LOCUS       pEXPR-E-slrP            6512 bp    DNA     circular      4-FEB-2011
DEFINITION  LMBP 6729.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6729
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 4-FEB-2011
            
            
            
            
COMMENT     VNTDATE|561818298|
COMMENT     VNTNAME|pEXPR-E-slrP|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "HindIII" "NarI" "SacI" "SacII" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (CMolPar 0 0 0 389 445 1 6512 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 4040 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 4 271 0 3605 0 4673 0 5910 
COMMENT            0 0 "") #2=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 6476 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1 269 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 24 0 5630 0 0 "")) 
COMMENT       (CObList 
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COMMENT            (CStringList) 1 1 1 1 "4181..4201") 
COMMENT        #6=(CFSignal (CObList) "attB2" 0 0 1 6505 13 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6505..13)") 
COMMENT        #7=(CFSignal (CObList) "lacUV5 promoter" 30 0 1 389 445 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "389..445") 
COMMENT        #8=(CFSignal (CObList) "E-tag" 22 0 0 4111 4149 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4111..4149") 
COMMENT        #9=(CFSignal (CObList) "sCMV-IE promoter and enhancer" 29 0 0 3027 4039 
COMMENT            0 (CStringList) (CStringList) 1 1 1 1 "3027..4039") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 0 120 216 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 1 120 216 0 
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COMMENT        #12=(CFSignal (CObList) "T7 promoter" 30 0 1 39 55 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "SP6 promoter" 30 0 0 4046 4062 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "T3 promoter" 30 0 1 309 325 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "f1 ori" 33 0 1 2498 2953 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "ampicillin resistance" 4 0 1 1507 2367 0 
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COMMENT        #17=(CFSignal (CObList) "pMB1 ori" 33 0 1 564 966 0 (CStringList) 
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COMMENT                   "Standard Fields") 
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COMMENT                                        "Simian cytomegalovirus immediate early promoter (CMV-IE) and enhancer" 
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COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                        "Start: 39    End: 55   (Complementary)" 
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COMMENT                                        "Escherichia coli lac operon promoter; mutant (lacUV5)" 
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COMMENT                                        "   389..445" 1))) "lacUV5 promoter") 
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COMMENT                                        "Escherichia coli plasmid pMB1 origin" 1) 
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COMMENT                    "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     attenuator      4181..4201
                     /vntifkey="0"
                     /label=attB1
     attenuator      complement(6505..13)
                     /vntifkey="0"
                     /label=attB2
     promoter        complement(389..445)
                     /vntifkey="30"
                     /label=lacUV5\promoter
                     /note="Escherichia coli lac operon promoter; mutant (lacUV5)"
     misc_marker     4111..4149
                     /vntifkey="22"
                     /label=E-tag
                     /note="N-terminal E-tag"
     promoter        3027..4039
                     /vntifkey="29"
                     /label=sCMV-IE\promoter\and\enhancer
                     /note="Simian cytomegalovirus immediate early promoter (CMV-IE) and enhancer"
     terminator      120..216
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      complement(120..216)
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     promoter        complement(39..55)
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="Phage T7 gene 10 promoter (T7g10)"
