LOCUS       pEF6-hsa-miR-181c+d        6423 bp    DNA     circular      3-AUG-2009
DEFINITION  LMBP 6907.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|525614475|
COMMENT     VNTNAME|pEF6-hsa-miR-181c+d|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-hsa-miR-181c+d" 0 6423 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 1931477113 0 "" 0 "" "" "" "" "" "" 
COMMENT             "hsa-miR-181c+d" "" 0 0 1 509 1 509 3 511 0 "" "" "" "" "" "" 
COMMENT             (CObList) (CTerm 1 0 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "EcoRI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 1931477113 0 "" 0 "" "" "" "" "" "" "pEF6MycHisC" 
COMMENT             "" 0 0 1 5906 510 6415 1751 1727 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "EcoRI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 1 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AatII" "Acc65I" "AccI" "AflII" "AflIII" "AgeI" "AluI" 
COMMENT         "AlwNI" "ApaI" "ApaLI" "AspI" "AvaI" "AvrII" "BamHI" "BanI" "BbsI" 
COMMENT         "BclI" "BcnI" "BglI" "BglII" "BslI" "Bsp1286I" "BspEI" "BspHI" "BsrBI" 
COMMENT         "BsrGI" "Bst1107I" "Bst98I" "BstEII" "BstNI" "BstXI" "BstYI" "Bsu36I" 
COMMENT         "ClaI" "Csp45I" "DdeI" "DpnI" "DraI" "DraIII" "DrdI" "EaeI" "EagI" 
COMMENT         "Eam1105I" "EarI" "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "Esp3I" "FokI" 
COMMENT         "FspI" "HaeII" "HincII" "HindIII" "HpaI" "HphI" "KasI" "KpnI" "Ksp632I" 
COMMENT         "KspI" "MaeIII" "MamI" "MboI" "MfeI" "MluI" "MnlI" "MscI" "MseI" "MslI" 
COMMENT         "NaeI" "NcoI" "NdeI" "NheI" "NotI" "NruI" "NsiI" "Nsp(7524)I" "NspI" 
COMMENT         "PacI" "PflMI" "PmeI" "PpiI" "PstI" "PvuI" "PvuII" "RsaI" "SacI" 
COMMENT         "SacII" "SalI" "SapI" "Sau3AI" "ScaI" "SfiI" "SinI" "SmaI" "SnaBI" 
COMMENT         "SpeI" "SphI" "SspI" "SstI" "SstII" "StuI" "StyI" "TaqI" "Tth111I" 
COMMENT         "XbaI" "XhoI" "XmaI" "XmnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6423 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #2=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 4696 0 4818 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 6412 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AccI" "gtmkac" 3 0 4 2501 0 2508 0 4818 0 4852 
COMMENT            0 0 "") 
COMMENT        #5=(CRSite (CStringList) "AflII" "cttaag" 2 0 2 388 0 5551 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "AflIII" "acrygt" 2 0 5 1744 0 2240 0 2881 0 
COMMENT            5047 0 5805 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AgeI" "accggt" 2 0 2 607 0 5250 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "AluI" "agct" 3 0 35 148 0 486 0 905 0 1072 0 
COMMENT            1329 0 1649 0 1703 0 1713 0 1916 0 2021 0 2385 0 2518 0 2524 0 2546 
COMMENT            0 2641 0 2705 0 2823 0 3049 0 3185 0 3442 0 3963 0 4063 0 4126 0 
COMMENT            4954 0 5086 0 5132 0 5510 0 5868 0 5998 0 6142 0 6234 0 6358 0 6395 
COMMENT            0 6408 0 6420 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 2 3297 0 4899 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 559 0 5789 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 2 3195 0 4441 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AspI" "gacnnngtc" 5 0 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 543 0 1706 0 5217 0 5319 
COMMENT             0 5505 0 6020 0 6137 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AvrII" "cctagg" 2 0 2 278 0 1685 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 13 724 0 1118 0 2088 0 
COMMENT             2625 0 3722 0 5178 0 5253 0 5626 0 5885 0 5945 0 6110 0 6121 0 6412 
COMMENT             0 0 "") 
COMMENT        #17=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 848 0 1899 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 646 0 1736 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "BcnI" "cccgg" 3 4 11 92 0 351 1 1707 0 1708 1 
COMMENT             2307 1 3261 0 3957 1 4308 0 5939 0 5980 0 6108 1 0 "") 
COMMENT        #20=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 4 3894 0 5943 0 6119 
COMMENT             0 6236 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "BglII" "agatct" 2 0 3 226 0 4831 0 5738 0 0 
COMMENT             "") 
COMMENT        #22=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 32 11 0 85 0 91 0 172 
COMMENT             0 206 0 230 0 279 0 352 0 940 0 1266 0 1593 0 2085 0 2336 0 2729 0 
COMMENT             2903 0 2921 0 3087 0 3366 0 5188 0 5189 0 5263 0 5325 0 5326 0 5441 
COMMENT             0 5512 0 5593 0 5847 0 5920 0 5921 0 5979 0 5991 0 6052 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 16 249 0 267 0 559 0 
COMMENT             877 0 913 0 1088 0 2003 0 2078 0 3199 0 4360 0 4445 0 5183 0 5561 0 
