LOCUS       pMSCV-His-AU-Ub-K63        6588 bp    DNA     circular     17-DEC-2009
DEFINITION  LMBP 6594.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|537805669|
COMMENT     VNTNAME|pMSCV-His-AU-Ub-K63|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

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COMMENT                                    #108=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #109=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   1622..1624" 1))) "R48") 
COMMENT                            #110=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 1667 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #111=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 1667  End: 1669" 1) 
COMMENT                                    #112=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #113=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   1667..1669" 1))) "K63") 
COMMENT                            #114=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 4794 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #115=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 4794  End: 5654" 1) 
COMMENT                                    #116=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #117=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   4794..5654" 1))) "Amp (r)"))) 
COMMENT                          "Misc. Feature (10 total)") 
COMMENT                     #118=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #119=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2251 (3 #25# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2251  End: 2847" 1))) "PuroR"))) 
COMMENT                           "Misc. Marker (1 total)") 
COMMENT                     #121=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 85 (7 85 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #122=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 662 (3 #24# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 662   End: 1405" 1))) 
COMMENT                                   "Packaging"))) "Misc. Difference (1 total)") 
COMMENT                     #124=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 104 (7 104 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #125=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4347 (3 #23# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #126=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4347  End: 4990" 1))) 
COMMENT                                   "pUC-ori"))) "oriT (1 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #127=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #128=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 24734336 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #129=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #130=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1717 " 1))))) "ApaI: 1 site") 
COMMENT                            5 1 "GGGCCC" "CCCGGG") 
COMMENT                      #131=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 24791264 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #132=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #133=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1416 " 1))))) 
COMMENT                             "HindIII: 1 site") 1 5 "AAGCTT" "TTCGAA"))) 
COMMENT                    "Restriction/Methylation Map") 
COMMENT              #134=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 15 (12 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #135=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 0 (13 0 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #136=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 1 (14 1 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #137=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24945464 (4 #30# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #138=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #139=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1069 - 1233" 
COMMENT                                                    1))) "1069(d1) (55 codons)") 
COMMENT                                      #140=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24733488 (4 #39# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #141=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #142=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2251 - 2847" 
COMMENT                                                    1))) "2251(d1) (199 codons)") 
COMMENT                                      #143=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24882792 (4 #40# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #144=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #145=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4288 - 4539" 
COMMENT                                                    1))) "4288(d1) (84 codons)") 
COMMENT                                      #146=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24736440 (4 #41# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #147=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #148=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4924 - 5187" 
COMMENT                                                    1))) "4924(d1) (88 codons)") 
COMMENT                                      #149=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 33961256 (4 #42# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #150=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #151=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  5197 - 5364" 
COMMENT                                                    1))) "5197(d1) (56 codons)") 
COMMENT                                      #152=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24105664 (4 #52# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #153=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #154=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  6031 - 6333" 
COMMENT                                                    1))) "6031(d1) (101 codons)"))) 
COMMENT                                    "Phase #1 (6 frames)") 
COMMENT                              #155=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 2 (14 2 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #156=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24924848 (4 #32# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #157=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #158=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1424 - 1708" 
COMMENT                                                    1))) "1424(d2) (95 codons)") 
COMMENT                                      #159=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 34065328 (4 #44# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #160=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #161=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2087 - 2251" 
COMMENT                                                    1))) "2087(d2) (55 codons)") 
COMMENT                                      #162=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 25047192 (4 #50# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #163=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #164=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  5867 - 6025" 
COMMENT                                                    1))) "5867(d2) (53 codons)") 
COMMENT                                      #165=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 33843256 (4 #51# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #166=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #167=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  6260 - 6463" 
COMMENT                                                    1))) "6260(d2) (68 codons)"))) 
COMMENT                                    "Phase #2 (4 frames)") 
COMMENT                              #168=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 3 (14 3 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #169=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 34035200 (4 #29# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #170=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #171=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  798 - 983" 1))) 
COMMENT                                            "798(d3) (62 codons)") 
COMMENT                                      #172=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24688736 (4 #33# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #173=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #174=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1233 - 1493" 
COMMENT                                                    1))) "1233(d3) (87 codons)") 
COMMENT                                      #175=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24904608 (4 #35# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #176=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #177=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1635 - 1841" 
COMMENT                                                    1))) "1635(d3) (69 codons)") 
COMMENT                                      #178=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24104896 (4 #36# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #179=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #180=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2007 - 2927" 
COMMENT                                                    1))) "2007(d3) (307 codons)") 
COMMENT                                      #181=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24853928 (4 #49# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #182=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #183=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  6063 - 6233" 
COMMENT                                                    1))) "6063(d3) (57 codons)"))) 
COMMENT                                    "Phase #3 (5 frames)"))) "Direct strand") 
COMMENT                      #184=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (13 1 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #185=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 1 (14 -1 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #186=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24740112 (4 #37# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #187=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #188=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1471 - 1644" 
COMMENT                                                    1))) "1471(c1) (58 codons)") 
COMMENT                                      #189=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 33895264 (4 #38# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #190=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #191=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2212 - 2901" 
COMMENT                                                    1))) "2212(c1) (230 codons)"))) 
COMMENT                                    "Phase #1 (2 frames)") 
COMMENT                              #192=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 2 (14 -2 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #193=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24903040 (4 #31# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #194=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #195=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  342 - 527" 1))) 
COMMENT                                            "342(c2) (62 codons)") 
COMMENT                                      #196=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 33985392 (4 #43# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #197=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #198=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1800 - 1997" 
