LOCUS       pEF6-hsa-miR17+18a+19a+20a+19b.1+92a.1        7004 bp    DNA     circular     12-AUG-2009
DEFINITION  LMBP 6910.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|525614567|
COMMENT     VNTNAME|pEF6-hsa-miR17+18a+19a+20a+19b.1+92a.1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-hsa-miR17+18a+19a+20a+19b.1+92a.1" 0 7004 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "pEF6MycHisC" "" 0 0 1 
COMMENT             5906 1 5906 1751 1727 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "EcoRI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 24981836 0 "" 0 "" "" "" "" "" "" 
COMMENT             "hsa-miR-17+18a+19a+20a+19b.1+92a.1" "" 0 0 1 1098 5907 7004 3 1100 
COMMENT             0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "EcoRI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 1 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AatII" "Acc65I" "AccI" "AflII" "AflIII" "AgeI" "AluI" 
COMMENT         "AlwNI" "ApaI" "ApaLI" "AspI" "AvaI" "AvrII" "BamHI" "BanI" "BbsI" 
COMMENT         "BclI" "BcnI" "BglI" "BglII" "BslI" "Bsp1286I" "BspEI" "BspHI" "BsrBI" 
COMMENT         "BsrGI" "Bst1107I" "Bst98I" "BstEII" "BstNI" "BstXI" "BstYI" "Bsu36I" 
COMMENT         "ClaI" "Csp45I" "DdeI" "DpnI" "DraI" "DraIII" "DrdI" "EaeI" "EagI" 
COMMENT         "Eam1105I" "EarI" "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "Esp3I" "FokI" 
COMMENT         "FspI" "HaeII" "HincII" "HindIII" "HpaI" "HphI" "KasI" "KpnI" "Ksp632I" 
COMMENT         "KspI" "MaeIII" "MamI" "MboI" "MfeI" "MluI" "MnlI" "MscI" "MseI" "MslI" 
COMMENT         "NaeI" "NcoI" "NdeI" "NheI" "NotI" "NruI" "NsiI" "Nsp(7524)I" "NspI" 
COMMENT         "PacI" "PflMI" "PmeI" "PpiI" "PstI" "PvuI" "PvuII" "RsaI" "SacI" 
COMMENT         "SacII" "SalI" "SapI" "Sau3AI" "ScaI" "SfiI" "SinI" "SmaI" "SnaBI" 
COMMENT         "SpeI" "SphI" "SspI" "SstI" "SstII" "StuI" "StyI" "TaqI" "Tth111I" 
COMMENT         "XbaI" "XhoI" "XmaI" "XmnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 7004 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #2=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 4179 0 4301 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 5895 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AccI" "gtmkac" 3 0 4 1984 0 1991 0 4301 0 4335 
COMMENT            0 0 "") #5=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 5034 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "AflIII" "acrygt" 2 0 5 1227 0 1723 0 2364 0 
COMMENT            4530 0 5288 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AgeI" "accggt" 2 0 2 90 0 4733 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "AluI" "agct" 3 0 38 388 0 555 0 812 0 1132 0 
COMMENT            1186 0 1196 0 1399 0 1504 0 1868 0 2001 0 2007 0 2029 0 2124 0 2188 
COMMENT            0 2306 0 2532 0 2668 0 2925 0 3446 0 3546 0 3609 0 4437 0 4569 0 
COMMENT            4615 0 4993 0 5351 0 5481 0 5625 0 5717 0 5841 0 5878 0 5891 0 5903 
COMMENT            0 6119 0 6488 0 6567 0 6581 0 6657 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 4 2780 0 4382 0 6158 0 
COMMENT            6491 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 5272 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 2 2678 0 3924 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AspI" "gacnnngtc" 5 0 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 8 34 0 1189 0 4700 0 4802 
COMMENT             0 4988 0 5503 0 5620 0 6124 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 1168 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 5907 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 13 207 0 601 0 1571 0 2108 
COMMENT             0 3205 0 4661 0 4736 0 5109 0 5368 0 5428 0 5593 0 5604 0 5895 0 0 
COMMENT             "") 
COMMENT        #17=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 331 0 1382 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 129 0 1219 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "BcnI" "cccgg" 3 4 10 1190 0 1191 1 1790 1 
COMMENT             2744 0 3440 1 3791 0 5422 0 5463 0 5591 1 6803 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 4 3377 0 5426 0 5602 
COMMENT             0 5719 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "BglII" "agatct" 2 0 2 4314 0 5221 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 24 423 0 749 0 1076 0 
COMMENT             1568 0 1819 0 2212 0 2386 0 2404 0 2570 0 2849 0 4671 0 4672 0 4746 
COMMENT             0 4808 0 4809 0 4924 0 4995 0 5076 0 5330 0 5403 0 5404 0 5462 0 
COMMENT             5474 0 5535 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 15 360 0 396 0 571 0 
COMMENT             1486 0 1561 0 2682 0 3843 0 3928 0 4666 0 5044 0 5272 0 5483 0 5905 
COMMENT             0 6240 0 6551 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 3084 0 4092 0 4214 0 0 
COMMENT             "") 
COMMENT        #26=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 8 33 0 498 0 2056 0 2297 
COMMENT             1 4098 1 4220 1 5444 1 5512 1 0 "") 
COMMENT        #27=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 6887 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "Bst1107I" "gtatac" 4 0 1 1985 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "Bst98I" "cttaag" 2 0 1 5034 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 1 39 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 17 201 0 882 0 937 0 954 0 
