LOCUS       pEF6-hbeclin-1-D133AD149A        7095 bp    DNA     circular      8-AUG-2011
DEFINITION  LMBP 7301.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 7301
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 8-AUG-2011
            
            
COMMENT     VNTDATE|595008444|
COMMENT     VNTNAME|pEF6-hbeclin-1-D133AD149A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-hbeclin-1-D133AD149A" 0 7095 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BalI" "BglII" "KpnI" "NotI" "SacI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 2046 2097 1 7095 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1596 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2951 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BalI" "tggcca" 4 0 3 2042 0 2549 0 4227 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BglII" "agatct" 2 0 3 13 0 918 0 2097 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 1180 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 4 651 0 2602 0 3767 0 
COMMENT            6772 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "T7 promoter" 30 0 0 1546 1562 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1546..1562") 
COMMENT        #7=(CFSignal (CObList) "His-tag" 22 0 0 3022 3039 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3022..3039") 
COMMENT        #8=(CFSignal (CObList) "BGH polyA" 43 0 0 3068 3292 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3068..3292") 
COMMENT        #9=(CFSignal (CObList) "f1 ori" 33 0 0 3338 3766 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3338..3766") 
COMMENT        #10=(CFSignal (CObList) "blasticidin resistance" 4 0 0 4223 4621 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4223..4621") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4779 4853 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4779..4853") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4779 4853 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4779..4853)") 
COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 5167 5558 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5167..5558)") 
COMMENT        #14=(CFSignal (CObList) "SV40 early promoter" 29 0 0 3771 4052 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3771..4052") 
COMMENT        #15=(CFSignal (CObList) "SV40 ori" 33 0 0 4009 4084 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4009..4084") 
COMMENT        #16=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6099 6959 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(6099..6959)") 
COMMENT        #17=(CFSignal (CObList) "hEF1a promoter" 29 0 0 386 1529 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "386..1529") 
COMMENT        #18=(CFSignal (CObList) "Myc epitope" 22 0 0 2977 3006 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2977..3006") 
COMMENT        #19=(CFSignal (CObList) "synthetic EM7 promoter" 30 0 0 4157 4222 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4157..4222") 
COMMENT        #20=(CFSignal (CObList) "intron hEF1a promoter" 15 0 0 582 1520 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "582..1520") 
COMMENT        #21=(CFSignal (CObList) "lac promoter" 30 0 1 4992 5075 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4992..5075)") 
COMMENT        #22=(CFSignal (CObList) "D133A" 62 0 0 1993 1995 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1993..1995") 
COMMENT        #23=(CFSignal (CObList) "D149A" 62 0 0 2041 2043 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2041..2043") 
COMMENT        #24=(CFSignal (CObList) "hBECN1 mutant" 4 0 0 1597 2946 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1597..2946")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
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COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #26=(CFSignal (CObList) "Last modified: 8-AUG-2011" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pEF6-hbeclin-1-D133AD149A'" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 07/06/11 04:07" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 08/08/11 04:52" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7095 bp" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 13-JUL-2011" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #40=(CRefLinePar 
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COMMENT                   0 0) 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "LMBP 7301") 
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COMMENT                           (CObjectList 
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COMMENT                   "Annotations") 
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COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1597..2946" 1))) "hBECN1 mutant") 
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COMMENT                                        "Start: 4223  End: 4621" 1) 
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COMMENT                                   #55=(CLinePar 
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COMMENT                                 "blasticidin resistance") 
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COMMENT                                 "intron hEF1a promoter"))) "Intron (1 total)") 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2977..3006" 1))) "Myc epitope") 
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COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3022..3039" 1))) "His-tag"))) 
COMMENT                          "Misc. Marker (2 total)") 
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COMMENT                           (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                   #78=(CLinePar 
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COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3771..4052" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #83=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                   #86=(CLinePar 
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COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1546..1562" 1))) "T7 promoter") 
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COMMENT                                   #91=(CLinePar 
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COMMENT                                 "synthetic EM7 promoter") 
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COMMENT                                   #94=(CLinePar 
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COMMENT                                   #95=(CLinePar 
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COMMENT                                        "   complement(4992..5075)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (3 total)") 
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COMMENT                                  "pMB1 ori"))) "Replication Origin (3 total)") 
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COMMENT                                           "   complement(4779..4853)" 1))) 
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COMMENT                                           "   2041..2043" 1))) "D149A"))) 
COMMENT                           "Mutation (2 total)"))) "Feature Map") 
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COMMENT                                            " N1: 1596 " 1))))) "KpnI: 1 site") 
COMMENT                            5 1 "GGTACC" "CCATGG") 
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COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
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FEATURES             Location/Qualifiers
     promoter        1546..1562
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     3022..3039
                     /vntifkey="22"
                     /label=His-tag
     terminator      3068..3292
                     /vntifkey="43"
                     /label=BGH\polyA
     rep_origin      3338..3766
                     /vntifkey="33"
