LOCUS       pB2H-delta-omega        8342 bp    DNA     circular     12-JUL-2010
DEFINITION  LMBP 6701.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6701
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 12-JUL-2010
            
            
            
COMMENT     VNTDATE|559585403|
COMMENT     VNTNAME|pB2H-delta-omega|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pB2H-delta-omega" 0 8342 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 5 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 150 0 
COMMENT        1 0 (CHomObj 1 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) (CWordArray) 
COMMENT        (CStringList "BamHI" "HincII" "HindIII" "NcoI" "SacI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "lacI (1)" 
COMMENT             "GTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTCAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATA" 
COMMENT             "Fragment of E. coli lactose operon J01636; Length: 1000" 0 
COMMENT             (CStringList) 0) 
COMMENT         #1=(COligo "lacI (2)" 
COMMENT             "CGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGA" 
COMMENT             "Fragment of E. coli lactose operon J01636; Length: 83" 0 
COMMENT             (CStringList) 0)) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 
COMMENT        "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 1 0 0 0 0 1 8342 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 2449 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HincII" "gtyrac" 4 0 4 1377 0 2001 0 2571 0 
COMMENT            7083 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2590 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 70 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "SacI" "gagctc" 6 0 2 491 0 2465 0 0 "")) 
COMMENT       (CObList 
COMMENT        #7=(CFSignal (CObList) "pMB1 ori" 33 0 0 5446 5848 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5446..5848") 
COMMENT        #8=(CFSignal (CObList) "rop" 4 0 1 6094 6285 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6094..6285)") 
COMMENT        #9=(CFSignal (CObList) "T7 promoter" 30 0 0 8326 8342 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "8326..8342") 
COMMENT        #10=(CFSignal (CObList) "tac promoter" 30 0 1 2539 2573 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2539..2573)") 
COMMENT        #11=(CFSignal (CObList) "SP6 promoter" 30 0 1 2603 2619 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2603..2619)") 
COMMENT        #12=(CFSignal (CObList) "lac promoter" 30 0 1 2680 2763 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2680..2763)") 
COMMENT        #13=(CFSignal (CObList) "T7 terminator" 43 0 0 3388 3435 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3388..3435") 
COMMENT        #14=(CFSignal (CObList) "f1 ori" 33 0 0 3472 3927 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3472..3927") 
COMMENT        #15=(CFSignal (CObList) "ampicillin resistance" 4 0 1 4042 4902 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4042..4902)") 
COMMENT        #16=(CFSignal (CObList) "lacZ delta-omega fragment" 4 0 1 78 2441 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(78..2441)") 
COMMENT        #17=(CFSignal (CObList) "5' UTR of lacZ gene, incl. RBS" 32 0 1 2503 
COMMENT             2538 0 (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "lacI" 4 0 1 6857 7939 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6857..7939)") 
COMMENT        #19=(CFSignal (CObList) "lacI promoter" 30 0 1 7940 8017 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7940..8017)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #20=(CFSignal (CObList) "LMBP 6701" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #21=(CFSignal (CObList) "Last modified: 12-JUL-2010" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #22=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #23=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pB2H-delta-omega" 
COMMENT                    2) 5 "" 0 4) 
COMMENT              #24=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pB2H-delta-omega'" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 6701." 
