LOCUS       pEF6-hsa-miR182        6361 bp    DNA     circular     10-MAY-2009
DEFINITION  LMBP 6903.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|517957268|
COMMENT     VNTNAME|pEF6-hsa-miR182|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-hsa-miR182" 0 6361 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 1931477113 0 "" 0 "" "" "" "" "" "" "hsa-miR-182" 
COMMENT             "" 0 0 1 459 1 459 7 465 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "KpnI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "EcoRI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 1931477113 0 "" 0 "" "" "" "" "" "" "pEF6MycHisA" 
COMMENT             "" 0 0 1 5902 460 6361 1751 1719 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "EcoRI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "KpnI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 1 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AatII" "Acc65I" "AccI" "AflII" "AflIII" "AgeI" "AluI" 
COMMENT         "AlwNI" "ApaI" "ApaLI" "AspI" "AvaI" "AvrII" "BamHI" "BanI" "BbsI" 
COMMENT         "BclI" "BcnI" "BglI" "BglII" "BslI" "Bsp1286I" "BspEI" "BspHI" "BsrBI" 
COMMENT         "BsrGI" "Bst1107I" "Bst98I" "BstEII" "BstNI" "BstXI" "BstYI" "Bsu36I" 
COMMENT         "ClaI" "Csp45I" "DdeI" "DpnI" "DraI" "DraIII" "DrdI" "EaeI" "EagI" 
COMMENT         "Eam1105I" "EarI" "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "Esp3I" "FokI" 
COMMENT         "FspI" "HaeII" "HincII" "HindIII" "HpaI" "HphI" "KasI" "KpnI" "Ksp632I" 
COMMENT         "KspI" "MaeIII" "MamI" "MboI" "MfeI" "MluI" "MnlI" "MscI" "MseI" "MslI" 
COMMENT         "NaeI" "NcoI" "NdeI" "NheI" "NotI" "NruI" "NsiI" "Nsp(7524)I" "NspI" 
COMMENT         "PacI" "PflMI" "PmeI" "PpiI" "PstI" "PvuI" "PvuII" "RsaI" "SacI" 
COMMENT         "SacII" "SalI" "SapI" "Sau3AI" "ScaI" "SfiI" "SinI" "SmaI" "SnaBI" 
COMMENT         "SpeI" "SphI" "SspI" "SstI" "SstII" "StuI" "StyI" "TaqI" "Tth111I" 
COMMENT         "XbaI" "XhoI" "XmaI" "XmnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6361 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #2=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 4642 0 4764 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 6358 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AccI" "gtmkac" 3 0 4 2447 0 2454 0 4764 0 4798 
COMMENT            0 0 "") #5=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 5497 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "AflIII" "acrygt" 2 0 5 1690 0 2186 0 2827 0 
COMMENT            4993 0 5751 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AgeI" "accggt" 2 0 2 553 0 5196 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "AluI" "agct" 3 0 34 239 0 252 0 851 0 1018 0 
COMMENT            1275 0 1595 0 1649 0 1659 0 1862 0 1967 0 2331 0 2464 0 2470 0 2492 
COMMENT            0 2587 0 2651 0 2769 0 2995 0 3131 0 3388 0 3909 0 4009 0 4072 0 
COMMENT            4900 0 5032 0 5078 0 5456 0 5814 0 5944 0 6088 0 6180 0 6304 0 6341 
COMMENT            0 6354 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 5 20 0 242 0 436 0 3243 
COMMENT            0 4845 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 509 0 5735 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 363 0 3141 0 4387 0 0 
COMMENT             "") #12=(CRSite (CStringList) "AspI" "gacnnngtc" 5 0 1 141 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 493 0 1652 0 5163 0 5265 
COMMENT             0 5451 0 5966 0 6083 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 1631 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 308 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 15 162 0 355 0 670 0 1064 
COMMENT             0 2034 0 2571 0 3668 0 5124 0 5199 0 5572 0 5831 0 5891 0 6056 0 
COMMENT             6067 0 6358 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 794 0 1845 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 592 0 1682 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "BcnI" "cccgg" 3 4 10 75 1 1653 0 1654 1 2253 
COMMENT             1 3207 0 3903 1 4254 0 5885 0 5926 0 6054 1 0 "") 
COMMENT        #20=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 4 3840 0 5889 0 6065 
COMMENT             0 6182 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "BglII" "agatct" 2 0 2 4777 0 5684 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 28 118 0 242 0 272 0 
COMMENT             336 0 886 0 1212 0 1539 0 2031 0 2282 0 2675 0 2849 0 2867 0 3033 0 
COMMENT             3312 0 5134 0 5135 0 5209 0 5271 0 5272 0 5387 0 5458 0 5539 0 5793 
COMMENT             0 5866 0 5867 0 5925 0 5937 0 5998 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 13 367 0 509 0 823 0 