                     /label=f1\ori
     CDS             4223..4621
                     /vntifkey="4"
                     /label=blasticidin\resistance
     terminator      4779..4853
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4779..4853)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(5167..5558)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        3771..4052
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      4009..4084
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             complement(6099..6959)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        386..1529
                     /vntifkey="29"
                     /label=hEF1a\promoter
     misc_marker     2977..3006
                     /vntifkey="22"
                     /label=Myc\epitope
     promoter        4157..4222
                     /vntifkey="30"
                     /label=synthetic\EM7\promoter
     intron          582..1520
                     /vntifkey="15"
                     /label=intron\hEF1a\promoter
     promoter        complement(4992..5075)
                     /vntifkey="30"
                     /label=lac\promoter
     CDS             1597..2946
                     /vntifkey="4"
                     /label=hBECN1\mutant
     mutation        1993..1995
                     /vntifkey="62"
                     /label=D133A
                     /note="WT: GAT"
     mutation        2041..2043
                     /vntifkey="62"
                     /label=D149A
                     /note="WT: GAC"
BASE COUNT     1667 a      1768 c      1902 g      1756 t         2 others
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 
     1561 tagggagacc caagctggct aggtaagctt ggtaccatgg aagggtctaa gacgtccaac 
     1621 aacagcacca tgcaggtgag cttcgtgtgc cagcgctgca gccagcccct gaaactggac 
     1681 acgagtttca agatcctgga ccgtgtcacc atccaggaac tcacagctcc attacttacc 
     1741 acagcccagg cgaaaccagg agagacccag gaggaagaga ctaactcagg agaggagcca 
     1801 tttattgaaa ctcctcgcca ggatggtgtc tctcgcagat tcatcccccc agccaggatg 
     1861 atgtccacag aaagtgccaa cagcttcact ctgattgggg aggcatctga tggcggcacc 
     1921 atggagaacc tcagccgaag actgaaggtc actggggacc tttttgacat catgtcgggc 
     1981 cagacagatg tggctcaccc actctgtgag gaatgcacag atactctttt agaccagctg 
     2041 gccactcagc tcaacgtcac tgaaaatgag tgtcagaact acaaacgctg tttggagatc 
     2101 ttagagcaaa tgaatgagga tgacagtgaa cagttacaga tggagctaaa ggagctggca 
     2161 ctagaggagg agaggctgat ccaggagctg gaagacgtgg aaaagaaccg caagatagtg 
     2221 gcagaaaatc tcgagaaggt ccaggctgag gctgagagac tggatcagga ggaagctcag 
     2281 tatcagagag aatacagtga atttaaacga cagcagctgg agctggatga tgagctgaag 
     2341 agtgttgaaa accagatgcg ttatgcccag acgcagctgg ataagctgaa gaaaaccaac 
     2401 gtctttaatg caaccttcca catctggcac agtggacagt ttggcacaat caataacttc 
     2461 aggctgggtc gcctgcccag tgttcccgtg gaatggaatg agattaatgc tgcttggggc 
     2521 cagactgtgt tgctgctcca tgctctggcc aataagatgg gtctgaaatt tcagagatac 
     2581 cgacttgttc cttacggaaa ccattcatat ctggagtctc tgacagacaa atctaaggag 
     2641 ctgccgttat actgttctgg ggggttgcgg tttttctggg acaacaagtt tgaccatgca 
     2701 atggtggctt tcctggactg tgtgcagcag ttcaaagaag aggttgagaa aggcgagact 
     2761 cgtttttgtc ttccctacag gatggatgtg gagaaaggca agattgaaga cacaggaggc 
     2821 agtggcggct cctattccat caaaacccag tttaactctg aggagcagtg gacaaaagct 
     2881 ctcaagttca tgctgacgaa tcttaagtgg ggtcttgctt gggtgtcctc acaattttat 
     2941 aacaaanngc ggccgctcga gtctagaggg cccttcgaac aaaaactcat ctcagaagag 
     3001 gatctgaata tgcataccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca 
     3061 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 
     3121 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 
     3181 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 
     3241 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 
     3301 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 
     3361 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 
     3421 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 
     3481 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 
     3541 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 
     3601 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 
     3661 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 
     3721 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 
     3781 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 
     3841 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 
     3901 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 
     3961 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 
     4021 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 
     4081 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 
     4141 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata 
     4201 cgacaaggtg aggaactaaa ccatggccaa gcctttgtct caagaagaat ccaccctcat 
     4261 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag 
     4321 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg 
     4381 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct 
     4441 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg 
     4501 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg 
     4561 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta 
     4621 agcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg 
     4681 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 
     4741 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata 
     4801 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 
     4861 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga 
     4921 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 
     4981 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 
     5041 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 
     5101 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     5161 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     5221 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 
     5281 caggaaagaa catgtgagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     5341 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     5401 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     5461 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     5521 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     5581 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     5641 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     5701 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     5761 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     5821 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 
     5881 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     5941 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     6001 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 
     6061 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 
     6121 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 
     6181 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 
     6241 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 
     6301 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 
     6361 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 
     6421 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 
     6481 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 
     6541 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 
     6601 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 
     6661 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 
     6721 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 
     6781 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 
     6841 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 
     6901 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 
     6961 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 
     7021 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 
     7081 gtgccacctg acgtc 
//
