LOCUS       pTOPTK-iGFP             7515 bp    DNA     circular      2-MAR-2011
DEFINITION  LMBP 6847.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6847
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 2-MAR-2011
            
COMMENT     VNTDATE|583592115|
COMMENT     VNTNAME|pTOPTK-iGFP|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pTOPTK-iGFP" 0 7515 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BstXI" "SacI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7515 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
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COMMENT            (CStringList) 1 1 1 1 "467..1124") 
COMMENT        #1=(CFSignal (CObList) "SP6 promoter" 30 0 0 411 427 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "411..427") 
COMMENT        #2=(CFSignal (CObList) "CS2 insertion" 14 0 0 430 439 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "430..439") 
COMMENT        #3=(CFSignal (CObList) "B-globin 5' mRNA seq" 52 0 0 440 454 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "440..454") 
COMMENT        #4=(CFSignal (CObList) "T7 promoter" 30 0 1 1176 1192 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1176..1192)") 
COMMENT        #5=(CFSignal (CObList) "E-cadh promotor" 29 0 0 34 378 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "34..378") 
COMMENT        #6=(CFSignal (CObList) "14XUAS" 21 0 1 6069 6440 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6069..6440)") 
COMMENT        #7=(CFSignal (CObList) "E1b basal promoter" 30 0 0 6445 6504 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "6445..6504") 
COMMENT        #8=(CFSignal (CObList) "EGFP" 4 0 0 6504 7261 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "6504..7268") 
COMMENT        #9=(CFSignal (CObList) "TOP" 21 0 0 4077 4086 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "TOP" 21 0 0 4092 4101 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "TOP" 21 0 0 4107 4116 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "HSV-TK promoter" 29 0 0 4155 4316 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4155..4316") 
COMMENT        #13=(CFSignal (CObList) "GAL4VP16GR" 4 0 0 4357 5835 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4357..5835") 
COMMENT        #14=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2612 3472 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2615..3472") 
COMMENT        #15=(CFSignal (CObList) "T7 promoter" 30 0 1 5834 5850 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5834..5850)") 
COMMENT        #16=(CFSignal (CObList) "T7 promoter" 30 0 1 7282 7298 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7282..7298)") 
COMMENT        #17=(CFSignal (CObList) "SV40 polyA late" 43 0 0 7363 7459 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "SV40 polyA late" 43 0 0 1257 1353 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "SV40 polyA early" 43 0 1 1257 1353 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #20=(CFSignal (CObList) "SV40 polyA early" 43 0 1 5915 6011 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "SV40 polyA late" 43 0 0 5915 6011 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #22=(CFSignal (CObList) "SV40 polyA early" 43 0 1 7363 7459 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #23=(CFSignal (CObList) "GALbd" 4 0 0 4357 4797 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
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COMMENT        #26=(CFSignal (CObList) "LMBP 6847" 2 0 0 0 0 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 03/02/11 12:55" 1) 
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COMMENT                          "Last Modified: 03/02/11 12:55" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7515 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 6-OCT-2010" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                           "Terminator (6 total)") 
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FEATURES             Location/Qualifiers
     CDS             467..1124
                     /vntifkey="4"
                     /label=DsRED
     promoter        411..427
                     /vntifkey="30"
                     /label=SP6\promoter
     insertion_seq   430..439
                     /vntifkey="14"
                     /label=CS2\insertion
     5'UTR           440..454
                     /vntifkey="52"
                     /label=B-globin\5'\mRNA\seq
     promoter        complement(1176..1192)
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        34..378
                     /vntifkey="29"
                     /label=E-cadh\promotor
     misc_feature    complement(6069..6440)
                     /vntifkey="21"
                     /label=14XUAS
     promoter        6445..6504
                     /vntifkey="30"
                     /label=E1b\basal\promoter
     CDS             6504..7261
                     /vntifkey="4"
                     /label=EGFP
     misc_feature    4077..4086
                     /vntifkey="21"
                     /label=TOP
     misc_feature    4092..4101
                     /vntifkey="21"
