LOCUS       gGFP                    9240 bp    DNA     circular     28-MAY-2013
DEFINITION  LMBP 8052.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8052
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-MAY-2013
            
COMMENT     VNTDATE|663265412|
COMMENT     VNTNAME|gGFP|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "gGFP" 0 9240 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "BglII" "EcoRI" "PvuI" "PvuII" "SalI" "NcoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 752 0 4702 0 6043 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BglII" "agatct" 2 0 1 2 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1802 0 6843 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 1179 0 6728 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PvuII" "cagctg" 4 0 2 1858 0 7286 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 2895 0 7438 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 2538 0 5452 0 6734 0 0 "")) 
COMMENT       (CObList 
COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 0 760 1620 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "689..1549") 
COMMENT        #8=(CFSignal (CObList) "pMB1" 33 0 0 17 419 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "7..409") 
COMMENT        #9=(CFSignal (CObList) "SGFP" 4 0 1 1817 2533 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "hygromycin resistance" 4 0 1 6065 7084 0 
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COMMENT        #11=(CFSignal (CObList) "trpC terminator" 43 0 1 5331 5893 0 
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COMMENT        #12=(CFSignal (CObList) "gpdA promoter" 29 0 1 7257 9240 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "gpdA promoter" 29 0 1 2713 3860 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'gGFP'" 1) 
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COMMENT                          "Local object" 1) 
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COMMENT                          "Created: 05/28/13 04:23" 1) 
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COMMENT                          "Last Modified: 05/28/13 05:53" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9240 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 22-MAY-2013" 1))) 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                   "Feature Map") 
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FEATURES             Location/Qualifiers
     CDS             760..1620
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      17..419
                     /vntifkey="33"
                     /label=pMB1
     CDS             complement(1817..2533)
                     /vntifkey="4"
                     /label=SGFP
     CDS             complement(6065..7084)
                     /vntifkey="4"
                     /label=hygromycin\resistance
     promoter        complement(7257..9240)
                     /vntifkey="29"
                     /label=gpdA\promoter
     promoter        complement(2713..3860)
                     /vntifkey="29"
                     /label=gpdA\promoter
     terminator      complement(5331..5893)
                     /vntifkey="43"
                     /label=trpC\terminator
BASE COUNT     1798 a      1778 c      1851 g      1818 t      1995 others
ORIGIN
        1 agatctnnnn nnnnnngctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 
       61 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 
      121 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 
      181 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 
      241 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 
      301 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 
      361 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagn 
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggatccnnna tgagtattca acatttccgt 
      781 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 
      841 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 
      901 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 
      961 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 
