LOCUS       pEF6-mTRAF2-cMyc        7254 bp    DNA     circular     27-JUL-2018
DEFINITION  LMBP 6140.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6140
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 27-JUL-2018
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12391794|
COMMENT     VNTDBDATE|-12394656|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEF6-mTRAF2-cMyc|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-mTRAF2-cMyc" 0 7254 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BglII" "EcoRI" "NcoI" "NotI" "XbaI" "BamHI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
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COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3061 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 2619 0 4161 0 4381 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 1611 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 3122 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1604 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "T7 promoter" 30 0 0 1546 1562 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1546..1562") 
COMMENT        #7=(CFSignal (CObList) "His-tag" 22 0 0 3181 3198 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3181..3198") 
COMMENT        #8=(CFSignal (CObList) "BGH polyA" 43 0 0 3227 3451 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3227..3451") 
COMMENT        #9=(CFSignal (CObList) "f1 ori" 33 0 0 3497 3925 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3497..3925") 
COMMENT        #10=(CFSignal (CObList) "blasticidin resistance" 4 0 0 4382 4780 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4382..4780") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4938 5012 0 
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COMMENT        #12=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4938 5012 0 
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COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 5326 5717 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "SV40 early promoter" 29 0 0 3930 4211 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3930..4211") 
COMMENT        #15=(CFSignal (CObList) "SV40 ori" 33 0 0 4168 4243 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4168..4243") 
COMMENT        #16=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6258 7118 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(6258..7118)") 
COMMENT        #17=(CFSignal (CObList) "hEF1a promoter" 29 0 0 386 1529 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "386..1529") 
COMMENT        #18=(CFSignal (CObList) "Myc epitope" 22 0 0 3136 3165 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3136..3165") 
COMMENT        #19=(CFSignal (CObList) "synthetic EM7 promoter" 30 0 0 4316 4381 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4316..4381") 
COMMENT        #20=(CFSignal (CObList) "intron hEF1a promoter" 15 0 0 582 1520 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "582..1520") 
COMMENT        #21=(CFSignal (CObList) "lac promoter" 30 0 1 5151 5234 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5151..5234)") 
COMMENT        #22=(CFSignal (CObList) "mTRAF2" 4 0 0 1618 3120 0 (CStringList) 
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COMMENT       (CObList) 
COMMENT       (CObList 
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COMMENT        #24=(CFSignal (CObList) "Last modified: 27-JUL-2018" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pEF6-mTRAF2-cMyc'" 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
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COMMENT                          "Created: 07/25/18 09:09" 1) 
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COMMENT                          "Last Modified: 07/27/18 08:51" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7254 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 26-JUL-2018" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "TEL: 0032-9-33-13-843  " 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.genecorner@UGent.be</a>" 
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COMMENT              (LOGPEN 0 0 0) 2.55384e-316 2.5779e-312) 
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FEATURES             Location/Qualifiers
     promoter        1546..1562
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     3181..3198
                     /vntifkey="22"
                     /label=His-tag
     terminator      3227..3451
                     /vntifkey="43"
                     /label=BGH\polyA
     rep_origin      3497..3925
                     /vntifkey="33"
                     /label=f1\ori
     CDS             4382..4780
                     /vntifkey="4"
                     /label=blasticidin\resistance
     terminator      4938..5012
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4938..5012)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(5326..5717)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        3930..4211
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      4168..4243
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             complement(6258..7118)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        386..1529
                     /vntifkey="29"
                     /label=hEF1a\promoter
     misc_marker     3136..3165
                     /vntifkey="22"
                     /label=Myc\epitope
     promoter        4316..4381
                     /vntifkey="30"
                     /label=synthetic\EM7\promoter
     intron          582..1520
                     /vntifkey="15"
                     /label=intron\hEF1a\promoter
     promoter        complement(5151..5234)
                     /vntifkey="30"
                     /label=lac\promoter
     CDS             1618..3120
                     /vntifkey="4"
                     /label=mTRAF2
BASE COUNT     1636 a      1875 c      1952 g      1791 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccgc ggccgctatg 
     1621 gctgcagcca gtgtgacttc ccctggctcc ctagaactgc tacagcctgg cttctccaag 
     1681 accctcctgg ggaccaggtt agaagccaag tacctctgtt cagcctgcaa aaacatcctg 
     1741 cggaggcctt tccaggccca gtgtgggcac cgctactgct ccttctgcct gaccagcatc 
