LOCUS       pEF6-F-GFP-S65T-DEVDG-QP-IRES-DsRed        8104 bp    DNA     circular      7-OCT-2011
DEFINITION  LMBP 7430.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|603388115|
COMMENT     VNTDBDATE|603388115|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEF6-F-GFP-S65T-DEVDG-QP-IRES-DsRed|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-F-GFP-S65T-DEVDG-QP-IRES-DsRed" 0 8104 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 2 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AarI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" "AclI" 
COMMENT         "AcuI" "AfeI" "AflII" "AflIII" "AgeI" "AhdI" "AleI" "AloI" "AluI" 
COMMENT         "AlwI" "AlwNI" "ApaBI" "ApaI" "ApaLI" "ApoI" "AscI" "AseI" "AsiSI" 
COMMENT         "AvaI" "AvaII" "AvrII" "BaeI" "BamHI" "BanI" "BanII" "BbeI" "Bbr7I" 
COMMENT         "BbsI" "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BcgI" "BciVI" "BclI" 
COMMENT         "BfaI" "BfrBI" "BglI" "BglII" "BlpI" "Bme1580I" "BmgBI" "BmrI" "BmtI" 
COMMENT         "BplI" "BpmI" "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" "BsaJI" 
COMMENT         "BsaWI" "BsaXI" "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiEI" 
COMMENT         "BsiHKAI" "BsiWI" "BslI" "BsmAI" "BsmBI" "BsmFI" "BsmI" "Bsp1286I" 
COMMENT         "Bsp24I" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" 
COMMENT         "BsrGI" "BsrI" "BssHII" "BssSI" "BstAPI" "BstBI" "BstEII" "BstF5I" 
COMMENT         "BstKTI" "BstNI" "BstUI" "BstXI" "BstYI" "BstZ17I" "Bsu36I" "BtgI" 
COMMENT         "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "CjeI" "CjePI" "ClaI" "Csp6I" 
COMMENT         "CviAII" "CviJI" "DdeI" "DpnI" "DraI" "DraIII" "DrdI" "EaeI" "EagI" 
COMMENT         "EarI" "EciI" "Eco57MI" "EcoHI" "EcoICRI" "EcoNI" "EcoO109I" "EcoRI" 
COMMENT         "EcoRV" "EsaBC3I" "FalI" "FatI" "FauI" "FmuI" "Fnu4HI" "FokI" "FseI" 
COMMENT         "FspAI" "FspI" "GdiII" "HaeI" "HaeII" "HaeIII" "HaeIV" "HgaI" "HhaI" 
COMMENT         "Hin4I" "HincII" "HindIII" "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" 
COMMENT         "Hpy188I" "Hpy188III" "Hpy8I" "Hpy99I" "HpyAV" "HpyCH4I" "HpyCH4III" 
COMMENT         "HpyCH4IV" "HpyCH4V" "HpyF10VI" "KasI" "KpnI" "LpnI" "MaeIII" "MboI" 
COMMENT         "MboII" "MfeI" "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MslI" 
COMMENT         "MspA1I" "MwoI" "NaeI" "NarI" "NciI" "NcoI" "NdeI" "NgoMIV" "NheI" 
COMMENT         "NlaIII" "NlaIV" "Nli3877I" "NotI" "NruI" "NsiI" "NspI" "PacI" "PciI" 
COMMENT         "PflMI" "PfoI" "PleI" "PmeI" "PmlI" "PpiI" "Ppu10I" "PpuMI" "PshAI" 
COMMENT         "PsiI" "Psp03I" "PspGI" "PspOMI" "PsrI" "PssI" "PstI" "PvuI" "PvuII" 
COMMENT         "RleAI" "RsaI" "RsrII" "SacI" "SacII" "SalI" "SanDI" "SapI" "Sau96I" 
COMMENT         "SbfI" "ScaI" "SciI" "ScrFI" "SelI" "SexAI" "SfaNI" "SfcI" "SfiI" 
COMMENT         "SfoI" "SgrAI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" "SphI" "SrfI" 
COMMENT         "Sse232I" "Sse8647I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "StyD4I" 
COMMENT         "StyI" "SwaI" "TaiI" "TaqI" "TaqII" "TatI" "TauI" "TfiI" "TseI" 
COMMENT         "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "Tth111I" "Tth111II" 
COMMENT         "UnbI" "UthSI" "VpaK11AI" "XbaI" "XcmI" "XhoI" "XmaI" "XmnI" "ZraI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 8104 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AarI" "cacctgc" 12 16 2 3010 0 3386 1 0 "") 
COMMENT        #1=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 8104 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 4 1592 0 1681 0 2649 0 
COMMENT            3112 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AccI" "gtmkac" 3 0 4 2136 0 5920 0 5927 0 8104 
COMMENT            0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AceIII" "cagctc" 14 18 4 4734 1 5345 0 6252 0 
COMMENT            7481 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "AciI" "ccgc" 2 4 89 62 0 452 1 489 0 569 0 603 
COMMENT            0 605 1 786 0 789 0 910 1 950 0 952 1 956 1 999 1 1014 1 1081 0 1096 
COMMENT            0 1105 1 1141 1 1153 0 1194 1 1209 1 1213 1 2469 0 2548 1 2552 0 
COMMENT            2653 0 2655 1 2668 0 2710 0 2941 1 2973 1 3011 1 3385 0 3409 1 3484 
COMMENT            0 3562 1 3713 0 3763 1 3766 1 3914 0 3936 1 3940 0 4061 0 4287 1 
COMMENT            4311 1 4360 1 4374 1 4377 1 4405 0 4432 0 4952 0 4964 0 4973 0 4985 
COMMENT            0 4995 0 5006 0 5052 0 5352 0 5434 1 5501 1 5685 0 5688 0 5754 1 
COMMENT            5990 0 6093 0 6149 1 6159 1 6183 0 6226 1 6233 1 6254 1 6334 0 6362 
COMMENT            0 6489 0 6508 0 6629 0 6739 1 6874 0 6883 1 7245 0 7336 0 7527 1 
COMMENT            7573 0 7694 1 7738 0 7815 0 7924 0 8023 1 8070 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "AclI" "aacgtt" 3 0 4 545 0 2694 0 7408 0 7781 
COMMENT            0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 9 1421 0 2037 0 3116 0 
COMMENT            3747 0 3771 0 3777 0 5330 0 6837 0 7849 1 0 "") 
COMMENT        #8=(CRSite (CStringList) "AfeI" "agcgct" 4 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AflII" "cttaag" 2 0 2 731 0 1643 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AflIII" "acrygt" 2 0 8 229 0 985 0 2009 0 
COMMENT             2986 0 3161 0 5163 0 5659 0 6300 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AgeI" "accggt" 2 0 2 430 0 2615 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 2 3437 0 7182 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 1 3885 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AloI" "gaacnnnnnntcc" 26 21 2 3662 0 4617 1 0 
COMMENT             "") 
COMMENT        #15=(CRSite (CStringList) "AluI" "agct" 3 0 42 136 0 268 0 314 0 690 0 
COMMENT             1048 0 1178 0 1322 0 1414 0 1538 0 1575 0 1588 0 1600 0 1648 0 2363 
