ID pLenti6(puro)-EYFP standard; circular DNA; ; 10090 BP. DE LMBP 6783. CC Accession number: LMBP 6783 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 18-MAY-2011 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS complement(1193..1852) FT /vntifkey="4" FT /label=chloramphenicol\resistance FT CDS complement(546..851) FT /vntifkey="4" FT /label=ccdB FT promoter 3079..3361 FT /vntifkey="29" FT /label=SV40\early\promoter FT misc_marker 3005..3046 FT /vntifkey="22" FT /label=V5\epitope FT terminator 4469..4543 FT /vntifkey="43" FT /label=SV40\polyA\early FT promoter 7725..8053 FT /vntifkey="29" FT /label=RSV/HIV-1\5'\LTR FT /note="RSV promoter: 7725-7872 / HIV-1 5' LTR: 7873-8053" FT rep_origin 4972..5427 FT /vntifkey="33" FT /label=f1\origin FT promoter 9450..9966 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT CDS 3470..4072 FT /vntifkey="4" FT /label=puromycin\resistance FT CDS 5558..6418 FT /vntifkey="4" FT /label=ampicillin\resistance FT CDS 2274..2993 FT /vntifkey="4" FT /label=EYFP FT attenuator complement(10083..117) FT /vntifkey="0" FT /label=attR1 FT attenuator 2133..2257 FT /vntifkey="0" FT /label=attR2 FT rep_origin 6959..7361 FT /vntifkey="33" FT /label=pMB1\ori FT promoter 7453..7536 FT /vntifkey="30" FT /label=lac\promoter FT promoter 7600..7616 FT /vntifkey="30" FT /label=T3\promoter FT terminator complement(4469..4543) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin 3318..3393 FT /vntifkey="33" FT /label=SV40\ori FT rep_origin 4679..4754 FT /vntifkey="33" FT /label=SV40\ori FT promoter complement(4788..4804) FT /vntifkey="30" FT /label=T7\promoter FT misc_feature 8164..8208 FT /vntifkey="21" FT /label=HIV-1\psi\packaging\signal FT misc_feature 8718..8951 FT /vntifkey="21" FT /label=HIV-1\Rev\response\element FT terminator 4163..4397 FT /vntifkey="43" FT /label=RSV/HIV-1\dU3/3'\LTR FT /note="dU3: 4163-4216 / 3' LTR: 4217-4397" FT misc_feature 4147..4162 FT /vntifkey="21" FT /label=HIV-1\cPPT SQ Sequence 10090 BP; 2626 A; 2531 C; 2476 G; 2457 t; gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat 60 tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt cactatgaat 120 caactactta gatggtatta gtgacctgta gtcgaccgac agccttccaa atgttcttcg 180 ggtgatgctg ccaacttagt cgaccgacag ccttccaaat gttcttctca aacggaatcg 240 tcgtatccag cctactcgct attgtcctca atgccgtatt aaatcataaa aagaaataag 300 aaaaagaggt gcgagcctct tttttgtgtg acaaaataaa aacatctacc tattcatata 360 cgctagtgtc atagtcctga aaatcatctg catcaagaac aatttcacaa ctcttatact 420 tttctcttac aagtcgttcg gcttcatctg gattttcagc ctctatactt actaaacgtg 480 ataaagtttc tgtaatttct actgtatcga cctgcagact ggctgtgtat aagggagcct 540 gacatttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg 600 ctgagatcag ccacttcttc cccgataacg gagaccggca cactggccat atcggtggtc 660 atcatgcgcc agctttcatc cccgatatgc accaccgggt aaagttcacg ggagacttta 720 tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata 780 atatcactct gtacatccac aaacagacga taacggctct ctcttttata ggtgtaaacc 840 ttaaactgca tttcaccagt ccctgttctc gtcagcaaaa gagccgttca tttcaataaa 900 ccgggcgacc tcagccatcc cttcctgatt ttccgctttc cagcgttcgg cacgcagacg 960 acgggcttca ttctgcatgg ttgtgcttac cagaccggag atattgacat catatatgcc 1020 ttgagcaact gatagctgtc gctgtcaact gtcactgtaa tacgctgctt catagcacac 1080 ctctttttga catacttcgg gtatacatat cagtatatat tcttataccg caaaaatcag 1140 cgcgcaaata cgcatactgt tatctggctt ttagtaagcc ggatccacgc gattacgccc 1200 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc 1260 catcacagac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg 1320 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta 1380 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa 1440 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt 1500 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt 1560 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt 1620 tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg 1680 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct 1740 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac 1800 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt 1860 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat 1920 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg 1980 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt 2040 ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac ttagtcgact 2100 acaggtcact aataccatct aagtagttga ttcatagtga ctggatatgt tgtgttttac 2160 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat 2220 tttacgtttc tcgttcagct ttcttgtaca aagtggttga tccggtcgcc accatggtga 2280 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg 2340 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc 2400 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga 2460 ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg 2520 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg 2580 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc 2640 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg 2700 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca 2760 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact 2820 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga 2880 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg 2940 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc 3000 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta 3060 atgagtttgg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 3120 tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 3180 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 3240 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 3300 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc 3360 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 3420 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgacca tggccaccga 3480 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct 3540 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat 3600 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 3660 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 3720 agcgggggcg gtgttcgccg agatcggctc gcgcatggcc gagttgagcg gttcccggct 3780 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 3840 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 3900 cgtgctcccc ggagtggagg cggccgagcg cgctggggtg cccgccttcc tggagacctc 3960 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 4020 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagctaagca 4080 caattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 4140 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct 4200 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 4260 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 4320 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 4380 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 4440 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 4500 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 4560 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta 4620 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 4680 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 4740 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt 4800 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 4860 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 4920 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct 4980 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 5040 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 5100 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 5160 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 5220 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 5280 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 5340 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 5400 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac 5460 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 5520 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 5580 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 5640 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 5700 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 5760 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 5820 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 5880 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 5940 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 6000 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 6060 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 6120 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 6180 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 6240 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 6300 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 6360 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 6420 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 6480 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 6540 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 6600 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 6660 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 6720 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 6780 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 6840 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 6900 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 6960 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 7020 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 7080 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 7140 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 7200 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 7260 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 7320 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc 7380 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 7440 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 7500 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 7560 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga 7620 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac 7680 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga 7740 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca 7800 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct 7860 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 7920 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 7980 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 8040 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc 8100 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac 8160 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga 8220 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag 8280 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat 8340 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc 8400 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa 8460 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga 8520 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga 8580 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta 8640 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa 8700 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact 8760 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg 8820 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca 8880 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat 8940 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct 9000 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg 9060 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg 9120 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg 9180 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta 9240 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg 9300 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg 9360 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg 9420 aacggatctc gacggtatcg ataagcttgg gagttccgcg ttacataact tacggtaaat 9480 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 9540 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 9600 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 9660 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 9720 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 9780 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 9840 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 9900 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 9960 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc 10020 catagaagac accgactcta gaggatccac tagtccagtg tggtggaatt ctgcagatat 10080 caacaagttt 10090 //