     1801 ctcagctctg ggccccagaa ctgtgctgcc tgtgtctatg aaggcctgta tgaagaaggc 
     1861 atttctattt tagagagtag ttcggccttt ccagataacg ctgcccgcag agaggtggag 
     1921 agcctgccag ctgtctgtcc caatgatgga tgcacttgga aggggacctt gaaagaatac 
     1981 gagagctgcc acgaaggact ttgcccattc ctgctgacgg agtgtcctgc atgtaaaggc 
     2041 ctggtccgcc tcagcgagaa ggagcaccac actgagcagg aatgccccaa aaggagcctg 
     2101 agctgccagc actgcagagc accctgtagc cacgtggacc tggaggtaca ctatgaggtc 
     2161 tgccccaagt ttcccttaac ctgtgatggc tgtggcaaga agaagatccc tcgggagacg 
     2221 tttcaggacc atgttagagc atgcagcaaa tgccgggttc tctgcagatt ccacaccgtt 
     2281 ggctgttcag agatggtgga gactgagaac ctgcaggatc atgagctgca gcggctacgg 
     2341 gaacacctag ccctactgct gagctcattc ttggaggccc aagcctctcc aggaaccttg 
     2401 aaccaggtgg ggccagagct actccagcgg tgccagattt tggagcagaa gatagcaacc 
     2461 tttgagaaca ttgtctgcgt cttgaaccgt gaagtagaga gggtagcagt gactgcagag 
     2521 gcttgtagcc ggcagcaccg gctagaccag gacaagattg aggccctgag taacaaggtg 
     2581 caacagctgg agaggagcat cggcctcaag gacctggcca tggctgacct ggagcagaag 
     2641 gtctccgagt tggaagtatc cacctatgat ggggtcttca tctggaagat ctctgacttc 
     2701 accagaaagc gtcaggaagc cgtagctggc cggacaccag ctatcttctc cccagccttc 
     2761 tacacaagca gatatggcta caagatgtgt ctacgagtct acttgaatgg cgacggcact 
     2821 gggcggggaa ctcatctgtc tctcttcttc gtggtgatga aaggccccaa tgatgctctg 
     2881 ttgcagtggc cttttaatca gaaggtaaca ttgatgttgc tggaccataa caaccgggag 
     2941 catgtgatcg acgcattcag gcccgatgta acctcgtcct ccttccagag gcctgtcagt 
     3001 gacatgaaca tcgccagtgg ctgccccctc ttctgccctg tgtccaagat ggaggccaag 
     3061 aattcctatg tgcgggatga tgcgatcttc atcaaagcta ttgtggacct aacaggactc 
     3121 tctagagggc ccttcgaaca aaaactcatc tcagaagagg atctgaatat gcataccggt 
     3181 catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt 
     3241 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact 
     3301 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat 
     3361 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc 
     3421 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc 
     3481 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 
     3541 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 
     3601 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 
     3661 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 
     3721 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 
     3781 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 
     3841 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 
     3901 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa 
     3961 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 
     4021 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 
     4081 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 
     4141 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 
     4201 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 
     4261 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga 
     4321 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac 
     4381 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat 
     4441 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg 
     4501 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt 
     4561 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg 
     4621 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct 
     4681 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat 
     4741 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcacttcgtg gccgaggagc 
     4801 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 
     4861 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 
     4921 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata 
     4981 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 
     5041 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt 
     5101 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 
     5161 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 
     5221 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 
     5281 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 
     5341 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
     5401 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
     5461 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 
     5521 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 
     5581 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 
     5641 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 
     5701 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 
     5761 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 
     5821 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 
     5881 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 
     5941 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 
     6001 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 
     6061 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 
     6121 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 
     6181 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 
     6241 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 
     6301 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 
     6361 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 
     6421 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 
     6481 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 
     6541 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 
     6601 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 
     6661 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 
     6721 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 
     6781 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 
     6841 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 
     6901 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 
     6961 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 
     7021 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 
     7081 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 
     7141 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 
     7201 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc 
//
