LOCUS       pEF6-HA-TAB3            7976 bp    DNA     circular     19-APR-2012
DEFINITION  LMBP 7789.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|623787657|
COMMENT     VNTNAME|pEF6-HA-TAB3|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
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COMMENT             (CObList) (CTerm 1 0 0 "EcoRV" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "EcoRI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 1701707897 0 "" 0 "" "" "" "" "" "" "pCMV5-HA-TAB3" 
COMMENT             "" 0 0 1 2190 5787 7976 921 3110 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "EcoRI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "SmaI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
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COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
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COMMENT            4845 0 5360 0 5477 0 7165 0 7861 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 5764 0 7971 0 0 "") 
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COMMENT        #7=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 4426 0 5746 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 1067 0 1287 0 7887 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 4828 0 5333 0 7265 0 7817 
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COMMENT             (CStringList) 1 1 1 1 "") 
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COMMENT             (CStringList) 1 1 1 1 "") 
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COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "EM-7" 29 0 0 1221 1287 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "SV40+ori" 29 0 0 858 1166 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "f1 ori" 33 0 0 403 831 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #20=(CFSignal (CObList) "BGH polyA" 26 0 0 133 357 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "BGH reverse" 28 0 0 127 144 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #22=(CFSignal (CObList) "His6" 21 0 0 91 144 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #23=(CFSignal (CObList) "Myc" 4 0 0 42 71 1 (CStringList) (CStringList) 
COMMENT             1 1 1 1 "") 
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COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #25=(CFSignal (CObList) "pEF1-alpha" 29 0 0 4546 5705 1 (CStringList) 
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COMMENT        #26=(CFSignal (CObList) "stop" 4 0 0 7967 7969 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #27=(CFSignal (CObList) "start" 4 0 0 5798 5800 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #28=(CFSignal (CObList) "HA" 4 0 0 5801 5827 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #29=(CFSignal (CObList) "TAB3" 4 0 0 5834 7966 1 (CStringList) 
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COMMENT                          "Local object" 1) 
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COMMENT                          "Created: 04/19/12 06:20" 1) 
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COMMENT                          "Last Modified: 04/19/12 06:20" 1) 
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COMMENT                          "length: 7976 bp" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                   "Annotations") 
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COMMENT                                 "original length: 5786" 1) 
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COMMENT                          "#1: FRAGMENT of <a href=\"vnti:dnarna/pEF6%2Fmyc-his%20A?sel=1639-1626\">pEF6/myc-his A</a> ") 
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COMMENT                          "CDS (7 total)") 
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COMMENT                                 "BGH reverse") 
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COMMENT                          "Primer Binding Site (2 total)") 
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COMMENT                                 "pEF1-alpha"))) "Promoter Eukaryotic (3 total)") 
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FEATURES             Location/Qualifiers
     CDS             complement(3164..4024)
                     /vntifkey="4"
                     /label=Ampicilin\resistance
     promoter        complement(4059..4143)
                     /vntifkey="30"
                     /label=bla
     rep_origin      complement(2357..3018)
                     /vntifkey="33"
                     /label=pUC\ori
     polyA_site      1844..1974
                     /vntifkey="26"
                     /label=SV40\polyA
     CDS             1288..1686
                     /vntifkey="4"
                     /label=Blasticidin\resistance
     promoter        1221..1287
                     /vntifkey="29"
                     /label=EM-7
     promoter        858..1166
                     /vntifkey="29"
                     /label=SV40+ori
     rep_origin      403..831
                     /vntifkey="33"
                     /label=f1\ori
     polyA_site      133..357
                     /vntifkey="26"
                     /label=BGH\polyA
     primer_bind     127..144
                     /vntifkey="28"
                     /label=BGH\reverse
     misc_feature    91..144
                     /vntifkey="21"
                     /label=His6
     CDS             42..71
                     /vntifkey="4"
                     /label=Myc
     primer_bind     5706..5725
                     /vntifkey="28"
                     /label=T7
     promoter        4546..5705
                     /vntifkey="29"
                     /label=pEF1-alpha
     CDS             7967..7969
                     /vntifkey="4"
                     /label=stop
     CDS             5798..5800
                     /vntifkey="4"
                     /label=start
     CDS             5801..5827
                     /vntifkey="4"
                     /label=HA
     CDS             5834..7966
                     /vntifkey="4"
                     /label=TAB3
BASE COUNT     1961 a      2080 c      1954 g      1981 t 
ORIGIN
        1 atccagcaca gtggcggccg ctcgagtcta gagggccctt cgaacaaaaa ctcatctcag 
       61 aagaggatct gaatatgcat accggtcatc atcaccatca ccattgagtt taaacccgct 
      121 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 
      181 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 
      241 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 
      301 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt 
      361 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg 
      421 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 
      481 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 
      541 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 
      601 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 
      661 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 
      721 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 
      781 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg 
      841 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 
      901 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 
      961 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 
     1021 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 
     1081 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 
     1141 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 
     1201 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta 
     1261 taatacgaca aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga agaatccacc 