COMMENT             5789 0 6000 0 6422 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 3601 0 4609 0 4731 0 0 
COMMENT             "") 
COMMENT        #26=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 8 542 0 1015 0 2573 0 
COMMENT             2814 1 4615 1 4737 1 5961 1 6029 1 0 "") 
COMMENT        #27=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "Bst1107I" "gtatac" 4 0 1 2502 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "Bst98I" "cttaag" 2 0 2 388 0 5551 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 20 86 0 187 0 240 0 261 0 
COMMENT             718 0 1399 0 1454 0 1471 0 2118 0 2621 0 2909 0 3030 0 3043 0 5431 
COMMENT             0 5480 0 5588 0 5593 0 5890 0 5921 0 6119 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 2 111 0 532 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 14 1 0 226 0 591 0 1731 0 
COMMENT             2339 0 3522 0 3533 0 3619 0 3631 0 4399 0 4416 0 4831 0 5738 0 6282 
COMMENT             0 0 "") 
COMMENT        #34=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 1 180 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "Csp45I" "ttcgaa" 3 0 1 566 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "DdeI" "ctnag" 2 0 19 149 0 180 0 335 0 436 0 
COMMENT             582 0 778 0 887 0 1645 0 2209 0 3156 0 3565 0 3731 0 4271 0 4858 0 
COMMENT             4928 0 4999 0 6074 0 6205 0 6304 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "DpnI" "gatc" 3 0 30 3 0 228 0 593 0 648 0 
COMMENT             1733 0 1738 0 2048 0 2109 0 2123 0 2325 0 2341 0 3449 0 3524 0 3535 
COMMENT             0 3543 0 3621 0 3633 0 3738 0 4079 0 4097 0 4143 0 4401 0 4418 0 
COMMENT             4454 0 4825 0 4833 0 4841 0 5503 0 5740 0 6284 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "DraI" "tttaaa" 4 0 6 637 0 3640 0 3659 0 4351 
COMMENT             0 5110 0 5673 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 3 1162 0 2219 0 6208 
COMMENT             0 0 "") 
COMMENT        #41=(CRSite (CStringList) "DrdI" "gacnnnnnngtc" 8 0 3 1206 0 1901 0 2989 
COMMENT             0 0 "") 
COMMENT        #42=(CRSite (CStringList) "EaeI" "yggccr" 2 0 9 537 0 1815 0 1930 0 2221 
COMMENT             0 2720 0 4162 0 5835 0 5870 0 5970 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "EagI" "cggccg" 2 0 2 537 0 1930 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "Eam1105I" "gacnnnnngtc" 7 0 2 86 0 3774 0 0 
COMMENT             "") 
COMMENT        #45=(CRSite (CStringList) "EarI" "ctcttc" 8 11 4 46 0 581 1 2765 0 4569 
COMMENT             0 0 "") 
COMMENT        #46=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 2 5998 0 6420 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 8 88 0 236 0 414 0 
COMMENT             555 0 1973 0 5785 0 5822 0 6061 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 510 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 522 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "Esp3I" "cgtctcn" 8 12 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "FokI" "ggatg" 15 19 11 183 1 368 0 516 0 877 
COMMENT             0 1536 1 1865 1 2100 1 2333 0 3740 1 3921 1 4208 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 3996 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 7 1004 0 1012 0 2759 0 
COMMENT             3129 0 5600 0 6020 0 6114 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "HincII" "gtyrac" 4 0 5 1750 0 2509 0 4819 0 
COMMENT             4853 0 5053 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 5084 0 6406 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "HpaI" "gttaac" 4 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "HphI" "ggtga" 14 13 13 146 0 221 0 610 1 616 
COMMENT             1 1159 0 1810 0 3617 1 3844 1 4260 0 4466 1 4501 0 6034 1 6048 0 0 
COMMENT             "") #58=(CRSite (CStringList) "KasI" "ggcgcc" 2 0 1 6110 0 0 "") 
COMMENT        #59=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 6416 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "Ksp632I" "ctcttcn" 8 11 4 46 0 581 1 2765 0 
COMMENT             4569 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "KspI" "ccgcgg" 5 0 3 562 0 5426 0 5773 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "MaeIII" "gtnac" 1 0 17 209 0 294 0 362 0 431 
COMMENT             0 969 0 981 0 2394 0 3237 0 3300 0 3416 0 3699 0 4030 0 4088 0 4241 
COMMENT             0 4429 0 6040 0 6090 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "MamI" "gatnnnnatc" 6 0 1 2112 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "MboI" "gatc" 1 0 30 1 0 226 0 591 0 646 0 
COMMENT             1731 0 1736 0 2046 0 2107 0 2121 0 2323 0 2339 0 3447 0 3522 0 3533 
COMMENT             0 3541 0 3619 0 3631 0 3736 0 4077 0 4095 0 4141 0 4399 0 4416 0 