     1021 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 
     1081 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 
     1141 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 
     1201 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 
     1261 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 
     1321 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 
     1381 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 
     1441 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 
     1501 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 
     1561 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1801 gaattcnnnn nnnnnnttat ttgtatagtt catccatgcc atgtgtaatc ccagcagctg 
     1861 ttacaaactc aagaaggacc atgtggtctc tcttttcgtt gggatctttc gaaagggcag 
     1921 attgtgtgga caggtaatgg ttgtctggta aaaggacagg gccatcgcca attggagtat 
     1981 tttgttgata atggtctgct agttgaacgc ttccatcttc aatgttgtgt ctaattttga 
     2041 agttaacttt gattccattc ttttgtttgt ctgccatgat gtatacattg tgtgagttat 
     2101 agttgtattc caatttgtgt ccaagaatgt ttccatcttc tttaaaatca atacctttta 
     2161 actcgattct attaacaagg gtatcacctt caaacttgac ttcagcacgt gtcttgtagt 
     2221 tcccgtcatc tttgaaaaat atagttcttt cctgtacata accttcgggc atggcactct 
     2281 tgaaaaagtc atgccgtttc atatgatctg ggtatcttga aaagcattga acaccataag 
     2341 tgaaagtagt gacaagtgtt ggccaaggaa caggtagttt tccagtagtg caaataaatt 
     2401 taagggtaag ttttccgtat gttgcatcac cttcaccctc tccactgaca gaaaatttgt 
     2461 gcccattaac atcaccatct aattcaacaa gaattgggac aactccagtg aaaagttctt 
     2521 ctcctttact catnnnccat ggtgatgtct gctcaagcgg ggtagctgtt agtcaagctg 
     2581 cgatgaagtg ggaaagctcg aactgaaagg ttcaaaggaa taagggatgg gaaggatgga 
     2641 gtatggatgt agcaaagtac ttacttaggg gaaataaagg ttcttggatg ggaagatgaa 
     2701 tatactgaag atgggaaaag aaagagaaaa gaaaagagca gnctggtggg gagagcagga 
     2761 aaatatggca acaaatgttg gactgacgca acgaccttgt caaccccgcc gacacaccgg 
     2821 gcggacagac ggggcaaagc tgcctaccag ggactgaggg acctcagcag gtcgagtgca 
     2881 gagcaccgga tgggtcgact gccagcttgt gttcccggtc tgcgccgctg gccagctcct 
     2941 gagcggcctt tccggtttca tacaccgggc aaagcaggag aggcacgata tttggacgcc 
     3001 ctacagatgc cggatgggcc aattagggag cttacgcgcc gggtactcgc tctacctact 
     3061 tcggagaagg tactatctcg tgaatctttt accagatcgg aagcaattgg acttctgtac 
     3121 ctaggttaat ggcatgctat ttcgccgacg gctatacacc cctggcttca cattctcctt 
     3181 cgcttactgc cggtgattcg atgaagctcc atattctccg atgatgcaat agattcttgg 
     3241 tcaacgaggg gcacaccagc ctttccactt cggggcggag gggcggccgg tcccggatta 
     3301 ataatcatcc actgcacctc agagccgcca gagctgtctg gccagtggct tattactcag 
     3361 cccttctctc tgcgtccgtc cgtctctccg catgccagaa agagtcaccg gtcactgtac 
     3421 agagctcacg agttcgtcac atttttctac aaatggtgga ggcggcggat tttaggctca 
     3481 agtcatgacc ctctgggtca ctccagaatc agctaggtca acgaataagg atgattctat 
     3541 aggaagatcc aggcaccggt caaccatgat ctggacagat ttgggagctc ggtataagct 
     3601 ctccacctat cttattctgt atagtttagg cttaaagttt atccaggaga tgttgctgaa 
     3661 gtcgatttga gtccacttcc tcactggtag ctatacgact ttgatggtcg ttgtaggggc 
     3721 tgtattaggt ctcgatcaaa cacaaataga attaaatggt actcgagtcc actgaaggtg 
     3781 gcttctccgt cttccgtagc cgtgccgaaa tccttacagc ttgtgttgtg tgacttttgg 
     3841 ttagcgctct gactttgtgg aggcctannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4681 nnnnnnnnnn nnnnnnnnnn ggatccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctagaaagaa 
     5341 ggattacctc taaacaagtg tacctgtgca ttctgggtaa acgactcata ggagagttgt 
     5401 aaaaaagttt cggccggcgt attgggtgtt acggagcatt cactaggcaa ccatggttac 
     5461 tattgtatac catcttagta ggaatgattt cgaggtttat acctacgatg aatgtgtgtc 
     5521 ctgtaggctt gagagttcaa ggaagaaaca tgcaattatc tttgcgaacc cagggctggt 
     5581 gacggaattt tcatagtcaa gctatcagag taaagaagag gagcatgtca aagtacaatt 
     5641 agagacaaat atatagtcgc gtggagccaa gagcggattc ctcagtctcg taggtctctt 
     5701 gacgaccgtt gatctgcttg atctcgtctc ccgaaaatga aaatagctct gctaagctat 
     5761 tcttctcttc gccggagcct gaaggcgtta ctaggttgca gtcaatgcat taatgcattg 
     5821 cagatgagct gtatctggaa gaggtaaacc cgaaaacgcg ttttattctt gttgacatgg 
