LOCUS       pMH30                   8824 bp    DNA     circular     29-NOV-2012
DEFINITION  LMBP 7957.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 7957
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 29-NOV-2012
COMMENT     VNTDATE|643984017|
COMMENT     VNTNAME|pMH30|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
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COMMENT        #2=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 5 3 0 3822 0 5709 0 7535 0 
COMMENT            8579 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 4 1152 0 1937 0 3927 0 
COMMENT            5909 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1 3046 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 2 6080 0 7092 0 0 "")) 
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COMMENT                                   "{\\i Hae}II (7535)" "@N (@S)" 5 -13.2713 
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COMMENT                            1.74233 0.611111 0 "Last modified: 29-NOV-2012" 
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COMMENT       (CStringList) 1094795585 1195852099 
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COMMENT        #237=(CSampFrag "pMH30" 3819 3 "HaeII" 3822 "HaeII" (LOGPEN 0 0 0) 
COMMENT              1.39837e-316 1.79256e+035) 
COMMENT        #238=(CSampFrag "pMH30" 1887 3822 "HaeII" 5709 "HaeII" (LOGPEN 0 0 0) 
COMMENT              1.19778e-316 6.19294e-310) 
COMMENT        #239=(CSampFrag "pMH30" 1826 5709 "HaeII" 7535 "HaeII" (LOGPEN 0 0 0) 
COMMENT              2.15278e-316 1.02885e-312) 
COMMENT        #240=(CSampFrag "pMH30" 1044 7535 "HaeII" 8579 "HaeII" (LOGPEN 0 0 0) 
COMMENT              1.3768e-316 1.00128e-307) 
COMMENT        #241=(CSampFrag "pMH30" 248 8579 "HaeII" 3 "HaeII" (LOGPEN 0 0 0) 
COMMENT              6.12384e-062 4.25195e-310))))
FEATURES             Location/Qualifiers
     CDS             complement(7912..8706)
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     rep_origin      complement(6662..7047)
                     /vntifkey="33"
                     /label=R6K\ori
     terminator      5971..6014
                     /vntifkey="43"
                     /label=T1\terminator
     attenuator      complement(6139..6370)
                     /vntifkey="0"
                     /label=attP3
     CDS             138..1580
                     /vntifkey="4"
                     /label=luxC
     CDS             1592..2515
                     /vntifkey="4"
                     /label=luxD
     CDS             2564..3646
                     /vntifkey="4"
                     /label=luxA
     CDS             3661..4644
                     /vntifkey="4"
                     /label=luxB
     CDS             4823..5935
                     /vntifkey="4"
                     /label=luxE
BASE COUNT     2648 a      1673 c      1933 g      2570 t 
ORIGIN
        1 gccccagctg gcaattccga cgtctaagaa accattatta tcatgacatt aacctataaa 
       61 aataggcgta tcacgaggcc ctttcgtctt cacctcgagg ggatcctcta gttgcggccg 
      121 caaaatggat ggcaaatatg actaaaaaaa tttcattcat tattaacggc caggttgaaa 
      181 tctttcccga aagtgatgat ttagtgcaat ccattaattt tggtgataat agtgtttacc 
      241 tgccaatatt gaatgactct catgtaaaaa acattattga ttgtaatgga aataacgaat 
      301 tacggttgca taacattgtc aattttctct atacggtagg gcaaagatgg aaaaatgaag 
      361 aatactcaag acgcaggaca tacattcgtg acttaaaaaa atatatggga tattcagaag 
      421 aaatggctaa gctagaggcc aattggatat ctatgatttt atgttctaaa ggcggccttt 