COMMENT             859 0 1949 0 2024 0 3145 0 4306 0 4391 0 5129 0 5507 0 5735 0 5946 
COMMENT             0 0 "") #24=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 3547 0 4555 0 4677 0 0 
COMMENT             "") 
COMMENT        #26=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 8 492 0 961 0 2519 0 2760 
COMMENT             1 4561 1 4683 1 5907 1 5975 1 0 "") 
COMMENT        #27=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "Bst1107I" "gtatac" 4 0 1 2448 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "Bst98I" "cttaag" 2 0 1 5497 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 21 118 0 174 0 184 0 243 0 
COMMENT             437 0 664 0 1345 0 1400 0 1417 0 2064 0 2567 0 2855 0 2976 0 2989 0 
COMMENT             5377 0 5426 0 5534 0 5539 0 5836 0 5867 0 6065 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 482 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 13 308 0 537 0 1677 0 
COMMENT             2285 0 3468 0 3479 0 3565 0 3577 0 4345 0 4362 0 4777 0 5684 0 6228 
COMMENT             0 0 "") #34=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "Csp45I" "ttcgaa" 3 0 1 512 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "DdeI" "ctnag" 2 0 19 63 0 214 0 348 0 429 0 
COMMENT             528 0 724 0 833 0 1591 0 2155 0 3102 0 3511 0 3677 0 4217 0 4804 0 
COMMENT             4874 0 4945 0 6020 0 6151 0 6250 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "DpnI" "gatc" 3 0 29 310 0 539 0 594 0 1679 0 
COMMENT             1684 0 1994 0 2055 0 2069 0 2271 0 2287 0 3395 0 3470 0 3481 0 3489 
COMMENT             0 3567 0 3579 0 3684 0 4025 0 4043 0 4089 0 4347 0 4364 0 4400 0 
COMMENT             4771 0 4779 0 4787 0 5449 0 5686 0 6230 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "DraI" "tttaaa" 4 0 6 583 0 3586 0 3605 0 4297 
COMMENT             0 5056 0 5619 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 4 362 0 1108 0 2165 0 
COMMENT             6154 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "DrdI" "gacnnnnnngtc" 8 0 3 1152 0 1847 0 2935 
COMMENT             0 0 "") 
COMMENT        #42=(CRSite (CStringList) "EaeI" "yggccr" 2 0 10 387 0 487 0 1761 0 1876 
COMMENT             0 2167 0 2666 0 4108 0 5781 0 5816 0 5916 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "EagI" "cggccg" 2 0 2 487 0 1876 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "Eam1105I" "gacnnnnngtc" 7 0 1 3720 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "EarI" "ctcttc" 8 11 4 214 0 527 1 2711 0 4515 
COMMENT             0 0 "") 
COMMENT        #46=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 1 5944 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 8 137 0 433 0 505 0 
COMMENT             506 0 1919 0 5731 0 5768 0 6007 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 460 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 472 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "Esp3I" "cgtctcn" 8 12 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "FokI" "ggatg" 15 19 10 170 0 176 1 823 0 1482 
COMMENT             1 1811 1 2046 1 2279 0 3686 1 3867 1 4154 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 3942 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 8 166 0 950 0 958 0 2705 
COMMENT             0 3075 0 5546 0 5966 0 6060 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "HincII" "gtyrac" 4 0 5 1696 0 2455 0 4765 0 
COMMENT             4799 0 4999 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 5030 0 6352 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "HpaI" "gttaac" 4 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "HphI" "ggtga" 14 13 12 308 0 556 1 562 1 1105 
COMMENT             0 1756 0 3563 1 3790 1 4206 0 4412 1 4447 0 5980 1 5994 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "KasI" "ggcgcc" 2 0 2 162 0 6056 0 0 "") 
COMMENT        #59=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "Ksp632I" "ctcttcn" 8 11 4 214 0 527 1 2711 0 
COMMENT             4515 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "KspI" "ccgcgg" 5 0 2 5372 0 5719 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "MaeIII" "gtnac" 1 0 15 26 0 301 0 915 0 927 0 
COMMENT             2340 0 3183 0 3246 0 3362 0 3645 0 3976 0 4034 0 4187 0 4375 0 5986 
COMMENT             0 6036 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "MamI" "gatnnnnatc" 6 0 1 2058 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "MboI" "gatc" 1 0 29 308 0 537 0 592 0 1677 0 
COMMENT             1682 0 1992 0 2053 0 2067 0 2269 0 2285 0 3393 0 3468 0 3479 0 3487 
COMMENT             0 3565 0 3577 0 3682 0 4023 0 4041 0 4087 0 4345 0 4362 0 4398 0 
COMMENT             4769 0 4777 0 4785 0 5447 0 5684 0 6228 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "MfeI" "caattg" 2 0 2 4926 0 5189 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 4993 0 0 "") 
