LOCUS       pEF1a-E-hA20-dPEST-IRES-DsRed        8449 bp    DNA     circular     23-SEP-2011
DEFINITION  LMBP 7411.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|602011313|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEF1a-E-hA20-dPEST-IRES-DsRed|
COMMENT     VNTAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     misc_feature    2266..2373
                     /vntifkey="21"
                     /label=ZF7
     misc_feature    2128..2244
                     /vntifkey="21"
                     /label=ZF6
     misc_feature    1951..2067
                     /vntifkey="21"
                     /label=ZF5
     misc_feature    1801..1917
                     /vntifkey="21"
                     /label=ZF4
     misc_feature    1471..1578
                     /vntifkey="21"
                     /label=ZF2
     misc_feature    1198..1314
                     /vntifkey="21"
                     /label=ZF1
     misc_feature    2083..2187
                     /vntifkey="21"
                     /label=region\corresponding\to\p75/p30\MALT1\cleavage\site
     misc_feature    1486..1497
                     /vntifkey="21"
                     /label=potential\p55\MALT1\cleavage\site
     misc_feature    1363..1380
                     /vntifkey="21"
                     /label=p50\MALT1\cleavage\site
     misc_feature    2377..2420
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the human A20 gene"
     CDS             61..2376
                     /vntifkey="4"
                     /label=hA20
                     /note="human A20 coding sequence"
     CDS             13..51
                     /vntifkey="4"
                     /label=E-tag
                     /note="E-tag"
     rep_origin      6338..6981
                     /vntifkey="33"
                     /label=pUC\origin
     polyA_signal    5989..6007
                     /vntifkey="25"
                     /label=HSV\TK\polyA\signal
     CDS             4959..5753
                     /vntifkey="4"
                     /label=Kan/NeoR
     rep_origin      4775..4910
                     /vntifkey="33"
                     /label=SV40\origin
     promoter        4608..4876
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      complement(3979..4434)
                     /vntifkey="33"
                     /label=f1\ori
     polyA_signal    3882..3932
                     /vntifkey="25"
                     /label=SV40\early\polyA\signal
     misc_feature    3727..3729
                     /vntifkey="21"
                     /label=STOP
     CDS             3052..3726
                     /vntifkey="4"
                     /label=DsRed-Express2
     misc_binding    2464..3051
                     /vntifkey="20"
                     /label=IRES2
     promoter        7075..8409
                     /vntifkey="29"
                     /label=pEF1-alpha
     misc_feature    352..831
                     /vntifkey="21"
                     /label=OTU\domain
BASE COUNT     1971 a      2303 c      2307 g      1868 t 
ORIGIN
        1 aattccacca tgggtgcgcc ggtgccgtat ccggacccgc tggaaccgcg tgcggccgca 
       61 atggctgaac aagtccttcc tcaggctttg tatttgagca atatgcggaa agctgtgaag 
      121 atacgggaga gaactccaga agacattttt aaacctacta atgggatcat tcatcatttt 
      181 aaaaccatgc accgatacac actggaaatg ttcagaactt gccagttttg tcctcagttt 
      241 cgggagatca tccacaaagc cctcatcgac agaaacatcc aggccaccct ggaaagccag 
      301 aagaaactca actggtgtcg agaagtccgg aagcttgtgg cgctgaaaac gaacggtgac 
      361 ggcaattgcc tcatgcatgc cacttctcag tacatgtggg gcgttcagga cacagacttg 
      421 gtactgagga aggcgctgtt cagcacgctc aaggaaacag acacacgcaa ctttaaattc 
      481 cgctggcaac tggagtctct caaatctcag gaatttgttg aaacggggct ttgctatgat 
      541 actcggaact ggaatgatga atgggacaat cttatcaaaa tggcttccac agacacaccc 
      601 atggcccgaa gtggacttca gtacaactca ctggaagaaa tacacatatt tgtcctttgc 
      661 aacatcctca gaaggccaat cattgtcatt tcagacaaaa tgctaagaag tttggaatca 
      721 ggttccaatt tcgccccttt gaaagtgggt ggaatttact tgcctctcca ctggcctgcc 
      781 caggaatgct acagataccc cattgttctc ggctatgaca gccatcattt tgtacccttg 
      841 gtgaccctga aggacagtgg gcctgaaatc cgagctgttc cacttgttaa cagagaccgg 
      901 ggaagatttg aagacttaaa agttcacttt ttgacagatc ctgaaaatga gatgaaggag 
      961 aagctcttaa aagagtactt aatggtgata gaaatccccg tccaaggctg ggaccatggc 
     1021 acaactcatc tcatcaatgc cgcaaagttg gatgaagcta acttaccaaa agaaatcaat 
     1081 ctggtagatg attactttga acttgttcag catgagtaca agaaatggca ggaaaacagc 
     1141 gagcagggga ggagagaggg gcacgcccag aatcccatgg aaccttccgt gccccagctt 
     1201 tctctcatgg atgtaaaatg tgaaacgccc aactgcccct tcttcatgtc tgtgaacacc 
     1261 cagcctttat gccatgagtg ctcagagagg cggcaaaaga atcaaaacaa actcccaaag 
     1321 ctgaactcca agccgggccc tgaggggctc cctggcatgg cgctcggggc ctctcgggga 
     1381 gaagcctatg agcccttggc gtggaaccct gaggagtcca ctggggggcc tcattcggcc 
     1441 ccaccgacag cacccagccc ttttctgttc agtgagacca ctgccatgaa gtgcaggagc 
     1501 cccggctgcc ccttcacact gaatgtgcag cacaacggat tttgtgaacg ttgccacaac 
     1561 gcccggcaac ttcacgccag ccacgcccca gaccacacaa ggcacttgga tcccgggaag 
     1621 tgccaagcct gcctccagga tgttaccagg acatttaatg ggatctgcag tacttgcttc 
     1681 caccagcgtt ccaagtcaga tccctcgcgg ctcgtccgga gcccctcccc gcattcttgc 
     1741 cacagagctg gaaacgacgc ccctgctggc tgcctgtctc aagctgcacg gactcctggg 
     1801 gacaggacgg ggacgagcaa gtgcagaaaa gccggctgcg tgtattttgg gactccagaa 
     1861 aacaagggct tttgcacact gtgtttcatc gagtacagag aaaacaaaca ttttgctgct 
     1921 gcctcaggga aagtcagtcc cacagcgtcc aggttccaga acaccattcc gtgcctgggg 
     1981 agggaatgcg gcacccttgg aagcaccatg tttgaaggat actgccagaa gtgtttcatt 
     2041 gaagctcaga atcagagatt tcatgaggcc aaaaggacag aagagcaact gagatcgagc 
     2101 cagcgcagag atgtgcctcg aaccacacaa agcacctcaa ggcccaagtg cgcccgggcc 
     2161 tcctgcaaga acatcctggc ctgccgcagc gaggagctct gcatggagtg tcagcatccc 
     2221 aaccagagga tgggccctgg ggcccaccgg ggtgagcctg cccccgaaga cccccccaag 
     2281 cagcgttgcc gggcccccgc ctgtgatcat tttggcaatg ccaagtgcaa cggctactgc 
     2341 aacgaatgct ttcagttcaa gcagatgtat ggctaaccgg aaacaggtgg gtcacctcct 
     2401 gcaagaagtg gggcctcgag gtcgaggaat tctgcagtcg acggtaccgc gggcccggga 
     2461 tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc 
     2521 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg 
     2581 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca 
     2641 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa 