COMMENT                          1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: De Smet Lina" 
COMMENT                          1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 07/12/10 04:23" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 07/12/10 04:23" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8342 bp" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #33=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 22-JUN-2010" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #36=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "De Smet Lina" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:lina.desmet@ugent.be\">lina.desmet@ugent.be</a>" 
COMMENT                          1))) "Original Author") 
COMMENT              #39=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #40=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #41=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #42=(CLinePar (CParagraph 0 (15 #20# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 6701") 
COMMENT                     #43=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #44=(CLinePar (CParagraph 0 (15 #21# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 12-JUL-2010"))) 
COMMENT                   "Annotations") 
COMMENT              #45=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #46=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #47=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 78 (3 #16# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 78    End: 2441 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(78..2441)" 1))) 
COMMENT                                 "lacZ delta-omega fragment") 
COMMENT                            #51=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4042 (3 #15# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4042  End: 4902 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4042..4902)" 1))) 
COMMENT                                 "ampicillin resistance") 
COMMENT                            #55=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6094 (3 #8# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6094  End: 6285 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(6094..6285)" 1))) "rop") 
COMMENT                            #59=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6857 (3 #18# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6857  End: 7939 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(6857..7939)" 1))) "lacI"))) 
COMMENT                          "CDS (4 total)") 
COMMENT                     #63=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #64=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2539 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2539  End: 2573 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2539..2573)" 1))) 
COMMENT                                 "tac promoter") 
COMMENT                            #68=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2603 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2603  End: 2619 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2603..2619)" 1))) 
COMMENT                                 "SP6 promoter") 
COMMENT                            #72=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2680 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2680  End: 2763 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2680..2763)" 1))) 
COMMENT                                 "lac promoter") 
COMMENT                            #76=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7940 (3 #19# 0) 1 2 2 1 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7940  End: 8017 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "repressor lacI promoter, incl. RBS" 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(7940..8017)" 1))) 
COMMENT                                 "lacI promoter") 
COMMENT                            #81=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 8326 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 8326  End: 8342" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   8326..8342" 1))) "T7 promoter"))) 
COMMENT                          "Promoter Prokaryotic (5 total)") 
COMMENT                     #85=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 32 (7 32 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #86=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2503 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2503  End: 2538 (Complementary)" 
COMMENT                                        1))) "5' UTR of lacZ gene, incl. RBS"))) 
COMMENT                          "RBS (1 total)") 
COMMENT                     #88=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #89=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3472 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3472  End: 3927" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3472..3927" 1))) "f1 ori") 
COMMENT                            #93=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5446 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5446  End: 5848" 1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5446..5848" 1))) "pMB1 ori"))) 
COMMENT                          "Replication Origin (2 total)") 
COMMENT                     #97=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #98=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3388 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3388  End: 3435" 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   3388..3435" 1))) "T7 terminator"))) 
COMMENT                          "Terminator (1 total)"))) "Feature Map") 
COMMENT              #102=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #103=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #104=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #105=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2449 " 1))))) "BamHI: 1 site") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
COMMENT                      #106=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #107=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #108=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2590 " 1))))) 
COMMENT                             "HindIII: 1 site") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #109=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #110=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #111=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 70 " 1))))) "NcoI: 1 site") 1 
