ID pROSA26-DV3 standard; circular DNA; ; 12047 BP. DE LMBP 6453. CC Accession number: LMBP 6453 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 1-AUG-2013 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS complement(9232..10092) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(10223..10678) FT /vntifkey="33" FT /label=f1\ori FT promoter 10846..10862 FT /vntifkey="30" FT /label=T7\promoter FT rep_origin complement(8289..8691) FT /vntifkey="33" FT /label=pMB1\ori FT promoter complement(8114..8197) FT /vntifkey="30" FT /label=lac\promoter FT promoter complement(8034..8050) FT /vntifkey="30" FT /label=T3\promoter FT terminator 1770..1994 FT /vntifkey="43" FT /label=BGH\polyA FT promoter 6428..6929 FT /vntifkey="29" FT /label=mPGK1\promoter FT terminator 7740..7964 FT /vntifkey="43" FT /label=BGH\polyA FT attenuator 1581..1705 FT /vntifkey="0" FT /label=attR3 FT promoter complement(1521..1573) FT /vntifkey="30" FT /label=lacUV5\promoter FT CDS complement(813..1472) FT /vntifkey="4" FT /label=chloramphenicol\resistance FT CDS complement(166..471) FT /vntifkey="4" FT /label=ccdB FT attenuator complement(1..125) FT /vntifkey="0" FT /label=attR4 FT misc_feature 10884..11964 FT /vntifkey="21" FT /label=5'\UTR\of\ROSA26 FT misc_feature 2155..6386 FT /vntifkey="21" FT /label=3'\UTR\of\ROSA26 SQ Sequence 12047 BP; 3013 A; 2724 C; 2939 G; 3368 t; 3 other; tcaactttgt atagaaaagt tgaacgagaa acgtaaaatg atataaatat caatatatta 60 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca 120 ctatggtcga cctgcagact ggctgtgtat aagggagcct gacatttata ttccccagaa 180 catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc 240 cccgataacg gagaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc 300 cccgatatgc accaccgggt aaagttcacg ggggacttta tctgacagca gacgtgcact 360 ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac 420 aaacagacga taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagc 480 ccctgttctc gtcggcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc 540 cttcctgatt ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg 600 ttgtgcttac cgaaccggag atattgacat catatatgcc ttgagcaact gatagctgtc 660 gctgtcaact gtcactgtaa tacgctgctt catagcatac ctctttttga catacttcgg 720 gtatacatat cagtatatat tcttataccg caaaaatcag cgcgcaaata cgcatactgt 780 tatctggctt ttagtaagcc ggatcctcta gattacgccc cgccctgcca ctcatcgcag 840 tactgttgta attcattaag cattctgccg acatggaagc catcacagac ggcatgatga 900 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg 960 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc 1020 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc 1080 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg 1140 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa 1200 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc 1260 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta 1320 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta 1380 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca 1440 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc 1500 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct 1560 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag 1620 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc 1680 gttcaacttt attatacata gttgataatt cactggccgt cgttttacaa cgtcgtgact 1740 gggaaaacga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 1800 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 1860 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 1920 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 1980 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgacct ctagttggcg 2040 cgccgctaga agatgggcgg gagtcttctg ggcaggctta aaggctaacc tggtgtgtgg 2100 gcgttgtcct gcaggggaat tgaacaggtg taaaattgga