COMMENT             0 2897 0 3392 0 3509 0 3755 0 3821 0 3851 0 4324 0 4491 0 4748 0 
COMMENT             5068 0 5122 0 5132 0 5335 0 5440 0 5804 0 5937 0 5943 0 5965 0 6060 
COMMENT             0 6124 0 6242 0 6457 0 6547 0 6593 0 6850 0 7371 0 7471 0 7534 0 0 
COMMENT             "") 
COMMENT        #16=(CRSite (CStringList) "AlwI" "ggatc" 10 11 25 13 0 16 1 676 1 1470 0 
COMMENT             1599 1 1612 0 2309 1 2492 0 2607 0 2659 1 2672 0 3136 0 3731 1 5158 
COMMENT             0 5548 0 5737 1 5766 0 6850 1 6936 1 6938 0 7034 1 7035 0 7498 1 
COMMENT             7815 0 7819 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 3 81 0 2366 0 6705 0 0 
COMMENT             "") 
COMMENT        #18=(CRSite (CStringList) "ApaBI" "gcannnnntgc" 9 0 3 1133 0 4850 0 4922 
COMMENT             0 0 "") 
COMMENT        #19=(CRSite (CStringList) "ApaI" "gggccc" 6 0 5 969 0 2529 0 2571 0 2661 
COMMENT             0 2788 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 4 33 0 3303 0 6603 0 7849 
COMMENT             0 0 "") 
COMMENT        #21=(CRSite (CStringList) "ApoI" "raatty" 2 0 8 128 0 855 0 1441 0 1627 
COMMENT             0 1763 0 1820 0 4761 0 5839 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AseI" "attaat" 3 0 5 4775 0 5175 0 6071 0 
COMMENT             6130 0 7354 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 9 397 0 499 0 685 0 1200 0 
COMMENT             1317 0 2555 0 2660 0 3519 0 5125 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 5 320 0 2341 0 5392 0 7320 
COMMENT             0 7542 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AvrII" "cctagg" 2 0 2 2822 0 5104 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 1 2028 1 0 "") 
COMMENT        #29=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 1604 0 2664 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 19 358 0 433 0 806 0 1065 
COMMENT             0 1125 0 1290 0 1301 0 1592 0 1681 0 2649 0 2964 0 3112 0 3375 0 
COMMENT             3603 0 4143 0 4537 0 5507 0 6044 0 7130 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "BanII" "grgcyc" 6 0 14 741 0 969 0 1180 0 
COMMENT             1602 0 2529 0 2571 0 2661 0 2788 0 3318 0 3691 0 4296 0 4332 0 4507 
COMMENT             0 5497 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 1 1294 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 5 2792 1 2916 0 3683 0 
COMMENT             4272 0 5323 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbsI" "gaagac" 9 13 5 2797 1 2911 0 3678 0 
COMMENT             4267 0 5318 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 1 1254 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "BbvI" "gcagc" 14 18 27 188 1 323 0 650 1 813 
COMMENT             1 858 1 1155 1 2350 1 2950 0 3379 1 3808 1 4408 0 5344 0 5412 1 
COMMENT             5415 1 5418 1 5449 0 5588 1 5813 0 6111 1 6192 1 6210 1 6618 1 6708 
COMMENT             0 6711 0 6917 0 7220 1 7611 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "BccI" "ccatc" 10 11 19 272 1 1142 1 1362 1 
COMMENT             1736 1 2078 1 2198 1 2249 1 2405 1 2426 1 2637 0 4106 0 4563 1 4595 
COMMENT             0 4976 0 5311 0 5560 1 7226 0 7350 0 7637 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BceAI" "acggc" 18 20 18 523 1 574 1 1068 1 
COMMENT             1243 1 1278 1 1940 0 2749 1 3341 0 3473 0 3575 0 3617 0 3668 0 3734 
COMMENT             0 4551 0 5297 0 5364 0 5594 0 6791 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BcefI" "acggc" 18 19 18 524 1 575 1 1069 1 
COMMENT             1244 1 1279 1 1940 0 2750 1 3341 0 3473 0 3575 0 3617 0 3668 0 3734 
COMMENT             0 4551 0 5297 0 5364 0 5594 0 6791 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 1 7721 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 3 4351 0 6492 1 8019 1 
COMMENT             0 "") 
COMMENT        #42=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 4065 0 5155 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BfaI" "ctag" 2 0 23 251 0 327 0 438 0 844 0 
COMMENT             1580 0 1611 0 2219 0 2418 0 2562 0 2823 0 3191 0 3948 0 4087 0 4333 
COMMENT             0 4425 0 5105 0 5342 0 5874 0 5934 0 5938 0 6784 0 7037 0 7372 0 0 
COMMENT             "") 
COMMENT        #44=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 3 2611 0 4852 0 4924 0 0 
COMMENT             "") 
COMMENT        #45=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 5 1123 0 1299 0 1416 
COMMENT             0 2973 0 7302 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BglII" "agatct" 2 0 2 13 0 918 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 1 5628 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "Bme1580I" "gkgcmc" 6 0 13 37 0 363 0 969 0 
COMMENT             1761 0 2529 0 2571 0 2661 0 2788 0 3307 0 3378 0 5422 0 6607 0 7853 
COMMENT             0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 1 3214 1 0 "") 
COMMENT        #50=(CRSite (CStringList) "BmrI" "actggg" 12 11 6 471 0 3016 1 3440 1 
COMMENT             4983 1 5396 0 7222 1 0 "") 
COMMENT        #51=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 2421 0 0 "") 
COMMENT        #52=(CRSite (CStringList) "BplI" "gagnnnnnctc" 25 0 1 5071 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "BpmI" "ctggag" 23 21 4 1734 0 5732 1 5789 0 
COMMENT             7252 1 0 "") 
COMMENT        #54=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 2 181 1 1254 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 11 776 0 1028 1 1462 1 
COMMENT             2369 0 3058 1 5234 1 5511 0 6380 1 6678 0 6919 1 7787 1 0 "") 
COMMENT        #56=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 9 672 0 2012 0 2987 0 
COMMENT             3473 0 3620 0 3839 0 4578 0 5164 0 5662 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 2 3955 0 5531 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 4 1291 0 5730 0 7719 0 