COMMENT             1601 0 2104 0 2392 0 2513 0 2526 0 4914 0 4963 0 5071 0 5076 0 5373 
COMMENT             0 5404 0 5602 0 6468 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 23 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 13 74 0 1214 0 1822 0 
COMMENT             3005 0 3016 0 3102 0 3114 0 3882 0 3899 0 4314 0 5221 0 5765 0 5907 
COMMENT             0 0 "") #34=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "Csp45I" "ttcgaa" 3 0 1 49 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "DdeI" "ctnag" 2 0 17 65 0 261 0 370 0 1128 0 
COMMENT             1692 0 2639 0 3048 0 3214 0 3754 0 4341 0 4411 0 4482 0 5557 0 5688 
COMMENT             0 5787 0 6184 0 6232 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "DpnI" "gatc" 3 0 30 76 0 131 0 1216 0 1221 0 
COMMENT             1531 0 1592 0 1606 0 1808 0 1824 0 2932 0 3007 0 3018 0 3026 0 3104 
COMMENT             0 3116 0 3221 0 3562 0 3580 0 3626 0 3884 0 3901 0 3937 0 4308 0 
COMMENT             4316 0 4324 0 4986 0 5223 0 5767 0 5909 0 6761 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "DraI" "tttaaa" 4 0 7 120 0 3123 0 3142 0 3834 
COMMENT             0 4593 0 5156 0 6146 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 3 645 0 1702 0 5691 0 
COMMENT             0 "") 
COMMENT        #41=(CRSite (CStringList) "DrdI" "gacnnnnnngtc" 8 0 3 689 0 1384 0 2472 
COMMENT             0 0 "") 
COMMENT        #42=(CRSite (CStringList) "EaeI" "yggccr" 2 0 9 28 0 1298 0 1413 0 1704 
COMMENT             0 2203 0 3645 0 5318 0 5353 0 5453 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "EagI" "cggccg" 2 0 2 28 0 1413 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "Eam1105I" "gacnnnnngtc" 7 0 1 3257 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "EarI" "ctcttc" 8 11 3 64 1 2248 0 4052 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 2 5481 0 5903 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 5 1456 0 5268 0 5305 
COMMENT             0 5544 0 6155 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 13 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "Esp3I" "cgtctcn" 8 12 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "FokI" "ggatg" 15 19 10 360 0 1019 1 1348 1 
COMMENT             1583 1 1816 0 3223 1 3404 1 3691 1 6253 1 6637 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 3479 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 7 487 0 495 0 2242 0 2612 
COMMENT             0 5083 0 5503 0 5597 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "HincII" "gtyrac" 4 0 5 1233 0 1992 0 4302 0 
COMMENT             4336 0 4536 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 4567 0 5889 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "HpaI" "gttaac" 4 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "HphI" "ggtga" 14 13 14 33 1 93 1 99 1 642 0 
COMMENT             1293 0 3100 1 3327 1 3743 0 3949 1 3984 0 5517 1 5531 0 5997 1 6124 
COMMENT             0 0 "") 
COMMENT        #58=(CRSite (CStringList) "KasI" "ggcgcc" 2 0 1 5593 0 0 "") 
COMMENT        #59=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 5899 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "Ksp632I" "ctcttcn" 8 11 3 64 1 2248 0 4052 0 
COMMENT             0 "") 
COMMENT        #61=(CRSite (CStringList) "KspI" "ccgcgg" 5 0 2 4909 0 5256 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "MaeIII" "gtnac" 1 0 20 39 0 452 0 464 0 1877 
COMMENT             0 2720 0 2783 0 2899 0 3182 0 3513 0 3571 0 3724 0 3912 0 5523 0 
COMMENT             5573 0 6026 0 6112 0 6481 0 6611 0 6828 0 6919 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "MamI" "gatnnnnatc" 6 0 2 1595 0 6219 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "MboI" "gatc" 1 0 30 74 0 129 0 1214 0 1219 0 
COMMENT             1529 0 1590 0 1604 0 1806 0 1822 0 2930 0 3005 0 3016 0 3024 0 3102 
COMMENT             0 3114 0 3219 0 3560 0 3578 0 3624 0 3882 0 3899 0 3935 0 4306 0 
COMMENT             4314 0 4322 0 4984 0 5221 0 5765 0 5907 0 6759 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "MfeI" "caattg" 2 0 2 4463 0 4726 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 4530 0 0 "") 
COMMENT        #67=(CRSite (CStringList) "MnlI" "cctc" 12 11 52 30 1 65 1 146 0 188 0 
COMMENT             233 1 308 1 365 1 615 0 1099 1 1105 1 1129 0 1135 0 1142 1 1145 1 
COMMENT             1157 1 1277 1 1339 0 1680 1 1680 0 1702 1 1820 0 2005 0 2213 1 2263 
COMMENT             0 2472 1 2546 0 2796 1 3196 1 3277 1 3424 0 3630 0 4397 1 4594 1 
COMMENT             4646 1 4657 0 4709 1 4798 1 4928 0 5016 1 5059 1 5061 0 5292 1 5387 
COMMENT             0 5484 1 5566 0 5618 0 5650 1 5796 0 5825 1 5946 1 6133 0 6335 0 0 
COMMENT             "") #68=(CRSite (CStringList) "MscI" "tggcca" 4 0 1 1300 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "MseI" "ttaa" 2 0 30 119 0 432 0 703 0 801 0 
COMMENT             818 0 829 0 839 0 1239 0 2135 0 2194 0 3070 0 3122 0 3127 0 3141 0 
COMMENT             3194 0 3429 0 3468 0 3833 0 4371 0 4433 0 4592 0 5035 0 5155 0 5959 
COMMENT             0 6145 0 6304 0 6553 0 6850 0 6882 0 6907 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "MslI" "caynnnnrtg" 6 0 5 3509 0 3668 0 4027 0 
