LOCUS       pEF6-F-birA             6698 bp    DNA     circular     13-AUG-2009
DEFINITION  LMBP 6811.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|525964895|
COMMENT     VNTNAME|pEF6-F-birA|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     promoter        complement(6604..6632)
                     /vntifkey="30"
                     /label=AmpR_promoter
     gene            complement(5702..6562)
                     /gene="Ampicillin"
                     /vntifkey="60"
     CDS             complement(5702..6562)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 2"
     rep_origin      complement(4928..5547)
                     /vntifkey="33"
                     /label=pBR322_origin
     promoter        complement(4601..4630)
                     /vntifkey="30"
                     /label=lac_promoter
     misc_feature    complement(4565..4587)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     misc_feature    complement(4548..4566)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    4473..4492
                     /vntifkey="21"
                     /label=EBV_rev_primer
     terminator      4385..4504
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     gene            3826..4224
                     /gene="blast"
                     /vntifkey="60"
     promoter        3758..3825
                     /vntifkey="30"
                     /label=EM7_promoter
     misc_feature    3625..3644
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     rep_origin      3563..3640
                     /vntifkey="33"
                     /label=SV40_origin
     promoter        3396..3664
                     /vntifkey="30"
                     /label=SV40_promoter
     misc_feature    complement(3384..3599)
                     /vntifkey="21"
                     /label=SV40_enhancer
     misc_feature    complement(3378..3398)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     rep_origin      2958..3264
                     /vntifkey="33"
                     /label=f1_origin
     terminator      2668..2895
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     misc_feature    complement(2665..2682)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     promoter        1546..1564
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    1477..1497
                     /vntifkey="21"
                     /label=EF1a_fwd_primer
     CDS             complement(649..1269)
                     /vntifkey="4"
                     /label=ORF\frame\1
     promoter        358..1529
                     /vntifkey="30"
                     /label=EF1a_promoter
     CDS             1679..2641
                     /vntifkey="4"
                     /label=BirA
     misc_feature    1640..1666
                     /vntifkey="21"
                     /label=flag\epitope
     CDS             3826..4221
                     /vntifkey="4"
                     /label=Blasticidin
BASE COUNT     1525 a      1649 c      1819 g      1705 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg 
     1621 tggtggaatt ctgcagatca tggattacaa ggacgatgac gataaggtac ccggatccat 
     1681 gaaggataac accgtgccac tgaaattgat tgccctgtta gcgaacggtg aatttcactc 
     1741 tggcgagcag ttgggtgaaa cgctgggaat gagccgggcg gctattaata aacacattca 
     1801 gacactgcgt gactggggcg ttgatgtctt taccgttccg ggtaaaggat acagcctgcc 
     1861 tgagcctatc cagttactta atgctaaaca gatattgggt cagctggatg gcggtagtgt 
     1921 agccgtgctg ccagtgattg actccacgaa tcagtacctt cttgatcgta tcggagagct 
     1981 taaatcgggc gatgcttgca ttgcagaata ccagcaggct ggccgtggtc gccggggtcg 
     2041 gaaatggttt tcgccttttg gcgcaaactt atatttgtcg atgttctggc gtctggaaca 
     2101 aggcccggcg gcggcgattg gtttaagtct ggttatcggt atcgtgatgg cggaagtatt 
     2161 acgcaagctg ggtgcagata aagttcgtgt taaatggcct aatgacctct atctgcagga 
     2221 tcgcaagctg gcaggcattc tggtggagct gactggcaaa actggcgatg cggcgcaaat 
     2281 agtcattgga gccgggatca acatggcaat gcgccgtgtt gaagagagtg tcgttaatca 
     2341 ggggtggatc acgctgcagg aagcggggat caatctcgat cgtaatacgt tggcggccat 
     2401 gctaatacgt gaattacgtg ctgcgttgga actcttcgaa caagaaggat tggcacctta 
     2461 tctgtcgcgc tgggaaaagc tggataattt tattaatcgc ccagtgaaac ttatcattgg 
     2521 tgataaagaa atatttggca tttcacgcgg aatagacaaa cagggggctt tattacttga 
     2581 gcaggatgga ataataaaac cctggatggg cggtgaaata tccctgcgta gtgcagaaaa 
     2641 ataagaatta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 
     2701 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 
     2761 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 
     2821 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 
     2881 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 
     2941 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 
     3001 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 
     3061 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 
     3121 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 
     3181 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 
     3241 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 
     3301 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 
     3361 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 
     3421 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 
     3481 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 
     3541 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     3601 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 
     3661 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 
     3721 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca 
     3781 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg 
     3841 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc 
     3901 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt 
     3961 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct 
     4021 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc 
     4081 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa 
     4141 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc 
     4201 tctggttatg tgtgggaggg ctaagcactt cgtggccgag gagcaggact gacacgtgct 
     4261 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 
     4321 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 
     4381 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 
     4441 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 
     4501 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct 
     4561 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 
     4621 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 
     4681 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 
     4741 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 
     4801 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     4861 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc aggaaccgta 
     4921 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
     4981 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
     5041 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
     5101 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 
     5161 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
     5221 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
     5281 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
     5341 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 
     5401 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
     5461 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     5521 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     5581 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
     5641 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 
     5701 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 
     5761 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 
     5821 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 
     5881 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 
     5941 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 
     6001 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 
     6061 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 
     6121 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 
     6181 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 
     6241 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 
     6301 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 
     6361 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 
     6421 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 
     6481 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 
     6541 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 
     6601 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 
     6661 ttccgcgcac atttccccga aaagtgccac ctgacgtc 
//
