LOCUS       pEF6-hsa-miR19a+20a+19b.1+92a.1        6644 bp    DNA     circular     10-MAY-2009
DEFINITION  LMBP 6892.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|517957715|
COMMENT     VNTNAME|pEF6-hsa-miR19a+20a+19b.1+92a.1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-hsa-miR19a+20a+19b.1+92a.1" 0 6644 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 1931477113 0 "" 0 "" "" "" "" "" "" "pEF6MycHisA" 
COMMENT             "" 0 0 1 5910 1 5910 1751 1727 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "EcoRI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 1931477113 0 "" 0 "" "" "" "" "" "" 
COMMENT             "hsa-miR-19a+20a+19b.1+92a.1" "" 0 0 1 734 5911 6644 3 736 0 "" "" 
COMMENT             "" "" "" "" (CObList) (CTerm 1 0 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "EcoRI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 1 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AatII" "Acc65I" "AccI" "AflII" "AflIII" "AgeI" "AluI" 
COMMENT         "AlwNI" "ApaI" "ApaLI" "AspI" "AvaI" "AvrII" "BamHI" "BanI" "BbsI" 
COMMENT         "BclI" "BcnI" "BglI" "BglII" "BslI" "Bsp1286I" "BspEI" "BspHI" "BsrBI" 
COMMENT         "BsrGI" "Bst1107I" "Bst98I" "BstEII" "BstNI" "BstXI" "BstYI" "Bsu36I" 
COMMENT         "ClaI" "Csp45I" "DdeI" "DpnI" "DraI" "DraIII" "DrdI" "EaeI" "EagI" 
COMMENT         "Eam1105I" "EarI" "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "Esp3I" "FokI" 
COMMENT         "FspI" "HaeII" "HincII" "HindIII" "HpaI" "HphI" "KasI" "KpnI" "Ksp632I" 
COMMENT         "KspI" "MaeIII" "MamI" "MboI" "MfeI" "MluI" "MnlI" "MscI" "MseI" "MslI" 
COMMENT         "NaeI" "NcoI" "NdeI" "NheI" "NotI" "NruI" "NsiI" "Nsp(7524)I" "NspI" 
COMMENT         "PacI" "PflMI" "PmeI" "PpiI" "PstI" "PvuI" "PvuII" "RsaI" "SacI" 
COMMENT         "SacII" "SalI" "SapI" "Sau3AI" "ScaI" "SfiI" "SinI" "SmaI" "SnaBI" 
COMMENT         "SpeI" "SphI" "SspI" "SstI" "SstII" "StuI" "StyI" "TaqI" "Tth111I" 
COMMENT         "XbaI" "XhoI" "XmaI" "XmnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6644 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #2=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 4183 0 4305 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 5899 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AccI" "gtmkac" 3 0 4 1988 0 1995 0 4305 0 4339 
COMMENT            0 0 "") #5=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 5038 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "AflIII" "acrygt" 2 0 5 1231 0 1727 0 2368 0 
COMMENT            4534 0 5292 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AgeI" "accggt" 2 0 2 94 0 4737 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "AluI" "agct" 3 0 37 392 0 559 0 816 0 1136 0 
COMMENT            1190 0 1200 0 1403 0 1508 0 1872 0 2005 0 2011 0 2033 0 2128 0 2192 
COMMENT            0 2310 0 2536 0 2672 0 2929 0 3450 0 3550 0 3613 0 4441 0 4573 0 
COMMENT            4619 0 4997 0 5355 0 5485 0 5629 0 5721 0 5845 0 5882 0 5895 0 5907 
COMMENT            0 6128 0 6207 0 6221 0 6297 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 3 2784 0 4386 0 6131 0 
COMMENT            0 "") 
COMMENT        #10=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 50 0 5276 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 2 2682 0 3928 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AspI" "gacnnngtc" 5 0 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 34 0 1193 0 4704 0 4806 
COMMENT             0 4992 0 5507 0 5624 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 1172 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 5911 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 13 211 0 605 0 1575 0 2112 
COMMENT             0 3209 0 4665 0 4740 0 5113 0 5372 0 5432 0 5597 0 5608 0 5899 0 0 
COMMENT             "") 
COMMENT        #17=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 335 0 1386 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 133 0 1223 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "BcnI" "cccgg" 3 4 10 1194 0 1195 1 1794 1 
COMMENT             2748 0 3444 1 3795 0 5426 0 5467 0 5595 1 6443 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 4 3381 0 5430 0 5606 
COMMENT             0 5723 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "BglII" "agatct" 2 0 2 4318 0 5225 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 24 427 0 753 0 1080 0 
COMMENT             1572 0 1823 0 2216 0 2390 0 2408 0 2574 0 2853 0 4675 0 4676 0 4750 
COMMENT             0 4812 0 4813 0 4928 0 4999 0 5080 0 5334 0 5407 0 5408 0 5466 0 
COMMENT             5478 0 5539 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 14 50 0 364 0 400 0 
COMMENT             1490 0 1565 0 2686 0 3847 0 3932 0 4670 0 5048 0 5276 0 5487 0 5909 
COMMENT             0 6191 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 3088 0 4096 0 4218 0 0 
COMMENT             "") 
COMMENT        #26=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 8 33 0 502 0 2060 0 2301 
COMMENT             1 4102 1 4224 1 5448 1 5516 1 0 "") 