COMMENT             6110 0 6502 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "NaeI" "gccggc" 4 0 3 539 0 1798 0 5357 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 1075 0 1295 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 6563 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 28 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "NruI" "tcgcga" 4 0 2 1528 0 4510 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "NsiI" "atgcat" 6 0 3 88 0 918 0 990 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "Nsp(7524)I" "rcatgy" 6 0 5 342 0 916 0 988 0 
COMMENT             2368 0 5292 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "NspI" "rcatgy" 6 0 5 342 0 916 0 988 0 2368 0 
COMMENT             5292 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "PflMI" "ccannnnntgg" 8 0 0 0 "") 
COMMENT        #82=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 1 120 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "PpiI" "gaacnnnnnctc" 26 21 4 75 0 681 1 3079 
COMMENT             0 3889 1 0 "") 
COMMENT        #84=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 10 0 4971 0 5476 0 6089 
COMMENT             0 0 "") 
COMMENT        #85=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 1532 0 3627 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "PvuII" "cagctg" 4 0 5 388 0 1504 0 2188 0 
COMMENT             6119 0 6657 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "RsaI" "gtac" 3 0 12 3737 0 4346 0 4516 0 4784 
COMMENT             0 4973 0 5588 0 5635 0 5847 0 5897 0 6439 0 6559 0 6889 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "SacI" "gagctc" 6 0 2 5483 0 5905 0 0 "") 
COMMENT        #89=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 4909 0 5256 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "SalI" "gtcgac" 2 0 3 1990 0 4300 0 4334 0 0 
COMMENT             "") #91=(CRSite (CStringList) "SapI" "gctcttc" 9 12 1 2248 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Sau3AI" "gatc" 1 0 30 74 0 129 0 1214 0 1219 
COMMENT             0 1529 0 1590 0 1604 0 1806 0 1822 0 2930 0 3005 0 3016 0 3024 0 
COMMENT             3102 0 3114 0 3219 0 3560 0 3578 0 3624 0 3882 0 3899 0 3935 0 4306 
COMMENT             0 4314 0 4322 0 4984 0 5221 0 5765 0 5907 0 6759 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "ScaI" "agtact" 4 0 2 3737 0 6559 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "SinI" "ggwcc" 2 0 4 1456 0 3395 0 3617 0 4621 
COMMENT             0 0 "") 
COMMENT        #96=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 1191 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "SphI" "gcatgc" 6 0 3 342 0 916 0 988 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "SspI" "aatatt" 4 0 4 4061 0 4183 0 6855 0 
COMMENT              6905 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "SstI" "gagctc" 6 0 2 5483 0 5905 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "SstII" "ccgcgg" 5 0 2 4909 0 5256 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "StuI" "aggcct" 4 0 3 1167 0 5026 0 5069 0 0 
COMMENT              "") 
COMMENT        #104=(CRSite (CStringList) "StyI" "ccwwgg" 2 0 4 1075 0 1168 0 1295 0 
COMMENT              5739 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "TaqI" "tcga" 2 0 17 35 0 49 0 138 0 607 0 
COMMENT              1589 0 1740 0 1991 0 2464 0 3908 0 4301 0 4335 0 5021 0 5136 0 
COMMENT              5610 0 5621 0 6452 0 6869 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "Tth111I" "gacnnngtc" 5 0 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") 
COMMENT        #108=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 34 0 5620 0 0 "") 
COMMENT        #109=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 1189 0 0 "") 
COMMENT        #110=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 3 840 0 3856 0 4954 0 
COMMENT              0 "")) 
COMMENT       (CObList 
COMMENT        #111=(CFSignal (CObList) "pBR322 ori" 33 0 0 2409 3028 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #112=(CFSignal (CObList) "SV40 prom" 29 0 0 866 1187 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #113=(CFSignal (CObList) "f1 ori" 33 0 0 428 734 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #114=(CFSignal (CObList) "Myc-His" 21 0 0 50 112 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #115=(CFSignal (CObList) "T7" 30 0 0 5849 5867 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #116=(CFSignal (CObList) "EF1a" 29 0 0 4649 5832 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #117=(CFSignal (CObList) "Amp" 4 0 1 3186 4043 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #118=(CFSignal (CObList) "bla" 4 0 0 1296 1691 1 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #119=(CFSignal (CObList) "miR-17" 21 0 0 6046 6068 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #120=(CFSignal (CObList) "miR-18a" 21 0 0 6184 6206 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #121=(CFSignal (CObList) "miR-19a" 21 0 0 6367 6389 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #122=(CFSignal (CObList) "miR-20a" 21 0 0 6500 6522 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #123=(CFSignal (CObList) "miR-19b.1" 21 0 0 6673 6695 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #124=(CFSignal (CObList) "miR-92a.1" 21 0 0 6789 6810 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #125=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT              (CObjectList 