COMMENT        #67=(CRSite (CStringList) "MnlI" "cctc" 12 11 66 31 1 41 1 46 1 49 1 58 
COMMENT             1 61 1 119 0 176 0 220 1 223 0 241 0 270 0 292 1 336 1 369 1 408 1 
COMMENT             415 1 496 1 528 1 609 0 651 0 696 1 771 1 828 1 1078 0 1562 1 1568 
COMMENT             1 1592 0 1598 0 1605 1 1608 1 1620 1 1740 1 1802 0 2143 1 2143 0 
COMMENT             2165 1 2283 0 2468 0 2676 1 2726 0 2935 1 3009 0 3259 1 3659 1 3740 
COMMENT             1 3887 0 4093 0 4860 1 5057 1 5109 1 5120 0 5172 1 5261 1 5391 0 
COMMENT             5479 1 5522 1 5524 0 5755 1 5850 0 5947 1 6029 0 6081 0 6113 1 6259 
COMMENT             0 6288 1 0 "") 
COMMENT        #68=(CRSite (CStringList) "MscI" "tggcca" 4 0 2 389 0 1763 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "MseI" "ttaa" 2 0 23 582 0 895 0 1166 0 1264 0 
COMMENT             1281 0 1292 0 1302 0 1702 0 2598 0 2657 0 3533 0 3585 0 3590 0 3604 
COMMENT             0 3657 0 3892 0 3931 0 4296 0 4834 0 4896 0 5055 0 5498 0 5618 0 0 
COMMENT             "") 
COMMENT        #70=(CRSite (CStringList) "MslI" "caynnnnrtg" 6 0 4 294 0 3972 0 4131 0 
COMMENT             4490 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "NaeI" "gccggc" 4 0 4 354 0 1002 0 2261 0 5820 
COMMENT             0 0 "") 
COMMENT        #72=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 1538 0 1758 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 487 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "NruI" "tcgcga" 4 0 2 1991 0 4973 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "NsiI" "atgcat" 6 0 3 551 0 1381 0 1453 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "Nsp(7524)I" "rcatgy" 6 0 5 805 0 1379 0 1451 
COMMENT             0 2831 0 5755 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "NspI" "rcatgy" 6 0 5 805 0 1379 0 1451 0 2831 
COMMENT             0 5755 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "PflMI" "ccannnnntgg" 8 0 1 242 0 0 "") 
COMMENT        #82=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 1 583 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "PpiI" "gaacnnnnnctc" 26 21 4 538 0 1144 1 
COMMENT             3542 0 4352 1 0 "") 
COMMENT        #84=(CRSite (CStringList) "PstI" "ctgcag" 6 0 5 25 0 132 0 469 0 5434 0 
COMMENT             5939 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 1995 0 4090 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 851 0 1967 0 2651 0 0 
COMMENT             "") 
COMMENT        #87=(CRSite (CStringList) "RsaI" "gtac" 3 0 9 4200 0 4809 0 4979 0 5247 
COMMENT             0 5436 0 6051 0 6098 0 6310 0 6360 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 5946 0 0 "") 
COMMENT        #89=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 5372 0 5719 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "SalI" "gtcgac" 2 0 3 2453 0 4763 0 4797 0 0 
COMMENT             "") #91=(CRSite (CStringList) "SapI" "gctcttc" 9 12 1 2711 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Sau3AI" "gatc" 1 0 29 308 0 537 0 592 0 1677 
COMMENT             0 1682 0 1992 0 2053 0 2067 0 2269 0 2285 0 3393 0 3468 0 3479 0 
COMMENT             3487 0 3565 0 3577 0 3682 0 4023 0 4041 0 4087 0 4345 0 4362 0 4398 
COMMENT             0 4769 0 4777 0 4785 0 5447 0 5684 0 6228 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 4200 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "SinI" "ggwcc" 2 0 10 71 0 77 0 137 0 186 0 
COMMENT             397 0 433 0 1919 0 3858 0 4080 0 5084 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 1654 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "SphI" "gcatgc" 6 0 3 805 0 1379 0 1451 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "SspI" "aatatt" 4 0 2 4524 0 4646 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "SstI" "gagctc" 6 0 1 5946 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "SstII" "ccgcgg" 5 0 2 5372 0 5719 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "StuI" "aggcct" 4 0 4 230 0 1630 0 5489 0 
COMMENT              5532 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "StyI" "ccwwgg" 2 0 4 1538 0 1631 0 1758 0 
COMMENT              6202 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "TaqI" "tcga" 2 0 15 494 0 512 0 601 0 1070 0 
COMMENT              2052 0 2203 0 2454 0 2927 0 4371 0 4764 0 4798 0 5484 0 5599 0 
COMMENT              6073 0 6084 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "Tth111I" "gacnnngtc" 5 0 1 141 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 320 0 499 0 0 "") 
COMMENT        #108=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 493 0 6083 0 0 "") 