gggacaagac ttcccacaga 2160 ttttcggttt tgtcgggaag ttttttaata ggggcaaata aggaaaatgg gaggataggt 2220 agtcatctgg ggttttatgc agcaaaacta caggttatta ttgcttgtga tccgcctcgg 2280 agtattttcc atcgaggtag attaaagaca tgctcacccg agttttatac tctcctgctt 2340 gagatcctta ctacagtatg aaattacagt gtcgcgagtt agactatgta agcagaattt 2400 taatcatttt taaagagccc agtacttcat atccatttct cccgctcctt ctgcagcctt 2460 atcaaaaggt attttagaac actcatttta gccccatttt catttattat actggcttat 2520 ccaaccccta gacagagcat tggcattttc cctttcctga tcttagaagt ctgatgactc 2580 atgaaaccag acagattagt tacatacacc acaaatcgag gctgtagctg gggcctcaac 2640 actgcagttc ttttataact ccttagtaca ctttttgttg atcctttgcc ttgatcctta 2700 attttcagtg tctatcacct ctcccgtcag gtggtgttcc acatttgggc ctattctcag 2760 tccagggagt tttacaacaa tagatgtatt gagaatccaa cctaaagctt aactttccac 2820 tcccatgaat gcctctctcc tttttctcca tttataaact gagctattaa ccattaatgg 2880 tttccaggtg gatgtctcct cccccaatat tacctgatgt atcttacata ttgccaggct 2940 gatattttaa gacattaaaa ggtatatttc attattgagc cacatggtat tgattactgc 3000 ttactaaaat tttgtcattg tacacatctg taaaaggtgg ttccttttgg aatgcaaagt 3060 tcaggtgttt gttgtctttc ctgacctaag gtcttgtgag cttgtatttt ttctatttaa 3120 gcagtgcttt ctcttggact ggcttgactc atggcattct acacgttatt gctggtctaa 3180 atgtgatttt gccaagcttc ttcaggacct ataattttgc ttgacttgta gccaaacaca 3240 agtaaaatga ttaagcaaca aatgtatttg tgaagcttgg tttttaggtt gttgtgttgt 3300 gtgtgcttgt gctctataat aatactatcc aggggctgga gaggtggctc ggagttcaag 3360 agcacagact gctcttccag aagtcctgag ttcaattccc agcaaccaca tggtggctca 3420 caaccatctg taatgggatc tgatgccctc ttctggtgtg tctgaagacc acaagtgtat 3480 tcacattaaa taaataaatc ctccttcttc ttcttttttt tttttttaaa gagaatactg 3540 tctccagtag aatttactga agtaatgaaa tactttgtgt ttgttccaat atggtagcca 3600 ataatcaaat tactctttaa gcactggaaa tgttaccaag gaactaattt ttatttgaag 3660 tgtaactgtg gacagaggag ccataactgc agacttgtgg gatacagaag accaatgcag 3720 actttaatgt cttttctctt acactaagca ataaagaaat aaaaattgaa cttctagtat 3780 cctatttgtt aaactgctag ctttacttaa cttttgtgct tcatctatac aaagctgaaa 3840 gctaagtctg cagccattac taaacatgaa agcaagtaat gataattttg gatttcaaaa 3900 atgtagggcc agagtttagc cagccagtgg tggtgcttgc ctttatgcct ttaatcccag 3960 cactctggag gcagagacag gcagatctct gagtttgagc ccagcctggt ctacacatca 4020 agttctatct aggatagcca ggaatacaca cagaaaccct gttggggagg ggggctctga 4080 gatttcataa aattataatt gaagcattcc ctaatgagcc actatggatg tggctaaatc 4140 cgtctacctt tctgatgaga tttgggtatt attttttctg tctctgctgt tggttgggtc 4200 ttttgacact gtgggctttc tttaaagcct ccttccctgc catgtggtct cttgtttgct 4260 actaacttcc catggcttaa atggcatggc tttttgcctt ctaagggcag ctgctgagat 4320 ttgcagcctg atttccaggg tggggttggg aaatctttca aacactaaaa ttgtccttta 4380 attttttttt taaaaaatgg gttatataat aaacctcata aaatagttat gaggagtgag 4440 gtggactaat attaaatgag tccctcccct ataaaagagc tattaaggct ttttgtctta 4500 tacttaactt tttttttaaa tgtggtatct ttagaaccaa gggtcttaga gttttagtat 4560 acagaaactg ttgcatcgct taatcagatt ttctagtttc aaatccagag aatccaaatt 4620 cttcacagcc aaagtcaaat taagaatttc tgacttttaa tgttaatttg cttactgtga 4680 atataaaaat gatagctttt cctgaggcag ggtctcacta tgtatctctg cctgatctgc 4740 aacaagatat gtagactaaa gttctgcctg cttttgtctc ctgaatacta aggttaaaat 4800 gtagtaatac ttttggaact tgcaggtcag attcttttat aggggacaca ctaagggagc 4860 ttgggtgata gttggtaaaa tgtgtttcaa gtgatgaaaa cttgaattat tatcaccgca 4920 acctactttt taaaaaaaaa agccaggcct gttagagcat gcttaaggga tccctaggac 4980 ttgctgagca cacaagagta gttacttggc aggctcctgg tgagagcata tttcaaaaaa 5040 caaggcagac aaccaagaaa ctacagttaa ggttacctgt ctttaaacca tctgcatata 5100 cacagggata ttaaaatatt ccaaataata tttcattcaa gttttccccc atcaaattgg 5160 gacatggatt tctccggtga ataggcagag ttggaaacta aacaaatgtt ggttttgtga 5220 tttgtgaaat tgttttcaag tgatagttaa agcccatgag atacagaaca