COMMENT             8101 0 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 3 1560 1 2325 1 7243 1 0 
COMMENT             "") 
COMMENT        #60=(CRSite (CStringList) "BsaJI" "ccnngg" 2 0 35 500 0 603 0 772 0 950 
COMMENT             0 1099 0 1100 0 1277 0 1436 0 1653 0 1853 0 2653 0 2660 0 2822 0 
COMMENT             3143 0 3205 0 3256 0 3403 0 3427 0 3463 0 3520 0 3580 0 3883 0 3907 
COMMENT             0 4135 0 4816 0 4888 0 5011 0 5046 0 5104 0 5125 0 5231 0 5536 0 
COMMENT             5643 0 6039 0 6449 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BsaWI" "wccggw" 2 0 6 355 0 430 0 2615 0 6495 
COMMENT             0 6642 0 7473 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 6 3660 0 3774 1 
COMMENT             3858 0 3876 0 4617 1 6145 1 0 "") 
COMMENT        #63=(CRSite (CStringList) "BscAI" "gcatc" 10 12 16 51 1 1786 1 3094 1 
COMMENT             4193 0 4277 1 4860 0 4932 0 5305 0 5362 0 5494 0 5540 0 5840 0 6385 
COMMENT             0 7437 0 7632 1 7877 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BseMII" "ctcag" 16 14 14 54 0 101 1 1268 0 
COMMENT             1376 1 1498 0 2604 0 3259 1 4188 1 4297 1 5055 1 6578 0 6987 0 7153 
COMMENT             0 7670 1 0 "") 
COMMENT        #65=(CRSite (CStringList) "BseRI" "gaggag" 17 15 4 3061 1 3578 1 5100 0 
COMMENT             5660 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "BseYI" "cccagc" 2 6 10 779 1 977 0 2366 1 
COMMENT             3682 1 4278 1 4324 1 4825 0 4897 0 5415 1 6593 1 0 "") 
COMMENT        #67=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 3 548 0 907 1 5420 0 0 
COMMENT             "") 
COMMENT        #68=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 9 1123 0 2552 0 3940 0 
COMMENT             5352 0 5468 0 6216 0 6629 0 7552 0 7701 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 7 37 0 1180 0 1602 0 
COMMENT             3307 0 6607 0 7768 0 7853 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 35 368 0 369 0 443 0 
COMMENT             505 0 506 0 621 0 692 0 773 0 1027 0 1100 0 1101 0 1159 0 1171 0 
COMMENT             1232 0 2771 0 2934 0 2954 0 3124 0 3206 0 3372 0 3427 0 3651 0 3717 
COMMENT             0 3776 0 3872 0 3935 0 4359 0 4685 0 5012 0 5504 0 5755 0 6148 0 
COMMENT             6329 0 6495 0 6774 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 9 826 0 845 0 1046 0 1390 
COMMENT             1 1560 1 2325 1 5252 0 7243 1 8019 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BsmFI" "gggac" 16 20 11 379 1 960 1 1706 1 
COMMENT             3224 0 3425 1 3444 0 4798 1 4870 1 4934 1 5405 0 5741 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 4 2817 1 2856 0 4044 0 
COMMENT             5869 1 0 "") 
COMMENT        #76=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 23 37 0 363 0 741 0 
COMMENT             969 0 1180 0 1602 0 1761 0 2529 0 2571 0 2661 0 2788 0 3307 0 3318 
COMMENT             0 3378 0 3691 0 4296 0 4332 0 4507 0 5422 0 5497 0 6607 0 7768 0 
COMMENT             7853 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "Bsp24I" "gacnnnnnntgg" 25 20 8 1591 0 1851 1 
COMMENT             2123 0 2273 1 5600 0 5752 0 6992 0 8086 1 0 "") 
COMMENT        #78=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 14 53 0 102 1 1267 0 
COMMENT             1377 1 1497 0 2603 0 3260 1 4189 1 4298 1 5056 1 6577 0 6986 0 7152 
COMMENT             0 7671 1 0 "") 
COMMENT        #79=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "BspHI" "tcatga" 2 0 2 7009 0 8017 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "BspMI" "acctgc" 11 15 3 876 0 3010 0 3386 1 0 
COMMENT             "") 
COMMENT        #82=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 7 1141 1 1209 1 2554 0 
COMMENT             4434 0 5992 0 6233 1 8023 1 0 "") 
COMMENT        #83=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 3 2782 0 7243 0 7417 1 0 
COMMENT             "") 
COMMENT        #84=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 7 430 0 1052 0 2615 0 
COMMENT             2724 0 4473 0 5732 0 7262 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 1963 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "BsrI" "actgg" 7 5 26 75 1 466 0 487 0 933 0 
COMMENT             1129 1 1616 1 1717 0 1840 0 2704 0 3022 1 3446 1 3452 1 3929 1 4667 
COMMENT             0 4989 1 5367 0 5391 0 6100 1 6697 0 6710 0 6822 1 7228 1 7346 1 
COMMENT             7389 1 7658 0 7828 1 0 "") 
COMMENT        #87=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 1 3902 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "BssSI" "cacgag" 2 6 5 749 1 3330 0 5407 1 
COMMENT             6462 1 7846 1 0 "") 
COMMENT        #89=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 3 1132 0 4849 0 
COMMENT             4921 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 2 2309 0 2574 0 0 "") 
COMMENT        #91=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 2 3398 0 3569 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 12 2392 0 3106 0 3433 1 
COMMENT             3490 1 4289 0 4968 1 5297 1 5532 1 5745 0 7161 1 7342 1 7629 1 0 "") 
COMMENT        #93=(CRSite (CStringList) "BstKTI" "gatc" 4 0 39 8 0 16 0 24 0 684 0 921 
COMMENT             0 1465 0 1607 0 1915 0 2317 0 2487 0 2515 0 2602 0 2667 0 3131 0 
COMMENT             3136 0 3343 0 3739 0 3953 0 4068 0 5153 0 5158 0 5468 0 5529 0 5543 
COMMENT             0 5745 0 5761 0 6858 0 6933 0 6944 0 6952 0 7030 0 7042 0 7147 0 
COMMENT             7488 0 7506 0 7552 0 7810 0 7827 0 7863 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 21 611 0 660 0 768 0 773 0 
COMMENT             1070 0 1101 0 1299 0 2796 0 2955 0 3428 0 3582 0 3777 0 4137 0 4818 