COMMENT        #109=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 1652 0 0 "") 
COMMENT        #110=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 3 1303 0 4319 0 5417 
COMMENT              0 0 "")) 
COMMENT       (CObList 
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COMMENT        #113=(CFSignal (CObList) "bla" 4 0 0 1759 2157 1 (CStringList) 
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COMMENT        #114=(CFSignal (CObList) "EM-7" 29 0 0 1685 1740 1 (CStringList) 
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COMMENT        #115=(CFSignal (CObList) "SV40" 29 0 0 1329 1637 1 (CStringList) 
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COMMENT        #116=(CFSignal (CObList) "f1" 33 0 0 874 1302 1 (CStringList) 
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COMMENT                             "Created: 05/10/09 09:01" 1) 
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COMMENT                             "Last Modified: 05/10/09 09:01" 1) 
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FEATURES             Location/Qualifiers
     CDS             3646..4506
                     /vntifkey="4"
                     /label=Amp
     rep_origin      2828..3501
                     /vntifkey="33"
                     /label=colE1
     CDS             1759..2157
                     /vntifkey="4"
                     /label=bla
     promoter        1685..1740
                     /vntifkey="29"
                     /label=EM-7
     promoter        1329..1637
                     /vntifkey="29"
                     /label=SV40
     rep_origin      874..1302
                     /vntifkey="33"
                     /label=f1
     polyA_signal    601..828
                     /vntifkey="25"
                     /label=BGH\polyA
     misc_feature    558..575
                     /vntifkey="21"
                     /label=His
     misc_feature    513..542
                     /vntifkey="21"
                     /label=myc
     promoter        6312..6331
                     /vntifkey="30"
                     /label=T7
     promoter        5112..6295
                     /vntifkey="29"
                     /label=EF-1alpha
BASE COUNT     1421 a      1624 c      1729 g      1587 t 
ORIGIN
        1 ccatgcctgc ccacaggaac tgcaggttac agatatgagg ggaagggagg agaggagggg 
       61 gctgaggagg gaccgggacc agcaggaagg gggactgtgg ggttgggcct ccacaccagg 
      121 gcgaccctgc aggaaggacc ttgtcgcagt tgcggggatg ggcgcctctg tcctggccct 
      181 gcctggacca tcctaactgt ctctgtctct tcctcagcac agaccgaggc ctccccagct 
      241 cctgggggga gctgcttgcc tccccccgtt tttggcaatg gtagaactca cactggtgag 
      301 gtaacaggat ccggtggttc tagacttgcc aactatgggg cgaggactca gccggcaccc 
      361 tgtgcacagc cagcgaggga agggctggcc atgctggacc tgctgttctc cgcgaggaag 
      421 gaggggactc aggtcctgga ctgctgggta gtggcagaga attctgcaga tatccagcac 
      481 agtggcggcc gctcgagtct agagggccct tcgaacaaaa actcatctca gaagaggatc 
      541 tgaatatgca taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct 
      601 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 
      661 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 
      721 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 
      781 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 
      841 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 
      901 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 
      961 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 
     1021 taaatcgggg cgtcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 
     1081 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 
     1141 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 
     1201 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 
     1261 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 
     1321 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 
     1381 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 
     1441 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 
     1501 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 
     1561 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt 
     1621 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc 
     1681 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac 
     1741 aaggtgagga actaaaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa 
     1801 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca 
     1861 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga 
     1921 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact 
     1981 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga 
     2041 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag 