COMMENT               #126=(CRefLinePar 
COMMENT                     (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                      "pEF6-hsa-miR17+18a+19a+20a+19b.1+92a.1" 2) 5 "" 0 4) 
COMMENT               #127=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #128=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "DNA 'pEF6-hsa-miR17+18a+19a+20a+19b.1+92a.1'" 1) 
COMMENT                       #129=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Local object" 1) 
COMMENT                       #130=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Created: 08/03/09 12:02" 1) 
COMMENT                       #131=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Last Modified: 08/12/09 02:48" 1) 
COMMENT                       #132=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "length: 7004 bp" 1) 
COMMENT                       #133=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "storage type: Basic" 1) 
COMMENT                       #134=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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FEATURES             Location/Qualifiers
     rep_origin      2409..3028
                     /vntifkey="33"
                     /label=pBR322\ori
     promoter        866..1187
                     /vntifkey="29"
                     /label=SV40\prom
     rep_origin      428..734
                     /vntifkey="33"
                     /label=f1\ori
     misc_feature    50..112
                     /vntifkey="21"
                     /label=Myc-His
     promoter        5849..5867
                     /vntifkey="30"
                     /label=T7
     promoter        4649..5832
                     /vntifkey="29"
                     /label=EF1a
     CDS             complement(3186..4043)
                     /vntifkey="4"
                     /label=Amp
     CDS             1296..1691
                     /vntifkey="4"
                     /label=bla
     misc_feature    6046..6068
                     /vntifkey="21"
                     /label=miR-17
     misc_feature    6184..6206
                     /vntifkey="21"
                     /label=miR-18a
     misc_feature    6367..6389
                     /vntifkey="21"
                     /label=miR-19a
     misc_feature    6500..6522
                     /vntifkey="21"
                     /label=miR-20a
     misc_feature    6673..6695
                     /vntifkey="21"
                     /label=miR-19b.1
     misc_feature    6789..6810
                     /vntifkey="21"
                     /label=miR-92a.1
BASE COUNT     1632 a      1678 c      1812 g      1882 t 
ORIGIN
        1 aattctgcag atatccagca cagtggcggc cgctcgaggt cacccattcg aacaaaaact 
       61 catctcagaa gaggatctga atatgcatac cggtcatcat caccatcacc attgagttta 
      121 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc 
      181 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga 
      241 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca 
      301 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc 
      361 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg 
      421 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 
      481 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 
      541 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 
      601 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 
      661 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 
      721 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 
      781 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta 
      841 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga 
      901 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 
      961 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 
     1021 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 
     1081 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag 
     1141 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt 
     1201 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg 
     1261 gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag 
     1321 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact 
     1381 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat 
     1441 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg 
     1501 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc 
     1561 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga 
     1621 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg 
     1681 tgtgggaggg ctaagcactt cgtggccgag gagcaggact gacacgtgct acgagatttc 
     1741 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 
     1801 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt 
     1861 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 
     1921 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 
     1981 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 
     2041 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 
     2101 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 
     2161 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 
     2221 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