COMMENT             0 4873 0 4890 0 5537 0 6040 0 6317 0 6438 0 6451 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "BstUI" "cgcg" 3 0 30 121 0 209 0 231 0 452 0 
COMMENT             605 0 791 0 815 0 952 0 1014 0 1078 0 1194 0 2471 0 2655 0 3541 0 
COMMENT             3715 0 3904 0 3942 0 4350 0 4374 0 4394 0 4770 0 5464 0 5754 0 6147 
COMMENT             0 6149 0 6336 0 6917 0 7247 0 7740 0 8072 0 0 "") 
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COMMENT                              (CGroupPar (CParagraph 34268264 (8 0) 1 1 1 0 178) 
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COMMENT                              (CGroupPar (CParagraph 25109840 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CjeI: 26 sites") 26 20 
COMMENT                             "CCANNNNNNGTNNNNNNNNNNNNNNNN" 
COMMENT                             "GGTNNNNNNCANNNNNNNNNNNNNNNN") 
COMMENT                       #569=(CRSFolderPar 
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COMMENT                              "FalI: 2 sites") 38 1 
COMMENT                             "NNNNNNNNNNNNNNAAGNNNNNCTTNNNNNNNNNNNNNN" 
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COMMENT                             "GGATGNNNNNNNNNNNNNN" "CCTACNNNNNNNNNNNNNN") 
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COMMENT                                              191) " N1: 229 " 1))))) 
COMMENT                              "MluI: 1 site") 1 5 "ACGCGT" "TGCGCA") 
COMMENT                       #654=(CRSFolderPar 
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COMMENT                              (CGroupPar (CParagraph 25165136 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MlyI: 14 sites") 10 10 
COMMENT                             "GAGTCNNNNNN" "CTCAGNNNNNN") 
COMMENT                       #655=(CRSFolderPar 
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COMMENT                              (CGroupPar (CParagraph 25165168 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MmeI: 7 sites") 26 24 
COMMENT                             "TCCRACNNNNNNNNNNNNNNNNNNNNN" 
COMMENT                             "AGGYTGNNNNNNNNNNNNNNNNNNNNN") 
COMMENT                       #656=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25165200 (8 0) 1 1 1 0 178) 
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COMMENT                             "CCTCNNNNNNNN" "GGAGNNNNNNNN") 
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COMMENT                              (CGroupPar (CParagraph 25165232 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MscI: 4 sites") 3 3 "TGGCCA" 
COMMENT                             "ACCGGT") 
COMMENT                       #658=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25165264 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MseI: 32 sites") 1 3 "TTAA" 
COMMENT                             "AATT") 
COMMENT                       #659=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25165296 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MslI: 6 sites") 5 5 "CAYNNNNRTG" 
COMMENT                             "GTRNNNNYAC") 
COMMENT                       #660=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25165328 (8 0) 1 1 1 0 178) 
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COMMENT                             "GKCGMC") 
COMMENT                       #661=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25165360 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MwoI: 49 sites") 7 4 
COMMENT                             "GCNNNNNNNGC" "CGNNNNNNNCG") 
COMMENT                       #662=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25165392 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "NaeI: 3 sites") 3 3 "GCCGGC" 
COMMENT                             "CGGCCG") 
COMMENT                       #663=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25165424 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CObjectList 
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COMMENT                                              191) " N1: 1291 " 1))))) 
COMMENT                              "NarI: 1 site") 2 4 "GGCGCC" "CCGCGG") 
COMMENT                       #666=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25165592 (8 0) 1 1 1 0 178) 
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COMMENT                             "GGSCC") 
COMMENT                       #667=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25165624 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "NcoI: 5 sites") 1 5 "CCATGG" 
COMMENT                             "GGTACC") 
COMMENT                       #668=(CRSFolderPar 
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COMMENT                              "NdeI: 1 site") 2 4 "CATATG" "GTATAC") 
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COMMENT                              "NheI: 1 site") 1 5 "GCTAGC" "CGATCG") 
COMMENT                       #675=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25165752 (8 0) 1 1 1 0 178) 
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COMMENT                              (CGroupPar (CParagraph 25165784 (8 0) 1 1 1 0 178) 
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COMMENT                             "CCNNGG") 
COMMENT                       #677=(CRSFolderPar 
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COMMENT                              (CGroupPar (CParagraph 25165816 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Nli3877I: 9 sites") 5 1 "CYCGRG" 
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COMMENT                                              191) " N2: 3937 " 1))))) 
COMMENT                              "NotI: 2 sites") 2 6 "GCGGCCGC" "CGCCGGCG") 
COMMENT                       #682=(CRSFolderPar 