COMMENT        #27=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 6527 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "Bst1107I" "gtatac" 4 0 1 1989 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "Bst98I" "cttaag" 2 0 1 5038 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 17 205 0 886 0 941 0 958 0 
COMMENT             1605 0 2108 0 2396 0 2517 0 2530 0 4918 0 4967 0 5075 0 5080 0 5377 
COMMENT             0 5408 0 5606 0 6108 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 23 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 13 78 0 1218 0 1826 0 
COMMENT             3009 0 3020 0 3106 0 3118 0 3886 0 3903 0 4318 0 5225 0 5769 0 5911 
COMMENT             0 0 "") #34=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "Csp45I" "ttcgaa" 3 0 1 53 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "DdeI" "ctnag" 2 0 15 69 0 265 0 374 0 1132 0 
COMMENT             1696 0 2643 0 3052 0 3218 0 3758 0 4345 0 4415 0 4486 0 5561 0 5692 
COMMENT             0 5791 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "DpnI" "gatc" 3 0 30 80 0 135 0 1220 0 1225 0 
COMMENT             1535 0 1596 0 1610 0 1812 0 1828 0 2936 0 3011 0 3022 0 3030 0 3108 
COMMENT             0 3120 0 3225 0 3566 0 3584 0 3630 0 3888 0 3905 0 3941 0 4312 0 
COMMENT             4320 0 4328 0 4990 0 5227 0 5771 0 5913 0 6401 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "DraI" "tttaaa" 4 0 6 124 0 3127 0 3146 0 3838 
COMMENT             0 4597 0 5160 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 3 649 0 1706 0 5695 0 
COMMENT             0 "") 
COMMENT        #41=(CRSite (CStringList) "DrdI" "gacnnnnnngtc" 8 0 3 693 0 1388 0 2476 
COMMENT             0 0 "") 
COMMENT        #42=(CRSite (CStringList) "EaeI" "yggccr" 2 0 9 28 0 1302 0 1417 0 1708 
COMMENT             0 2207 0 3649 0 5322 0 5357 0 5457 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "EagI" "cggccg" 2 0 2 28 0 1417 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "Eam1105I" "gacnnnnngtc" 7 0 1 3261 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "EarI" "ctcttc" 8 11 3 68 1 2252 0 4056 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 2 5485 0 5907 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 6 46 0 47 0 1460 0 
COMMENT             5272 0 5309 0 5548 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 13 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "Esp3I" "cgtctcn" 8 12 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "FokI" "ggatg" 15 19 9 364 0 1023 1 1352 1 
COMMENT             1587 1 1820 0 3227 1 3408 1 3695 1 6277 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 3483 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 7 491 0 499 0 2246 0 2616 
COMMENT             0 5087 0 5507 0 5601 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "HincII" "gtyrac" 4 0 5 1237 0 1996 0 4306 0 
COMMENT             4340 0 4540 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 4571 0 5893 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "HpaI" "gttaac" 4 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "HphI" "ggtga" 14 13 11 97 1 103 1 646 0 1297 
COMMENT             0 3104 1 3331 1 3747 0 3953 1 3988 0 5521 1 5535 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "KasI" "ggcgcc" 2 0 1 5597 0 0 "") 
COMMENT        #59=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 5903 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "Ksp632I" "ctcttcn" 8 11 3 68 1 2252 0 4056 0 
COMMENT             0 "") 
COMMENT        #61=(CRSite (CStringList) "KspI" "ccgcgg" 5 0 2 4913 0 5260 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "MaeIII" "gtnac" 1 0 17 456 0 468 0 1881 0 
COMMENT             2724 0 2787 0 2903 0 3186 0 3517 0 3575 0 3728 0 3916 0 5527 0 5577 
COMMENT             0 6121 0 6251 0 6468 0 6559 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "MamI" "gatnnnnatc" 6 0 1 1599 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "MboI" "gatc" 1 0 30 78 0 133 0 1218 0 1223 0 
COMMENT             1533 0 1594 0 1608 0 1810 0 1826 0 2934 0 3009 0 3020 0 3028 0 3106 
COMMENT             0 3118 0 3223 0 3564 0 3582 0 3628 0 3886 0 3903 0 3939 0 4310 0 
COMMENT             4318 0 4326 0 4988 0 5225 0 5769 0 5911 0 6399 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "MfeI" "caattg" 2 0 2 4467 0 4730 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 4534 0 0 "") 
COMMENT        #67=(CRSite (CStringList) "MnlI" "cctc" 12 11 50 37 1 69 1 150 0 192 0 
COMMENT             237 1 312 1 369 1 619 0 1103 1 1109 1 1133 0 1139 0 1146 1 1149 1 
COMMENT             1161 1 1281 1 1343 0 1684 1 1684 0 1706 1 1824 0 2009 0 2217 1 2267 
COMMENT             0 2476 1 2550 0 2800 1 3200 1 3281 1 3428 0 3634 0 4401 1 4598 1 
COMMENT             4650 1 4661 0 4713 1 4802 1 4932 0 5020 1 5063 1 5065 0 5296 1 5391 
COMMENT             0 5488 1 5570 0 5622 0 5654 1 5800 0 5829 1 5975 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "MscI" "tggcca" 4 0 1 1304 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "MseI" "ttaa" 2 0 28 123 0 436 0 707 0 805 0 
COMMENT             822 0 833 0 843 0 1243 0 2139 0 2198 0 3074 0 3126 0 3131 0 3145 0 
COMMENT             3198 0 3433 0 3472 0 3837 0 4375 0 4437 0 4596 0 5039 0 5159 0 5944 
COMMENT             0 6193 0 6490 0 6522 0 6547 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "MslI" "caynnnnrtg" 6 0 4 3513 0 3672 0 4031 0 
