LOCUS       pEF6-hsa-miR30b        6265 bp    DNA     circular     10-MAY-2009
DEFINITION  LMBP 6894.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|517957597|
COMMENT     VNTNAME|pEF6-hsa-miR30b|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-hsa-miR30b" 0 6265 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 1931477113 0 "" 0 "" "" "" "" "" "" "pEF6MycHisA" 
COMMENT             "" 0 0 1 5910 1 5910 1751 1727 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "EcoRI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 1931477113 0 "" 0 "" "" "" "" "" "" "hsa-miR-30b" 
COMMENT             "" 0 0 1 351 5911 6261 3 353 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "EcoRI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 1 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AatII" "Acc65I" "AccI" "AflII" "AflIII" "AgeI" "AluI" 
COMMENT         "AlwNI" "ApaI" "ApaLI" "AspI" "AvaI" "AvrII" "BamHI" "BanI" "BbsI" 
COMMENT         "BclI" "BcnI" "BglI" "BglII" "BslI" "Bsp1286I" "BspEI" "BspHI" "BsrBI" 
COMMENT         "BsrGI" "Bst1107I" "Bst98I" "BstEII" "BstNI" "BstXI" "BstYI" "Bsu36I" 
COMMENT         "ClaI" "Csp45I" "DdeI" "DpnI" "DraI" "DraIII" "DrdI" "EaeI" "EagI" 
COMMENT         "Eam1105I" "EarI" "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "Esp3I" "FokI" 
COMMENT         "FspI" "HaeII" "HincII" "HindIII" "HpaI" "HphI" "KasI" "KpnI" "Ksp632I" 
COMMENT         "KspI" "MaeIII" "MamI" "MboI" "MfeI" "MluI" "MnlI" "MscI" "MseI" "MslI" 
COMMENT         "NaeI" "NcoI" "NdeI" "NheI" "NotI" "NruI" "NsiI" "Nsp(7524)I" "NspI" 
COMMENT         "PacI" "PflMI" "PmeI" "PpiI" "PstI" "PvuI" "PvuII" "RsaI" "SacI" 
COMMENT         "SacII" "SalI" "SapI" "Sau3AI" "ScaI" "SfiI" "SinI" "SmaI" "SnaBI" 
COMMENT         "SpeI" "SphI" "SspI" "SstI" "SstII" "StuI" "StyI" "TaqI" "Tth111I" 
COMMENT         "XbaI" "XhoI" "XmaI" "XmnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6265 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #2=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 4187 0 4309 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 5903 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AccI" "gtmkac" 3 0 5 1992 0 1999 0 4309 0 4343 
COMMENT            0 6155 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 5042 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "AflIII" "acrygt" 2 0 5 1235 0 1731 0 2372 0 
COMMENT            4538 0 5296 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AgeI" "accggt" 2 0 2 98 0 4741 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "AluI" "agct" 3 0 36 396 0 563 0 820 0 1140 0 
COMMENT            1194 0 1204 0 1407 0 1512 0 1876 0 2009 0 2015 0 2037 0 2132 0 2196 
COMMENT            0 2314 0 2540 0 2676 0 2933 0 3454 0 3554 0 3617 0 4445 0 4577 0 
COMMENT            4623 0 5001 0 5359 0 5489 0 5633 0 5725 0 5849 0 5886 0 5899 0 5911 
COMMENT            0 6060 0 6102 0 6257 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 2 2788 0 4390 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 50 0 5280 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 2 2686 0 3932 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AspI" "gacnnngtc" 5 0 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 34 0 1197 0 4708 0 4810 
COMMENT             0 4996 0 5511 0 5628 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 1176 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 5915 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 13 215 0 609 0 1579 0 2116 
COMMENT             0 3213 0 4669 0 4744 0 5117 0 5376 0 5436 0 5601 0 5612 0 5903 0 0 
COMMENT             "") 
COMMENT        #17=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 339 0 1390 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 137 0 1227 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "BcnI" "cccgg" 3 4 9 1198 0 1199 1 1798 1 2752 
COMMENT             0 3448 1 3799 0 5430 0 5471 0 5599 1 0 "") 
COMMENT        #20=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 4 3385 0 5434 0 5610 
COMMENT             0 5727 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "BglII" "agatct" 2 0 2 4322 0 5229 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 24 431 0 757 0 1084 0 
COMMENT             1576 0 1827 0 2220 0 2394 0 2412 0 2578 0 2857 0 4679 0 4680 0 4754 
COMMENT             0 4816 0 4817 0 4932 0 5003 0 5084 0 5338 0 5411 0 5412 0 5470 0 
COMMENT             5482 0 5543 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 13 50 0 368 0 404 0 
COMMENT             1494 0 1569 0 2690 0 3851 0 3936 0 4674 0 5052 0 5280 0 5491 0 5913 
COMMENT             0 0 "") #24=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 3092 0 4100 0 4222 0 0 
COMMENT             "") 
COMMENT        #26=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 8 33 0 506 0 2064 0 2305 
COMMENT             1 4106 1 4228 1 5452 1 5520 1 0 "") 
COMMENT        #27=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "Bst1107I" "gtatac" 4 0 2 1993 0 6156 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "Bst98I" "cttaag" 2 0 1 5042 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 16 209 0 890 0 945 0 962 0 
COMMENT             1609 0 2112 0 2400 0 2521 0 2534 0 4922 0 4971 0 5079 0 5084 0 5381 
COMMENT             0 5412 0 5610 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 23 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 13 82 0 1222 0 1830 0 
COMMENT             3013 0 3024 0 3110 0 3122 0 3890 0 3907 0 4322 0 5229 0 5773 0 5915 
COMMENT             0 0 "") #34=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "Csp45I" "ttcgaa" 3 0 1 57 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "DdeI" "ctnag" 2 0 17 73 0 269 0 378 0 1136 0 
COMMENT             1700 0 2647 0 3056 0 3222 0 3762 0 4349 0 4419 0 4490 0 5565 0 5696 