     promoter        4046..4062
                     /vntifkey="30"
                     /label=SP6\promoter
                     /note="Phage SP6 promoter"
     promoter        complement(309..325)
                     /vntifkey="30"
                     /label=T3\promoter
                     /note="Phage T3 promoter"
     rep_origin      complement(2498..2953)
                     /vntifkey="33"
                     /label=f1\ori
                     /note="Phage f1 origin"
     CDS             complement(1507..2367)
                     /vntifkey="4"
                     /label=ampicillin\resistance
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(564..966)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="Escherichia coli plasmid pMB1 origin"
     CDS             4204..6501
                     /vntifkey="4"
                     /label=SlrP
BASE COUNT     1761 a      1511 c      1612 g      1628 t 
ORIGIN
        1 ttgtacaaag tggttcgatc ctctcgagcc tctagaacta tagtgagtcg tattacgtag 
       61 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa 
      121 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct 
      181 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg 
      241 tgtgggaggt tttttaattc gcggccgcgg cgccaatgca ttgggcccgg tacccagctt 
      301 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc 
      361 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
      421 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
      481 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 
      541 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
      601 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
      661 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
      721 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
      781 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
      841 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
      901 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
      961 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     1021 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     1081 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     1141 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 
     1201 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     1261 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
     1321 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
     1381 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 
     1441 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 
     1501 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 
     1561 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 
     1621 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 
     1681 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 
     1741 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 
     1801 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 
     1861 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 
     1921 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 
     1981 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 
     2041 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 
     2101 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 
     2161 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 
     2221 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 
     2281 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 
     2341 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 
     2401 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 
     2461 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg 
     2521 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 
     2581 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 
     2641 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 
     2701 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 
     2761 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 
     2821 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 
     2881 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 
     2941 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 
     3001 cgggcctctt cgctattacg ccagtcgacc atagccaatt caatatggcg tatatggact 
     3061 catgccaatt caatatggtg gatctggacc tgtgccaatt caatatggcg tatatggact 