COMMENT                                                    1))) "1800(c2) (66 codons)") 
COMMENT                                      #199=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 34444800 (4 #45# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #200=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #201=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2340 - 2630" 
COMMENT                                                    1))) "2340(c2) (97 codons)") 
COMMENT                                      #202=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24815376 (4 #46# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #203=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #204=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2763 - 2963" 
COMMENT                                                    1))) "2763(c2) (67 codons)") 
COMMENT                                      #205=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24759464 (4 #47# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #206=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #207=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  3054 - 3305" 
COMMENT                                                    1))) "3054(c2) (84 codons)") 
COMMENT                                      #208=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24832064 (4 #48# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #209=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #210=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4797 - 5654" 
COMMENT                                                    1))) "4797(c2) (286 codons)"))) 
COMMENT                                    "Phase #2 (6 frames)") 
COMMENT                              #211=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 3 (14 -3 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #212=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24103144 (4 #26# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #213=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #214=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  104 - 355" 1))) 
COMMENT                                            "104(c3) (84 codons)") 
COMMENT                                      #215=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 33876880 (4 #27# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #216=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #217=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  365 - 637" 1))) 
COMMENT                                            "365(c3) (91 codons)") 
COMMENT                                      #218=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24943840 (4 #28# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #219=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #220=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  677 - 934" 1))) 
COMMENT                                            "677(c3) (86 codons)") 
COMMENT                                      #221=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 33876776 (4 #34# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #222=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #223=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1376 - 1885" 
COMMENT                                                    1))) "1376(c3) (170 codons)") 
COMMENT                                      #224=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 24913032 (4 #53# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #225=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #226=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  6107 - 6427" 
COMMENT                                                    1))) "6107(c3) (107 codons)"))) 
COMMENT                                    "Phase #3 (5 frames)"))) 
COMMENT                            "Complementary strand"))) "Open Reading Frames")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #227=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #228=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #229=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
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FEATURES             Location/Qualifiers
     misc_feature    4794..5654
                     /vntifkey="21"
                     /label=Amp\(r)
     misc_feature    1424..1450
                     /vntifkey="21"
                     /label=His-tag
     misc_feature    1451..1480
                     /vntifkey="21"
                     /label=AU1-tag
     CDS             1481..1711
                     /vntifkey="4"
                     /label=hUb-K63
     misc_feature    1496..1498
                     /vntifkey="21"
                     /label=R6
     misc_feature    1511..1513
                     /vntifkey="21"
                     /label=R11
     misc_feature    1559..1561
                     /vntifkey="21"
                     /label=R27
     misc_feature    1565..1567
                     /vntifkey="21"
                     /label=R29
     misc_feature    1577..1579
                     /vntifkey="21"
                     /label=R33
     misc_feature    1622..1624
                     /vntifkey="21"
                     /label=R48
     misc_feature    1667..1669
                     /vntifkey="21"
                     /label=K63
     oriT            4347..4990
                     /vntifkey="104"
                     /label=pUC-ori
     misc_difference 662..1405
                     /vntifkey="85"
                     /label=Packaging
     misc_marker     2251..2847
                     /vntifkey="22"
                     /label=PuroR
BASE COUNT     1490 a      1880 c      1749 g      1469 t 
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 
     1381 ccctcactcc ttctctaggc gccggaatta gatcaagctt aaaatggtac atcaccatca 
     1441 ccatcaccat gccatggaca cataccgcta catcggatcc atgcagatct tcgtgaggac 
     1501 cctgaccggc aggaccatca ctctggaggt ggagcccagt gacaccatcg aaaatgtgag 
     1561 ggccagaatc caagataggg aaggcatccc ccccgaccag cagaggctca tctttgcagg 
     1621 caggcagctg gaagatggcc gcactctttc tgactacaac atccagaaag agtcgaccct 
     1681 gcacctggtc ctgcgtctca gaggtgggta ggggcccaat tctaccgggt aggggaggcg 
     1741 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac 
     1801 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg 
     1861 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc 
     1921 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc 
     1981 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa 
     2041 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc 
     2101 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca 
     2161 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc 
     2221 ctttcgacct gcagcccaag ctagcttacc atgaccgagt acaagcccac ggtgcgcctc 
     2281 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 
     2341 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 
     2401 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 
     2461 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 
     2521 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 
     2581 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 
     2641 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 
     2701 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 
     2761 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 
     2821 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc 
     2881 gaaaggagcg cacgacccca tgcatcgata aaataaaaga ttttatttag tctccagaaa 
     2941 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt 
     3001 tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag 
     3061 agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc 
     3121 agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca 
     3181 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac 
     3241 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag 
     3301 cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt 
     3361 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg 
     3421 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatgggtaa cagtttcttg 
     3481 aagttggaga acaacattct gagggtagga gtcgaatatt aagtaatcct gactcaatta 
     3541 gccactgttt tgaatccaca tactccaata ctcctgaaat agttcattat ggacagcgca 
     3601 gaagagctgg ggagaattaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 
     3661 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 
     3721 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 
     3781 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 
     3841 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 
     3901 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 
     3961 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 
     4021 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 
     4081 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 
     4141 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 
     4201 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 
     4261 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 
     4321 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 
     4381 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 
     4441 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 
     4501 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 
     4561 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 
     4621 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 
     4681 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 
     4741 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 
     4801 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 
     4861 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 
     4921 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 
     4981 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 
     5041 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 
     5101 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 
     5161 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 
     5221 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 
     5281 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 
     5341 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 
     5401 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 
     5461 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 
     5521 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 
     5581 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 
     5641 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 
     5701 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 
     5761 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 
     5821 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 
     5881 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 
     5941 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 
     6001 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 
     6061 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 
     6121 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 
     6181 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 
     6241 acggcgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc 
     6301 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt 
     6361 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc 
     6421 cggccacgat gcgtccggcg tagaggcgat tagtccaatt tgttaaagac aggatatcag 
     6481 tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc 
     6541 catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaa 
//
