LOCUS pDG1i-eGFP-V5\bl 9097 bp DNA circular 23-JUN-2023 DEFINITION LMBP 7941. SOURCE ORGANISM COMMENT Accession number: LMBP 7941 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) Last modified: 23-JUN-2023 NGS data implemented FEATURES Location/Qualifiers misc_feature 2506..2547 /label=V5\epitope promoter 2966..3031 /label=synthetic\EM7\promoter CDS 268..927 /label=chloramphenicol\resistance CDS 1269..1574 /label=ccdB CDS 3032..3430 /label=blasticidin\resistance misc_recomb 1615..1739 /label=attR2 misc_recomb complement(35..159) /label=attR1 CDS 1771..2487 /label=EGFP promoter 2580..2861 /label=SV40\early\promoter rep_origin 2818..2893 /label=SV40\ori terminator 3822..3896 /label=SV40\polyA\early promoter 7078..7406 /label=RSV/HIV-1\5'\LTR rep_origin 4325..4780 /label=f1\ori CDS 4911..5771 /label=ampicillin\resistance rep_origin 5942..6533 /label=pMB1\ori promoter 6806..6889 /label=lac\promoter promoter 6953..6969 /label=T3\promoter terminator complement(3822..3896) /label=SV40\polyA\late rep_origin 4032..4107 /label=SV40\ori promoter complement(4141..4157) /label=T7\promoter misc_feature 7517..7561 /label=HIV-1\psi\packaging\signal misc_feature 8071..8304 /label=HIV-1\Rev\response\element terminator 3516..3750 /label=RSV/HIV-1\dU3/3'\LTR misc_feature 3500..3515 /label=HIV-1\cPPT promoter 8821..9089 /label=hCMVTRE\promoter promoter 167..219 /label=lacUV5\promoter BASE COUNT 2431 a 2135 c 2292 g 2239 t ORIGIN 1 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg 61 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac 121 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca 181 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg 241 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt 301 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt 361 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat 421 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg 481 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt 541 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac 601 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg 661 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg 721 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc 781 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt 841 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac 901 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa 961 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc 1021 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg 1081 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac 1141 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg 1201 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg 1261 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg 1321 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca 1381 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg 1441 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg 1501 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt 1561 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt 1621 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt 1681 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt 1741 gatatccagc acagtggcgg ccgctcgagt atggtgagca agggcgagga gctgttcacc 1801 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 1861 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 1921 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag 1981 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 2041 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 2101 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 2161 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac 2221 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac 2281 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 2341 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 2401 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 2461 actctcggca tggacgagct gtacaaggag ggcccgcggt tcgaaggtaa gcctatccct 2521 aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagtttg gaattaattc 2581 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 2641 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 2701 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 2761 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 2821 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 2881 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 2941 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat 3001 agtataatac gacaaggtga ggaactaaac catggccaag cctttgtctc aagaagaatc 3061 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag 3121 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca 3181 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc 3241 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg 3301 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga 3361 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg 3421 ggagggctaa gcacaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg 3481 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 3541 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 3601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 3661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 3721 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 3781 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt 3841 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 3901 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc 3961 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 4021 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 4081 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc 4141 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa 4201 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 4261 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 4321 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 4381 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 4441 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 4501 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 4561 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 4621 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 4681 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 4741 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa 4801 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 4861 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 4921 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 4981 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 5041 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 5101 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 5161 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 5221 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 5281 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 5341 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 5401 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 5461 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 5521 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 5581 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 5641 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 5701 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 5761 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 5821 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 5881 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 5941 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 6001 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 6061 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 6121 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 6181 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 6241 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 6301 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 6361 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 6421 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 6481 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 6541 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 6601 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 6661 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 6721 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 6781 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt 6841 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg 6901 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc 6961 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt 7021 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca 7081 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca 7141 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt 7201 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga 7261 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 7321 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 7381 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg 7441 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc 7501 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga 7561 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt 7621 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg 7681 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa 7741 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat 7801 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa 7861 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc 7921 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa 7981 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt 8041 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc 8101 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt 8161 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct 8221 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag 8281 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac 8341 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca 8401 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt 8461 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa 8521 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt 8581 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt 8641 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag 8701 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc 8761 cattcgatta gtgaacggat ctcgacggta tcgatggtcc gaggttctag acgagtttac 8821 tccctatcag tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat 8881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga 8941 tagagaacgt atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc 9001 cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag 9061 cagagctcgt ttagtgaacc gtcagatcgc accggtc //