COMMENT             4452 0 4823 0 4831 0 4839 0 5501 0 5738 0 6282 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "MfeI" "caattg" 2 0 2 4980 0 5243 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 5047 0 0 "") 
COMMENT        #67=(CRSite (CStringList) "MnlI" "cctc" 12 11 60 40 0 43 0 49 0 175 1 
COMMENT             210 0 283 0 285 1 323 1 330 1 472 1 479 0 546 1 582 1 663 0 705 0 
COMMENT             750 1 825 1 882 1 1132 0 1616 1 1622 1 1646 0 1652 0 1659 1 1662 1 
COMMENT             1674 1 1794 1 1856 0 2197 1 2197 0 2219 1 2337 0 2522 0 2730 1 2780 
COMMENT             0 2989 1 3063 0 3313 1 3713 1 3794 1 3941 0 4147 0 4914 1 5111 1 
COMMENT             5163 1 5174 0 5226 1 5315 1 5445 0 5533 1 5576 1 5578 0 5809 1 5904 
COMMENT             0 6001 1 6083 0 6135 0 6167 1 6313 0 6342 1 0 "") 
COMMENT        #68=(CRSite (CStringList) "MscI" "tggcca" 4 0 1 1817 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "MseI" "ttaa" 2 0 24 389 0 636 0 949 0 1220 0 
COMMENT             1318 0 1335 0 1346 0 1356 0 1756 0 2652 0 2711 0 3587 0 3639 0 3644 
COMMENT             0 3658 0 3711 0 3946 0 3985 0 4350 0 4888 0 4950 0 5109 0 5552 0 
COMMENT             5672 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "MslI" "caynnnnrtg" 6 0 3 4026 0 4185 0 4544 0 
COMMENT             0 "") 
COMMENT        #71=(CRSite (CStringList) "NaeI" "gccggc" 4 0 3 1056 0 2315 0 5874 0 0 
COMMENT             "") 
COMMENT        #72=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 1592 0 1812 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 537 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "NruI" "tcgcga" 4 0 2 2045 0 5027 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "NsiI" "atgcat" 6 0 3 605 0 1435 0 1507 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "Nsp(7524)I" "rcatgy" 6 0 5 859 0 1433 0 1505 
COMMENT             0 2885 0 5809 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "NspI" "rcatgy" 6 0 5 859 0 1433 0 1505 0 2885 
COMMENT             0 5809 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "PflMI" "ccannnnntgg" 8 0 0 0 "") 
COMMENT        #82=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 1 637 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "PpiI" "gaacnnnnnctc" 26 21 4 592 0 1198 1 
COMMENT             3596 0 4406 1 0 "") 
COMMENT        #84=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 519 0 5488 0 5993 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 2049 0 4144 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 905 0 2021 0 2705 0 0 
COMMENT             "") 
COMMENT        #87=(CRSite (CStringList) "RsaI" "gtac" 3 0 9 4254 0 4863 0 5033 0 5301 
COMMENT             0 5490 0 6105 0 6152 0 6364 0 6414 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "SacI" "gagctc" 6 0 2 6000 0 6422 0 0 "") 
COMMENT        #89=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 3 562 0 5426 0 5773 0 0 
COMMENT             "") 
COMMENT        #90=(CRSite (CStringList) "SalI" "gtcgac" 2 0 3 2507 0 4817 0 4851 0 0 
COMMENT             "") #91=(CRSite (CStringList) "SapI" "gctcttc" 9 12 1 2765 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Sau3AI" "gatc" 1 0 30 1 0 226 0 591 0 646 0 
COMMENT             1731 0 1736 0 2046 0 2107 0 2121 0 2323 0 2339 0 3447 0 3522 0 3533 
COMMENT             0 3541 0 3619 0 3631 0 3736 0 4077 0 4095 0 4141 0 4399 0 4416 0 
COMMENT             4452 0 4823 0 4831 0 4839 0 5501 0 5738 0 6282 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 4254 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "SinI" "ggwcc" 2 0 7 88 0 175 0 414 0 1973 0 
COMMENT             3912 0 4134 0 5138 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 1708 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "SphI" "gcatgc" 6 0 3 859 0 1433 0 1505 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "SspI" "aatatt" 4 0 2 4578 0 4700 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "SstI" "gagctc" 6 0 2 6000 0 6422 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "SstII" "ccgcgg" 5 0 3 562 0 5426 0 5773 0 0 
COMMENT              "") 
COMMENT        #103=(CRSite (CStringList) "StuI" "aggcct" 4 0 4 185 0 1684 0 5543 0 
COMMENT              5586 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "StyI" "ccwwgg" 2 0 6 104 0 278 0 1592 0 1685 
COMMENT              0 1812 0 6256 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "TaqI" "tcga" 2 0 16 162 0 544 0 566 0 655 0 
COMMENT              1124 0 2106 0 2257 0 2508 0 2981 0 4425 0 4818 0 4852 0 5538 0 
COMMENT              5653 0 6127 0 6138 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "Tth111I" "gacnnngtc" 5 0 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 549 0 0 "") 
COMMENT        #108=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 543 0 6137 0 0 "") 
