LOCUS       pEGFP-N1-TRAP1          6654 bp    DNA     circular     18-OCT-2012
DEFINITION  LMBP 6239.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6239
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 18-OCT-2012
            
            
            
            
            
COMMENT     VNTDATE|634909693|
COMMENT     VNTNAME|pEGFP-N1-TRAP1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #2=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 942 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 630 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 3323 0 0 "")) 
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FEATURES             Location/Qualifiers
     promoter        1..568
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      complement(3570..4025)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        4177..4458
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      6322..6648
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="E. coli plasmid pMB1 origin"
     terminator      3420..3516
                     /vntifkey="43"
                     /label=SV40\polyA\early
     CDS             2600..3319
                     /vntifkey="4"
                     /label=EGFP
     promoter        4026..4121
                     /vntifkey="30"
                     /label=ampicillin\promoter
     CDS             4550..5344
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
                     /note="Tn5 antibiotic resistance gene"
     terminator      complement(3420..3516)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      4415..4490
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      5535..5604
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     RBS             4122..4156
                     /vntifkey="32"
                     /label=5'\UTR\of\amp,\incl.\RBS
     CDS             641..2578
                     /vntifkey="4"
                     /label=mature\hTRAP1
BASE COUNT     1607 a      1817 c      1802 g      1428 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attcgccgcc atgagcacgc agaccgccga 
      661 ggacaaggag gaacccctgc actcgattat cagcagcaca gagagcgtgc agggttccac 
      721 ttccaaacat gagttccagg ccgagacaaa gaagcttttg gacattgttg cccggtccct 
      781 gtactcagaa aaagaggtgt ttatacggga gctgatctcc aatgccagcg atgccttgga 
      841 aaaactgcgt cacaaactgg tgtctgacgg ccaagcactg ccagaaatgg agattcactt 
      901 gcagaccaat gccgagaaag gcaccatcac catccaggat actggtatcg ggatgacaca 
      961 ggaagagctg gtgtccaacc tggggacgat tgccagatcg gggtcaaagg ccttcctgga 
     1021 tgctctgcag aaccaggctg aggccagcag caagatcatc ggccagtttg gagtgggttt 
     1081 ctactcagct ttcatggtgg ctgacagagt ggaggtctat tcccgctcgg cagccccggg 
     1141 gagcctgggt taccagtggc tttcagatgg ttctggagtg tttgaaatcg ccgaagcttc 
     1201 gggagttaga accgggacaa aaatcatcat ccacctgaaa tccgactgca aggagttttc 
     1261 cagcgaggcc cgggtgcgag atgtggtaac gaagtacagc aacttcgtca gcttcccctt 
     1321 gtacttgaat ggaaggcgga tgaacacctt gcaggccatc tggatgatgg accccaagga 
     1381 tgtcggtgag tggcaacatg aggagttcta ccgctacgtc gcgcaggctc acgacaagcc 
     1441 ccgctacacc ctgcactata agacggacgc accgctcaac atccgcagca tcttctacgt 
     1501 gcccgacatg aaaccgtcca tgtttgatgt gagccgggag ctgggctcca gcgttgcact 
     1561 gtacagccgc aaagtcctca tccagaccaa ggccacggac atcctgccca agtggctgcg 
     1621 cttcatccga ggtgtggtgg acagtgagga cattcccctg aacctcagcc gggagctgct 
     1681 gcaggagagc gcactcatca ggaaactccg ggacgtttta cagcagaggc tgatcaaatt 
     1741 cttcattgac cagagtaaaa aagatgctga gaagtatgca aagttttttg aagattacgg 
     1801 cctgttcatg cgggagggca ttgtgaccgc caccgagcag gaggtcaagg aggacatagc 
     1861 aaagctgctg cgctacgagt cctcggcgct gccctccggg cagctaacca gcctctcaga 
     1921 atacgccagc cgcatgcggg ccggcacccg caacatctac tacctgtgcg cccccaaccg 
     1981 tcacctggca gagcactcac cctactatga ggccatgaag aagaaagaca cagaggttct 
     2041 cttctgcttt gagcagtttg atgagctcac cctgctgcac cttcgtgagt ttgacaagaa 
     2101 gaagctgatc tctgtggaga cggacatagt cgtggatcac tacaaggagg agaagtttga 
     2161 ggacaggtcc ccagccgccg agtgcctatc agagaaggag acggaggagc tcatggcctg 
     2221 gatgagaaat gtgctggggt cgcgtgtcac caacgtgaag gtgaccctcc gactggacac 
     2281 ccaccctgcc atggtcaccg tgctggagat gggggctgcc cgccacttcc tgcgcatgca 
     2341 gcagctggcc aagacccagg aggagcgcgc acagctcctg cagcccacgc tggagatcaa 
     2401 ccccaggcac gcgctcatca agaagctgaa tcagctgcgc gcaagcgagc ctggcctggc 
     2461 tcagctgctg gtggatcaga tatacgagaa cgccatgatt gctgctggac ttgttgacga 
     2521 ccctagggcc atggtgggcc gcttgaatga gctgcttgtc aaggccctgg agcgacaccg 
     2581 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 
     2641 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 
     2701 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 
     2761 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg 
     2821 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt 
     2881 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 
     2941 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 
     3001 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 
     3061 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 
     3121 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 
     3181 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga 
     3241 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 
     3301 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt 
     3361 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa 
     3421 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc 
     3481 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 
     3541 tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt 
     3601 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat 
     3661 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa 
     3721 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg 
     3781 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa 
     3841 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc 
     3901 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 
     3961 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 
     4021 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 
     4081 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 
     4141 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg 
     4201 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 
     4261 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 
     4321 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 
     4381 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 
     4441 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 
     4501 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca 
     4561 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 
     4621 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 
     4681 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 
     4741 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 
     4801 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 
     4861 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 
     4921 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 
     4981 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 
     5041 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 
     5101 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 
     5161 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 
     5221 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 
     5281 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 
     5341 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 
     5401 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 
     5461 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg 
     5521 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca 
     5581 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg 
     5641 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc 
     5701 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc 
     5761 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat 
     5821 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 
     5881 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 
     5941 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 
     6001 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 
     6061 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 
     6121 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 
     6181 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 
     6241 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 
     6301 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 
     6361 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 
     6421 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 
     6481 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 
     6541 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 
     6601 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat 
//