COMMENT                            5 "CCATGG" "GGTACC") 
COMMENT                      #112=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #113=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #114=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 491 " 1) 
COMMENT                                      #115=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2465 " 1))))) "SacI: 2 sites") 
COMMENT                            5 1 "GAGCTC" "CTCGAG") 
COMMENT                      #116=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #117=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #118=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1377 " 1) 
COMMENT                                      #119=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2001 " 1) 
COMMENT                                      #120=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 2571 " 1) 
COMMENT                                      #121=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 7083 " 1))))) 
COMMENT                             "HincII: 4 sites") 3 3 "GTYRAC" "CARYTG"))) 
COMMENT                    "Restriction/Methylation Map:  (no less than 1 site) and (no more than 5 sites)")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #122=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #123=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #124=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #125=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #126=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #127=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #128=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) 
COMMENT          #129=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #130=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #131=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #132=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #133=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #134=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #135=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #136=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #137=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #138=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #139=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #140=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) #141=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #142=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #143=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #144=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #145=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #146=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #147=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #148=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #149=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #150=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #151=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #152=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #153=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #154=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #155=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #156=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #157=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #158=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #159=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #160=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 16711680 
COMMENT                1 5 "@N (@S)" 0) 
COMMENT          #161=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #162=(CWidgetStyle "~Scrap" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #163=(CWidgetStyle "Label 0" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711935 1 5 "@N (@S)" 0) 
COMMENT          #164=(CWidgetStyle "Label 1" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #165=(CWidgetStyle "Label 5" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #166=(CWidgetStyle "Intron Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #167=(CWidgetStyle "Misc_Feature Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #168=(CWidgetStyle "Promoter_E Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #169=(CWidgetStyle "Promoter_P Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #170=(CWidgetStyle "Rep_Origin Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #171=(CWidgetStyle "Terminator Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #172=(CWidgetStyle "oriT Label" 1 (LOGPEN 0 0 13107) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #173=(CWidgetStyle "Label 6" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #174=(CWidgetStyle "Intron" 1 (LOGPEN 0 0 16711680) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #175=(CWidgetStyle "Label 7" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #176=(CWidgetStyle "Label 8" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #177=(CWidgetStyle "Label 9" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #178=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #179=(CWidgetStyle "Label 11" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #180=(CWidgetStyle "Label 12" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #181=(CWidgetStyle "Uniq RSite" 1 (LOGPEN 0 0 16750899) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #182=(CWidgetStyle "Label 13" 1 (LOGPEN 0 0 16763904) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #183=(CWidgetStyle "Label 14" 1 (LOGPEN 0 0 39168) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #184=(CWidgetStyle "Label 15" 1 (LOGPEN 0 0 16750848) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #185=(CWidgetStyle "Marker" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #186=(CWidgetStyle "Marker Label" 1 (LOGPEN 0 0 0) 3 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #187=(CWidgetStyle "Shape 6" 1 (LOGPEN 0 0 0) 3 1 (LOGBRUSH 0 128 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #188=(CWidgetStyle "Shape 7" 1 (LOGPEN 0 0 16711680) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #189=(CWidgetStyle "Shape 8" 1 (LOGPEN 0 0 3381504) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #190=(CWidgetStyle "Label 16" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #191=(CWidgetStyle "Shape 9" 1 (LOGPEN 0 0 16737792) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #192=(CWidgetStyle "Label 17" 1 (LOGPEN 0 0 16737792) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #193=(CWidgetStyle "Label 18" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #194=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #195=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #196=(CWidgetStyle "Label 19" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #197=(CWidgetStyle "Shape 11" 1 (LOGPEN 0 0 153) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #198=(CWidgetStyle "Shape 12" 1 (LOGPEN 0 0 10040064) 4 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #199=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #200=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #201=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #202=(CWidgetStyle "RBS Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #203=(CWidgetStyle "Label 22" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #204=(CWidgetStyle "Label 23" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #205=(CWidgetStyle "Shape 14" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #206=(CWidgetStyle "Attenuator" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #207=(CWidgetStyle "Shape 15" 1 (LOGPEN 0 0 6684774) 4 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #208=(CWidgetStyle "Promoter_P" 1 (LOGPEN 0 0 6684774) 8 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #209=(CWidgetStyle "Shape 16" 1 (LOGPEN 0 0 6684774) 7 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #210=(CWidgetStyle "Shape 17" 1 (LOGPEN 0 0 0) 7 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #211=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #212=(CWidgetStyle "Label 24" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #213=(CWidgetStyle "Label 25" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #214=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #215=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #216=(CWidgetStyle "Label 27" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #217=(CWidgetStyle "Shape 19" 1 (LOGPEN 0 0 16763955) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #218=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #219=(CWidgetStyle "Shape 21" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16763955 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #220=(CWidgetStyle "Attenuator Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #221=(CWidgetStyle "5'UTR Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #222=(CWidgetStyle "3'UTR Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #223=(CWidgetStyle "Label 28" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #224=(CWidgetStyle "Label 29" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #225=(CWidgetStyle "Mutation Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #226=(CWidgetStyle "tRNA Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #227=(CWidgetStyle "Label 30" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #228=(CWidgetStyle "Label 31" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #229=(CWidgetStyle "Label 32" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 16711680 
COMMENT                1 5 "@N (@S)" 0) 
COMMENT          #230=(CWidgetStyle "MSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #231=(CWidgetStyle "Label 33" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #232=(CWidgetStyle "Label 34" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #233=(CWidgetStyle "Shape 30" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #234=(CWidgetStyle "Shape 31" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #235=(CWidgetStyle "Label 35" 1 (LOGPEN 0 0 3394560) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #236=(CWidgetStyle "Label 36" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #237=(CWidgetStyle "Label 37" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 8421376 1 5 "@N (@S)" 0) 
COMMENT          #238=(CWidgetStyle "Label 38" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #239=(CWidgetStyle "Label 39" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 65280 1 5 "@N (@S)" 0) 
COMMENT          #240=(CWidgetStyle "Label 40" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 8388736 1 5 "@N (@S)" 0) 
COMMENT          #241=(CWidgetStyle "Label 41" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711935 1 5 "@N (@S)" 0) 
COMMENT          #242=(CWidgetStyle "Label 42" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16776960 1 5 "@N (@S)" 0) 
COMMENT          #243=(CWidgetStyle "Label 43" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 128 1 5 "@N (@S)" 0) 
COMMENT          #244=(CWidgetStyle "Label 44" 1 (LOGPEN 0 0 39321) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #245=(CWidgetStyle "Label 45" 1 (LOGPEN 0 0 3381657) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #246=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 39321) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #247=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 16724736) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 20" 1 "Label 21") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 21") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 30") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 
COMMENT         -8342) 1 1 0 1 1 
COMMENT        (mapper: 18.3634 -18.267 40.3368 40.3368 0.01 10 -1 8342 8342 1 0 0) 
COMMENT        #248=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -464 100) 
COMMENT              (CObjectList 
COMMENT               #249=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1097 100) 
COMMENT                     (CObjectList 
COMMENT                      #250=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #128# 35417188 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27494 
COMMENT                             6.27294 1 0.0214037) 0.0752) 
COMMENT                      #251=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #125# 2081718712 100) 
COMMENT                            (LOGPEN 0 0 16724787) 1 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "pB2H-delta-omega" "@N" 1 0.0495825 
COMMENT                            -0.814641 0 -10 11.4288 1.56185 Nil) 
