LOCUS       pFLAG-p65-S276A         6306 bp    DNA     circular     11-SEP-2014
DEFINITION  LMBP 6089.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6089
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-SEP-2014
COMMENT     VNTDATE|511029702|
COMMENT     VNTNAME|pFLAG-p65-S276A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pFLAG-p65-S276A" 0 6306 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "EcoRV" "HindIII" "NdeI" "PvuI" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 1 6306 1 6306 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2618 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 957 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NdeI" "catatg" 3 0 2 568 0 1874 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 42 0 1841 0 5303 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 3635 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "f1 ori" 33 0 0 5851 6306 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5851..6306") 
COMMENT        #6=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 318 901 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "318..901") 
COMMENT        #7=(CFSignal (CObList) "SV40 early promoter" 29 0 0 3289 3570 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3289..3570") 
COMMENT        #8=(CFSignal (CObList) "SV40 ori" 33 0 0 3527 3602 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3527..3602") 
COMMENT        #9=(CFSignal (CObList) "ampicillin resistance" 4 0 1 4859 5719 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(4859..5719)") 
COMMENT        #10=(CFSignal (CObList) "T7 promoter" 30 0 1 3668 3684 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3668..3684)") 
COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 3916 4318 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3916..4318)") 
COMMENT        #12=(CFSignal (CObList) "FLAG epitope" 22 0 0 935 958 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "935..958") 
COMMENT        #13=(CFSignal (CObList) "lac promoter" 30 0 1 3741 3824 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3741..3824)") 
COMMENT        #14=(CFSignal (CObList) "hNF-kappaB p65" 4 0 0 962 2614 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "962..2614") 
COMMENT        #15=(CFSignal (CObList) "hGH polyA" 43 0 0 2644 3123 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2644..3123") 
COMMENT        #16=(CFSignal (CObList) "S276A" 62 0 0 1784 1786 0 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "Last modified: 11-SEP-2014" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pFLAG-p65-S276A'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 02/26/09 04:41" 1) 
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FEATURES             Location/Qualifiers
     rep_origin      5851..6306
                     /vntifkey="33"
                     /label=f1\ori
     promoter        318..901
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        3289..3570
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      3527..3602
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             complement(4859..5719)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        complement(3668..3684)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      complement(3916..4318)
                     /vntifkey="33"
                     /label=pMB1\ori
     misc_marker     935..958
                     /vntifkey="22"
                     /label=FLAG\epitope
     promoter        complement(3741..3824)
                     /vntifkey="30"
                     /label=lac\promoter
     CDS             962..2614
                     /vntifkey="4"
                     /label=hNF-kappaB\p65
     terminator      2644..3123
                     /vntifkey="43"
                     /label=hGH\polyA
     mutation        1784..1786
                     /vntifkey="62"
                     /label=S276A
                     /note="WT: TCC"
BASE COUNT     1463 a      1765 c      1556 g      1522 t 
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa 
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta 
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg 
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 
      901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct 
      961 tgacgaactg ttccccctca tcttcccggc agagccagcc caggcctctg gcccctatgt 
     1021 ggagatcatt gagcagccca agcagcgggg catgcgcttc cgctacaagt gcgaggggcg 
     1081 ctccgcgggc agcatcccag gcgagaggag cacagatacc accaagaccc accccaccat 
     1141 caagatcaat ggctacacag gaccagggac agtgcgcatc tccctggtca ccaaggaccc 
     1201 tcctcaccgg cctcaccccc acgagcttgt aggaaaggac tgccgggatg gcttctatga 
     1261 ggctgagctc tgcccggacc gctgcatcca cagtttccag aacctgggaa tccagtgtgt 
     1321 gaagaagcgg gacctggagc aggctatcag tcagcgcatc cagaccaaca acaacccctt 
     1381 ccaagttcct atagaagagc agcgtgggga ctacgacctg aatgctgtgc ggctctgctt 
     1441 ccaggtgaca gtgcgggacc catcaggcag gcccctccgc ctgccgcctg tccttcctca 
     1501 tcccatcttt gacaatcgtg cccccaacac tgccgagctc aagatctgcc gagtgaaccg 
     1561 aaactctggc agctgcctcg gtggggatga gatcttccta ctgtgtgaca aggtgcagaa 
     1621 agaggacatt gaggtgtatt tcacgggacc aggctgggag gcccgaggct ccttttcgca 
     1681 agctgatgtg caccgacaag tggccattgt gttccggacc cctccctacg cagaccccag 
     1741 cctgcaggct cctgtgcgtg tctccatgca gctgcggcgg cctgccgacc gggagctcag 
     1801 tgagcccatg gaattccagt acctgccaga tacagacgat cgtcaccgga ttgaggagaa 
     1861 acgtaaaagg acatatgaga ccttcaagag catcatgaag aagagtcctt tcagcggacc 
     1921 caccgacccc cggcctccac ctcgacgcat tgctgtgcct tcccgcagct cagcttctgt 
     1981 ccccaagcca gcaccccagc cctatccctt tacgtcatcc ctgagcacca tcaactatga 
     2041 tgagtttccc accatggtgt ttccttctgg gcagatcagc caggcctcgg ccttggcccc 
     2101 ggcccctccc caagtcctgc cccaggctcc agcccctgcc cctgctccag ccatggtatc 
     2161 agctctggcc caggccccag cccctgtccc agtcctagcc ccaggccctc ctcaggctgt 
     2221 ggccccacct gcccccaagc ccacccaggc tggggaagga acgctgtcag aggccctgct 
     2281 gcagctgcag tttgatgatg aagacctggg ggccttgctt ggcaacagca cagacccagc 
     2341 tgtgttcaca gacctggcat ccgtcgacaa ctccgagttt cagcagctgc tgaaccaggg 
     2401 catacctgtg gccccccaca caactgagcc catgctgatg gagtaccctg aggctataac 
     2461 tcgcctagtg acaggggccc agaggccccc cgacccagct cctgctccac tgggggcccc 
     2521 ggggctcccc aatggcctcc tttcaggaga tgaagacttc tcctccattg cggacatgga 
     2581 cttctcagcc ctgctgagtc agatcagctc ctaagatatc ggtaccagtc gactctagag 
     2641 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc 
     2701 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag 
     2761 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg 
     2821 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa 
     2881 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc 
     2941 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag 
     3001 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac 
     3061 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc 
     3121 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct 
     3181 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt 
     3241 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcttggct gtggaatgtg 
     3301 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 
     3361 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 
     3421 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 
     3481 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 
     3541 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 
     3601 ttttttggag gcctaggctt ttgcaaaaag ctcctcgagg aactgaaaaa ccagaaagtt 
     3661 aattccctat agtgagtcgt attaaattcg taatcatgtc atagctgttt cctgtgtgaa 
     3721 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 
     3781 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 
     3841 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 
     3901 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 
     3961 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 
     4021 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 
     4081 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
     4141 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
     4201 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
     4261 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
     4321 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
     4381 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
     4441 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
     4501 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 
     4561 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
     4621 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 
     4681 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 
     4741 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 
     4801 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 
     4861 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     4921 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     4981 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
     5041 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     5101 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     5161 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     5221 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     5281 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     5341 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     5401 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     5461 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     5521 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     5581 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     5641 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     5701 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     5761 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 
     5821 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg 
     5881 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 
     5941 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 
     6001 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 
     6061 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 
     6121 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 
     6181 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 
     6241 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta 
     6301 caattt 
//