COMMENT        #109=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 1706 0 0 "") 
COMMENT        #110=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 3 1357 0 4373 0 5471 
COMMENT              0 0 "")) 
COMMENT       (CObList 
COMMENT        #111=(CFSignal (CObList) "pBR322 ori" 33 0 0 2926 3545 1 (CStringList) 
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COMMENT        #112=(CFSignal (CObList) "SV40 prom" 29 0 0 1383 1704 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #113=(CFSignal (CObList) "f1 ori" 33 0 0 945 1251 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #114=(CFSignal (CObList) "Myc-His" 21 0 0 567 629 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #115=(CFSignal (CObList) "T7" 30 0 0 6366 6384 1 (CStringList) 
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COMMENT                             "DNA 'pEF6-hsa-miR-181c+d'" 1) 
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COMMENT                             "Local object" 1) 
COMMENT                       #124=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Created: 08/03/09 12:01" 1) 
COMMENT                       #125=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Last Modified: 08/03/09 12:01" 1) 
COMMENT                       #126=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "length: 6423 bp" 1) 
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FEATURES             Location/Qualifiers
     rep_origin      2926..3545
                     /vntifkey="33"
                     /label=pBR322\ori
     promoter        1383..1704
                     /vntifkey="29"
                     /label=SV40\prom
     rep_origin      945..1251
                     /vntifkey="33"
                     /label=f1\ori
     misc_feature    567..629
                     /vntifkey="21"
                     /label=Myc-His
     promoter        6366..6384
                     /vntifkey="30"
                     /label=T7
     promoter        5166..6349
                     /vntifkey="29"
                     /label=EF1a
     CDS             complement(3703..4560)
                     /vntifkey="4"
                     /label=Amp
     CDS             1813..2208
                     /vntifkey="4"
                     /label=bla
BASE COUNT     1432 a      1662 c      1714 g      1615 t 
ORIGIN
        1 gatccagaaa aggggttcct tactctctcc tcctctccct cttcatgctc tctggttccc 
       61 tgccacctac cccacccccg actccaggtc ccggaaaatt tgccaagggt ttgggggaac 
      121 attcaacctg tcggtgagtt tgggcagctc aggcaaacca tcgaccgttg agtggaccct 
      181 gaggcctgga attgccatcc tcctgccggt gactctgacc ttccagatct aggggggcct 
      241 ggggagcccc caatccagcc tgggcacgtc ccctccccta ggccacagcc gaggtcacaa 
      301 tcaacattca ttgttgtcgg tgggttgtga ggactgaggc cagacccacc gggggatgaa 
      361 tgtcactgtg gctgggccag acacggctta aggggaatgg ggactgggga caggaccccc 
      421 caccgccaca gtcactcagc ctgttttttg ccctgacccc aaccactcct ctttggagag 
      481 gagagctggt gtctggaatc tggatgggga attctgcaga tatccagcac agtggcggcc 
      541 gctcgagtct agagggcccg cggttcgaac aaaaactcat ctcagaagag gatctgaata 
      601 tgcataccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg 
      661 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 
      721 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 
      781 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 
      841 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa 
      901 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg 
      961 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 
     1021 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 
     1081 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 
     1141 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 
     1201 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 
     1261 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 
     1321 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt 
     1381 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 
     1441 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 
     1501 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct 
     1561 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 
     1621 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg 
     1681 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca 
     1741 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg 
     1801 aggaactaaa ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca 
     1861 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc 
     1921 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt 
     1981 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc 
     2041 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg 
