LOCUS       pIRES-hyg-MALT1-C464A        8237 bp    DNA     circular     24-FEB-2011
DEFINITION  LMBP 6924.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6924
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 24-FEB-2011
            
            
            
COMMENT     VNTDATE|582732372|
COMMENT     VNTNAME|pIRES-hyg-MALT1 C464A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BamHI" "BstXI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 3449 3744 1 8237 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 908 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 2427 0 0 "")) 
COMMENT       (CObList 
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COMMENT            (CStringList) (CStringList) 1 1 1 1 "232..818") 
COMMENT        #3=(CFSignal (CObList) "INT" 15 0 0 3449 3744 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "938..1233") 
COMMENT        #4=(CFSignal (CObList) "IRES" 21 0 0 3784 4359 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1273..1848") 
COMMENT        #5=(CFSignal (CObList) "hygromycin resistance" 4 0 0 4383 5417 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1872..2906") 
COMMENT        #6=(CFSignal (CObList) "BGH polyA" 43 0 0 5731 5954 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3220..3443") 
COMMENT        #7=(CFSignal (CObList) "pMB1 ori" 33 0 1 6301 6701 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3790..4190)") 
COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 7241 8101 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(4730..5590)") 
COMMENT        #9=(CFSignal (CObList) "Myc epitope" 22 0 0 3392 3421 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #10=(CFSignal (CObList) "His-tag" 22 0 0 3425 3442 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2033..2050") 
COMMENT        #11=(CFSignal (CObList) "MALT1" 4 0 0 917 3388 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #12=(CFSignal (CObList) "T7 promoter" 30 0 0 863 879 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #13=(CFSignal (CObList) "lac promoter" 30 0 1 6123 6206 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "C464A" 62 0 0 2306 2308 0 (CStringList) 
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COMMENT                          "DNA 'pIRES-hyg-MALT1 C464A'" 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 02/24/11 02:06" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 02/24/11 02:06" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8237 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 19-MAR-1980" 1))) 
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FEATURES             Location/Qualifiers
     promoter        235..818
                     /vntifkey="30"
                     /label=hCMV-IE\promoter\and\enhancer
     intron          3449..3744
                     /vntifkey="15"
                     /label=INT
                     /note="synthetic intron (IVS)"
     misc_feature    3784..4359
                     /vntifkey="21"
                     /label=IRES
                     /note="internal ribosome entry site"
     CDS             4383..5417
                     /vntifkey="4"
                     /label=hygromycin\resistance
                     /note="antibiotic resistance gene; coding sequence"
     terminator      5731..5954
                     /vntifkey="43"
                     /label=BGH\polyA
     rep_origin      complement(6301..6701)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(7241..8101)
                     /vntifkey="4"
                     /label=ampicillin\resistance
                     /note="antibiotic resistance gene; coding sequence"
     misc_marker     3392..3421
                     /vntifkey="22"
                     /label=Myc\epitope
     misc_marker     3425..3442
                     /vntifkey="22"
                     /label=His-tag
     CDS             917..3388
                     /vntifkey="4"
                     /label=MALT1
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(6123..6206)
                     /vntifkey="30"
                     /label=lac\promoter
     mutation        2306..2308
                     /vntifkey="62"
                     /label=C464A
                     /note="WT: TGT"
BASE COUNT     2063 a      1992 c      2103 g      2066 t        13 others
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccnnnnatgt cgctgttggg ggacccgcta caggccctgc cgccctcggc 
      961 cgcccccacg gggccgctgc tcgcccctcc ggccggcgcg accctcaacc gcctgcggga 
     1021 gccgctgctg cggaggctca gcgagctcct ggatcaggcg cccgagggcc ggggctggag 
     1081 gagactggcg gagctggcgg ggagtcgcgg gcgcctccgc ctcagttgcc tagacctgga 
     1141 gcagtgttct cttaaggtac tggagcctga aggaagcccc agcctgtgtc tgctgaagtt 
     1201 aatgggtgaa aaaggttgca cagtcacaga attgagtgat ttcctgcagg ctatggaaca 
     1261 cactgaagtt cttcagcttc tcagcccccc aggaataaag attactgtaa acccagagtc 
     1321 aaaggcagtc ttggctggac agtttgtgaa actgtgttgc cgggcaactg gacatccttt 
     1381 tgttcaatat cagtggttca aaatgaataa agagattcca aatggaaata catcagagct 
     1441 tatttttaat gcagtgcatg taaaagatgc aggcttttat gtctgtcgag ttaataacaa 
     1501 tttcaccttt gaattcagcc agtggtcaca gctggatgtt tgcgacatcc cagagagctt 
     1561 ccagagaagt gttgatggcg tctctgaatc caagttgcaa atctgtgttg aaccaacttc 
     1621 ccaaaagctg atgccaggca gcacattggt tttacagtgt gttgctgttg gaagccctat 
     1681 tcctcactac cagtggttca aaaatgaatt accattaaca catgagacca aaaagctata 
     1741 catggtgcct tatgtggatt tggaacacca aggaacctac tggtgtcatg tatataatga 
     1801 tcgagacagt caagatagca agaaggtaga aatcatcata ggaagaacag atgaggcagt 
     1861 ggagtgcact gaagatgaat taaataatct tggtcatcct gataataaag agcaaacaac 
     1921 tgaccagcct ttggcgaagg acaaggttgc ccttttgata ggaaatatga attaccggga 
     1981 gcaccccaag ctcaaagctc ctttggtgga tgtgtacgaa ttgactaact tactgagaca 
     2041 gctggacttc aaagtggttt cactgttgga tcttactgaa tatgagatgc gtaatgctgt 
     2101 ggatgagttt ttactccttt tagacaaggg agtatatggg ttattatatt atgcaggaca 
     2161 tggttatgaa aattttggga acagcttcat ggtccccgtt gatgctccaa atccatatag 
     2221 gtctgaaaat tgtctgtgtg tacaaaatat actgaaattg atgcaagaaa aagaaactgg 
     2281 acttaatgtg ttcttattgg atatggctag gaaaagaaat gactacgatg ataccattcc 
     2341 aatcttggat gcactaaaag tcaccgccaa tattgtgttt ggatatgcca cgtgtcaagg 
     2401 agcagaagct tttgaaatcc agcattctgg attggcaaat ggaatcttta tgaaattttt 
     2461 aaaagacaga ttattagaag ataagaaaat cactgtgtta ctggatgaag ttgcagaaga 
     2521 tatgggtaag tgtcacctta ccaaaggcaa acaggctcta gagattcgaa gtagtttatc 