COMMENT             6142 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "NaeI" "gccggc" 4 0 3 543 0 1802 0 5361 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 1079 0 1299 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 6203 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 28 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "NruI" "tcgcga" 4 0 2 1532 0 4514 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "NsiI" "atgcat" 6 0 3 92 0 922 0 994 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "Nsp(7524)I" "rcatgy" 6 0 5 346 0 920 0 992 0 
COMMENT             2372 0 5296 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "NspI" "rcatgy" 6 0 5 346 0 920 0 992 0 2372 0 
COMMENT             5296 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "PflMI" "ccannnnntgg" 8 0 0 0 "") 
COMMENT        #82=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 1 124 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "PpiI" "gaacnnnnnctc" 26 21 4 79 0 685 1 3083 
COMMENT             0 3893 1 0 "") 
COMMENT        #84=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 10 0 4975 0 5480 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 1536 0 3631 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "PvuII" "cagctg" 4 0 4 392 0 1508 0 2192 0 
COMMENT             6297 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "RsaI" "gtac" 3 0 12 3741 0 4350 0 4520 0 4788 
COMMENT             0 4977 0 5592 0 5639 0 5851 0 5901 0 6079 0 6199 0 6529 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "SacI" "gagctc" 6 0 2 5487 0 5909 0 0 "") 
COMMENT        #89=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 4913 0 5260 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "SalI" "gtcgac" 2 0 3 1994 0 4304 0 4338 0 0 
COMMENT             "") #91=(CRSite (CStringList) "SapI" "gctcttc" 9 12 1 2252 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Sau3AI" "gatc" 1 0 30 78 0 133 0 1218 0 1223 
COMMENT             0 1533 0 1594 0 1608 0 1810 0 1826 0 2934 0 3009 0 3020 0 3028 0 
COMMENT             3106 0 3118 0 3223 0 3564 0 3582 0 3628 0 3886 0 3903 0 3939 0 4310 
COMMENT             0 4318 0 4326 0 4988 0 5225 0 5769 0 5911 0 6399 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "ScaI" "agtact" 4 0 2 3741 0 6199 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "SinI" "ggwcc" 2 0 4 1460 0 3399 0 3621 0 4625 
COMMENT             0 0 "") 
COMMENT        #96=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 1195 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "SphI" "gcatgc" 6 0 3 346 0 920 0 992 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "SspI" "aatatt" 4 0 4 4065 0 4187 0 6495 0 
COMMENT              6545 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "SstI" "gagctc" 6 0 2 5487 0 5909 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "SstII" "ccgcgg" 5 0 2 4913 0 5260 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "StuI" "aggcct" 4 0 3 1171 0 5030 0 5073 0 0 
COMMENT              "") 
COMMENT        #104=(CRSite (CStringList) "StyI" "ccwwgg" 2 0 4 1079 0 1172 0 1299 0 
COMMENT              5743 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "TaqI" "tcga" 2 0 17 35 0 53 0 142 0 611 0 
COMMENT              1593 0 1744 0 1995 0 2468 0 3912 0 4305 0 4339 0 5025 0 5140 0 
COMMENT              5614 0 5625 0 6092 0 6509 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "Tth111I" "gacnnngtc" 5 0 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 40 0 0 "") 
COMMENT        #108=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 34 0 5624 0 0 "") 
COMMENT        #109=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 1193 0 0 "") 
COMMENT        #110=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 3 844 0 3860 0 4958 0 
COMMENT              0 "")) 
COMMENT       (CObList 
COMMENT        #111=(CFSignal (CObList) "Amp" 4 0 0 3187 4047 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #112=(CFSignal (CObList) "colE1" 33 0 0 2369 3042 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #113=(CFSignal (CObList) "bla" 4 0 0 1300 1698 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #114=(CFSignal (CObList) "EM-7" 29 0 0 1226 1281 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #115=(CFSignal (CObList) "SV40" 29 0 0 870 1178 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #116=(CFSignal (CObList) "f1" 33 0 0 415 843 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #117=(CFSignal (CObList) "BGH polyA" 25 0 0 142 369 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #118=(CFSignal (CObList) "His" 21 0 0 99 116 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #119=(CFSignal (CObList) "myc" 21 0 0 54 83 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #120=(CFSignal (CObList) "T7" 30 0 0 5853 5872 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #121=(CFSignal (CObList) "EF-1alpha" 29 0 0 4653 5836 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #122=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT              (CObjectList 
COMMENT               #123=(CRefLinePar 
COMMENT                     (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) 