     3121 cgtgccaatt caatatggtg gatctggacc ccagccaatt caatatggcg gacttggcac 
     3181 catgccaatt caatatggcg gacttggcac tgtgccaact ggggaggggt ctacttggca 
     3241 cggtgccaag tttgaggagg ggtcttggcc ctgtgccaag tccgccatat tgaattggca 
     3301 tggtgccaat aatggcggcc atattggcta tatgccagga tcaatatata ggcaatatcc 
     3361 aatatggccc tatgccaata tggctattgg ccaggttcaa tactatgtat tggccctatg 
     3421 ccatatagta ttccatatat gggttttcct attgacgtag atagcccctc ccaatgggcg 
     3481 gtcccatata ccatatatgg ggcttcctaa taccgcccat agccactccc ccattgacgt 
     3541 caatggtctc tatatatggt ctttcctatt gacgtcatat gggcggtcct attgacgtat 
     3601 atggcgcctc ccccattgac gtcaattacg gtaaatggcc cgcctggctc aatgcccatt 
     3661 gacgtcaata ggaccaccca ccattgacgt caatgggatg gctcattgcc cattcatatc 
     3721 cgttctcacg ccccctattg acgtcaatga cggtaaatgg cccacttggc agtacatcaa 
     3781 tatctattaa tagtaacttg gcaagtacat tactattgga aggacgccag ggtacattgg 
     3841 cagtactccc attgacgtca atggcggtaa atggcccgcg atggctgcca agtacatccc 
     3901 cattgacgtc aatggggagg ggcaatgacg caaatgggcg ttccattgac gtaaatgggc 
     3961 ggtaggcgtg cctaatggga ggtctatata agcaatgctc gtttagggaa ccgccattct 
     4021 gcctggggac gtcggagcaa gcttgattta ggtgacacta tagaatacaa gctacttgtt 
     4081 ctttttgcag gatcccatcg atcccccatg ggtgcgccgg tgccgtatcc ggacccgctg 
     4141 gaaccgcgtg cggcccatcg attcgaattc aaggatcaaa caagtttgta caaaaaagca 
     4201 ggcttgttta atattactaa tatacaatct acggcaaggc atcaaagtat tagcaatgag 
     4261 gcctcaacag aggtgccttt aaaagaagag atatggaata aaataagtgc ctttttctct 
     4321 tcagaacatc aggttgaagc acaaaactgc atcgcttatc tttgtcatcc acctgaaacc 
     4381 gcctcgccag aagagatcaa aagcaagttt gaatgtttaa ggatgttagc tttcccggcg 
     4441 tatgcggata atattcagta tagtagagga ggggcagacc aatactgtat tttgagtgaa 
     4501 aatagtcagg aaattctgtc tatagttttt aatacagagg gctataccgt tgagggaggg 
     4561 ggaaagtcag tcacctatac ccgtgtgaca gaaagcgagc aggcgagtag cgcttccggc 
     4621 tccaaagatg ctgtgaatta tgagttaatc tggtctgagt gggtaaaaga ggcgccagcg 
     4681 aaagaggcag caaatcgtga agaagccgta caacggatgc gtgactgcct gaaaaataat 
     4741 aagacggaac ttcgtctgaa aatattagga cttaccacta tacctgccta tattcctgag 
     4801 cagataacta ctctgatact cgataacaat gaactgaaaa gtttgccgga aaatttacag 
     4861 ggaaatataa agaccctgta tgccaacagt aatcagctaa ccagtatccc tgccacgtta 
     4921 ccggatacca tacaggaaat ggagctgagc attaaccgta ttactgaatt gccggaacgt 
     4981 ttgccttcag cgcttcaatc gctggatctt ttccataata aaattagttg cttacctgaa 
     5041 aatctacctg aggaacttcg gtacctgagc gtttatgata acagcataag gacactgcca 
     5101 gcacatcttc cgtcagggat tacccatttg aatgtgcaga gtaattcgtt aaccgctttg 
     5161 cctgaaacat tgccgccggg cctgaagact ctggaggccg gcgaaaatgc cttaaccagt 
     5221 ctgcccgcat cgttaccacc agaattacag gtcctggatg taagtaaaaa tcagattacg 
     5281 gttctgcctg aaacacttcc tcccacgata acaacgctgg atgtttcccg taacgcattg 
     5341 actaatctac cggaaaacct cccggcggca ttacaaataa tgcaggcctc tcgcaataac 
     5401 ctggtccgtc tcccggagtc gttaccccat tttcgtggtg aaggacctca acctacaaga 
     5461 ataatcgtag aatataatcc tttttcagaa cgaacaatac agaatatgca gcggctaatg 
     5521 tcctctgtag attatcaggg accccgggta ttgtttgcca tgggcgactt ttcaattgtt 
     5581 cgggtaactc gaccactgca tcaagctgtc caggggtggc taaccagtct cgaggaggaa 
     5641 gacgtcaacc aatggcgggc gtttgaggca gaggcaaacg cggcggcttt cagcggattc 
     5701 ctggactatc ttggtgatac gcagaatacc cgacacccgg attttaagga acaagtctcc 
     5761 gcctggctaa tgcgcctggc tgaagatagc gcactaagag aaaccgtatt tattatagcg 
     5821 atgaatgcaa cgataagctg tgaagatcgg gtcacactgg cataccacca aatgcaggaa 
     5881 gcgacgttgg ttcatgatgc tgaaagaggc gcctttgata gccacttagc ggaactgatt 
     5941 atggcggggc gtgaaatctt tcggctggag caaatagaat cgctcgccag agaaaaggta 
     6001 aaacggctgt tttttattga cgaagtcgaa gtatttctgg ggtttcagaa tcagttacga 
     6061 gagtcgctgt cgctgacaac aatgacccgg gatatgcgat tttataacgt ttcgggtatc 
     6121 actgagtctg acctggacga ggcggaaata aggataaaaa tggctgaaaa tagggatttt 
     6181 cacaaatggt ttgcgctgtg ggggccgtgg cataaagtgc tggagcgcat agcgccagaa 
     6241 gagtggcgtg aaatgatggc taaaagggat gagtgtattg aaacggatga gtatcagagc 
     6301 cgggtcaatg ctgaactgga agatttaaga atagcagacg actctgacgc agagcgtact 
     6361 actgaggtac agatggatgc agagcgtgct attgggataa aaataatgga agagatcaat 
     6421 cagaccctct ttactgagat catggagaat atattgctga aaaaagaggt gagctcgctc 
     6481 atgagcgcct actggcgata gacccagctt tc 
//