COMMENT                      #252=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #124# 2081718712 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 73 27 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "8342 bp" "@L bp" 16 
COMMENT                            0.0743738 -2.60655 0 -10.8 4.48722 1.80976 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #253=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -675 100) 
COMMENT                     (CObjectList 
COMMENT                      #254=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -816 100) 
COMMENT                            (CObjectList 
COMMENT                             #255=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #126# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    1.54686 1.69125 1 0.082322) 0.8 1.8 1) 
COMMENT                             #256=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #200# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "rop" "@N" 1 
COMMENT                                   -13.2908 0.545408 0 -10 1.53706 1.11561 #255#) 
COMMENT                             #257=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #126# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    2.58688 3.23435 1 0.082322) 0.8 1.8 1) 
COMMENT                             #258=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #200# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "ampicillin resistance" "@N" 1 -7.45429 
COMMENT                                   -12.3272 0 -10 8.85048 1.11561 #257#) 
COMMENT                             #259=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #126# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    4.43755 6.21528 1 0.082322) 0.8 1.8 1) 
COMMENT                             #260=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #200# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "lacZ delta-omega fragment" "@N" 1 18.6519 
COMMENT                                   10.7624 0 -10 11.6271 1.11561 #259#) 
COMMENT                             #261=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #18# 0) 1 2 0 0 #126# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    0.303056 1.11747 1 0.082322) 0.8 1.8 1) 
COMMENT                             #262=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #200# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lacI" "@N" 1 
COMMENT                                   -10.8067 12.8091 0 -10 1.53706 1.11561 #261#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #263=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -254 100) 
COMMENT                            (CObjectList 
COMMENT                             #264=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #145# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 1.16464 6.2087 0.012784) 
COMMENT                                   0.4 0.082322 1.08464 1.8 1.8 0) 
COMMENT                             #265=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #200# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 promoter" 
COMMENT                                   "@N" 1 3.63522 15.9715 0 -10 5.3797 1.11561 
COMMENT                                   #264#) 
COMMENT                             #266=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #145# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 0.835356 4.36461 4.45188) 
COMMENT                                   0.4 0.082322 0.915356 1.8 1.8 1) 
COMMENT                             #267=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #200# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "tac promoter" 
COMMENT                                   "@N" 1 19.1947 -5.72282 0 -10 5.55324 1.11561 
COMMENT                                   #266#) 
COMMENT                             #268=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #145# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 0.670712 4.31648 4.40375) 
COMMENT                                   0.4 0.082322 0.750712 1.8 1.8 1) 
COMMENT                             #269=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #200# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SP6 promoter" 
COMMENT                                   "@N" 1 19.2422 -9.57934 0 -10 6.17302 1.11561 
COMMENT                                   #268#) 
COMMENT                             #270=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #211# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    4.19541 4.25858 1 0.082322) 0.8 1.8 1) 
COMMENT                             #271=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #200# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lac promoter" 
COMMENT                                   "@N" 1 18.1417 -11.162 0 -10 5.50366 1.11561 
COMMENT                                   #270#) 
COMMENT                             #272=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #19# 0) 1 2 0 0 #211# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    0.2444 0.303056 1 0.082322) 0.8 1.8 1) 
COMMENT                             #273=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #200# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lacI promoter" 
COMMENT                                   "@N" 1 -7.42811 14.7994 0 -10 5.6772 1.11561 
COMMENT                                   #272#)) (CObjectList)) 
COMMENT                      #274=(CGroupWidget 
COMMENT                            (CWidget 32 (7 32 0) 1 2 0 0 Nil -1167 100) 
COMMENT                            (CObjectList 
COMMENT                             #275=(CScratch 
COMMENT                                   (CWidget 0 (3 #17# 0) 1 2 0 0 #143# 35511068 
COMMENT                                    100) (LOGPEN 0 6 16711680) 8 1 4.39168 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #276=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #201# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' UTR of lacZ gene, incl. RBS" "@N" 1 
COMMENT                                   23.2411 -4.58936 0 -10 12.9906 1.11561 #275#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #277=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -464 100) 
COMMENT                            (CObjectList 
COMMENT                             #278=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #138# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    1.87549 2.17854 1 0.082322) 0.8 1.8 0) 
