LOCUS       pMSCV-His-AU-Ub-        6584 bp    DNA     circular      1-MAR-2018
DEFINITION  LMBP 6801.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|543580266|
COMMENT     VNTDBDATE|-12182017|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMSCV-His-AU-Ub-wt|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Yabru|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     misc_feature    1424..1450
                     /vntifkey="21"
                     /label=His-tag
     misc_feature    1451..1480
                     /vntifkey="21"
                     /label=AU1-tag
     CDS             1481..1711
                     /vntifkey="4"
                     /label=hUbB
     terminator      2947..3459
                     /vntifkey="43"
                     /label=3'\MSCV-PCMV\LTR
     promoter        1720..2227
                     /vntifkey="29"
                     /label=mPGK1\promoter
     CDS             2247..2846
                     /vntifkey="4"
                     /label=puromycin\resistance
     CDS             complement(4790..5650)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(3847..4249)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(3672..3755)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        1..513
                     /vntifkey="29"
                     /label=5'\MSCV-PCMV\LTR
     misc_feature    516..1404
                     /vntifkey="21"
                     /label=psi+\extended\packaging\signal
BASE COUNT     1496 a      1879 c      1741 g      1468 t 
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 
     1381 ccctcactcc ttctctaggc gccggaatta gatcaagctt aaaatggtac atcaccatca 
     1441 ccatcaccat gccatggaca cataccgcta catcggatcc atgcagatct tcgtgaaaac 
     1501 cctgaccggc aagaccatca ctctggaggt ggagcccagt gacaccatcg aaaatgtgaa 
     1561 ggccaagatc caagataaag aaggcatccc ccccgaccag cagaggctca tctttgcagg 
     1621 caagcagctg gaagatggcc gcactctttc tgactacaac atccagaaag agtcgaccct 
     1681 gcacctggtc ctgcgtctca gaggtgggta ggggcccaat tctaccgggt aggggaggcg 
     1741 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac 
     1801 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg 
     1861 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc 
     1921 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc 
     1981 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa 
     2041 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc 
     2101 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca 
     2161 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc 
     2221 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca 
     2281 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg 
     2341 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac 
     2401 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg 
     2461 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg 
     2521 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc 
     2581 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc 
     2641 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg 
     2701 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg 
     2761 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt 
     2821 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa 
     2881 ggagcgcacg accccatgca tcgataaaat aaaagatttt atttagtctc cagaaaaagg 
     2941 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca 
     3001 aggcatggaa aatacataac tgagaataga gaagttcaga tcaaggttag gaacagagag 
     3061 acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg 
     3121 ccaagaacag atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag 
     3181 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat 
     3241 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca 
     3301 caacccctca ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt acccgtgtat 
     3361 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct 
     3421 cctctgagtg attgactacc cgtcagcggg ggtctttcat gggtaacagt ttcttgaagt 
     3481 tggagaacaa cattctgagg gtaggagtcg aatattaagt aatcctgact caattagcca 
     3541 ctgttttgaa tccacatact ccaatactcc tgaaatagtt cattatggac agcgcagaag 
     3601 agctggggag aattaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc 
     3661 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 
     3721 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 
     3781 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     3841 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     3901 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 
     3961 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 
     4021 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 
     4081 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 
     4141 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 
     4201 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 
     4261 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 
     4321 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 
     4381 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 
     4441 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 
     4501 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 
     4561 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 
     4621 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 
     4681 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 
     4741 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 
     4801 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 
     4861 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 
     4921 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 
     4981 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 
     5041 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 
     5101 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 
     5161 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 
     5221 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 
     5281 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 
     5341 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 
     5401 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 
     5461 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 
     5521 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 
     5581 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 
     5641 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 
     5701 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 
     5761 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 
     5821 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc 
     5881 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 
     5941 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 
     6001 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 
     6061 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg 
     6121 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 
     6181 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 
     6241 cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc 
     6301 caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc 
     6361 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc 
     6421 cacgatgcgt ccggcgtaga ggcgattagt ccaatttgtt aaagacagga tatcagtggt 
     6481 ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata 
     6541 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaa 
//