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COMMENT                                       #685=(CLinePar 
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COMMENT                              "NruI: 2 sites") 3 3 "TCGCGA" "AGCGCT") 
COMMENT                       #686=(CRSFolderPar 
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COMMENT                              (CGroupPar (CParagraph 25166064 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "NsiI: 3 sites") 5 1 "ATGCAT" 
COMMENT                             "TACGTA") 
COMMENT                       #687=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25166096 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "NspI: 7 sites") 5 1 "RCATGY" 
COMMENT                             "YGTACR") 
COMMENT                       #688=(CRSFolderPar 
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COMMENT                              (CGroupPar (CParagraph 25166128 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PacI: 0 sites") 5 3 "TTAATTAA" 
COMMENT                             "AATTAATT") 
COMMENT                       #689=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25166160 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PciI: 3 sites") 1 5 "ACATGT" 
COMMENT                             "TGTACA") 
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COMMENT                                       #693=(CLinePar 
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COMMENT                                              191) " N2: 3776 " 1))))) 
COMMENT                              "PflMI: 2 sites") 7 4 "CCANNNNNTGG" "GGTNNNNNACC") 
COMMENT                       #694=(CRSFolderPar 
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COMMENT                              "PfoI: 1 site") 1 6 "TCCNGGA" "AGGNCCT") 
COMMENT                       #697=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25166256 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PleI: 14 sites") 9 10 
COMMENT                             "GAGTCNNNNNN" "CTCAGNNNNNN") 
COMMENT                       #698=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25166288 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PmeI: 0 sites") 4 4 "GTTTAAAC" 
COMMENT                             "CAAATTTG") 
COMMENT                       #699=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25166320 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PmlI: 5 sites") 3 3 "CACGTG" 
COMMENT                             "GTGCAC") 
COMMENT                       #700=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25166352 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PpiI: 5 sites") 25 20 
COMMENT                             "GAACNNNNNCTCNNNNNNNNNNNNNN" 
COMMENT                             "CTTGNNNNNGAGNNNNNNNNNNNNNN") 
COMMENT                       #701=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25166520 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Ppu10I: 3 sites") 1 5 "ATGCAT" 
COMMENT                             "TACGTA") 
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COMMENT                             (CFolderPar 
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COMMENT                              "PpuMI: 1 site") 2 5 "RGGWCCY" "YCCWGGR") 
COMMENT                       #705=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25166584 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PshAI: 0 sites") 5 5 "GACNNNNGTC" 
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COMMENT                                       #709=(CLinePar 
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COMMENT                                              191) " N2: 5808 " 1))))) 
COMMENT                              "PsiI: 2 sites") 3 3 "TTATAA" "AATATT") 
COMMENT                       #710=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25166648 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Psp03I: 5 sites") 4 1 "GGWCC" 
COMMENT                             "CCWGG") 
COMMENT                       #711=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25166680 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PspGI: 21 sites") 1 6 "NCCWGGN" 
COMMENT                             "NGGWCCN") 
COMMENT                       #712=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25166712 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PspOMI: 5 sites") 1 5 "GGGCCC" 
COMMENT                             "CCCGGG") 
COMMENT                       #713=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25166744 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PsrI: 0 sites") 25 20 
COMMENT                             "GAACNNNNNNTACNNNNNNNNNNNNN" 
COMMENT                             "CTTGNNNNNNATGNNNNNNNNNNNNN") 
COMMENT                       #714=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25166776 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PssI: 9 sites") 5 2 "RGGNCCY" 
COMMENT                             "YCCNGGR") 
COMMENT                       #715=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25166808 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PstI: 5 sites") 5 1 "CTGCAG" 
COMMENT                             "GACGTC") 
COMMENT                       #716=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25166976 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CObjectList 
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COMMENT                                       #719=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #211# 2) 1 2 2 0 
COMMENT                                              191) " N2: 7552 " 1))))) 
COMMENT                              "PvuI: 2 sites") 4 2 "CGATCG" "GCTAGC") 