COMMENT                             #279=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #200# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 -12.9465 -5.64844 0 -10 4.06577 1.11561 
COMMENT                                   #278#) 
COMMENT                             #280=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #138# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    3.32008 3.66299 1 0.082322) 0.8 1.8 0) 
COMMENT                             #281=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #200# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "f1 ori" "@N" 1 
COMMENT                                   6.22873 -13.9029 0 -10 2.2808 1.11561 #280#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #282=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -183 100) 
COMMENT                            (CObjectList 
COMMENT                             #283=(CScratch 
COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #129# 914 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 1 3.72616 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #284=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #227# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 terminator" 
COMMENT                                   "@N" 1 10.8683 -12.0119 0 -10 5.80115 1.11561 
COMMENT                                   #283#)) (CObjectList))) (CObjectList)) 
COMMENT               #285=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -746 100) 
COMMENT                     (CObjectList 
COMMENT                      #286=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -1309 100) 
COMMENT                            (CObjectList 
COMMENT                             #287=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #247# 914 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 4.43229 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #288=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #232# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Bam}HI (2449)" "@N (@S)" 5 19.5235 
COMMENT                                   -1.12255 0 -10 5.47887 1.06602 #287#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #289=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #4# 0) 1 2 0 0 Nil -887 100) 
COMMENT                            (CObjectList 
COMMENT                             #290=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #247# 46450664 
COMMENT                                    100) (LOGPEN 0 0 16724736) 1 1 4.32626 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #291=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #232# 45350924 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Hin}dIII (2590)" "@N (@S)" 5 19.3478 
COMMENT                                   -8.35367 0 -10 5.60282 1.06602 #290#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #292=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #5# 0) 1 2 0 0 Nil -957 100) 
COMMENT                            (CObjectList 
COMMENT                             #293=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #247# 45946116 
COMMENT                                    100) (LOGPEN 0 0 16724736) 1 1 6.2213 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #294=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #232# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Nco}I (70)" "@N (@S)" 5 4.14278 14.4275 
COMMENT                                   0 -10 3.81785 1.06602 #293#)) (CObjectList)) 
COMMENT                      #295=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #6# 0) 1 2 0 0 Nil -184 100) 
COMMENT                            (CObjectList 
COMMENT                             #296=(CScratch 
COMMENT                                   (CWidget 1 (1 #6# 1) 1 2 0 0 #246# 2082602612 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 5.90471 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #297=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #245# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Sac}I (491)" "@N (@S)" 5 7.2229 13.1314 
COMMENT                                   0 -10 4.11535 1.06602 #296#) 
COMMENT                             #298=(CScratch 
COMMENT                                   (CWidget 2 (1 #6# 2) 1 2 0 0 #246# 928 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 4.42026 1.9 0.082322 1) 
COMMENT                             #299=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #245# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Sac}I (2465)" "@N (@S)" 5 19.2598 
COMMENT                                   -2.31947 0 -10 4.56159 1.06602 #298#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #300=(CGroupWidget 
COMMENT                            (CWidget 4 (10 #3# 0) 1 2 0 0 Nil -1379 100) 
COMMENT                            (CObjectList 
COMMENT                             #301=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #246# 35496764 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 5.23843 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #302=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #245# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (1377)" "@N (@S)" 5 16.7705 
COMMENT                                   9.06963 0 -10 5.28054 1.06602 #301#) 
COMMENT                             #303=(CScratch 
COMMENT                                   (CWidget 2 (1 #3# 2) 1 2 0 0 #246# 915 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 4.76918 1.9 0.082322 1) 
COMMENT                             #304=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #245# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (2001)" "@N (@S)" 5 17.5846 
COMMENT                                   2.15684 0 -10 5.28054 1.06602 #303#) 
COMMENT                             #305=(CScratch 
COMMENT                                   (CWidget 3 (1 #3# 3) 1 2 0 0 #246# 914 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 4.34054 1.9 0.082322 1) 
COMMENT                             #306=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #245# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (2571)" "@N (@S)" 5 19.1175 
COMMENT                                   -7.15674 0 -10 5.28054 1.06602 #305#) 
COMMENT                             #307=(CScratch 
COMMENT                                   (CWidget 4 (1 #3# 4) 1 2 0 0 #246# 915 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 0.94752 1.9 0.082322 1) 