COMMENT             0 5795 0 5996 0 6056 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "DpnI" "gatc" 3 0 29 84 0 139 0 1224 0 1229 0 
COMMENT             1539 0 1600 0 1614 0 1816 0 1832 0 2940 0 3015 0 3026 0 3034 0 3112 
COMMENT             0 3124 0 3229 0 3570 0 3588 0 3634 0 3892 0 3909 0 3945 0 4316 0 
COMMENT             4324 0 4332 0 4994 0 5231 0 5775 0 5917 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "DraI" "tttaaa" 4 0 7 128 0 3131 0 3150 0 3842 
COMMENT             0 4601 0 5164 0 6022 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 3 653 0 1710 0 5699 0 
COMMENT             0 "") 
COMMENT        #41=(CRSite (CStringList) "DrdI" "gacnnnnnngtc" 8 0 3 697 0 1392 0 2480 
COMMENT             0 0 "") 
COMMENT        #42=(CRSite (CStringList) "EaeI" "yggccr" 2 0 9 28 0 1306 0 1421 0 1712 
COMMENT             0 2211 0 3653 0 5326 0 5361 0 5461 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "EagI" "cggccg" 2 0 2 28 0 1421 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "Eam1105I" "gacnnnnngtc" 7 0 1 3265 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "EarI" "ctcttc" 8 11 3 72 1 2256 0 4060 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 2 5489 0 5911 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 5 46 0 1464 0 5276 0 
COMMENT             5313 0 5552 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 13 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "Esp3I" "cgtctcn" 8 12 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "FokI" "ggatg" 15 19 10 368 0 1027 1 1356 1 
COMMENT             1591 1 1824 0 3231 1 3412 1 3699 1 6033 1 6101 0 0 "") 
COMMENT        #52=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 3487 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 7 495 0 503 0 2250 0 2620 
COMMENT             0 5091 0 5511 0 5605 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "HincII" "gtyrac" 4 0 6 1241 0 2000 0 4310 0 
COMMENT             4344 0 4544 0 6117 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 4575 0 5897 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "HpaI" "gttaac" 4 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "HphI" "ggtga" 14 13 11 101 1 107 1 650 0 1301 
COMMENT             0 3108 1 3335 1 3751 0 3957 1 3992 0 5525 1 5539 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "KasI" "ggcgcc" 2 0 1 5601 0 0 "") 
COMMENT        #59=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 5907 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "Ksp632I" "ctcttcn" 8 11 3 72 1 2256 0 4060 0 
COMMENT             0 "") 
COMMENT        #61=(CRSite (CStringList) "KspI" "ccgcgg" 5 0 3 53 0 4917 0 5264 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "MaeIII" "gtnac" 1 0 13 460 0 472 0 1885 0 
COMMENT             2728 0 2791 0 2907 0 3190 0 3521 0 3579 0 3732 0 3920 0 5531 0 5581 
COMMENT             0 0 "") 
COMMENT        #63=(CRSite (CStringList) "MamI" "gatnnnnatc" 6 0 1 1603 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "MboI" "gatc" 1 0 29 82 0 137 0 1222 0 1227 0 
COMMENT             1537 0 1598 0 1612 0 1814 0 1830 0 2938 0 3013 0 3024 0 3032 0 3110 
COMMENT             0 3122 0 3227 0 3568 0 3586 0 3632 0 3890 0 3907 0 3943 0 4314 0 
COMMENT             4322 0 4330 0 4992 0 5229 0 5773 0 5915 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "MfeI" "caattg" 2 0 2 4471 0 4734 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 4538 0 0 "") 
COMMENT        #67=(CRSite (CStringList) "MnlI" "cctc" 12 11 52 37 1 73 1 154 0 196 0 
COMMENT             241 1 316 1 373 1 623 0 1107 1 1113 1 1137 0 1143 0 1150 1 1153 1 
COMMENT             1165 1 1285 1 1347 0 1688 1 1688 0 1710 1 1828 0 2013 0 2221 1 2271 
COMMENT             0 2480 1 2554 0 2804 1 3204 1 3285 1 3432 0 3638 0 4405 1 4602 1 
COMMENT             4654 1 4665 0 4717 1 4806 1 4936 0 5024 1 5067 1 5069 0 5300 1 5395 
COMMENT             0 5492 1 5574 0 5626 0 5658 1 5804 0 5833 1 5939 0 6076 1 6154 1 0 
COMMENT             "") #68=(CRSite (CStringList) "MscI" "tggcca" 4 0 1 1308 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "MseI" "ttaa" 2 0 30 127 0 440 0 711 0 809 0 
COMMENT             826 0 837 0 847 0 1247 0 2143 0 2202 0 3078 0 3130 0 3135 0 3149 0 
COMMENT             3202 0 3437 0 3476 0 3841 0 4379 0 4441 0 4600 0 5043 0 5163 0 6009 
COMMENT             0 6021 0 6124 0 6174 0 6183 0 6192 0 6246 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "MslI" "caynnnnrtg" 6 0 4 3517 0 3676 0 4035 0 
COMMENT             5936 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "NaeI" "gccggc" 4 0 3 547 0 1806 0 5365 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 1083 0 1303 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 28 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "NruI" "tcgcga" 4 0 2 1536 0 4518 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "NsiI" "atgcat" 6 0 3 96 0 926 0 998 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "Nsp(7524)I" "rcatgy" 6 0 5 350 0 924 0 996 0 
COMMENT             2376 0 5300 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "NspI" "rcatgy" 6 0 5 350 0 924 0 996 0 2376 0 
COMMENT             5300 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "PflMI" "ccannnnntgg" 8 0 0 0 "") 
COMMENT        #82=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 1 128 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "PpiI" "gaacnnnnnctc" 26 21 4 83 0 689 1 3087 
COMMENT             0 3897 1 0 "") 
COMMENT        #84=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 10 0 4979 0 5484 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 1540 0 3635 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "PvuII" "cagctg" 4 0 5 396 0 1512 0 2196 0 