aagctgctat 5280 ttcgaggtct cttggtttat actcagaagc acttctttgg gtttccctgc actatcctga 5340 tcatgtgcta ggcctacctt aggctgattg ttgttcaaat aaacttaagt ttcctgtcag 5400 gtgatgtcat atgatttcat atatcaaggc aaaacatgtt atatatgtta aacatttgta 5460 cttaatgtga aagttaggtc tttgtgggtt ttgattttta attttcaaaa cctgagctaa 5520 ataagtcatt tttacatgtc ttacatttgg tggaattgta taattgtggt ttgcaggcaa 5580 gactctctga cctagtaacc ctacctatag agcactttgc tgggtcacaa gtctaggagt 5640 caagcatttc accttgaagt tgagacgttt tgttagtgta tactagttta tatgttggag 5700 gacatgttta tccagaagat attcaggact atttttgact gggctaagga attgattctg 5760 attagcactg ttagtgagca ttgagtggcc tttaggcttg aattggagtc acttgtatat 5820 ctcaaataat gctggccttt tttaaaaagc ccttgttctt tatcaccctg ttttctacat 5880 aatttttgtt caaagaaata cttgtttgga tctccttttg acaacaatag catgttttca 5940 agccatattt tttttccttt tttttttttt ttttggtttt tcgagacagg gtttctctgt 6000 atagccctgg ctgtcctgga actcactttg tagaccaggc tggcctcgaa ctcagaaatc 6060 cgcctgcctc tgcctcctga gtgccgggat taaaggcgtg caccaccacg cctggctaag 6120 ttggatattt tgtatataac tataaccaat actaactcca ctgggtggat ttttaattca 6180 gtcagtagtc ttaagtggtc tttattggcc cttcattaaa atctactgtt cactctaaca 6240 gaggctgttg gtactagtgg cacttaagca acttcctacg gatatactag cagattaagg 6300 gtcagggata gaaactagtc tagcgttttg tatacctacc agctttatac taccttgttc 6360 tgatagaaat atttcaggac atctagctta tcgatccgtc gacggtatcg ataagcttga 6420 tatcgaattc taccgggtag gggaggcgct tttccaaggc agtctgagca tgcgcttagc 6480 agccccgctg gcacttggcg ctacacaagt ggcctytggc ctcgcacaca ttccacatcc 6540 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctwctcct 6600 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtsaggacg tgacaaatgg 6660 aagtagcacg tctcactagt ctcgtcagat ggacagcacc gctgagcaat ggaagcgggt 6720 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 6780 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 6840 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 6900 ctcctcttcc tcatctccgg gcctttcgac ctgcaggtcc tcgccatgga tcctgatgat 6960 gttgttattc ttctaatctt ttgtatggaa aacttttctt cgtaccacgg gactaaacct 7020 ggttatgtag attccattca aaaaggtata caaaagccaa aatctggtac acaaggaaat 7080 tatgacgatg attggaaagg gttttatagt accgacaata aatacgacgc tgcgggatac 7140 tctgtagata atgaaaaccc gctctctgga aaagctggag gcgtggtcaa agtgacgtat 7200 ccaggactga cgaaggttct cgcactaaaa gtggataatg ccgaaactat taagaaagag 7260 ttaggtttaa gtctcactga accgttgatg gagcaagtcg gaacggaaga gtttatcaaa 7320 aggttcggtg atggtgcttc gcgtgtagtg ctcagccttc ccttcgctga ggggagttct 7380 agcgttgaat atattaataa ctgggaacag gcgaaagcgt taagcgtaga acttgagatt 7440 aattttgaaa cccgtggaaa acgtggccaa gatgcgatgt atgagtatat ggctcaagcc 7500 tgtgcaggaa atcgtgtcag gcgatctctt tgtgaaggaa ccttacttct gtggtgtgac 7560 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag 7620 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg 7680 aactgatgaa tgggagcagt ggtggaatgc agatcctaga gctcgctgat cagcctcgac 7740 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct 7800 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct 7860 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg 7920 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag 7980 aaccagctgg ggctcgacct cgaggggggg cccggtaccc agcttttgtt ccctttagtg 8040 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 8100 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 8160 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 8220 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 8280 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 8340 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 8400 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 8460 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 8520 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 