LOCUS       pLKO.1-puro-shRNA      7086 bp    DNA     circular UNA 22-OCT-2021
DEFINITION  LMBP 7210.
COMMENT     Accession number: LMBP 7210
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 22-OCT-2021
FEATURES             Location/Qualifiers
     misc_feature    1..48
                     /label=shRNA\insert
     misc_feature    99..114
                     /label=HIV-1\cPPT
     promoter        261..612
                     /label=hPGK1\promoter
     CDS             793..1392
                     /label=puromycin\resistance
     misc_feature    1503..1518
                     /label=HIV-1\cPPT
     terminator      1519..1753
                     /label=RSV/HIV-1\dU3/3'\LTR
     terminator      1825..1899
                     /label=SV40\polyA\early
     terminator      complement(1825..1899)
                     /label=SV40\polyA\late
     rep_origin      2035..2110
                     /label=SV40\ori
     promoter        complement(2144..2160)
                     /label=T7\promoter
     rep_origin      2328..2783
                     /label=f1\ori
     CDS             2914..3774
                     /label=ampicillin\resistance
     rep_origin      4315..4717
                     /label=pMB1\ori
     promoter        4809..4892
                     /label=lac\promoter
     promoter        4956..4972
                     /label=T3\promoter
     promoter        5081..5409
                     /label=RSV/HIV-1\5'\LTR
     misc_feature    5520..5564
                     /label=HIV-1\psi\packaging\signal
     misc_feature    6075..6308
                     /label=HIV-1\Rev\response\element
     promoter        6836..7082
                     /label=hU6\promoter
ORIGIN
        1 NNNNNNNNNN NNNNNNNNNN NCTCGAGNNN NNNNNNNNNN NNNNNNNNTT TTTGAATTCT
       61 CGACCTCGAG ACAAATGGCA GTATTCATCC ACAATTTTAA AAGAAAAGGG GGGATTGGGG
      121 GGTACAGTGC AGGGGAAAGA ATAGTAGACA TAATAGCAAC AGACATACAA ACTAAAGAAT
      181 TACAAAAACA AATTACAAAA ATTCAAAATT TTCGGGTTTA TTACAGGGAC AGCAGAGATC
      241 CACTTTGGCC GCGGCTCGAG GGGGTTGGGG TTGCGCCTTT TCCAAGGCAG CCCTGGGTTT
      301 GCGCAGGGAC GCGGCTGCTC TGGGCGTGGT TCCGGGAAAC GCAGCGGCGC CGACCCTGGG
      361 TCTCGCACAT TCTTCACGTC CGTTCGCAGC GTCACCCGGA TCTTCGCCGC TACCCTTGTG
      421 GGCCCCCCGG CGACGCTTCC TGCTCCGCCC CTAAGTCGGG AAGGTTCCTT GCGGTTCGCG
      481 GCGTGCCGGA CGTGACAAAC GGAAGCCGCA CGTCTCACTA GTACCCTCGC AGACGGACAG
      541 CGCCAGGGAG CAATGGCAGC GCGCCGACCG CGATGGGCTG TGGCCAATAG CGGCTGCTCA
      601 GCAGGGCGCG CCGAGAGCAG CGGCCGGGAA GGGGCGGTGC GGGAGGCGGG GTGTGGGGCG
      661 GTAGTGTGGG CCCTGTTCCT GCCCGCGCGG TGTTCCGCAT TCTGCAAGCC TCCGGAGCGC
      721 ACGTCGGCAG TCGGCTCCCT CGTTGACCGA ATCACCGACC TCTCTCCCCA GGGGGATCCA
      781 CCGGAGCTTA CCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC
      841 CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC
      901 GTCGATCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC
      961 GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG
     1021 ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC
     1081 GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC
     1141 CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC
     1201 AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG
     1261 CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC
     1321 ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG
     1381 CCCGGTGCCT GACGCCCGCC CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC
     1441 CATGCATCGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT
     1501 TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATCTGCTT
     1561 TTTGCTTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA
     1621 CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT
     1681 GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG
     1741 AAAATCTCTA GCAGTAGTAG TTCATGTCAT CTTATTATTC AGTATTTATA ACTTGCAAAG
     1801 AAATGAATAT CAGAGAGTGA GAGGAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA
     1861 AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT
     1921 TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGCTCT AGCTATCCCG CCCCTAACTC
     1981 CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA
     2041 TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT
     2101 GAGGAGGCTT TTTTGGAGGC CTAGGGACGT ACCCAATTCG CCCTATAGTG AGTCGTATTA
     2161 CGCGCGCTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA
     2221 ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG
     2281 CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGGACG CGCCCTGTAG
     2341 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG
     2401 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT
     2461 TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA
     2521 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA
     2581 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA
     2641 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC
     2701 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA
     2761 CAAAATATTA ACGCTTACAA TTTAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT
     2821 ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA
     2881 TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC
     2941 CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG
     3001 AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC
     3061 AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT
     3121 TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC
     3181 GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG
     3241 CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT
     3301 AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT
     3361 TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA
     3421 GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC
     3481 AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG
     3541 GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT
     3601 GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA
     3661 GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT
     3721 GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA
     3781 GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG
     3841 ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG
     3901 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT
     3961 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG
     4021 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA
     4081 CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA
     4141 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG
     4201 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC
     4261 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA
     4321 TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG
     4381 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC
     4441 GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG
     4501 TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG
     4561 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT
     4621 GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC
     4681 GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC
     4741 CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG
     4801 GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA
     4861 CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA
     4921 GGAAACAGCT ATGACCATGA TTACGCCAAG CGCGCAATTA ACCCTCACTA AAGGGAACAA
     4981 AAGCTGGAGC TGCAAGCTTA ATGTAGTCTT ATGCAATACT CTTGTAGTCT TGCAACATGG
     5041 TAACGATGAG TTAGCAACAT GCCTTACAAG GAGAGAAAAA GCACCGTGCA TGCCGATTGG
     5101 TGGAAGTAAG GTGGTACGAT CGTGCCTTAT TAGGAAGGCA ACAGACGGGT CTGACATGGA
     5161 TTGGACGAAC CACTGAATTG CCGCATTGCA GAGATATTGT ATTTAAGTGC CTAGCTCGAT
     5221 ACATAAACGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG
     5281 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC
     5341 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA
     5401 TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA GGGAAACCAG AGGAGCTCTC
     5461 TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA GGCGAGGGGC GGCGACTGGT
     5521 GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG AGAGATGGGT GCGAGAGCGT
     5581 CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA ATTCGGTTAA GGCCAGGGGG
     5641 AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC AGGGAGCTAG AACGATTCGC
     5701 AGTTAATCCT GGCCTGTTAG AAACATCACG AAGGCTGTAG ACAAATACTG GGACAGCTAC
     5761 AACCATCCCT TCAGACAGGA TCAGAAGAAC TTACATCATT ATATAATACA GTAGCAACCC
     5821 TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA GGAAGCTTTA GACAAGATAG
     5881 AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGC GGCCGCTGAT CTTCAGACCT
     5941 GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA AGTAGTAAAA
     6001 ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGTGCA GAGAGAAAAA
     6061 AGAGCAGTGG GAATAGGAGC TTTGTTCCTT GGGTTCTTGG GAGCAGCAGG AAGCACTATG
     6121 GGCGCAGCGT CAATGACGCT GACGGTACAG GCCAGACAAT TATTGTCTGG TATAGTGCAG
     6181 CAGCAGAACA ATTTGCTGAG GGCTATTGAG GCGCAACAGC ATCTGTTGCA ACTCACAGTC
     6241 TGGGGCATCA AGCAGCTCCA GGCAAGAATC CTGGCTGTGG AAAGATACCT AAAGGATCAA
     6301 CAGCTCCTGG GGATTTGGGG TTGCTCTGGA AAACTCATTT GCACCACTGC TGTGCCTTGG
     6361 AATGCTAGTT GGAGTAATAA ATCTCTGGAA CAGATTTGGA ATCACACGAC CTGGATGGAG
     6421 TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA
     6481 AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG
     6541 AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAA TTATTCATAA TGATAGTAGG
     6601 AGGCTTGGTA GGTTTAAGAA TAGTTTTTGC TGTACTTTCT ATAGTGAATA GAGTTAGGCA
     6661 GGGATATTCA CCATTATCGT TTCAGACCCA CCTCCCAACC CCGAGGGGAC CCGACAGGCC
     6721 CGAAGGAATA GAAGAAGAAG GTGGAGAGAG AGACAGAGAC AGATCCATTC GATTAGTGAA
     6781 CGGATCTCGA CGGTATCGAT CACGAGACTA GCCTCGAGCG GCCGCCCCCT TCACCGAGGG
     6841 CCTATTTCCC ATGATTCCTT CATATTTGCA TATACGATAC AAGGCTGTTA GAGAGATAAT
     6901 TGGAATTAAT TTGACTGTAA ACACAAAGAT ATTAGTACAA AATACGTGAC GTAGAAAGTA
     6961 ATAATTTCTT GGGTAGTTTG CAGTTTTAAA ATTATGTTTT AAAATGGACT ATCATATGCT
     7021 TACCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA
     7081 CACCGG
//