COMMENT                      "pEF6-hsa-miR19a+20a+19b.1+92a.1" 2) 5 "" 0 4) 
COMMENT               #124=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #125=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "DNA 'pEF6-hsa-miR19a+20a+19b.1+92a.1'" 1) 
COMMENT                       #126=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Local object" 1) 
COMMENT                       #127=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Created: 05/10/09 09:08" 1) 
COMMENT                       #128=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Last Modified: 05/10/09 09:08" 1) 
COMMENT                       #129=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "length: 6644 bp" 1) 
COMMENT                       #130=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "storage type: Constructed" 1) 
COMMENT                       #131=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "form: Circular" 1))) "General Description") 
COMMENT               #132=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) 
COMMENT                     "Standard Fields") 
COMMENT               #133=(CRefLinePar 
COMMENT                     (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 
COMMENT                     "" 0 0) 
COMMENT               #134=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                     "Annotations") 
COMMENT               #135=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 10 (0 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #136=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (5 #0# 0) 1 2 1 0 195) 
COMMENT                              (CObjectList 
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FEATURES             Location/Qualifiers
     CDS             3187..4047
                     /vntifkey="4"
                     /label=Amp
     rep_origin      2369..3042
                     /vntifkey="33"
                     /label=colE1
     CDS             1300..1698
                     /vntifkey="4"
                     /label=bla
     promoter        1226..1281
                     /vntifkey="29"
                     /label=EM-7
     promoter        870..1178
                     /vntifkey="29"
                     /label=SV40
     rep_origin      415..843
                     /vntifkey="33"
                     /label=f1
     polyA_signal    142..369
                     /vntifkey="25"
                     /label=BGH\polyA
     misc_feature    99..116
                     /vntifkey="21"
                     /label=His
     misc_feature    54..83
                     /vntifkey="21"
                     /label=myc
     promoter        5853..5872
                     /vntifkey="30"
                     /label=T7
     promoter        4653..5836
                     /vntifkey="29"
                     /label=EF-1alpha
BASE COUNT     1529 a      1623 c      1726 g      1766 t 
ORIGIN
        1 aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc ttcgaacaaa 
       61 aactcatctc agaagaggat ctgaatatgc ataccggtca tcatcaccat caccattgag 
      121 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 
      181 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 
      241 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 
      301 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 
      361 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc 
      421 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 
      481 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 
      541 ccggctttcc ccgtcaagct ctaaatcggg gcgtcccttt agggttccga tttagtgctt 
      601 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 
      661 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 
      721 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 
      781 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 
      841 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 
      901 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 
      961 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 
     1021 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 
     1081 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 
     1141 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 
     1201 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat 
     1261 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagcc tttgtctcaa 
     1321 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa 
     1381 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat 
     1441 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct 
     1501 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg 
     1561 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata 
     1621 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt 
     1681 tatgtgtggg agggctaagc acttcgtggc cgaggagcag gactgacacg tgctacgaga 
     1741 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 
     1801 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 
     1861 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
     1921 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 
     1981 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 