     1321 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc 
     1381 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt 
     1441 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc 
     1501 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga 
     1561 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt 
     1621 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag 
     1681 ggctaagcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac 
     1741 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 
     1801 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc 
     1861 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 
     1921 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc 
     1981 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 
     2041 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 
     2101 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 
     2161 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 
     2221 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 
     2281 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
     2341 taacgcagga aagaacatgt gagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 
     2401 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 
     2461 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 
     2521 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 
     2581 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 
     2641 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
     2701 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
     2761 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
     2821 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 
     2881 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 
     2941 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 
     3001 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 
     3061 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 
     3121 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 
     3181 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 
     3241 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 
     3301 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 
     3361 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 
     3421 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 
     3481 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 
     3541 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 
     3601 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 
     3661 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 
     3721 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 
     3781 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 
     3841 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 
     3901 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 
     3961 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 
     4021 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 
     4081 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 
     4141 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct 
     4201 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt 
     4261 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga 
     4321 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc 
     4381 cagatatacg cgttgacatt gattattgac taggcttttg caaaaagctt tgcaaagatg 
     4441 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg 
     4501 agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga 
     4561 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa 
     4621 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta 
     4681 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca 
     4741 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt 
     4801 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg 
     4861 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt 
     4921 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg 
     4981 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct 
     5041 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt 
     5101 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg 
     5161 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc 
     5221 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg 
     5281 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc 
     5341 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag 
     5401 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag 
     5461 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt 
     5521 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca 
     5581 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa 
     5641 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt 
     5701 ggtactaata cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc 
     5761 tcggatccac tagtccagtg tggtggaatt cgccaccatg tacgcatacg atgttccaga 
     5821 ttacgcggta ccggcgcaaa gcagcccaca gcttgatatt caggttctcc atgatcttcg 
     5881 acaacgtttc cctgaaattc cagagggcgt ggtgtctcag tgcatgttac agaataacaa 
     5941 caatcttgaa gcctgttgcc gagccctttc ccaggagagt agcaaatact tatatatgga 
     6001 ataccatagt ccagatgaca ataggatgaa tagaaatcgc cttttacata ttaacctggg 
     6061 tatccattct cctagtagct atcacccagg agatggagcc caacttaatg gtggtcgaac 
     6121 actggtacat agctcaagtg atggacatat tgatcctcag catgcagcag gtaaacagct 
     6181 gatatgttta gttcaagaac cacactcagc tccagctgtt gttgctgcta ctcccaacta 
     6241 caatccattt tttatgaacg aacagaacag aagtgcagct actcctcctt cacaaccacc 
     6301 tcaacagcca tcttccatgc aaacaggaat gaatccgtct gctatgcaag ggccttcacc 
     6361 accaccgcca cctccttcat acatgcacat acctcggtat agtacaaatc caattactgt 
     6421 tacagtatcc cagaacctcc cttctggaca gactgtacca agagctttac aaattcttcc 
     6481 acaaattcca agcaatctct atgggtctcc tggttctatt tatattagac agacatctca 
     6541 gagttcatca ggaagacaaa ctcctcagag tacgccgtgg cagtcctcac cacagggccc 
     6601 agtgcctcac tatagccagc gtcctttacc tgtttatcca caccaacaga actatcagcc 
     6661 ttctcagtat tctcccaaac agcagcagat ccctcagtct gcttaccatt caccacctcc 
     6721 ttctcaatgt ccttcaccct tcagctctcc acagcatcaa gtgcaacctt cccagttggg 
     6781 ccacatcttt atgccaccta gtccttcaac tactccaccc catccatatc aacaaggacc 
     6841 tcctagctat cagaaacagg gaagccattc agtagcctat cttccataca cagcatctag 
     6901 tttatccaaa ggttccatga agaagataga aattacagtt gaaccttctc aaagacctgg 
     6961 gacagcaatt aataggagtc cttcacccat cagtaatcaa ccatctccac ggaatcaaca 
     7021 ctcactgtac acagccacca cgccaccttc aagttctcct tcaagaggga tatctagtca 
     7081 accaaaacct ccatttagtg ttaatcctgt gtatattaca tatacacagc caactggacc 
     7141 ttcttgtact ccatcaccat ctcctcgagt gataccaaac ccaactacag tttttaaaat 
     7201 taccgtaggc cgagcaacga ctgaaaatct tttaaattta gtggaccaag aagagcgctc 
     7261 tgcagcacca gaacctattc agcccatttc agtgatacca ggctctgggg gagaaaaggg 
     7321 aagccataaa tatcagagaa gttctagttc tggatcagat gactatgcct acacacaagc 
     7381 cttgctgtta catcaacgag caaggatgga gaggttagca aagcaactga aacttgagaa 
     7441 agaggagcta gagcggttga agtctgaagt taatggtatg gagcatgacc tgatgcagag 
     7501 acggctcaga agagtcagct gcaccactgc gatccctacg cctgaggaaa tgacaagatt 
     7561 gagaagcatg aacagacaac tccagataaa tgttgactgt acactgaaag aagttgacct 
     7621 ccttcaatct agaggaaact ttgatccaaa agccatgaat aatttttatg acaacataga 
     7681 acctggccca gttgtaccac ccaagccatc taaaaaagac tcctcagacc cctgcacaat 
     7741 tgagagaaaa gcccgaagaa ttagcgtgac ctccaaagta caggcagaca tccatgacac 
     7801 ccaggcagca gctgcagatg agcatcgaac tggctccaca caaagtcctc ggacacaacc 
     7861 tcgagatgaa gactacgaag gggctccatg gaattgtgat agctgcacct ttcttaacca 
     7921 cccagcacta aatcgctgtg agcagtgcga gatgccacgg tacacctgag gatccc 
//
