ID pDG2i-LUC-Flag-puro standard; circular DNA; UNA; 9883 BP. DE LMBP 7949. CC Accession number: LMBP 7949 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 28-JUN-2017 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid CC VNTREPLTYPE|Phage CC VNTREPLTYPE|Virus CC VNTEXTCHREPL|Bacteria CC VNTEXTCHREPL|Animal/Other Eukaryotic FH Key Location/Qualifiers FH FT Misc._feature complement(join(9876..9883,1..13)) FT /vntifkey="21" FT /label=attB2 FT /note="Unknown feature type:Misc._feature" FT CDS 8201..9856 FT /vntifkey="4" FT /label=LUC FT CDS 508..1215 FT /vntifkey="4" FT /label=rtTA3 FT RBS 1272..1844 FT /vntifkey="32" FT /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES) FT CDS 1847..2449 FT /vntifkey="4" FT /label=puromycin\resistance FT terminator 2846..2920 FT /vntifkey="43" FT /label=SV40\polyA\early FT promoter 6102..6430 FT /vntifkey="29" FT /label=RSV/HIV-1\5'\LTR FT rep_origin 3349..3804 FT /vntifkey="33" FT /label=f1\origin FT CDS 3935..4795 FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 5336..5738 FT /vntifkey="33" FT /label=pMB1\ori FT promoter 5830..5913 FT /vntifkey="30" FT /label=lac\promoter FT promoter 5977..5993 FT /vntifkey="30" FT /label=T3\promoter FT terminator complement(2846..2920) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin 3056..3131 FT /vntifkey="33" FT /label=SV40\ori FT promoter complement(3165..3181) FT /vntifkey="30" FT /label=T7\promoter FT misc_feature 6541..6585 FT /vntifkey="21" FT /label=HIV-1\psi\packaging\signal FT misc_feature 7095..7328 FT /vntifkey="21" FT /label=HIV-1\Rev\response\element FT terminator 2540..2774 FT /vntifkey="43" FT /label=RSV/HIV-1\dU3/3'\LTR FT misc_feature 2524..2539 FT /vntifkey="21" FT /label=HIV-1\cPPT FT promoter 7846..8113 FT /vntifkey="29" FT /label=hCMVTRE\promoter FT promoter 117..399 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 356..431 FT /vntifkey="33" FT /label=SV40\ori FT misc_feature 1216..1249 FT /vntifkey="21" FT /label=loxP FT misc_marker 58..81 FT /vntifkey="22" FT /label=FLAG\epitope FT attenuator 8156..8180 FT /vntifkey="0" FT /label=attB1 FT attenuator complement(9873..14) FT /vntifkey="0" FT /label=attB2 SQ Sequence 9883 BP; 2499 A; 2559 C; 2595 G; 2230 t; ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccggac 60 tacaaggatg acgatgacaa aacgcgtacc ggttagtaat gagtttggaa ttaattctgt 120 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg 180 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 240 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 300 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 360 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag 420 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc 480 attttcggat ctgatcagca cgtgaccatg tctaggctgg acaagagcaa agtcataaac 540 ggagctctgg aattactcaa tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct 600 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg 660 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa 720 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc 780 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa 840 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg 900 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat 960 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga 1020 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa 1080 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc 1140 cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgattt tgaccttgac 1200 atgctccccg ggtaaataac ttcgtatagt atacattata cgaagttatg gatccgcggc 1260 cgcaaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa 1320 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat 1380 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct 1440 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct 1500 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc 1560 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa 1620 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc 1680 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg 1740 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc 1800 ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccatgg ccaccgagta 1860 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc 1920 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga 1980 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt 2040 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc 2100 gggggcggtg ttcgccgaga tcggctcgcg catggccgag ttgagcggtt cccggctggc 2160 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt 2220 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt 2280 gctccccgga gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg agacctccgc 2340 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt 2400 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag ctaagcacaa 2460 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 2520 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct 2580 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 2640 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 2700 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 2760 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 2820 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 2880 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 2940 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact 3000 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 3060 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 3120 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt 3180 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 3240 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 3300 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta 3360 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 3420 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 3480 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 3540 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 3600 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 3660 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 3720 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta 3780 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc 3840 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 3900 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 