8580 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 8640 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 8700 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 8760 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 8820 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 8880 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 8940 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 9000 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 9060 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 9120 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 9180 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 9240 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 9300 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 9360 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 9420 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 9480 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 9540 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 9600 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 9660 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 9720 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 9780 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 9840 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 9900 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 9960 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 10020 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 10080 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 10140 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 10200 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta 10260 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 10320 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca 10380 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 10440 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta 10500 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 10560 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg 10620 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc 10680 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta 10740 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg 10800 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta 10860 tagggcgaat tggagctccc cgcggcaggc cctccgagcg tggtggagcc gttctgtgag 10920 acagccgggt acgagtcgtg acgctggaag gggcaagcgg gtggtgggca ggaatgcggt 10980 ccgccctgca gcaaccggag ggggagggag aagggagcgg aaaagtctcc accggacgcg 11040 gccatggctc gggggggggg gggcagcgga ggagcgcttc cggccgacgt ctcgtcgctg 11100 attggcttct tttcctcccg ccgtgtgtga aaacacaaat ggcgtgtttt ggttggcgta 11160 aggcgcctgt cagttaacgg cagccggagt gcgcagccgc cggcagcctc gctctgccca 11220 ctgggtgggg cgggaggtag gtggggtgag gcgagctgga cgtgcgggcg cggtcggcct 11280 ctggcggggc gggggagggg agggagggtc agcgaaagta gctcgcgcgc gagcggccgc 11340 ccaccctccc cttcctctgg gggagtcgtt ttacccgccg ccggccgggc ctcgtcgtct 11400 gattggctct cggggcccag aaaactggcc cttgccattg gctcgtgttc gtgcaagttg 11460 agtccatccg ccggccagcg ggggcggcga ggaggcgctc ccaggttccg gccctcccct 11520 cggccccgcg ccgcagagtc tggccgcgcg cccctgcgca acgtggcagg aagcgcgcgc 11580 tgggggcggg gacgggcagt agggctgagc ggctgcgggg cgggtgcaag cacgtttccg 11640 acttgagttg cctcaagagg ggcgtgctga gccagacctc catcgcgcac tccggggagt 11700 ggagggaagg agcgagggct cagttgggct gttttggagg caggaagcac ttgctctccc 11760 aaagtcgctc tgagttgtta tcagtaaggg agctgcagtg gagtaggcgg ggagaaggcc 11820 gcacccttct ccggaggggg gaggggagtg ttgcaatacc tttctgggag ttctctgctg 11880 cctcctggct tctgaggacc gccctgggcc tgggagaatc ccttgccccc tcttcccctc 11940 gtgatctgca actccagtct ttcttaatct agcttatcga taccgtcgac ccgagcggat 12000 aacaatttca cacaggaaac agctatgacc atgattacgc caagcta 12047 //