LOCUS       pENTR1A-crmA            3766 bp    DNA     circular     26-MAY-2014
DEFINITION  LMBP 6824.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6824
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 26-MAY-2014
COMMENT     VNTDATE|699018785|
COMMENT     VNTNAME|pENTR1A-crmA|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
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COMMENT        #2=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 2643 0 0 "") 
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COMMENT        #6=(CFSignal (CObList) "5' UTR of cowpox virus crmA" 21 0 0 500 792 0 
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COMMENT                          "Currently local object. Original author: Marlies" 1) 
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COMMENT                          "Created: 05/26/14 11:53" 1) 
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FEATURES             Location/Qualifiers
     misc_feature    1819..1964
                     /vntifkey="21"
                     /label=3'\UTR\of\cowpox\virus\crmA
     misc_feature    500..792
                     /vntifkey="21"
                     /label=5'\UTR\of\cowpox\virus\crmA
     CDS             793..1818
                     /vntifkey="4"
                     /label=cowpox\virus\crmA
     CDS             2217..3026
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
     rep_origin      3486..45
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      281..308
                     /vntifkey="43"
                     /label=T2\terminator
     attenuator      359..454
                     /vntifkey="0"
                     /label=attL1
     attenuator      complement(1998..2093)
                     /vntifkey="0"
                     /label=attL2
     terminator      complement(106..149)
                     /vntifkey="43"
                     /label=T1\terminator
BASE COUNT     1089 a       745 c       850 g      1082 t 
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac 
      481 tggatccggt accgaattcc catggaagaa cgaaagtagt ataaaagtaa taaaacaaaa 
      541 aaaagaatat aaaaaattta tagccacttt ctttgaggac tgttttcctg aaggaaatga 
      601 acctctggaa ttagttagat atatagaatt agtatacacg ctagattatt ctcaaactcc 
      661 taattatgac agactacgta gactgtttat acaagattga aaatatattt ctttttattg 
      721 agtggtggta gttacggata tctaatatta atattagact atctctatcg tcacacaaca 
      781 aaatcgattg ccatggatat cttcagggaa atcgcatctt ctatgaaagg agagaatgta 
      841 ttcatttctc caccgtcaat ctcgtcagta ttgacaatac tgtattatgg agctaatgga 
      901 tccactgctg aacagctatc aaaatatgta gaaaaggagg cggacaagaa taaggatgat 
      961 atctcattca agtccatgaa taaagtatat gggcgatatt ctgcagtgtt taaagattcc 
     1021 tttttgagaa aaattggaga taatttccaa actgttgact tcactgattg tcgcactgta 
     1081 gatgcgatca acaagtgtgt tgatatcttc actgagggga aaattaatcc actattggat 
     1141 gaaccattgt ctccagatac ctgtctccta gcaattagtg ccgtatactt taaagcaaaa 
     1201 tggttgatgc catttgaaaa ggaatttacc agtgattatc ccttttacgt atctccaacg 
     1261 gaaatggtag atgtaagtat gatgtctatg tacggcgagg catttaatca cgcatctgta 
     1321 aaagaatcat tcggcaactt ttcaatcata gaactgccat atgttggaga tactagtatg 
     1381 gtggtaattc ttccagacaa tattgatgga ctagaatcca tagaacaaaa tctaacagat 
     1441 acaaatttta agaaatggtg tgactctatg gatgctatgt ttatcgatgt gcacattccc 
     1501 aagtttaagg taacaggctc gtataatctg gtggatgcgc tagtaaagtt gggactgaca 
     1561 gaggtgttcg gttcaactgg agattatagc aatatgtgta attcagatgt gagtgtcgac 
     1621 gctatgatcc acaaaacgta tatagatgtc aatgaagagt atacagaagc agctgcagca 
     1681 acttgtgcgc tggtggcaga ctgtgcatca acagttacaa atgagttctg tgcagatcat 
     1741 ccgttcatct atgtgattag gcatgtcgat ggcaaaattc ttttcgttgg tagatattgc 
     1801 tctccaacaa ctaattaaat cacattctta atattagaat attagaatat tatatagtta 
     1861 agatttttac taattggtta accatttttt taaaaaaata gaaaaaaaac atgttatatt 
     1921 agcgagggtc gttattcttc caattgcaat tggtaagatg acggggaatt cgcggccgca 
     1981 ctcgagatat ctagacccag ctttcttgta caaagttggc attataagaa agcattgctt 
     2041 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc 
     2101 agctgcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata 
     2161 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga 
     2221 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat 
     2281 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt 
     2341 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg 
     2401 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca 
     2461 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa 
     2521 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc 
     2581 tggcagtgtc cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg 
     2641 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga 
     2701 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata 
     2761 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc 
     2821 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag 
     2881 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac 
     2941 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc 
     3001 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cattattcag 
     3061 attgggcccc gttccactga gcgtcagacc cggtagaaaa gatcaaagga tcttcttgag 
     3121 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 
     3181 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 
     3241 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 
     3301 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 
     3361 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 
     3421 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 
     3481 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 
     3541 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 
     3601 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 
     3661 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 
     3721 cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt 
//
