LOCUS       peNBG1fl-STOPmut        5973 bp    DNA     circular     23-SEP-2005
DEFINITION  LMBP 7667.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|peNBG1fl STOPmut|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     primer_bind     151..171
                     /vntifkey="28"
                     /label=FVR3256
     primer_bind     2086..2105
                     /vntifkey="28"
                     /label=FVR3592
     misc_feature    2251..2253
                     /vntifkey="21"
                     /label=Asn
     CDS             2066..2093
                     /vntifkey="4"
                     /label=T2
     misc_feature    1891..1934
                     /vntifkey="21"
                     /label=T1
     CDS             1150..1723
                     /vntifkey="4"
                     /label=pUC\ori
     CDS             236..1045
                     /vntifkey="4"
                     /label=Kan
     misc_feature    14..113
                     /vntifkey="21"
                     /label=attL2
     misc_feature    2143..2242
                     /vntifkey="21"
                     /label=attL1
BASE COUNT     1714 a      1380 c      1524 g      1355 t 
ORIGIN
        1 tcgagatatc tagacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta 
       61 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca 
      121 gctgcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat 
      181 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag 
      241 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata 
      301 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta 
      361 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga 
      421 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat 
      481 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa 
      541 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct 
      601 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga 
      661 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag 
      721 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa 
      781 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct 
      841 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga 
      901 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca 
      961 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca 
     1021 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac attattcaga 
     1081 ttgggccccg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 
     1141 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 
     1201 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 
     1261 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 
     1321 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 
     1381 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 
     1441 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 
     1501 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 
     1561 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 
     1621 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 
     1681 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 
     1741 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 
     1801 ccctgattct gtggataacc gtattaccgc tagcatggat ctcggggacg tctaactact 
     1861 aagcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc ggaagactgg 
     1921 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg 
     1981 ggagcggatt tgaacgttgt gaagcaacgg cccggagggt ggcgggcagg acgcccgcca 
     2041 taaactgcca ggcatcaaac taagcagaag gccatcctga cggatggcct ttttgcgttt 
     2101 ctacaaactc ttcctgttag ttagttactt aagctcgggc cccaaataat gattttattt 
     2161 tgactgatag tgacctgttc gttgcaacaa attgataagc aatgcttttt tataatgcca 
     2221 actttgtaca aaaaagcagg ctggcgccgg aaccaattca gtcgactgga tcacattttt 
     2281 cacaacatcc ctggctccac ctcttctgcc acaaacgtca gcatggtggt atcagccggc 
     2341 ccttggtcca gcgagaaggc agagacgaac attttagaaa tcaacgagaa attgcgcccc 
     2401 cagctggcag agaacaaaca gcagttcaga aacctcaaag agaaatgttt tgtaactcaa 
     2461 ctggccggct tcctggccaa ccgacagaag aaatacaagt atgaagagtg taaagacctc 
     2521 ataaaattta tgctgaggaa tgagcgacag ttcaaggagg agaagcttgc agagcagctc 
     2581 aagcaagctg aggagctcag gcaatataaa gtcctggttc actctcagga acgggagctg 