     2041 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     2101 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     2161 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 
     2221 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     2281 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     2341 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     2401 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     2461 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     2521 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     2581 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta 
     2641 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     2701 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     2761 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     2821 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 
     2881 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     2941 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
     3001 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
     3061 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 
     3121 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 
     3181 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 
     3241 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 
     3301 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 
     3361 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 
     3421 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 
     3481 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 
     3541 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 
     3601 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 
     3661 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 
     3721 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 
     3781 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 
     3841 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 
     3901 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 
     3961 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 
     4021 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 
     4081 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 
     4141 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcaatatta ttgaagcatt 
     4201 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 
     4261 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat 
     4321 ctcccgatcc cctatggtcg actctcagta caatctgctc tgatgccgca tagttaagcc 
     4381 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag 
     4441 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt 
     4501 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactaggc 
     4561 ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat ctttgcagct 
     4621 aatggacctt ctaggtcttg aaaggagtgc ctcgtgaggc tccggtgccc gtcagtgggc 
     4681 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg 
     4741 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 
     4801 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 
     4861 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg 
     4921 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt 
     4981 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta 
     5041 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt 
     5101 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta 
     5161 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg 
     5221 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc 
     5281 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg 
     5341 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc 
     5401 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc 
     5461 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc 
     5521 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga 
     5581 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac 
     5641 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt 
     5701 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt 
     5761 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc 
     5821 atttcaggtg tcgtgaggaa ttagcttggt actaatacga ctcactatag ggagacccaa 
     5881 gctggctagg taagcttggt accgagctcg gatccttcaa gccaagcaag tatataggtg 
     5941 ttttaatagt ttttgtttgc agtcctctgt tagttttgca tagttgcact acaagaagaa 
     6001 tgtagttgtg caaatctatg caaaactgat ggtggcctgc tatttccttc aaatgaatga 
     6061 tttttactaa ttttgtgtac ttttattgtg tcgatgtaga atctgcctgg tctatctgat 
     6121 gtgacagctt ctgtagcact aaagtgctta tagtgcaggt agtgtttagt tatctactgc 
     6181 attatgagca cttaaagtac tgctagctgt agaactccag cttcggcctg tcgcccaatc 
     6241 aaactgtcct gttactgaac actgttctat ggttagtttt gcaggtttgc atccagctgt 
     6301 gtgatattct gctgtgcaaa tccatgcaaa actgactgtg gtagtgaaaa gtctgtagaa 
     6361 aagtaaggga aactcaaacc cctttctaca caggttggga tcggttgcaa tgctgtgttt 
     6421 ctgtatggta ttgcacttgt cccggcctgt tgagtttggt ggggattgtg accagaagat 
     6481 tttgaaaatt aaatattact gaagatttcg acttccactg ttaaatgtac aagatacatg 
     6541 aaatattaaa gaaaatgtgt aactttttgt gtaaatacat cttgtcttgt tttcattcaa 
     6601 aaacatttca cttttggggt tgcgtgtcag atttggcagt atag 
//
