LOCUS       pGEX-4T-3-mTctex1        5828 bp    DNA     circular     24-JAN-2011
DEFINITION  LMBP 6816.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6816
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 24-JAN-2011
            
            
            
            
COMMENT     VNTDATE|569955093|
COMMENT     VNTNAME|pGEX-4T-3-mTctex1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (CDocSetData 1 1 0 0 1 9 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 1 
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COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 931 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 7 1061 0 3672 0 4042 0 
COMMENT            4147 0 4928 0 5171 0 5562 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HincII" "gtyrac" 4 0 4 185 0 1095 0 2482 0 
COMMENT            5034 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 1820 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BstZI" "cggccg" 2 0 1 1820 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 939 0 0 "")) 
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COMMENT            1 1 1 1 "258..911") 
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COMMENT            (CStringList) (CStringList) 1 1 1 1 "918..935") 
COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 0 2236 3096 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2236..3096") 
COMMENT        #9=(CFSignal (CObList) "mTctex1" 4 0 0 978 1319 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "978..1319") 
COMMENT        #10=(CFSignal (CObList) "pMB1 ori" 33 0 0 3637 4039 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3637..4039") 
COMMENT        #11=(CFSignal (CObList) "tac promoter" 30 0 0 182 216 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 0 5344 5381 0 
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COMMENT        #13=(CFSignal (CObList) "lacIq promoter, incl. RBS" 30 0 0 4099 4176 0 
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COMMENT        #14=(CFSignal (CObList) "lacI" 4 0 0 4177 5259 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "lac promoter" 30 0 0 5260 5343 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "lacZ fragment, incl. lacZalpha" 4 0 0 5382 5822 
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COMMENT                          "Currently local object. Original author: Uta" 1) 
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COMMENT                          "Created: 11/04/10 04:51" 1) 
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COMMENT                          "Promoter Prokaryotic (3 total)") 
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COMMENT        #327=(CSampFrag "pGEX-4T-3-mTctex1" 4947 1820 "BstZI" 939 "EcoRI" 
COMMENT              (LOGPEN 0 0 0) 2.36937e-316 1.02894e-314) 
COMMENT        #328=(CSampFrag "pGEX-4T-3-mTctex1" 881 939 "EcoRI" 1820 "BstZI" 
COMMENT              (LOGPEN 0 0 0) 2.3695e-316 1.02894e-314))))
FEATURES             Location/Qualifiers
     CDS             258..911
                     /vntifkey="4"
                     /label=GST
     misc_feature    918..935
                     /vntifkey="21"
                     /label=thrombin\recognition\site
     CDS             2236..3096
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             978..1319
                     /vntifkey="4"
                     /label=mTctex1
     rep_origin      3637..4039
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        182..216
                     /vntifkey="30"
                     /label=tac\promoter
     RBS             5344..5381
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        4099..4176
                     /vntifkey="30"
                     /label=lacIq\promoter,\incl.\RBS
     CDS             4177..5259
                     /vntifkey="4"
                     /label=lacI
     promoter        5260..5343
                     /vntifkey="30"
                     /label=lac\promoter
     CDS             5382..5822
                     /vntifkey="4"
                     /label=lacZ\fragment,\incl.\lacZalpha
     misc_feature    1320..1817
                     /vntifkey="21"
                     /label=3'\UTR\of\mTctex1
BASE COUNT     1582 a      1386 c      1437 g      1423 t 
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 
      901 atcctccaaa atcggatctg gttccgcgtg gatccccgaa ttcggcacaa ttcggcacga 
      961 gaggagatgc gttaaagatg gaagacttcc aggcctcaga ggagactgca tttgttgttg 
     1021 atgaagtgag cagcattgta aaggaggcta tagaaagcgc catcggtggt aatgcctacc 
     1081 agcacagcaa agtcaaccag tggaccacta atgtcctaga acagactttg agccaactca 
     1141 ccaaactggg gagaccattt aaatacattg tgacctgtgt gatcatgcag aagaacggtg 
     1201 ctgggttaca ctccgcaagt tcctgcttct gggacagctc cacagacgga agctgcacag 
     1261 tccgatggga gaacaagacc atgtactgca tcgtcagtac cttcggactg tccatctgac 
     1321 caccttgcca gcctcagcct cgtggtccca gcatttccag tccatcttaa ccaccagcta 
     1381 tgttgggcaa accccttcct cctaagttgt tctgtggcac tctcaaaaaa ccaaatgact 
     1441 ccccagtcac aggaaccaca tgacttcagc cagattagca gccacctgtc atctgagcca 
     1501 ggcagtacca cttgtcttaa ctgtcttctc caaggtgcta aaaactcaag tctgctggtg 
     1561 gaaacagctc tactttctga aatgattcag atacactaac tttccatact ttattctttc 
     1621 ttagaataat aaattattca aaattaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 
     1681 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 
     1741 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 
     1801 aaactcgacc tcctagtgcg gccgcatcgt gactgactga cgatctgcct cgcgcgtttc 
     1861 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg 
     1921 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt 
     1981 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtataattc ttgaagacga 
     2041 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag 
     2101 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 
     2161 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 
     2221 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 
     2281 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 
     2341 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 
     2401 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 
     2461 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat 
     2521 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 
     2581 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 
     2641 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 
     2701 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 
     2761 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa 
     2821 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 
     2881 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 
     2941 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 
     3001 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 
     3061 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 
     3121 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 
     3181 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 
     3241 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 
     3301 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 
     3361 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 
     3421 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 
     3481 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 
     3541 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 
     3601 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 
     3661 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 
     3721 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 
     3781 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 
     3841 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 
     3901 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 
     3961 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 
     4021 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt 
     4081 tcacaccgca taaattccga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga 
     4141 tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat 
     4201 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 
     4261 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 
     4321 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 
     4381 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 
     4441 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 
     4501 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 
     4561 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 
     4621 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 
     4681 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 
     4741 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 
     4801 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 
     4861 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 
     4921 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 
     4981 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 
     5041 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 
     5101 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 
     5161 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 
     5221 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 
     5281 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 
     5341 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacggatt 
     5401 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 
     5461 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 
     5521 gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac 
     5581 cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg 
     5641 tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac gtaacctatc 
     5701 ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca 
     5761 catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg 
     5821 ttggaatt 
//