COMMENT                             #308=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #245# 2081718712 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (7083)" "@N (@S)" 5 -13.9775 
COMMENT                                   8.71165 0 -10 5.28054 1.06602 #307#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #309=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1239 100) 
COMMENT                     (CObjectList 
COMMENT                      #310=(CLabel 
COMMENT                            (CWidget 0 (15 #20# 0) 1 2 0 0 #151# 842548548 100) 
COMMENT                            (LOGPEN 877016877 1127233334 1178874434) 908935992 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 6701" 
COMMENT                            "LMBP 6701" 65535 0.123956 -4.46243 0 -10 7.41259 
COMMENT                            1.56185 Nil) 
COMMENT                      #311=(CLabel 
COMMENT                            (CWidget 0 (15 #21# 0) 1 2 0 0 #151# 539780469 100) 
COMMENT                            (LOGPEN 840986229 540029232 875706928) 1414350624 
COMMENT                            (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 12-JUL-2010" 
COMMENT                            "Last modified: 12-JUL-2010" 65535 0.123956 -6.07386 
COMMENT                            0 -10 11.1313 1.06602 Nil)) (CObjectList)) 
COMMENT               #312=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1028 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 
COMMENT       (CStringList) 0 0 (CObList)))
FEATURES             Location/Qualifiers
     rep_origin      5446..5848
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(6094..6285)
                     /vntifkey="4"
                     /label=rop
     promoter        8326..8342
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(2539..2573)
                     /vntifkey="30"
                     /label=tac\promoter
     promoter        complement(2603..2619)
                     /vntifkey="30"
                     /label=SP6\promoter
     promoter        complement(2680..2763)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      3388..3435
                     /vntifkey="43"
                     /label=T7\terminator
     rep_origin      3472..3927
                     /vntifkey="33"
                     /label=f1\ori
     CDS             complement(4042..4902)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             complement(78..2441)
                     /vntifkey="4"
                     /label=lacZ\delta-omega\fragment
     promoter        complement(7940..8017)
                     /vntifkey="30"
                     /label=lacI\promoter
                     /note="repressor lacI promoter, incl. RBS"
     CDS             complement(6857..7939)
                     /vntifkey="4"
                     /label=lacI
     RBS             complement(2503..2538)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ\gene,\incl.\RBS
BASE COUNT     1903 a      2253 c      2198 g      1988 t 
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag 
       61 gagatatacc atggttatgc acgggtgaac tgatcgcgca gcggcgtcag cagttgtttt 
      121 ttatcgccaa tccacatctg tgaaagaaag cctgactggc ggttaaattg ccaacgctta 
      181 ttacccagct cgatgcaaaa atccatttcg ctggtggtca gatgcgggat ggcgtgggac 
      241 gcggcgggga gcgtcacact gaggttttcc gccagacgcc actgctgcca ggcgctgatg 
      301 tgcccggctt ctgaccatgc ggtcgcgttc ggttgcacta cgcgtactgt gagccagagt 
      361 tgcccggcgc tctccggctg cggtagttca ggcagttcaa tcaactgttt accttgtgga 
      421 gcgacatcca gaggcacttc accgcttgcc agcggcttac catccagcgc caccatccag 
      481 tgcaggagct cgttatcgct atgacggaac aggtattcgc tggtcacttc gatggtttgc 
      541 ccggataaac ggaactggaa aaactgctgc tggtgttttg cttccgtcag cgctggatgc 
      601 ggcgtgcggt cggcaaagac cagaccgttc atacagaact ggcgatcgtt cggcgtatcg 
      661 ccaaaatcac cgccgtaagc cgaccacggg ttgccgtttt catcatattt aatcagcgac 
      721 tgatccaccc agtcccagac gaagccgccc tgtaaacggg gatactgacg aaacgcctgc 
      781 cagtatttag cgaaaccgcc aagactgtta cccatcgcgt gggcgtattc gcaaaggatc 
      841 agcgggcgcg tctctccagg tagcgaaagc cattttttga tggaccattt cggcacagcc 
      901 gggaagggct ggtcttcatc cacgcgcgcg tacatcgggc aaataatatc ggtggccgtg 
      961 gtgtcggctc cgccgccttc atactgcacc gggcgggaag gatcgacaga tttgatccag 
     1021 cgatacagcg cgtcgtgatt agcgccgtgg cctgattcat tccccagcga ccagatgatc 
     1081 acactcgggt gattacgatc gcgctgcacc attcgcgtta cgcgttcgct catcgccggt 
     1141 agccagcgcg gatcatcggt cagacgattc attggcacca tgccgtgggt ttcaatattg 
     1201 gcttcatcca ccacatacag gccgtagcgg tcgcacagcg tgtaccacag cggatggttc 
     1261 ggataatgcg aacagcgcac ggcgttaaag ttgttctgct tcatcagcag gatatcctgc 
     1321 accatcgtct gctcatccat gacctgacca tgcagaggat gatgctcgtg acggttaacg 
     1381 cctcgaatca gcaacggctt gccgttcagc agcagcagac cattttcaat ccgcacctcg 
     1441 cggaaaccga catcgcaggc ttctgcttca atcagcgtgc cgtcggcggt gtgcagttca 
     1501 accaccgcac gatagagatt cgggatttcg gcgctccaca gtttcgggtt ttcgacgttc 
     1561 agacgtagtg tgacgcgatc ggcataacca ccacgctcat cgataatttc accgccgaaa 
     1621 ggcgcggtgc cgctggcgac ctgcgtttca ccctgccata aagaaactgt tacccgtagg 
     1681 tagtcacgca actcgccgca catctgaact tcagcctcca gtacagcgcg gctgaaatca 
     1741 tcattaaagc gagtggcaac atggaaatcg ctgatttgtg tagtcggttt atgcagcaac 
     1801 gagacgtcac ggaaaatgcc gctcatccgc cacatatcct gatcttccag ataactgccg 
     1861 tcactccaac gcagcaccat caccgcgagg cggttttctc cggcgcgtaa aaatgcgctc 
     1921 aggtcaaatt cagacggcaa acgactgtcc tggccgtaac cgacccagcg cccgttgcac 
     1981 cacagatgaa acgccgagtt aacgccatca aaaataattc gcgtctggcc ttcctgtagc 
     2041 cagctttcat caacattaaa tgtgagcgag taacaacccg tcggattctc cgtgggaaca 
     2101 aacggcggat tgaccgtaat gggataggtt acgttggtgt agatgggcgc atcgtaaccg 
     2161 tgcatctgcc agtttgaggg gacgacgaca gtatcggcct caggaagatc gcactccagc 
     2221 cagctttccg gcaccgcttc tggtgccgga aaccaggcaa agcgccattc gccattcagg 
     2281 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg 
     2341 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga 
     2401 cgttgtaaaa cgacggccag tgaatccgta atcatggtca tacccccgga tccgaattcg 
     2461 agctcggcgc gcctgcagcg gccgcatgca tatggatcga tcctgtttcc tgtgtgaaat 
     2521 tgttatccgc tcacaattcc acacattata cgagccgatg attaattgtc aacaggggga 
     2581 tggggagtaa gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca 
     2641 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 