      481 atgatgttgt agaaaatgaa cttggttctc gccatatcat ggatgaatgg ctacctcagg 
      541 atgaaagtta tgttcgggct tttccgaaag gtaaatctgt acatctgttg gcaggtaatg 
      601 ttccattatc tgggatcatg tctatattac gcgcaatttt aactaagaat cagtgtatta 
      661 taaaaacatc gtcaaccgat ccttttaccg ctaatgcatt agcgttaagt tttattgatg 
      721 tagaccctaa tcatccgata acgcgctctt tatctgttat atattggccc caccaaggtg 
      781 atacatcact cgcaaaagaa attatgcgac atgcggatgt tattgtcgct tggggagggc 
      841 cagatgcgat taattgggcg gtagagcatg cgccatctta tgctgatgtg attaaatttg 
      901 gttctaaaaa gagtctttgc attatcgata atcctgttga tttgacgtcc gcagcgacag 
      961 gtgcggctca tgatgtttgt ttttacgatc agcgagcttg tttttctgcc caaaacatat 
     1021 attacatggg aaatcattat gaggaattta agttagcgtt gatagaaaaa cttaatctat 
     1081 atgcgcatat attaccgaat gccaaaaaag attttgatga aaaggcggcc tattctttag 
     1141 ttcaaaaaga aagcttgttt gctggattaa aagtagaggt ggatattcat caacgttgga 
     1201 tgattattga gtcaaatgca ggtgtggaat ttaatcaacc acttggcaga tgtgtgtacc 
     1261 ttcatcacgt cgataatatt gagcaaatat tgccttatgt tcaaaaaaat aagacgcaaa 
     1321 ccatatctat ttttccttgg gagtcatcat ttaaatatcg agatgcgtta gcattaaaag 
     1381 gtgcggaaag gattgtagaa gcaggaatga ataacatatt tcgagttggt ggatctcatg 
     1441 acggaatgag accgttgcaa cgattagtga catatatttc tcatgaaagg ccatctaact 
     1501 atacggctaa ggatgttgcg gttgaaatag aacagactcg attcctggaa gaagataagt 
     1561 tccttgtatt tgtcccataa taggtaaaag tatggaaaat gaatcaaaat ataaaaccat 
     1621 cgaccacgtt atttgtgttg aaggaaataa aaaaattcat gtttgggaaa cgctgccaga 
     1681 agaaaacagc ccaaagagaa agaatgccat tattattgcg tctggttttg cccgcaggat 
     1741 ggatcatttt gctggtctgg cggaatattt atcgcggaat ggatttcatg tgatccgcta 
     1801 tgattcgctt caccacgttg gattgagttc agggacaatt gatgaattta caatgtctat 
     1861 aggaaagcag agcttgttag cagtggttga ttggttaact acacgaaaaa taaataactt 
     1921 cggtatgttg gcttcaagct tatctgcgcg gatagcttat gcaagcctat ctgaaatcaa 
     1981 tgcttcgttt ttaatcaccg cagtcggtgt tgttaactta agatattctc ttgaaagagc 
     2041 tttagggttt gattatctca gtctacccat taatgaattg ccggataatc tagattttga 
     2101 aggccataaa ttgggtgctg aagtctttgc gagagattgt cttgattttg gttgggaaga 
     2161 tttagcttct acaattaata acatgatgta tcttgatata ccgtttattg cttttactgc 
     2221 aaataacgat aattgggtca agcaagatga agttatcaca ttgttatcaa atattcgtag 
     2281 taatcgatgc aagatatatt ctttgttagg aagttcgcat gacttgagtg aaaatttagt 
     2341 ggtcctgcgc aatttttatc aatcggttac gaaagccgct atcgcgatgg ataatgatca 
     2401 tctggatatt gatgttgata ttactgaacc gtcatttgaa catttaacta ttgcgacagt 
     2461 caatgaacgc cgaatgagaa ttgagattga aaatcaagca atttctctgt cttaaaatct 
     2521 attgagatat tctatcactc aaatagcaat ataaggactc tctatgaaat ttggaaactt 
     2581 tttgcttaca taccaacctc cccaattttc tcaaacagag gtaatgaaac gtttggttaa 
     2641 attaggtcgc atctctgagg agtgtggttt tgataccgta tggttactgg agcatcattt 
     2701 cacggagttt ggtttgcttg gtaaccctta tgtcgctgct gcatatttac ttggcgcgac 
     2761 taaaaaattg aatgtaggaa ctgccgctat tgttcttccc acagcccatc cagtacgcca 
     2821 acttgaagat gtgaatttat tggatcaaat gtcaaaagga cgatttcggt ttggtatttg 
     2881 ccgagggctt tacaacaagg actttcgcgt attcggcaca gatatgaata acagtcgcgc 
     2941 cttagcggaa tgctggtacg ggctgataaa gaatggcatg acagagggat atatggaagc 
     3001 tgataatgaa catatcaagt tccataaggt aaaagtaaac cccgcggcgt atagcagagg 
     3061 tggcgcaccg gtttatgtgg tggctgaatc agcttcgacg actgagtggg ctgctcaatt 
     3121 tggcctaccg atgatattaa gttggattat aaatactaac gaaaagaaag cacaacttga 
     3181 gctttataat gaagtggctc aagaatatgg gcacgatatt cataatatcg accattgctt 