     2701 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt 
     2761 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt 
     2821 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca 
     2881 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc 
     2941 ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc 
     3001 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggatagc 
     3061 actgagaacg tcatcaagcc cttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 
     3121 ggccacgagt tcgagatcga gggcgagggc gagggcaagc cctacgaggg cacccagacc 
     3181 gccaagctgc aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc 
     3241 cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag 
     3301 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 
     3361 gtggtgaccg tgacccagga ctcctccctg caggacggca ccttcatcta ccacgtgaag 
     3421 ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc agaagaagac tctgggctgg 
     3481 gagccctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccacaag 
     3541 gcgctgaagc tgaagggcgg cggccactac ctggtggagt tcaagtcaat ctacatggcc 
     3601 aagaagcccg tgaagctgcc cggctactac tacgtggact ccaagctgga catcacctcc 
     3661 cacaacgagg actacaccgt ggtggagcag tacgagcgcg ccgaggcccg ccaccacctg 
     3721 ttccagtagg cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt 
     3781 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 
     3841 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 
     3901 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 
     3961 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt 
     4021 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat 
     4081 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 
     4141 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 
     4201 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc 
     4261 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 
     4321 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 
     4381 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg 
     4441 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 
     4501 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 
     4561 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc 
     4621 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 
     4681 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 
     4741 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 
     4801 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 
     4861 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 
     4921 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 
     4981 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 
     5041 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 
     5101 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat 
     5161 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 
     5221 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 
     5281 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 
     5341 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 
     5401 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 
     5461 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc 
     5521 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 
     5581 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 
     5641 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 
     5701 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 
     5761 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 
     5821 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 
     5881 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac 
     5941 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca 
     6001 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg 
     6061 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt 
     6121 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc 
     6181 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca 
     6241 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 
     6301 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 
     6361 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 
     6421 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 
     6481 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 
     6541 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 
     6601 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 
     6661 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 
     6721 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 
     6781 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 
     6841 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 
     6901 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 
     6961 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 
     7021 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatgagtaa 
     7081 ttcatacaaa aggactcgcc cctgccttgg ggaatcccag ggaccgtcgt taaactccca 
     7141 ctaacgtaga acccagagat cgctgcgttc ccgccccctc acccgcccgc tctcgtcatc 
     7201 actgaggtgg agaagagcat gcgtgaggct ccggtgcccg tcagtgggca gagcgcacat 
     7261 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa 
     7321 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 
     7381 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 
     7441 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg 
     7501 gttatggccc ttgcgtgcct tgaattactt ccacgcccct ggctgcagta cgtgattctt 
     7561 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc 
     7621 ccttcgcctc gtgcttgagt tgaggcctgg cttgggcgct ggggccgccg cgtgcgaatc 
     7681 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt 
     7741 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat 
     7801 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag 
     7861 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag 
     7921 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc 
     7981 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc 
     8041 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag 
     8101 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg 
     8161 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct 
     8221 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact 
     8281 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt 
     8341 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag 
     8401 gtgtcgtgac gctagcgcta ccggactcag atctcgagct caagcttcg 
//