     2641 acccagttaa gggagaagtt acgggaaggg agagatgcct cccgctcatt gaatcagcat 
     2701 ctccaggccc tcctcactcc agatgagcca gacaagtccc aggggcagga cctccaagaa 
     2761 cagctggctg aggggtgtag actggcacag caccttgtcc aaaagctcag cccagaaaat 
     2821 gacgaagatg aggatgaaga tgttcaagtt gaggaggctg agaaagtact ggaatcatct 
     2881 gcccccaggg aggtgcagaa ggctgaagaa agcaaagtcc ctgaggactc actggaggaa 
     2941 tgtgccatca cttgttcaaa tagccacggc ccttgtgact ccaaccagcc tcacaagaac 
     3001 atcaacatca catttgagga agacaaagtc aactcaactc tggttgtaga cagagaatcc 
     3061 tctcatgatg aatgtcagga tgctgtaaac attctcccag tccctggccc cacctcttct 
     3121 gccacaaacg tcagcatggt ggtatcagcc ggccctttgt ccagcgagaa ggcagagatg 
     3181 aacattctag aaatgaatga gaaattgcgc ccccagctgg cagagaagaa acagcagttc 
     3241 agaaacctca aagagaaatg ttttgtaact caactggccg gcttcctggc caaccagcag 
     3301 aacaaataca aatatgaaga gtgcgaagac ctcataaaat ctatgctgag gaatgagcga 
     3361 cagttcaagg aggagaagct tgcagagcag ctcaagcaag ctgaggagct caggcaatat 
     3421 aaagtcctgg ttcactctca ggaacgagag ctgacccagt taagggagaa gttacgggaa 
     3481 gggagagatg cctcccgctc attgaatcag catctccagg ccctcctcac tccggatgag 
     3541 ccagacaagt cccaggggca ggacctccaa gaacagctgg ctgaggggtg tagactggca 
     3601 cagcaccttg tccaaaagct cagcccagaa aatgacgaag atgaggatga agatgttcaa 
     3661 gttgaggagg ctgagaaagt actggaatca tctgccccca gggaggtgca gaaggctgaa 
     3721 gaaagcaaag tccctgagga ctcactggag gaatgtgcca tcacttgttc aaatagccac 
     3781 ggcccttgtg actccaacca gcctcacaag aacatcaaca tcacatttga ggaagacaaa 
     3841 gtcaactcag ctctggttgt agacagagaa tcctctcatg atgaatgtca ggatgctgta 
     3901 aacattctcc cagtccctgg ccccacctct tctgccacaa acgtcagcat ggtggtatca 
     3961 gccggccctt tgtccagcga gaaggcagag atgaacattc tagaaatgaa tgagaaattg 
     4021 cgcccccagc tggcagagaa gaaacagcag ttcagaaacc tcaaagagaa atgttttgta 
     4081 actcaactgg cctgcttcct ggccaaccag cagaacaaat acaaatatga agagtgcaaa 
     4141 gacctcataa aatctgtgct gaggaatgag cgacagttca aggaggagaa gcttgcagag 
     4201 cagctcaagc aagctgagga gctcaggcaa tataaagtcc tggttcactc tcaggaacgg 
     4261 gagctgaccc agttaaggga gaagttacgg gaagggagag atgcctcccg ctcattgaat 
     4321 cagcatctcc aggccctcct cactccggat gagccagaca agtcccaggg gcaggacctc 
     4381 caagaacagc tggctgaggg gtgtagactg gcacagcaac ttttccaaaa gctcagccca 
     4441 gaaaatgaca atgatcacga tgaagatgtt caagttgagg tggctgagaa agtgcagaaa 
     4501 tcgtctgccc ccagggagat gcagaaggct gaagaaaagg aagtccctga ggactcactg 
     4561 gaggaatgtg ccatcacttg ttcaaatagc catggccctt atgactccaa ccagccacat 
     4621 aggaaaacca aaatcacatt tgaggaagac aaagtcgact caactctcat tggctcatcc 
     4681 tctcatgttg aatgggagga tgctgtacac attatcccag aaaatgaaag tgatgatgag 
     4741 gaagaggaag aaaaagggcc agtgtctccc aggaatctgc aggagtctga agaggaggaa 
     4801 gtcccccagg agtcctggga tgaaggttat tcgactctct caattcctcc tgaaatgttg 
     4861 gcctcgtacc agtcttacag cggcacattt cactcattag aggaacagca agtctgcatg 
     4921 gctgttgaca taggcggaca tcggtgggat caagtgaaaa aggaggacca agaggcaaca 
     4981 ggtcccaggc tcagcaggga gctgctggat gagaaagggc ctgaagtctt gcaggactca 
     5041 ctggatagat gttattcaac tccttcaggt tatcttgaac tgaccgactc atgccagccc 
     5101 tacagaagtg ccttttacat attggagcaa cagcgtgttg gctgggctct tgacatggat 
     5161 gaaattgaaa agtaccaaga agtggaagaa gaccaagacc catcatgccc caggctcagc 
     5221 agggagctgc tggatgagaa agagcctgaa gtcttgcagg actccctgga tagatgttat 
     5281 tcgactcctt caggttatct tgaactgcct gacttaggcc agccctacag aagtgctgtt 
     5341 tactcattgg aggaacagta ccttggcttg gctcttgacg tggacagaat taaaaaggac 
     5401 caggaagagg aagaagacca aggcccacca tgccccaggc tcagcaggga gctgctggag 
     5461 gcagtagagc ctgaagtctt gcaggactca ctggatagat gttattcaac tccttccagt 
     5521 tgtcttgaac agcctgactc ctgcctgccc tatggaagtt ccttttatgc attggaggaa 
     5581 aaacatgttg gcttttctct tgacgtggga gaaattgaaa agaaggggaa ggggaagaaa 
     5641 agaaggggaa gaagatcaac gaagaaaaga aggagaaggg gaagaaaaga aggggaagaa 
     5701 gatcaaaacc caccatgccc caggctcagc ggcatgctga tggaagtgga agagcctgaa 
     5761 gtcttgcagg actcactgga tagatgttat tcgactccgt caatgtactt tgaactacct 
     5821 gactcattcc agcactacag aagtgtgttt tactcatttg aggaacagca catcagcttc 
     5881 gcccttgacg tggacaatag gtttcttact ttgatgggaa caagtctcca cctggtcttc 
     5941 cagatgggag tcatattccc acagttagca gcc 
//
