LOCUS       pEF6-hsa-miR-138.2        6178 bp    DNA     circular      7-AUG-2009
DEFINITION  LMBP 6905.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|525614396|
COMMENT     VNTNAME|pEF6-hsa-miR-138.2|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

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FEATURES             Location/Qualifiers
     rep_origin      2681..3300
                     /vntifkey="33"
                     /label=pBR322\ori
     promoter        1138..1459
                     /vntifkey="29"
                     /label=SV40\prom
     rep_origin      700..1006
                     /vntifkey="33"
                     /label=f1\ori
     misc_feature    322..384
                     /vntifkey="21"
                     /label=Myc-His
     promoter        6121..6139
                     /vntifkey="30"
                     /label=T7
     promoter        4921..6104
                     /vntifkey="29"
                     /label=EF1a
     CDS             complement(3458..4315)
                     /vntifkey="4"
                     /label=Amp
     CDS             1568..1963
                     /vntifkey="4"
                     /label=bla
BASE COUNT     1385 a      1571 c      1659 g      1563 t 
ORIGIN
        1 gatccgtgtt gtatgggttc atcactgtct caaaaccgga ggagccacag cccagaggag 
       61 gaagccggcg gagttctggt atcgttgctg cagctggtgt tgtgaatcag gccgacgagc 
      121 agcgcatcct cttacccggc tatttcacga caccagggtt gcatcatacc catcctctcc 
      181 aggcgagcct cgtgggaccg ggccggacgc aaggacctgg ggatggggtt ggggtgggag 
      241 tctgtgtgtg aaagcttgtc ctcgaattct gcagatatcc agcacagtgg cggccgctcg 
      301 agtctagagg gcccgcggtt cgaacaaaaa ctcatctcag aagaggatct gaatatgcat 
      361 accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct 
      421 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt 
      481 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg 
      541 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac 
      601 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc 
      661 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 
      721 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 
      781 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 
      841 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 
      901 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 
      961 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 
     1021 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 
     1081 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt 
     1141 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 
     1201 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 
     1261 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 
     1321 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 
     1381 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 
     1441 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg 
     1501 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa 
     1561 ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc 
     1621 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag 
     1681 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga 
     1741 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc 
     1801 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct 
     1861 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt 
     1921 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac ttcgtggccg 
     1981 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt 
     2041 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 
     2101 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa 
     2161 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 
     2221 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct 
     2281 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 
     2341 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 
     2401 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 
     2461 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 
     2521 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
     2581 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 
     2641 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
     2701 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
     2761 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     2821 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     2881 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     2941 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     3001 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     3061 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     3121 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     3181 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     3241 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     3301 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     3361 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     3421 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     3481 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     3541 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 
     3601 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     3661 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     3721 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 
     3781 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     3841 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
     3901 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     3961 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     4021 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 
     4081 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     4141 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     4201 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     4261 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     4321 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     4381 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     4441 cacctgacgt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 
     4501 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 
     4561 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtcgac tctcagtaca 
     4621 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc 
     4681 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc 
     4741 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat 
     4801 acgcgttgac attgattatt gactaggctt ttgcaaaaag ctttgcaaag atggataaag 
     4861 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtgcct 
     4921 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt 
     4981 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg 
     5041 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa 
     5101 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa 
     5161 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt 
     5221 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg 
     5281 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg 
     5341 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg 
     5401 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt 
     5461 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg 
     5521 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc 
     5581 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg 
     5641 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg 
     5701 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat 
     5761 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct 
     5821 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc 
     5881 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg 
     5941 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga 
     6001 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc 
     6061 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaggaatt agcttggtac 
     6121 taatacgact cactataggg agacccaagc tggctaggta agcttggtac cgagctcg 
//
