LOCUS       pENTR3c-SHP1-WT           4077 bp    DNA     circular SYN  6-JAN-2011
DEFINITION  LMBP 8068.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|578419893|
COMMENT     VNTNAME|pENTR3c-SHP1-WT|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pENTR3c-SHP1-WT" 0 4077 
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COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
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COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI" "XhoI") (CStringList "atg" "gtg") 
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COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
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COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 57 0 350 0 1394 0 1557 0 
COMMENT            1601 0 1990 0 2293 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 485 0 663 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 497 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 504 0 838 0 1762 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 6 765 0 879 0 1374 0 1865 0 
COMMENT            2085 0 2419 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 1396 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 1394 0 0 "") 
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COMMENT        #14=(CFSignal (CObList) "pUC origin" 33 0 0 3401 4074 0 (CStringList) 
COMMENT        #15=(CFSignal (CObList) "rrnB T1 transcription terminator" 43 0 1 106 
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COMMENT        #16=(CFSignal (CObList) "rrnB T2 transcription terminator" 43 0 1 281 
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COMMENT        #17=(CFSignal (CObList) "SHP-1 WT" 21 0 0 505 2289 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: Invitrogen" 
COMMENT                          1) 
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COMMENT                          "Created: 01/06/11 04:11" 1) 
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COMMENT                          "Last Modified: 01/06/11 04:37" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4077 bp" 1) 
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COMMENT                          "CYCGRG" "GRGCYC") 
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FEATURES             Location/Qualifiers
     source          1..4077
                     /vntifkey="98"
     misc_recomb     358..457
                     /vntifkey="86"
                     /label=attL1
     misc_recomb     complement(2308..2405)
                     /vntifkey="86"
                     /label=attL2
     CDS             2528..3337
                     /vntifkey="4"
                     /label=Kan(R)
     rep_origin      3401..4074
                     /vntifkey="33"
                     /label=pUC\origin
     terminator      complement(106..149)
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     terminator      complement(281..308)
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     misc_feature    505..2289
                     /vntifkey="21"
                     /label=SHP-1\WT
     mutation        1861..1863
                     /vntifkey="62"
                     /label=Ser\in\SHP-1\C453S\inactivate\mutant
BASE COUNT     1030 a      1007 c      1104 g       936 t 
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg 
      481 actggatccg gtaccgaatt caccatggtg aggtggtttc accgagacct cagtgggctg 
      541 gatgcagaga ccctgctcaa gggccgaggt gtccacggta gcttcctggc tcggcccagt 
      601 cgcaagaacc agggtgactt ctcgctctcc gtcagggtgg gggatcaggt gacccatatt 
      661 cggatccaga actcagggga tttctatgac ctgtatggag gggagaagtt tgcgactctg 
      721 acagagctgg tggagtacta cactcagcag cagggtgtcc tgcaggaccg cgacggcacc 
      781 atcatccacc tcaagtaccc gctgaactgc tccgatccca ctagtgagag gtggtaccat 
      841 ggccacatgt ctggcgggca ggcagagacg ctgctgcagg ccaagggcga gccctggacg 
      901 tttcttgtgc gtgagagcct cagccagcct ggagacttcg tgctttctgt gctcagtgac 
      961 cagcccaagg ctggcccagg ctccccgctc agggtcaccc acatcaaggt catgtgcgag 
     1021 ggtggacgct acacagtggg tggtttggag accttcgaca gcctcacgga cctggtggag 
     1081 catttcaaga agacggggat tgaggaggcc tcaggcgcct ttgtctacct gcggcagccg 
     1141 tactatgcca cgagggtgaa tgcggctgac attgagaacc gagtgttgga actgaacaag 
     1201 aagcaggagt ccgaggatac agccaaggct ggcttctggg aggagtttga gagtttgcag 
     1261 aagcaggagg tgaagaactt gcaccagcgt ctggaagggc agcggccaga gaacaagggc 
     1321 aagaaccgct acaagaacat tctccccttt gaccacagcc gagtgatcct gcagggacgg 
     1381 gacagtaaca tccccgggtc cgactacatc aatgccaact acatcaagaa ccagctgcta 
     1441 ggccctgatg agaacgctaa gacctacatc gccagccagg gctgtctgga ggccacggtc 
     1501 aatgacttct ggcagatggc gtggcaggag aacagccgtg tcatcgtcat gaccacccga 
     1561 gaggtggaga aaggccggaa caaatgcgtc ccatactggc ccgaggtggg catgcagcgt 
     1621 gcttatgggc cctactctgt gaccaactgc ggggagcatg acacaaccga atacaaactc 
     1681 cgtaccttac aggtctcccc gctggacaat ggagacctga ttcgggagat ctggcattac 
     1741 cagtacctga gctggcccga ccatggggtc cccagtgagc ctgggggtgt cctcagcttc 
     1801 ctggaccaga tcaaccagcg gcaggaaagt ctgcctcacg cagggcccat catcgtgcac 
     1861 tgcagcgccg gcatcggccg cacaggcacc atcattgtca tcgacatgct catggagaac 
     1921 atctccacca agggcctgga ctgtgacatt gacatccaga agaccatcca gatggtgcgg 
     1981 gcgcagcgct cgggcatggt gcagacggag gcgcagtaca agttcatcta cgtggccatc 
     2041 gcccagttca ttgaaaccac taagaagaag ctggaggtcc tgcagtcgca gaagggccag 
     2101 gagtcggagt acgggaacat cacctatccc ccagccatga agaatgccca tgccaaggcc 
     2161 tcccgcacct cgtccaaaca caaggaggat gtgtatgaga acctgcacac taagaacaag 
     2221 agggaggaga aagtgaagaa gcagcggtca gcagacaagg agaagagcaa gggttccctc 
     2281 aagaggaagc actcgagata tctagaccca gctttcttgt acaaagttgg cattataaga 
     2341 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt 
     2401 atttgccatc cagctgcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca 
     2461 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag 
     2521 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga 
     2581 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat 
     2641 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag 
     2701 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc 
     2761 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc 
     2821 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat 
     2881 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc 
     2941 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt 
     3001 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa 
     3061 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc 
     3121 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt 
     3181 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc 
     3241 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa 
     3301 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta 
     3361 acattattca gattgggccc cgttccactg agcgtcagac cccgtagaaa agatcaaagg 
     3421 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 
     3481 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 
     3541 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca 
     3601 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 
     3661 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 
     3721 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 
     3781 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 
     3841 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 
     3901 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 
     3961 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 
     4021 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt 
//