     2101 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg 
     2161 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agcacttcgt 
     2221 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga 
     2281 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 
     2341 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa 
     2401 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     2461 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta 
     2521 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 
     2581 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 
     2641 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 
     2701 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 
     2761 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
     2821 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 
     2881 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     2941 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     3001 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     3061 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     3121 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     3181 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     3241 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     3301 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     3361 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 
     3421 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     3481 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     3541 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     3601 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     3661 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     3721 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     3781 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     3841 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     3901 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     3961 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     4021 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     4081 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     4141 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     4201 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     4261 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
     4321 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
     4381 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
     4441 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
     4501 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
     4561 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     4621 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     4681 agtgccacct gacgtcaata ttattgaagc atttatcagg gttattgtct catgagcgga 
     4741 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 
     4801 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tcgactctca 
     4861 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg 
     4921 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca 
     4981 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca 
     5041 gatatacgcg ttgacattga ttattgacta ggcttttgca aaaagctttg caaagatgga 
     5101 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag 
     5161 tgcctcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga 
     5221 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa 
     5281 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta 
     5341 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca 
     5401 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt 
     5461 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg 
     5521 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt 
     5581 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg 
     5641 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct 
     5701 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt 
     5761 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg 
     5821 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc 
     5881 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg 
     5941 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc 
     6001 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag 
     6061 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag 
     6121 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt 
     6181 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca 
     6241 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa 
     6301 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt 
     6361 ggtactaata cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc 
     6421 tcg 
//