                     /label=TOP
     misc_feature    4107..4116
                     /vntifkey="21"
                     /label=TOP
     promoter        4155..4316
                     /vntifkey="29"
                     /label=HSV-TK\promoter
     CDS             4357..5835
                     /vntifkey="4"
                     /label=GAL4VP16GR
                     /note="ORF: Frame #1   Start: atg (924)   Stop:  tag (2402)"
     CDS             complement(2612..3472)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        complement(5834..5850)
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(7282..7298)
                     /vntifkey="30"
                     /label=T7\promoter
     terminator      7363..7459
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      1257..1353
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      complement(1257..1353)
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(5915..6011)
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      5915..6011
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      complement(7363..7459)
                     /vntifkey="43"
                     /label=SV40\polyA\early
     CDS             4357..4797
                     /vntifkey="4"
                     /label=GALbd
     rep_origin      complement(1669..2071)
                     /vntifkey="33"
                     /label=pMB1\ori
BASE COUNT     1907 a      1942 c      1958 g      1708 t 
ORIGIN
        1 cgataggtac cgagctctta cgcgtgctag cccacaaaag aactcagcca agtgtaaaag 
       61 ccctttctga tcccaggtct tagtgagcca ccggcggggc tgggattcga acccagtgga 
      121 atcagaaccg tgcaggtccc ataacccacc tagaccctag caactccagg ctagagggtc 
      181 accgcgtcta tgcgaggccg ggtgggcggg ccgtcagctc cgccctgggg aggggtccgc 
      241 gctgctgatt ggctgtggcc ggcaggtgaa ccctcagcca atcagcggta cggggggcgg 
      301 tgctccgggg ctcacctggc tgcagccacg caccccctct cagtggcgtc ggaactgcaa 
      361 agcacctgtg agcttgcggg ggctcgagat ctgcgatcta agtaagcttg atttaggtga 
      421 cactatagaa tacaagctac ttgttctttt tgcaggatcc accggtcgcc accatggcct 
      481 cctccgagga cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga 
      541 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga 
      601 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc 
      661 cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc cccgactaca 
      721 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 
      781 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg ctccttcatc tacaaggtga 
      841 agttcatcgg cgtgaacttc ccctccgacg gccccgtaat gcagaagaag actatgggct 
      901 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccaca 
      961 aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagtcc atctacatgg 
     1021 ccaagaagcc cgtgcagctg cccggctact actacgtgga ctccaagctg gacatcacct 
     1081 cccacaacga ggactacacc atcgtggagc agtacgagcg cgccgagggc cgccaccacc 
     1141 tgttcctgta gcggcccctc tcgagcctct agaactatag tgagtcgtat tacgtagatc 
     1201 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa 
     1261 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca 
     1321 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt 
     1381 gggaggtttt ttaattcgcg gccgcggcgc caatgcattg atcggtaccg agctcgaatt 
     1441 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 
     1501 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 
     1561 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 
     1621 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 
     1681 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 
     1741 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 
     1801 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 
     1861 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 
     1921 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 
     1981 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 
     2041 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 
     2101 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 
     2161 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 
     2221 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 
     2281 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 
     2341 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 
     2401 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 
     2461 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 
     2521 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 
     2581 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 
     2641 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 
     2701 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 
     2761 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 
     2821 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 
     2881 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 
     2941 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 
     3001 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 
     3061 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 
     3121 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 