     2281 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
     2341 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     2401 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     2461 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     2521 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     2581 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc 
     2641 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     2701 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     2761 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     2821 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
     2881 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     2941 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     3001 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     3061 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     3121 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     3181 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     3241 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     3301 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     3361 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     3421 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     3481 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     3541 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     3601 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     3661 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     3721 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     3781 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
     3841 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     3901 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     3961 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     4021 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     4081 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     4141 gttccgcgca catttccccg aaaagtgcca cctgacgtca atattattga agcatttatc 
     4201 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     4261 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc 
     4321 cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta 
     4381 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac 
     4441 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc 
     4501 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctaggctttt 
     4561 gcaaaaagct ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg 
     4621 gaccttctag gtcttgaaag gagtgcctcg tgaggctccg gtgcccgtca gtgggcagag 
     4681 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc 
     4741 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 
     4801 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 
     4861 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc 
     4921 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt 
     4981 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     5041 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     5101 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     5161 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     5221 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     5281 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     5341 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     5401 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     5461 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     5521 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     5581 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     5641 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     5701 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     5761 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     5821 caggtgtcgt gaggaattag cttggtacta atacgactca ctatagggag acccaagctg 
     5881 gctaggtaag cttggtaccg agctcggatc cgattatgct gaatttgtat ggtttatagt 
     5941 tgttagagtt tgaggtgtta attctaatta tctatttcaa atttagcagg aaaaaagaga 
     6001 acatcacctt gtaaaactga agattgtgac cagtcagaat aatgtcaaag tgcttacagt 
     6061 gcaggtagtg atatgtgcat ctactgcagt gaaggcactt gtagcattat ggtgacagct 
     6121 gcctcgggaa gccaagttgg gctttaaagt gcagggcctg ctgatgttga gtgctttttg 
     6181 ttctaaggtg catctagtgc agatagtgaa gtagattagc atctactgcc ctaagtgctc 
     6241 cttctggcat aagaagttat gtattcatcc aataattcaa gccaagcaag tatataggtg 
     6301 ttttaatagt ttttgtttgc agtcctctgt tagttttgca tagttgcact acaagaagaa 
     6361 tgtagttgtg caaatctatg caaaactgat ggtggcctgc tatttccttc aagtgaatga 
     6421 tttttactaa ttttgtgtac ttttattgtg tcgatgtaga atctgcctgg tctatctgat 
     6481 gtgacagctt ctgtagcact aaagtgctta tagtgcaggt agtgtttagt tatctactgc 
     6541 attatgagca cttaaagtac tgctagctgt agaactccag cttcggcctg tcgcccaatc 
     6601 aaactgtcct gttactgaac actgttctat ggttagtttt gcaggtttgc atccagctgt 
     6661 gtgatattct gctgtgcaaa tccatgcaaa actgactgtg gtagtgaaaa gtctgtagaa 
     6721 aagtaaggga aactcaaacc cctttctaca caggttggga tcggttgcaa tgctgtgttt 
     6781 ctgtatggta ttgcacttgt cccggcctgt tgagtttggt ggggattgtg accagaagat 
     6841 tttgaaaatt aaatattact gaagatttcg acttccactg ttaaatgtac aagatacatg 
     6901 aaatattaaa gaaaatgtgt aactttttgt gtaaatacat cttgtcttgt tttcattcaa 
     6961 aaacatttca cttttggggt tgcgtgtcag atttggcagt atag 
//