COMMENT             6060 0 6102 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "RsaI" "gtac" 3 0 10 3745 0 4354 0 4524 0 4792 
COMMENT             0 4981 0 5596 0 5643 0 5855 0 5905 0 5990 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "SacI" "gagctc" 6 0 2 5491 0 5913 0 0 "") 
COMMENT        #89=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 3 53 0 4917 0 5264 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "SalI" "gtcgac" 2 0 3 1998 0 4308 0 4342 0 0 
COMMENT             "") #91=(CRSite (CStringList) "SapI" "gctcttc" 9 12 1 2256 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Sau3AI" "gatc" 1 0 29 82 0 137 0 1222 0 1227 
COMMENT             0 1537 0 1598 0 1612 0 1814 0 1830 0 2938 0 3013 0 3024 0 3032 0 
COMMENT             3110 0 3122 0 3227 0 3568 0 3586 0 3632 0 3890 0 3907 0 3943 0 4314 
COMMENT             0 4322 0 4330 0 4992 0 5229 0 5773 0 5915 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 3745 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "SinI" "ggwcc" 2 0 4 1464 0 3403 0 3625 0 4629 
COMMENT             0 0 "") 
COMMENT        #96=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 1199 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 1 5978 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "SphI" "gcatgc" 6 0 3 350 0 924 0 996 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "SspI" "aatatt" 4 0 2 4069 0 4191 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "SstI" "gagctc" 6 0 2 5491 0 5913 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "SstII" "ccgcgg" 5 0 3 53 0 4917 0 5264 0 0 
COMMENT              "") 
COMMENT        #103=(CRSite (CStringList) "StuI" "aggcct" 4 0 3 1175 0 5034 0 5077 0 0 
COMMENT              "") 
COMMENT        #104=(CRSite (CStringList) "StyI" "ccwwgg" 2 0 4 1083 0 1176 0 1303 0 
COMMENT              5747 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "TaqI" "tcga" 2 0 15 35 0 57 0 146 0 615 0 
COMMENT              1597 0 1748 0 1999 0 2472 0 3916 0 4309 0 4343 0 5029 0 5144 0 
COMMENT              5618 0 5629 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "Tth111I" "gacnnngtc" 5 0 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 40 0 0 "") 
COMMENT        #108=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 34 0 5628 0 0 "") 
COMMENT        #109=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 1197 0 0 "") 
COMMENT        #110=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 3 848 0 3864 0 4962 0 
COMMENT              0 "")) 
COMMENT       (CObList 
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COMMENT        #115=(CFSignal (CObList) "SV40" 29 0 0 874 1182 1 (CStringList) 
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COMMENT        #116=(CFSignal (CObList) "f1" 33 0 0 419 847 1 (CStringList) 
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COMMENT        #117=(CFSignal (CObList) "BGH polyA" 25 0 0 146 373 1 (CStringList) 
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COMMENT                             "Created: 05/10/09 09:06" 1) 
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COMMENT                             "Last Modified: 05/10/09 09:06" 1) 
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COMMENT                             "length: 6265 bp" 1) 
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FEATURES             Location/Qualifiers
     CDS             3191..4051
                     /vntifkey="4"
                     /label=Amp
     rep_origin      2373..3046
                     /vntifkey="33"
                     /label=colE1
     CDS             1304..1702
                     /vntifkey="4"
                     /label=bla
     promoter        1230..1285
                     /vntifkey="29"
                     /label=EM-7
     promoter        874..1182
                     /vntifkey="29"
                     /label=SV40
     rep_origin      419..847
                     /vntifkey="33"
                     /label=f1
     polyA_signal    146..373
                     /vntifkey="25"
                     /label=BGH\polyA
     misc_feature    103..120
                     /vntifkey="21"
                     /label=His
     misc_feature    58..87
                     /vntifkey="21"
                     /label=myc
     promoter        5857..5876
                     /vntifkey="30"
                     /label=T7
     promoter        4657..5840
                     /vntifkey="29"
                     /label=EF-1alpha
BASE COUNT     1441 a      1556 c      1645 g      1623 t 
ORIGIN
        1 aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa 
       61 caaaaactca tctcagaaga ggatctgaat atgcataccg gtcatcatca ccatcaccat 
      121 tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 
      181 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 
      241 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 
      301 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 
      361 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 
      421 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 
      481 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 
      541 ttcgccggct ttccccgtca agctctaaat cggggcgtcc ctttagggtt ccgatttagt 
      601 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 
      661 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 
      721 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 
      781 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 
      841 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 
      901 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 
      961 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 
     1021 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     1081 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 