     5881 agctattaaa tcactagaag gcactctttg ctgcttggac aaatgaacgt atcttatcga 
     5941 gatcctgaac accatttgtc tcaactccgg agctgacatc gacaccaacg atcttatatc 
     6001 cagattcgtc aagctgtttg atgatttcag taacgttaag tggatcccgc ggtcggcatc 
     6061 tactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta 
     6121 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga 
     6181 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat 
     6241 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg 
     6301 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct 
     6361 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat 
     6421 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga 
     6481 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc 
     6541 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag 
     6601 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt 
     6661 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg 
     6721 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag 
     6781 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc 
     6841 tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat 
     6901 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac 
     6961 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt 
     7021 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag 
     7081 gcatggtgat gtctgctcaa gcggggtagc tgttagtcaa gctgcgatga agtgggaaag 
     7141 ctcgaactga aaggttcaaa ggaataaggg atgggaagga tggagtatgg atgtagcaaa 
     7201 gtacttactt aggggaaata aaggttcttg gatgggaaga tgaatatact gaagatggga 
     7261 aaagaaagag aaaagaaaag agcagctggt ggggagagca ggaaaatatg gcaacaaatg 
     7321 ttggactgac gcaacgacct tgtcaacccc gccgacacac cgggcggaca gacggggcaa 
     7381 agctgcctac cagggactga gggacctcag caggtcgagt gcagagcacc ggatgggtcg 
     7441 actgccagct tgtgttcccg gtctgcgccg ctggccagct cctgagcggc ctttccggtt 
     7501 tcatacaccg ggcaaagcag gagaggcacg atatttggac gccctacaga tgccggatgg 
     7561 gccaattagg gagcttacgc gccgggtact cgctctacct acttcggaga aggtactatc 
     7621 tcgtgaatct tttaccagat cggaagcaat tggacttctg tacctaggtt aatggcatgc 
     7681 tatttcgccg acggctatac acccctggct tcacattctc cttcgcttac tgccggtgat 
     7741 tcgatgaagc tccatattct ccgatgatgc aatagattct tggtcaacga ggggcacacc 
     7801 agcctttcca cttcggggcg gaggggcggc cggtcccgga ttaataatca tccactgcac 
     7861 ctcagagccg ccagagctgt ctggccagtg gcttattact cagcccttct ctctgcgtcc 
     7921 gtccgtctct ccgcatgcca gaaagagtca ccggtcactg tacagagctc acgagttcgt 
     7981 cacatttttc tacaaatggt ggaggcggcg gattttaggc tcaagtcatg accctctggg 
     8041 tcactccaga atcagctagg tcaacgaata aggatgattc tataggaaga tccaggcacc 
     8101 ggtcaaccat gatctggaca gatttgggag ctcggtataa gctctccacc tatcttattc 
     8161 tgtatagttt aggcttaaag tttatccagg agatgttgct gaagtcgatt tgagtccact 
     8221 tcctcactgg tagctatacg actttgatgg tcgttgtagg ggctgtatta ggtctcgatc 
     8281 aaacacaaat agaattaaat ggtactcgag tccactgaag gtggcttctc cgtcttccgt 
     8341 agccgtgccg aaatccttac agcttgtgtt gtgtgacttt tggttagcgc tctgactttg 
     8401 tggtgagcta actagagatc atgctatatc tcctgattta atacaatgct catcataaca 
     8461 ttccacctgg aactgctagc aacgtttgac ttgcattgtg caacgccctt tgcagagcta 
     8521 tcggatgatc aatagtgcca cgttctaaat tcaaccaacg caggtgcccc aagccttcga 
     8581 catccggatg tatttcgaaa acctcatggc gattgcagtc ctcagattca tgttcattcc 
     8641 aatgctcatt ggtgaataaa aggttcacag ggaataagtt caaactcgag atacttgaga 
     8701 atattgaaag ccaaaggacc ctctatgctc caagctagag tctcagcctg gaaagcaaat 
     8761 ccaaatgaag ctatgctacc tccaattcct catcatctta tctataatac agagtcgaag 
     8821 aatatcctct tgacaccgct ccgtcctccg acttcaataa ggagcttact cctccttgac 
     8881 accacccctc cagttcttct cggcgttctg gagggaggcc ttgtcggtct tgggctggcc 
     8941 ctggctgaga aagctgttgg cagccttaaa gggacgctgg aggtcaccag tcgctggctt 
     9001 cccgaagacg tggatcttaa ccagattcga aagcgccttc agcggatgat cgactggatc 
     9061 agaagagcgt tggtgtactt gaagtacaga tgcatgacgg ccatcatgcc aacgcccatg 
     9121 aactggctct taatgagctg gcggaactgg cccttatcgt actccatgtt ggtagttgtg 
     9181 acaggacgag gctcctcgcc gcttccaagc ggagcaggct cgacgtattt cagtgtcgaa 
//