     2701 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 
     2761 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 
     2821 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 
     2881 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 
     2941 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgaattaatt ctcatgtttg 
     3001 acagcttatc atcggataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt 
     3061 ctcatgtttg acagcttatc ttcgattagc tttaatgcgg tagtttatca cagttaaatt 
     3121 gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc atcctcggca 
     3181 ccgtcaccct ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc 
     3241 gggatatcgg ccggccacgc gatcgctgac gtcggtaccc tcgagtctgg taaagaaacc 
     3301 gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagcgcagc ttaattaacc 
     3361 taggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc 
     3421 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct 
     3481 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 
     3541 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 
     3601 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 
     3661 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 
     3721 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 
     3781 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 
     3841 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 
     3901 ttaacaaaat attaacgttt acaatttctg gcggcacgat ggcatgagat tatcaaaaag 
     3961 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
     4021 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
     4081 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
     4141 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
     4201 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
     4261 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
     4321 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 
     4381 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
     4441 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
     4501 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
     4561 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
     4621 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 
     4681 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
     4741 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
     4801 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
     4861 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaatc 
     4921 atgattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     4981 agaaaaataa acaaataggt catgaccaaa atcccttaac gtgagttttc gttccactga 
     5041 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 
     5101 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 
     5161 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 
     5221 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 
     5281 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 
     5341 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 
     5401 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 
     5461 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 
     5521 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 
     5581 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 
     5641 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 
     5701 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 
     5761 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 
     5821 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg 
     5881 tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat 
     5941 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca 
     6001 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 
     6061 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 
     6121 acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc 
     6181 tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct 
     6241 gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta 
     6301 agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat 
     6361 acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc 
     6421 ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa 
     6481 tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat 
     6541 aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac 
     6601 cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc 
     6661 gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct 
     6721 caacgacagg agcacgatca tgctagtcat gccccgcgcc caccggaagg agctgactgg 
     6781 gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac 
     6841 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 
     6901 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt 
     6961 ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga 
     7021 gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg 
     7081 ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatgt 
     7141 ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat 
     7201 cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt 
     7261 gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc 
     7321 gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc 
     7381 ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg 
     7441 taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa 
     7501 ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg 
     7561 gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac 
     7621 aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg 
     7681 cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg 
     7741 caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt 
     7801 aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg 
     7861 cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt 
     7921 ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg 
     7981 ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta 
     8041 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc 
     8101 gcaaggaatg gtgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca 
     8161 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg 
     8221 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga 
     8281 tgcgtccggc gtagaggatc gagatcgatc tcgatcccgc gaaattaata cgactcacta 
     8341 ta 
//