COMMENT                       #720=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25167008 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PvuII: 4 sites") 3 3 "CAGCTG" 
COMMENT                             "GTCGAC") 
COMMENT                       #721=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25167040 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "RleAI: 6 sites") 18 15 
COMMENT                             "CCCACANNNNNNNNNNNNN" "GGGTGTNNNNNNNNNNNNN") 
COMMENT                       #722=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25167072 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "RsaI: 18 sites") 2 2 "GTAC" 
COMMENT                             "CATG") 
COMMENT                       #723=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25167104 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "RsrII: 0 sites") 2 5 "CGGWCCG" 
COMMENT                             "GCCWGGC") 
COMMENT                       #724=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25167136 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CObjectList 
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COMMENT                                              191) " N1: 1180 " 1) 
COMMENT                                       #727=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #216# 2) 1 2 2 0 
COMMENT                                              191) " N2: 1602 " 1))))) 
COMMENT                              "SacI: 2 sites") 5 1 "GAGCTC" "CTCGAG") 
COMMENT                       #728=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25167168 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "SacII: 3 sites") 4 2 "CCGCGG" 
COMMENT                             "GGCGCC") 
COMMENT                       #729=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25167200 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CParagraph 0 (10 #218# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
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COMMENT                                              191) " N1: 5926 " 1) 
COMMENT                                       #732=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #218# 2) 1 2 2 0 
COMMENT                                              191) " N2: 8103 " 1))))) 
COMMENT                              "SalI: 2 sites") 1 5 "GTCGAC" "CAGCTG") 
COMMENT                       #733=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25167232 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "SanDI: 0 sites") 2 5 "GGGWCCC" 
COMMENT                             "CCCWGGG") 
COMMENT                       #734=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25167264 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #735=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #220# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #736=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #220# 1) 1 2 2 0 
COMMENT                                              191) " N1: 6184 " 1))))) 
COMMENT                              "SapI: 1 site") 8 11 "GCTCTTCNNNNN" "CGAGAAGNNNNN") 
COMMENT                       #737=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25167432 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Sau96I: 35 sites") 1 4 "GGNCC" 
COMMENT                             "CCNGG") 
COMMENT                       #738=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25167464 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #739=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #222# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #740=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #222# 1) 1 2 2 0 
COMMENT                                              191) " N1: 3598 " 1))))) 
COMMENT                              "SbfI: 1 site") 6 2 "CCTGCAGG" "GGACGTCC") 
COMMENT                       #741=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25167496 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #742=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #223# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #743=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #223# 1) 1 2 2 0 
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COMMENT                              "ScaI: 1 site") 3 3 "AGTACT" "TCATGA") 
COMMENT                       #744=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25167528 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #745=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #224# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
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COMMENT                                       #747=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #224# 2) 1 2 2 0 
COMMENT                                              191) " N2: 2557 " 1))))) 
COMMENT                              "SciI: 2 sites") 3 3 "CTCGAG" "GAGCTC") 
COMMENT                       #748=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 25167560 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "ScrFI: 34 sites") 2 3 "CCNGG" 
COMMENT                             "GGNCC") 
COMMENT                       #749=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34309552 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "SelI: 30 sites") 1 5 "NCGCGN" 
COMMENT                             "NGCGCN") 
COMMENT                       #750=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34309584 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                       #753=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #227# 2) 1 2 2 0 
COMMENT                                              191) " N2: 4871 " 1))))) 
COMMENT                              "SexAI: 2 sites") 1 6 "ACCWGGT" "TGGWCCA") 
