca 3000 gatcctgaaa atgagatgaa ggagaagctc ttaaaagagt acttaatggt gatagaaatc 3060 cccgtccaag gctgggacca tggcacaact catctcatca atgccgcaaa gttggatgaa 3120 gctaacttac caaaagaaat caatctggta gatgattact ttgaacttgt tcagcatgag 3180 tacaagaaat ggcaggaaaa cagcgagcag gggaggagag aggggcacgc ccagaatccc 3240 atggaacctt ccgtgcccca gctttctctc atggatgtaa aatgtgaaac gcccaactgc 3300 cccttcttca tgtctgtgaa cacccagcct ttatgccatg agtgctcaga gaggcggcaa 3360 aagaatcaaa acaaactccc aaagctgaac tccaagccgg gccctgaggg gctccctggc 3420 atggcgctcg gggcctctcg gggagaagcc tatgagccct tggcgtggaa ccctgaggag 3480 tccactgggg ggcctcattc ggccccaccg acagcaccca gcccttttct gttcagtgag 3540 accactgcca tgaagtgcag gagccccggc tgccccttca cactgaatgt gcagcacaac 3600 ggattttgtg aacgttgcca caacgcccgg caacttcacg ccagccacgc cccagaccac 3660 acaaggcact tggatcccgg gaagtgccaa gcctgcctcc aggatgttac caggacattt 3720 aatgggatct gcagtacttg cttcaaaagg actacagcag aggcctcctc cagcctcagc 3780 accagcctcc ctccttcctg tcaccagcgt tccaagtcag atccctcgcg gctcgtccgg 3840 agcccctccc cgcattcttg ccacagagct ggaaacgacg cccctgctgg ctgcctgtct 3900 caagctgcac ggactcctgg ggacaggacg gggacgagca agtgcagaaa agccggctgc 3960 gtgtattttg ggactccaga aaacaagggc ttttgcacac tgtgtttcat cgagtacaga 4020 gaaaacaaac attttgctgc tgcctcaggg aaagtcagtc ccacagcgtc caggttccag 4080 aacaccattc cgtgcctggg gagggaatgc ggcacccttg gaagcaccat gtttgaagga 4140 tactgccaga agtgtttcat tgaagctcag aatcagagat ttcatgaggc caaaaggaca 4200 gaagagcaac tgagatcgag ccagcgcaga gatgtgcctc gaaccacaca aagcacctca 4260 aggcccaagt gcgcccgggc ctcctgcaag aacatcctgg cctgccgcag cgaggagctc 4320 tgcatggagt gtcagcatcc caaccagagg atgggccctg gggcccaccg gggtgagcct 4380 gcccccgaag acccccccaa gcagcgttgc cgggcccccg cctgtgatca ttttggcaat 4440 gccaagtgca acggctactg caacgaatgc tttcagttca agcagatgta tggctaaccg 4500 gaaacaggtg ggtcacctcc tgcaagaagt ggggcctcga gcggccgcga tccggttatt 4560 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt 4620 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt 4680 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct 4740 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt 4800 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt 4860 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa 4920 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta 4980 gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa 5040 acacgatcga taatatggaa caaaaactta tttctgaaga agacttggac accaaacttt 5100 cctgccgctc gatttctcca cccaggtgaa ctcctccctc acctccccga cggggcgagg 5160 ctccatggct gccccctcgc tgcacccgtc cctggtgcca cgcggttcca tgggatcgtt 5220 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 5280 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 5340 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 5400 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 5460 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 5520 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 5580 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 5640 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 5700 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc 5760 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 5820 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 5880 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 5940 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 6000 tcttgacgag ttcttctgag cgggactctg gggttcgata aaataaaaga ttttatttag 6060 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt 6120 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag 6180 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc 6240 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc 6300 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc 6360 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa 6420 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc 6480 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc 6540 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga 6600 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg 6660 gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc 6720 caccaccggg aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg 6780 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 6840 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc 6900 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg 6960 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 7020 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 7080 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 7140 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 7200 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 7260 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 7320 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 7380 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 7440 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 7500 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 7560 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 7620 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 7680 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 7740 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 7800 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 7860 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 7920 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 7980 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 8040 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 8100 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 8160 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 8220 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 8280 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 8340 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 8400 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 8460 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 8520 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 8580 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 8640 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 8700 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 8760 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 8820 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 8880 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 8940 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 9000 aataggcgta tcacgaggcc ctttcgtctt caa 9033 //