3960 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 4020 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 4080 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 4140 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact 4200 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 4260 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 4320 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 4380 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 4440 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg 4500 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 4560 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 4620 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 4680 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 4740 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 4800 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 4860 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 4920 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 4980 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 5040 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 5100 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 5160 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 5220 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 5280 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 5340 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 5400 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 5460 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 5520 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 5580 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 5640 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 5700 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct 5760 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 5820 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt 5880 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac 5940 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca 6000 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg 6060 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg 6120 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg 6180 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga 6240 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 6300 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 6360 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 6420 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct 6480 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg 6540 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg 6600 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg 6660 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg 6720 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac 6780 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc 6840 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag 6900 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct 6960 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 7020 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 7080 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 7140 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 7200 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 7260 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 7320 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 7380 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 7440 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 7500 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 7560 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 7620 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 7680 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 7740 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 7800 ggatctcgac ggtatcgatg gtccgaggtt ctagacgagt ttactcccta tcagtgatag 7860 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct 7920 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg 7980 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag 8040 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg 8100 aaccgtcaga tcgcaccggt cactagtcca gtgtggtgga attctgcaga tatcaacaag 8160 tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atggaagatg ccaaaaacat 8220 taagaagggc ccagcgccat tctacccact cgaagacggg accgccggcg agcagctgca 8280 caaagccatg aagcgctacg ccctggtgcc cggcaccatc gcctttaccg acgcacatat 8340 cgaggtggac attacctacg ccgagtactt cgagatgagc gttcggctgg cagaagctat 8400 gaagcgctat gggctgaata caaaccatcg gatcgtggtg tgcagcgaga atagcttgca 8460 gttcttcatg cccgtgttgg gtgccctgtt catcggtgtg gctgtggccc cagctaacga 8520 catctacaac gagcgcgagc tgctgaacag catgggcatc agccagccca ccgtcgtatt 8580 cgtgagcaag aaagggctgc aaaagatcct caacgtgcaa aagaagctac cgatcataca 8640 aaagatcatc atcatggata gcaagaccga ctaccagggc ttccaaagca tgtacacctt 8700 cgtgacttcc catttgccac ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga 8760 ccgggacaaa accatcgccc tgatcatgaa cagtagtggc agtaccggat tgcccaaggg 8820 cgtagcccta ccgcaccgca ccgcttgtgt ccgattcagt catgcccgcg accccatctt 8880 cggcaaccag atcatccccg acaccgctat cctcagcgtg gtgccatttc accacggctt 8940 cggcatgttc accacgctgg gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg 9000 cttcgaggag gagctattct tgcgcagctt gcaagactat aagattcaat ctgccctgct 9060 ggtgcccaca ctatttagct tcttcgctaa gagcactctc atcgacaagt acgacctaag 9120 caacttgcac gagatcgcca gcggcggggc gccgctcagc aaggaggtag gtgaggccgt 9180 ggccaaacgc ttccacctac caggcatccg ccagggctac ggcctgacag aaacaaccag 9240 cgccattctg atcacccccg aaggggacga caagcctggc gcagtaggca aggtggtgcc 9300 cttcttcgag gctaaggtgg tggacttgga caccggtaag acactgggtg tgaaccagcg 9360 cggcgagctg tgcgtccgtg gccccatgat catgagcggc tacgttaaca accccgaggc 9420 tacaaacgct ctcatcgaca aggacggctg gctgcacagc ggcgacatcg cctactggga 9480 cgaggacgag cacttcttca tcgtggaccg gctgaagagc ctgatcaaat acaagggcta 9540 ccaggtagcc ccagccgaac tggagagcat cctgctgcaa caccccaaca tcttcgacgc 9600 cggggtcgcc ggcctgcccg acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct 9660 ggaacacggt aaaaccatga ccgagaagga gatcgtggac tatgtggcca gccaggttac 9720 aaccgccaag aagctgcgcg gtggtgttgt gttcgtggac gaggtgccta aaggactgac 9780 cggcaagttg gacgcccgca agatccgcga gattctcatt aaggccaaga agggcggcaa 9840 gatcgccgtg tctagaaagg gtgggcgcgc cgacccagct ttc 9883 //