     3181 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 
     3241 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 
     3301 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 
     3361 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 
     3421 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 
     3481 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 
     3541 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 
     3601 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga 
     3661 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 
     3721 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 
     3781 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg 
     3841 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 
     3901 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 
     3961 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 
     4021 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctaagat 
     4081 caaagggggt aagatcaaag ggggtaagat caaagggagc ttgcatgcct gcaggtcgac 
     4141 tctagaggat ccggccccgc ccagcgtctt gtcattggcg aattcgaaca cgcagatgca 
     4201 gtcggggcgg cgcggtccga ggtccacttc gcatattaag gtgacgcgtg tggcctcgaa 
     4261 caccgagcga ccctgcagcg acccgcttaa cagcgtcaac agcgtgccgc agatctaagc 
     4321 tcgaattcaa ggagcttgaa gcaagcctcc tgaaagatga agctactgtc ttctatcgaa 
     4381 caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc 
     4441 gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg 
     4501 ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt 
     4561 ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc tttacaggat 
     4621 ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga tgccgtcaca 
     4681 gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca tagaataagt 
     4741 gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac tgtatcgtcg 
     4801 aggtcgaccc cgggaattca gatctctcga gccgcccccc cgaccgatgt cagcctgggg 
     4861 gacgagctcc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat 
     4921 ttcgatctgg acatgttggg ggacggggat tccccgggtc cgggatttac cccccacgac 
     4981 tccgccccct acggcgctct ggatatggca ggttcgctag ctgaaaatcc tggtaacaaa 
     5041 acaatagttc ctgcaacgtt accacaactc acccctaccc tggtgtcact gttggaggtt 
     5101 attgaacctg aagtgttata tgcaggatat gatagctctg ttccagactc aacttggagg 
     5161 atcatgacta cgctcaacat gttaggaggg cggcaagtga ttgcagcagt gaaatgggca 
     5221 aaggcaatac caggtttcag gaacttacac ctggatgacc aaatgaccct actgcagtac 
     5281 tcctggatgt ttcttatggc atttgctctg gggtggagat catatagaca atcaagtgca 
     5341 aacctgctgt gttttgctcc tgatctgatt attaatgagc agagaatgac tctaccctgc 
     5401 atgtacgacc aatgtaaaca catgctgtat gtttcctctg agttacacag gcttcaggta 
     5461 tcttatgaag agtatctctg tatgaaaacc ttactgcttc tctcttcagt tcctaaggac 
     5521 ggtctgaaga gccaagagct atttgatgaa attagaatga cctacatcaa agagctagga 
     5581 aaagccattg tcaagaggga aggaaactcc agccagaact ggcagcggtt ttatcaactg 
     5641 acaaaactct tggattctat gcatgaagtg gttgaaaatc tccttaacta ttgcttccaa 
     5701 acatttttgg ataagaccat gagtattgaa ttccccgaga tgttagctga aatcatcacc 
     5761 aatcagatac caaaatattc aaatggaaat atcaaaaaac ttctgtttca tcaaaagctc 
     5821 gagcctctag aactatagtg agtcgtatta cgtagatcca gacatgataa gatacattga 
     5881 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg 
     5941 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa 
     6001 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aattcgccat 
     6061 ggtcaagctt aggcctccaa ggcggagtac tgtcctccgg gctggcggag tactgtcctc 
     6121 cggcaaggtc ggagtactgt cctccgacac tagaggtcgg agtactgtcc tccgacgcaa 
     6181 ggcggagtac tgtcctccgg gctgcggagt actgtcctcc ggcaaggtcg gagtactgtc 
     6241 ctccgacact agaggtcgga gtactgtcct ccgacgcaag gtcggagtac tgtcctccga 
     6301 cactagaggt cggagtactg tcctccgacg caaggtcgga gtactgtcct ccgacactag 
     6361 aggtcggagt actgtcctcc gacgcaaggc ggagtactgt cctccgggct ggcggagtac 
     6421 tgtcctccgg caagggtcga ctctagaggg tatataatgg atcccatcgc gtctcagcct 
     6481 cactttgagc tcctccacac gaattccctc gacctcgaaa ttcaaggagc ttgccgccac 
     6541 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 
     6601 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 
     6661 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 
     6721 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa 
     6781 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 
     6841 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 
     6901 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 
     6961 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 
     7021 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 
     7081 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 
     7141 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 
     7201 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta 
     7261 aagcggccgc gactctagaa ctatagtgag tcgtattacg tagatccaga catgataaga 
     7321 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt 
     7381 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac 
     7441 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa 
     7501 ttcgcggccg cggat 
//