COMMENT                       #754=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34309616 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "SfaNI: 16 sites") 10 14 
COMMENT                             "GCATCNNNNNNNNNN" "CGTAGNNNNNNNNNN") 
COMMENT                       #755=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34309648 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "SfcI: 12 sites") 1 5 "CTRYAG" 
COMMENT                             "GAYRTC") 
COMMENT                       #756=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34309680 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "SfiI: 0 sites") 8 5 
COMMENT                             "GGCCNNNNNGGCC" "CCGGNNNNNCCGG") 
COMMENT                       #757=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34309848 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #758=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #231# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #759=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #231# 1) 1 2 2 0 
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COMMENT                              "SfoI: 1 site") 3 3 "GGCGCC" "CCGCGG") 
COMMENT                       #760=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34309880 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "SgrAI: 0 sites") 2 6 "CRCCGGYG" 
COMMENT                             "GYGGCCRC") 
COMMENT                       #761=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34309912 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "SimI: 11 sites") 2 5 "GGGTCN" 
COMMENT                             "CCCAGN") 
COMMENT                       #762=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 34309944 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                       #765=(CLinePar 
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FEATURES             Location/Qualifiers
     promoter        386..1545
                     /vntifkey="29"
                     /label=pEF1-alpha
     primer_bind     1546..1565
                     /vntifkey="28"
                     /label=T7
     CDS             2575..2604
                     /vntifkey="4"
                     /label=Myc
     primer_bind     4071..4088
                     /vntifkey="28"
                     /label=BGH\reverse
     polyA_site      4077..4301
                     /vntifkey="26"
                     /label=BGH\polyA
     rep_origin      4347..4775
                     /vntifkey="33"
                     /label=f1\ori
     promoter        4802..5110
                     /vntifkey="29"
                     /label=SV40+ori
     promoter        5165..5231
                     /vntifkey="29"
                     /label=EM-7
     CDS             5232..5630
                     /vntifkey="4"
                     /label=Blasticidin\resistance
     polyA_site      5788..5918
                     /vntifkey="26"
                     /label=SV40\polyA
     rep_origin      complement(6301..6962)
                     /vntifkey="33"
                     /label=pUC\ori
     promoter        complement(8003..8087)
                     /vntifkey="30"
                     /label=bla
     CDS             complement(7108..7968)
                     /vntifkey="4"
                     /label=Ampicilin\resistance
     CDS             1654..1680
                     /vntifkey="4"
                     /label=Flag\tag
     CDS             1687..2400
                     /vntifkey="4"
                     /label=GFP-S65T
     CDS             2437..2523
                     /vntifkey="4"
                     /label=M2-derived\quenching\peptide
     mRNA            2669..3256
                     /vntifkey="54"
                     /label=IRES2
     CDS             3257..3931
                     /vntifkey="4"
                     /label=DsRed-Express2
     CDS             2401..2415
                     /vntifkey="4"
                     /label=DEVD/G
     CDS             2422..2436
                     /vntifkey="4"
                     /label=DEVD/G
BASE COUNT     1911 a      2073 c      2119 g      2001 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg 
     1621 tggtggaatt ctgcagataa acttaagctt accatggatt acaaggacga tgacgataag 
     1681 gtacctatga gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta 
     1741 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca 
     1801 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca 
     1861 acacttgtca ctactttcac ttatggtgtt caatgctttt caagataccc agatcatatg 
     1921 aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata 
     1981 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc 
     2041 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga 
     2101 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag 
     2161 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta 
     2221 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac 
     2281 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg 
     2341 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa 
     2401 gatgaagtag atggggctag cgatgaagta gatgggtgca acgattcaag tgaccctctt 
     2461 gttgttgccg cgagtatcat tgggatcttg cacttgatat tgtggattct tgatcgtctt 
     2521 tacgggcccg tttatccagc acagtggcgg ccgctcgagt ctagagggcc cttcgaacaa 
     2581 aaactcatct cagaagagga tctgaatatg cataccggtc atcatcacca tcaccattga 
     2641 gtttgacggt accgcgggcc cgggatccgc ccctctccct cccccccccc taacgttact 
     2701 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata 
     2761 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt 
     2821 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa 
     2881 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag 