     3241 atcatatata acatctgtag atcatgactc aattaaagcg aaagagattt gccggaaatt 
     3301 tctggggcat tggtatgatt cttatgtgaa tgctacgact atttttgatg attcagacca 
     3361 aacaagaggt tatgatttca ataaagggca gtggcgtgac tttgtattaa aaggacataa 
     3421 agatactaat cgccgtattg attacagtta cgaaatcaat cccgtgggaa cgccgcagga 
     3481 atgtattgac ataattcaaa aagacattga tgctacagga atatcaaata tttgttgtgg 
     3541 atttgaagct aatggaacag tagacgaaat tattgcttcc atgaagctct tccagtctga 
     3601 tgtcatgcca tttcttaaag aaaaacaacg ttcgctatta tattagctaa ggagaaagaa 
     3661 atgaaatttg gattgttctt ccttaacttc atcaattcaa caactgttca agaacaaagt 
     3721 atagttcgca tgcaggaaat aacggagtat gttgataagt tgaattttga acagatttta 
     3781 gtgtatgaaa atcatttttc agataatggt gttgtcggcg ctcctctgac tgtttctggt 
     3841 tttctgctcg gtttaacaga gaaaattaaa attggttcat taaatcacat cattacaact 
     3901 catcatcctg tcgccatagc ggaggaagct tgcttattgg atcagttaag tgaagggaga 
     3961 tttattttag ggtttagtga ttgcgaaaaa aaagatgaaa tgcatttttt taatcgcccg 
     4021 gttgaatatc aacagcaact atttgaagag tgttatgaaa tcattaacga tgctttaaca 
     4081 acaggctatt gtaatccaga taacgatttt tatagcttcc ctaaaatatc tgtaaatccc 
     4141 catgcttata cgccaggcgg acctcggaaa tatgtaacag caaccagtca tcatattgtt 
     4201 gagtgggcgg ccaaaaaagg tattcctctc atctttaagt gggatgattc taatgatgtt 
     4261 agatatgaat atgctgaaag atataaagcc gttgcggata aatatgacgt tgacctatca 
     4321 gagatagacc atcagttaat gatattagtt aactataacg aagatagtaa taaagctaaa 
     4381 caagagacgc gtgcatttat tagtgattat gttcttgaaa tgcaccctaa tgaaaatttc 
     4441 gaaaataaac ttgaagaaat aattgcagaa aacgctgtcg gaaattatac ggagtgtata 
     4501 actgcggcta agttggcaat tgaaaagtgt ggtgcgaaaa gtgtattgct gtcctttgaa 
     4561 ccaatgaatg atttgatgag ccaaaaaaat gtaatcaata ttgttgatga taatattaag 
     4621 aagtaccaca tggaatatac ctaatagatt tcgagttgca gcgaggcggc aagtgaacga 
     4681 atccccagga gcatagataa ctatgtgact ggggtgagtg aaagcagcca acaaagcagc 
     4741 agcttgaaag atgaagggta taaaagagta tgacagcagt gctgccatac tttctaatat 
     4801 tatcttgagg agtaaaacag gtatgacttc atatgttgat aaacaagaaa ttacagcaag 
     4861 ctcagaaatt gatgatttga ttttttcgag cgatccatta gtgtggtctt acgacgagca 
     4921 ggaaaaaatc agaaagaaac ttgtgcttga tgcatttcgt aatcattata aacattgtcg 
     4981 agaatatcgt cactactgtc aggcacacaa agtagatgac aatattacgg aaattgatga 
     5041 catacctgta ttcccaacat cggtttttaa gtttactcgc ttattaactt ctcaggaaaa 
     5101 cgagattgaa agttggttta ccagtagcgg cacgaatggt ttaaaaagtc aggtggcgcg 
     5161 tgacagatta agtattgaga gactcttagg ctctgtgagt tatggcatga aatatgttgg 
     5221 tagttggttt gatcatcaaa tagaattagt caatttggga ccagatagat ttaatgctca 
     5281 taatatttgg tttaaatatg ttatgagttt ggtggaattg ttatatccta cgacatttac 
     5341 cgtaacagaa gaacgaatag attttgttaa aacattgaat agtcttgaac gaataaaaaa 
     5401 tcaagggaaa gatctttgtc ttattggttc gccatacttt atttatttac tctgccatta 
     5461 tatgaaagat aaaaaaatct cattttctgg agataaaagc ctttatatca taaccggagg 
     5521 cggctggaaa agttacgaaa aagaatctct gaaacgtgat gatttcaatc atcttttatt 
     5581 tgatactttc aatctcagtg atattagtca gatccgagat atatttaatc aagttgaact 
     5641 caacacttgt ttctttgagg atgaaatgca gcgtaaacat gttccgccgt gggtatatgc 
     5701 gcgagcgctt gatcctgaaa cgttgaaacc tgtacctgat ggaacgccgg ggttgatgag 
     5761 ttatatggat gcgtcagcaa ccagttatcc agcatttatt gttaccgatg atgtcgggat 
     5821 aattagcaga gaatatggta agtatcccgg cgtgctcgtt gaaattttac gtcgcgtcaa 
     5881 tacgaggacg cagaaagggt gtgctttaag cttaaccgaa gcgtttgata gttgatatcc 
     5941 tttgcctgcg gccgcaacta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg 