     2581 tgagaagaga gcacttactg atccaataca gggaacagaa tattctgctg aatctcttgt 
     2641 gcggaatcta cagtgggcca aggctcatga acttccagaa agtatgtgtc ttaagtttga 
     2701 ctgtggtgtt cagattcaat taggatttgc agctgagttt tccaatgtca tgatcatcta 
     2761 tacaagtata gtttacaaac caccggagat aataatgtgt gatgcctacg ttactgattt 
     2821 tccacttgat ctagatattg atccaaaaga tgcaaataaa ggcacacctg aagaaactgg 
     2881 cagctacttg gtatcaaagg atcttcccaa gcattgcctc tataccagac tcagttcact 
     2941 gcaaaaatta aaggaacatc tagtcttcac agtatgttta tcatatcagt actcaggatt 
     3001 ggaagatact gtagaggaca agcaggaagt gaatgttggg aaacctctca ttgctaaatt 
     3061 agacatgcat cgaggtttgg gaaggaagac ttgctttcaa acttgtctta tgtctaatgg 
     3121 tccttaccag agttctgcag ccacctcagg aggagcaggg cattatcact cattgcaaga 
     3181 cccattccat ggtgtttacc attcacatcc tggtaatcca agtaatgtta caccagcaga 
     3241 tagctgtcat tgcagccgga ctccagatgc atttatttca agtttcgctc accatgcttc 
     3301 atgtcatttt agtagaagta atgtgccagt agagacaact gatgaaatac catttagttt 
     3361 ctctgacagg ctcagaattt ctgaaaaann ngaacaaaaa ctcatctcag aagaggatct 
     3421 gnnncatcat caccatcacc atnnnctgga attaattcgc tgtctgcgag ggccagctgt 
     3481 tggggtgagt actccctctc aaaagcgggc atgacttctg cgctaagatt gtcagtttcc 
     3541 aaaaacgagg aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgcg 
     3601 tccatctggt cagaaaagac aatctttttg ttgtcaagct tgaggtgtgg caggcttgag 
     3661 atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc acaggtgtcc 
     3721 actcccaggt ccaactgcag gtcgatcgag catgcatcta gggcggccgc actagaggaa 
     3781 ttcgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc 
     3841 ggtgtgtgtt tgtctatatg tgattttcca ccatattgcc gtcttttggc aatgtgaggg 
     3901 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca 
     3961 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa 
     4021 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt 
     4081 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt 
     4141 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtagtca 
     4201 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggaatctga tctggggcct 
     4261 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa agctctaggc cccccgaacc 
     4321 acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac cccgtaccaa 
     4381 agatggatag atccggaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg 
     4441 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt 
     4501 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt 
     4561 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag 
     4621 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg 
     4681 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg 
     4741 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg 
     4801 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc 
     4861 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg 
     4921 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg 
     4981 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact 
     5041 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc 
     5101 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg 
     5161 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga 
     5221 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg 
     5281 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct 
     5341 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc 
     5401 cgagggcaaa ggaatagagt agatgccgac cgaacaagag ctgatttcga gaacgcctca 
     5461 gccagcaact cgcgcgagcc tagcaaggca aatgcgagag aacggcctta cgcttggtgg 
     5521 cacagttctc gtccacagtt cgctaagctc gctcggctgg gtcgcgggag ggccggtcgc 
     5581 agtgattcag gcccttctgg attgtgttgg tccccagggc acgattgtca tgcccacgca 
     5641 ctcgggtgat ctgactgatc ccgcagattg gagatcgccg cccgtgcctg ccgattgggt 
     5701 gcagatctag agctcgctga tcagcctcga ctgtgcctct agttgccagc catctgttgt 
     5761 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 
     5821 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 
     5881 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 
     5941 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgagtg cattctagtt 
     6001 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct 
     6061 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 
     6121 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 
     6181 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 
     6241 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 
     6301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
     6361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
     6421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
     6481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
     6541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
     6601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
     6661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
     6721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
     6781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
     6841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
     6901 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 
     6961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
     7021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
     7081 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
     7141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 
     7201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 
     7261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 
     7321 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 
     7381 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 
     7441 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 
     7501 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 
     7561 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 
     7621 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 
     7681 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 
     7741 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 
     7801 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 
     7861 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 
     7921 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 
     7981 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 
     8041 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 
     8101 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 
     8161 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 
     8221 aagtgccacc tgacgtc 
//