     2101 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagca 
     2161 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt 
     2221 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 
     2281 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg 
     2341 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 
     2401 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc 
     2461 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 
     2521 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 
     2581 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 
     2641 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 
     2701 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 
     2761 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 
     2821 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 
     2881 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 
     2941 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 
     3001 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 
     3061 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 
     3121 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 
     3181 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 
     3241 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 
     3301 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 
     3361 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 
     3421 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 
     3481 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 
     3541 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 
     3601 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 
     3661 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 
     3721 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 
     3781 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 
     3841 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 
     3901 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 
     3961 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 
     4021 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 
     4081 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 
     4141 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 
     4201 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 
     4261 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 
     4321 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 
     4381 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 
     4441 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 
     4501 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 
     4561 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 
     4621 ccgaaaagtg ccacctgacg tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     4681 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     4741 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtcga 
     4801 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg 
     4861 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga 
     4921 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac 
     4981 gggccagata tacgcgttga cattgattat tgactaggct tttgcaaaaa gctttgcaaa 
     5041 gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga 
     5101 aaggagtgcc tcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 
     5161 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg 
     5221 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 
     5281 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 
     5341 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc 
     5401 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg 
     5461 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc 
     5521 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg 
     5581 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc 
     5641 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat 
     5701 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc 
     5761 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg 
     5821 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct 
     5881 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg 
     5941 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag 
     6001 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc 
     6061 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga 
     6121 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc 
     6181 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat 
     6241 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggaat 
     6301 tagcttggta ctaatacgac tcactatagg gagacccaag ctggctaggt aagcttggta 
     6361 c 
//
