LOCUS       pENTR3C-mRIP4        4639 bp    DNA     circular     27-JUL-2007
DEFINITION  LMBP 7900.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|410094914|
COMMENT     VNTNAME|pENTR3C-mRIP4|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 62 0 576 0 0 "") 
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COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 4639 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 6 746 0 998 0 1704 0 1907 0 
COMMENT            1942 0 2489 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "") 
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COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #225=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -160 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList) 1952670051 
COMMENT       (CStringList) 1667462241 1952671604 (CObList)))
FEATURES             Location/Qualifiers
     terminator      complement(4428..4455)
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      complement(4253..4296)
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     rep_origin      3471..4144
                     /vntifkey="33"
                     /label=pUC\origin
     CDS             2598..3407
                     /vntifkey="4"
                     /label=Kan(R)
     misc_recomb     complement(2376..2475)
                     /vntifkey="86"
                     /label=attL2
     misc_recomb     4505..4604
                     /vntifkey="86"
                     /label=attL1
     CDS             <1..2361
                     /vntifkey="4"
                     /label=mRIP4+STOP
     misc_signal     <4637..4639
                     /vntifkey="87"
                     /label=Kozak
                     /note="Kozak sequence"
     exon            183..479
                     /vntifkey="61"
                     /label=exon\2
     misc_feature    <937..1201
                     /vntifkey="21"
                     /label=exon7
BASE COUNT     1124 a      1169 c      1250 g      1096 t 
ORIGIN
        1 atggagggcg agggccgggg ccggtgggct ctggggctgc tgcgcacctt cgacgccggc 
       61 gaattcgcag gctgggagaa ggtgggctcg ggcggcttcg ggcaggtgta caaggtgcgc 
      121 catgtgcact ggaagacgtg gctcgcgatc aagtgctcgc ccagtctgca cgtcgacgac 
      181 agggaacgaa tggagctcct ggaggaagct aagaagatgg agatggccaa gttccgatac 
      241 attctacctg tgtacggcat atgccaggaa cctgtcggct tggtcatgga gtacatggag 
      301 acaggctccc tggagaagct gctggcctca gagccattgc cttgggacct gcgctttcgc 
      361 atcgtgcacg agacagccgt gggcatgaac ttcctgcatt gcatgtctcc gccactgctg 
      421 cacctagacc tgaagccagc gaacatcctg ctggatgccc actaccatgt caagatttct 
      481 gactttgggc tggccaagtg caatggcatg tcccactctc atgacctcag catggatggc 
      541 ctgtttggta caatcgctta cctccctcca gagcgaattc gtgagaagag ccgcttgttt 
      601 gacaccaaac atgatgtata cagcttcgcc attgtgatct ggggtgtgct tacacagaag 
      661 aagccatttg cagatgaaaa gaacatccta cacatcatga tgaaagtggt aaagggccac 
      721 cgcccagagc tgccacccat ctgcagaccc cggccgcgtg cctgtgccag cctgataggg 
      781 ctcatgcaac ggtgctggca tgcagaccca caggtgcggc ccaccttcca agaaattacc 
      841 tctgaaacag aagacctttg tgagaagcct gatgaggagg tgaaagacct ggctcatgag 
      901 ccaggcgaga aaagctctct agagtccaag agtgaggcca ggcccgagtc ctcacgcctc 
      961 aagcgcgcct ctgctccccc cttcgataac gactgcagtc tctccgagtt gctgtcacag 
     1021 ttggactctg ggatctccca gactcttgaa ggccccgaag agctcagccg aagttcctct 
     1081 gaatgcaagc tcccatcgtc cagcagtggc aagaggctct cgggggtgtc ctcagtggac 
     1141 tcagcctttt cctccagagg atcgctgtca ctgtcttttg agcgggaagc ttcaacaggc 
     1201 gacctgggcc ccacagacat ccagaagaag aagctagtgg atgccatcat atcaggggac 
     1261 accagcaggc tgatgaagat cctacagccc caagatgtgg acttggttct agacagcagt 
     1321 gccagcctgc tgcacctggc tgtggaggcc ggacaggagg agtgtgtcaa gtggctgctg 
     1381 cttaacaatg ccaaccccaa cctgaccaac aggaagggct ctacaccact gcatatggct 
     1441 gtggagcgga agggacgtgg aattgtggag ctactgctag cccggaagac cagtgtcaat 
     1501 gccaaggatg aagaccagtg gactgccctg cactttgcag cccagaatgg ggatgaggcc 
     1561 agcacaaggc tgctgctaga gaagaatgct tctgtcaatg aggtggactt tgagggccga 
     1621 acacccatgc atgtagcctg ccagcatgga caggagaaca ttgtgcgcac cctgctccgc 
     1681 cgtggtgtgg atgtgggcct gcagggaaag gatgcctggt tgcctctgca ctatgctgcc 
     1741 tggcagggcc accttcccat tgttaagctg ctagccaagc agcctggggt gagtgtgaat 
     1801 gcccagacac tagacgggag gacacccctg cacctggctg ctcagagggg gcattaccgt 
     1861 gtggctcgca ttctcattga cctgtgctct gatgttaaca tctgcagcct acaggcacag 
     1921 acacctctgc atgttgctgc agagactgga cacactagta ctgccaggct actcttgcat 
     1981 cgtggtgctg gcaaggaggc tttgacctca gagggctata ctgccttgca cctggcagcc 
     2041 cagaatggac acctggctac tgtcaagctg ctcatagagg agaaggctga tgtgatggct 
     2101 cggggtcccc tgaatcagac agcactgcac ctggctgctg cccgtggaca ctcagaggtg 
     2161 gtagaagagc tggtcagtgc tgacctcatt gacctgtctg atgagcaggg cctcagcgca 
     2221 ctgcacctgg ctgctcaggg caggcattca cagactgtgg agacactgct caaacatgga 
     2281 gcacacatca acttgcagag tctcaagttc caaggaggcc agagctctgc tgccacgttg 
     2341 ctccgacgca gcaagaccta gctcgagata tctagaccca gctttcttgt acaaagttgg 
     2401 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa 
     2461 taaaatcatt atttgccatc cagctgcagc tctggcccgt gtctcaaaat ctctgatgtt 
     2521 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca 
     2581 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt 
     2641 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag 
     2701 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg 
     2761 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg 
     2821 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac 
     2881 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag 
     2941 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt 
     3001 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga 
     3061 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac 
     3121 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg 
     3181 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg 
     3241 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg 
     3301 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg 
     3361 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta 
     3421 attggttgta acattattca gattgggccc cgttccactg agcgtcagac cccgtagaaa 
     3481 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 
     3541 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 
     3601 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 
     3661 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 
     3721 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 
     3781 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 
     3841 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 
     3901 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 
     3961 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 
     4021 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 
     4081 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 
     4141 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gctagcatgg 
     4201 atctcgggga cgtctaacta ctaagcgaga gtagggaact gccaggcatc aaataaaacg 
     4261 aaaggctcag tcggaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 
     4321 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gtgaagcaac ggcccggagg 
     4381 gtggcgggca ggacgcccgc cataaactgc caggcatcaa actaagcaga aggccatcct 
     4441 gacggatggc ctttttgcgt ttctacaaac tcttcctgtt agttagttac ttaagctcgg 
     4501 gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa 
     4561 gcaatgcttt tttataatgc caactttgta caaaaaagca ggctctttaa aggaaccaat 
     4621 tcagtcgact ggatccacc 
//