     6001 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg 
     6061 ccctagacct agccttaatt aaccatggca tcacagtatc gtgatgacag aggcagggag 
     6121 tgggacaaaa ttgaaatcaa ataatgattt tattttgact gatagtgacc tgttcgttgc 
     6181 aacaaattga taagcaatgc ttttttataa tgccaactta gtataaaaaa gctgaacgag 
     6241 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta 
     6301 cataatactg taaaacacaa catatgcagt cactatgaat caactactta gatggtatta 
     6361 gtgacctgta acagagcatt agcgcaaggt gtttttgtct tcttgcgcta attttttgtc 
     6421 atcaaacctg tcgcactcca gagaagcaca aagcctcgca atccagtgca aagctgaatt 
     6481 ggggatcatt tattctgcct cccagagcct gataaaaacg gtgaatccgt tagcgaggtg 
     6541 ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgattat tgaagcattt 
     6601 atcagggtta ttgtctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 
     6661 catgtcagcc gttaagtgtt cctgtgtcac tgaaaattgc tttgagaggc tctaagggct 
     6721 tctcagtgcg ttacatccct ggcttgttgt ccacaaccgt taaaccttaa aagctagctt 
     6781 taaaagcctt atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag 
     6841 ttgctgattt atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga 
     6901 gcttagtacg ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac 
     6961 atgagagctt agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg 
     7021 tactatcaac aggttgaact gctgatcgat ctcacatgtt ctttcctgcg ttatcccctg 
     7081 attctgttaa ttaaggtcct tttcctttga gttgtgggta tctgtaaatt ctgctagacc 
     7141 tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag acctttgtgt 
     7201 gttttttttg tttatattca agtggttata atttatagaa taaagaaaga ataaaaaaag 
     7261 ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt tccgcagtat 
     7321 tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta aaaccctaaa 
     7381 ggcttaagta gcaccctcgc aagctcgggc aaatcgctga atattccttt tgtctccgac 
     7441 catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc tcacggctct 
     7501 ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg caaggaaact 
     7561 acccataata caagaaaagc ccgtcacggg cttctcaggg cgttttatgg cgggtctgct 
     7621 atgtggtgct atctgacttt ttgctgttca gcagttcctg ccctctgatt ttccagtctg 
     7681 accacttcgg attatcccgt gacaggtcat tcagactggc taatgcaccc agtaaggcag 
     7741 cggtatcatc aacaggctta cccgtcttac tgtccctagt gcttggattc tcaccaataa 
     7801 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta 
     7861 ctggatctat caacaggagt ccaagcgagc tctcgaaccc cagagtcccg ctcagaagaa 
     7921 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag 
     7981 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa 
     8041 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa 
     8101 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc 
     8161 ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg 
     8221 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg 
     8281 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag 
     8341 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag 
     8401 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac 
     8461 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc 
     8521 gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc 
     8581 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc 
     8641 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc 
     8701 aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc tgcgccatca 
     8761 gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga 
     8821 gggc 
//