     1141 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 
     1201 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata 
     1261 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agcctttgtc 
     1321 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc 
     1381 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt 
     1441 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc 
     1501 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat 
     1561 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc 
     1621 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc 
     1681 tggttatgtg tgggagggct aagcacttcg tggccgagga gcaggactga cacgtgctac 
     1741 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 
     1801 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 
     1861 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 
     1921 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 
     1981 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 
     2041 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 
     2101 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
     2161 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 
     2221 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 
     2281 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
     2341 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     2401 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     2461 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     2521 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     2581 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta 
     2641 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     2701 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     2761 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     2821 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 
     2881 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     2941 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     3001 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     3061 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     3121 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
     3181 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
     3241 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
     3301 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
     3361 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
     3421 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
     3481 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
     3541 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
     3601 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
     3661 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
     3721 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
     3781 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
     3841 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
     3901 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
     3961 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
     4021 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
     4081 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
     4141 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat attattgaag 
     4201 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
     4261 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg 
     4321 agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc cgcatagtta 
     4381 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt 
     4441 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg 
     4501 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact 
     4561 aggcttttgc aaaaagcttt gcaaagatgg ataaagtttt aaacagagag gaatctttgc 
     4621 agctaatgga ccttctaggt cttgaaagga gtgcctcgtg aggctccggt gcccgtcagt 
     4681 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa 
     4741 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc 
     4801 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc 
     4861 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc 
     4921 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt 
     4981 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg 
     5041 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc 
     5101 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca 
     5161 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg 
     5221 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc 
     5281 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg 
     5341 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta 
     5401 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat 
     5461 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc 
     5521 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat 
     5581 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga 
     5641 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt 
     5701 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc 
     5761 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc 
     5821 ttccatttca ggtgtcgtga ggaattagct tggtactaat acgactcact atagggagac 
     5881 ccaagctggc taggtaagct tggtaccgag ctcggatccg agtcttgcct catttatgtg 
     5941 aatgctgtgc ctgttctttt tttcaacaga gtcttacgta aagaaccgta caaacttagt 
     6001 aaagagttta agtcctgctt taaaccaagt ttcagttcat gtaaacatcc tacactcagc 
     6061 tgtaatacat ggattggctg ggaggtggat gtttacttca gctgacttgg aatgtcaacc 
     6121 aattaacatt gataaaagat ttggcaagaa tagtatacag aggcttgaat ttttaatgta 
     6181 attaatgtaa ttaaaggttt gttggaaatg tgagaccatt ttgttctccc agagaaaaag 
     6241 tgtgttaatt gtctagctgg caggg 
//