     2941 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca 
     3001 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc 
     3061 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat 
     3121 tgtatgggat ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta 
     3181 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga 
     3241 taatatggcc acaaccatgg atagcactga gaacgtcatc aagcccttca tgcgcttcaa 
     3301 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg 
     3361 caagccctac gagggcaccc agaccgccaa gctgcaggtg accaagggcg gccccctgcc 
     3421 cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt acgtgaagca 
     3481 ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg 
     3541 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga 
     3601 cggcaccttc atctaccacg tgaagttcat cggcgtgaac ttcccctccg acggccccgt 
     3661 aatgcagaag aagactctgg gctgggagcc ctccaccgag cgcctgtacc cccgcgacgg 
     3721 cgtgctgaag ggcgagatcc acaaggcgct gaagctgaag ggcggcggcc actacctggt 
     3781 ggagttcaag tcaatctaca tggccaagaa gcccgtgaag ctgcccggct actactacgt 
     3841 ggactccaag ctggacatca cctcccacaa cgaggactac accgtggtgg agcagtacga 
     3901 gcgcgccgag gcccgccacc acctgttcca gtaggcggcc gcgactctag atcataatca 
     3961 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 
     4021 acctgaaaca taaaatgaat gcaattgttg ttgttaaacc cgctgatcag cctcgactgt 
     4081 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga 
     4141 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag 
     4201 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga 
     4261 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac 
     4321 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg 
     4381 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 
     4441 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 
     4501 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 
     4561 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 
     4621 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 
     4681 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 
     4741 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta 
     4801 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 
     4861 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 
     4921 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 
     4981 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 
     5041 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga 
     5101 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag 
     5161 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga 
     5221 ggaactaaac catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa 
     5281 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct 
     5341 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg 
     5401 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg 
     5461 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc 
     5521 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg 
     5581 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcacttcgtg 
     5641 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa 
     5701 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 
     5761 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 
     5821 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 
     5881 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 
     5941 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 
     6001 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 
     6061 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 
     6121 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 
     6181 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 
     6241 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 
     6301 atgtgagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 
     6361 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 
     6421 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 
     6481 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 
     6541 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 
     6601 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 
     6661 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 
     6721 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 
     6781 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 
     6841 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 
     6901 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 
     6961 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 
     7021 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 
     7081 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 
     7141 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 
     7201 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 
     7261 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 
     7321 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 
     7381 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 
     7441 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 
     7501 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 
     7561 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 
     7621 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 
     7681 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 
     7741 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 
     7801 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 
     7861 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 
     7921 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 
     7981 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 
     8041 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 
     8101 cgtc 
//
