LOCUS       Cre_excised_pCOIN_DV-NLS-lacZ       15321 bp    DNA     circular UNA 19-DEC-2016
DEFINITION  LMBP 8174.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8174
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11552635|
COMMENT     VNTDBDATE|-11552635|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Cre_excised_pCOIN_DV-NLS-lacZ|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
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COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #4=(CFSignal (CObList) "loxP" 21 0 1 8023 8056 0 (CStringList) 
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COMMENT        #5=(CFSignal (CObList) "BGH polyA" 43 0 0 3723 3947 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) 
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COMMENT        #7=(CFSignal (CObList) 
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COMMENT        #9=(CFSignal (CObList) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 12/19/16 02:10" 1) 
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COMMENT                          "Last Modified: 12/19/16 02:10" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #130# 0 100) 
COMMENT                                   (LOGPEN 0 10 16711680) 8 0.835356 4.42575 
COMMENT                                   4.52524) 
COMMENT                             #227=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 6 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "chicken \337-globin 5'HS4 insulator core sequence" 
COMMENT                                   "@N" 1 25.0388 -4.59728 0 -10 21.2528 1.14493 
COMMENT                                   #226#) 
COMMENT                             #228=(CScratch 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #130# 936 100) 
COMMENT                                   (LOGPEN 0 10 16711680) 8 1 4.20096 1.9 
COMMENT                                   0.082322 1) 
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COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "FRT mutant" 
COMMENT                                   "@N" 1 16.5504 -8.23937 0 -10 5.26668 1.14493 
COMMENT                                   #228#) 
COMMENT                             #230=(CLine 
COMMENT                                   (CWidget 0 (3 #18# 0) 1 2 0 0 #130# 0 100) 
COMMENT                                   (LOGPEN 0 10 16711680) 8 0.835356 4.84052 
COMMENT                                   4.98628) 
COMMENT                             #231=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #168# 1619935543 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' flanking region of rabbit \337-globin" 
COMMENT                                   "@N" 1 22.6615 3.93201 0 -10 15.2848 1.14493 
COMMENT                                   #230#)) (CObjectList)) 
COMMENT                      #232=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -889 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #164# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 
COMMENT                                    0.99596 4.21038 4.41838 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "mPGK1 promoter" 
COMMENT                                   "@N" 1 18.0008 -6.48272 0 -10 7.79983 1.14493 
COMMENT                                   #233#) 
COMMENT                             #235=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #22# 0) 1 2 0 0 #164# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 
COMMENT                                    0.993079 1.48958 1.64067 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "hCMVT promoter" 
COMMENT                                   "@N" 1 -19.1858 1.12693 0 -10 7.59947 1.14493 
COMMENT                                   #235#)) (CObjectList)) 
COMMENT                      #237=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -116 100) 
COMMENT                            (CObjectList 
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COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #146# 0 100) 
COMMENT                                    (LOGPEN 0 8 128) 6 0.866337 4.14568 4.23295) 
COMMENT                                   0.4 0.082322 0.932177 1.8 1.8 1) 
COMMENT                             #239=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 6 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lac promoter" 
COMMENT                                   "@N" 1 15.7671 -9.78133 0 -10 5.65309 1.14493 
COMMENT                                   #238#)) (CObjectList)) 
COMMENT                      #240=(CGroupWidget 
COMMENT                            (CWidget 32 (7 32 0) 1 2 0 0 Nil -1030 100) 
COMMENT                            (CObjectList 
COMMENT                             #241=(CLine 
COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #144# 
COMMENT                                    1619794294 100) (LOGPEN 0 10 16711680) 8 
COMMENT                                   0.835356 6.014 6.23674) 
COMMENT                             #242=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 6 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' UTR of the EMCV polyprotein gene (IRES)" 
COMMENT                                   "@N" 1 12.3192 14.3431 0 -10 19.9647 1.14493 
COMMENT                                   #241#) 
COMMENT                             #243=(CLine 
COMMENT                                   (CWidget 0 (3 #14# 0) 1 2 0 0 #144# 1599 100) 
COMMENT                                   (LOGPEN 0 10 16711680) 8 0.835356 2.25198 
COMMENT                                   2.47472) 
COMMENT                             #244=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 6 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' UTR of the EMCV polyprotein gene (IRES)" 
COMMENT                                   "@N" 1 -19.9179 -10.261 0 -10 19.9647 1.14493 
COMMENT                                   #243#)) (CObjectList)) 
COMMENT                      #245=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -468 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #0# 0) 1 2 0 0 #139# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    3.90902 4.07403 1 0.082322) 0.8 1.8 1) 
COMMENT                             #247=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 6 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 12.3125 -11.1516 0 -10 4.12175 1.14493 
COMMENT                                   #246#)) (CObjectList)) 
COMMENT                      #248=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil 1633 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (CWidget 0 (3 #5# 0) 1 2 0 0 #130# 1022 100) 
COMMENT                                   (LOGPEN 0 10 16711680) 8 0.835356 4.65709 
COMMENT                                   4.74921) 
COMMENT                             #250=(CLabel (CWidget 0 (0 0) 1 2 0 0 #177# 8 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "BGH polyA" "@N" 
COMMENT                                   1 16.9547 -0.940879 0 -10 4.93751 1.14493 
COMMENT                                   #249#) 
COMMENT                             #251=(CScratch 
COMMENT                                   (CWidget 0 (3 #17# 0) 1 2 0 0 #130# 936 100) 
COMMENT                                   (LOGPEN 0 10 16711680) 8 1 5.02395 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #252=(CLabel (CWidget 0 (0 0) 1 2 0 0 #177# 6 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' UTR of rabbit \337-globin, incl. polyA" 
COMMENT                                   "@N" 1 22.582 5.53122 0 -10 15.9002 1.14493 
COMMENT                                   #251#) 
COMMENT                             #253=(CLine 
COMMENT                                   (CWidget 0 (3 #21# 0) 1 2 0 0 #130# 1079 100) 
COMMENT                                   (LOGPEN 0 10 16711680) 8 0.835356 1.87897 
COMMENT                                   1.97109) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "BGH polyA" "@N" 
COMMENT                                   1 -16.7956 -5.44535 0 -10 4.93751 1.14493 
COMMENT                                   #253#)) (CObjectList))) (CObjectList)) 
COMMENT               #255=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -889 100) 
COMMENT                     (CObjectList 
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COMMENT                            (LOGPEN 0 0 8388608) 1 
COMMENT                            (LOGFONT 110 41 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 8174" 
COMMENT                            "LMBP 8174" 65535 -0.100181 -2.61903 0 -10 6.91251 
COMMENT                            1.57428 Nil)) (CObjectList)) 
COMMENT               #257=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil 1687 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #258=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -538 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 61354604 
COMMENT       (CStringList) 1018 47689136 (CObList)))
FEATURES             Location/Qualifiers
     Misc._recombina complement(join(12701..12707,1..14))
                     /vntifkey="21"
                     /label=attB2
                     /note="Unknown feature type:Misc._recombina"
     rep_origin      complement(5372..5774)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(6315..7175)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    7793..7840
                     /vntifkey="21"
                     /label=FRT
     misc_feature    7857..7960
                     /vntifkey="21"
                     /label=SA
     misc_feature    complement(8023..8056)
                     /vntifkey="21"
                     /label=loxP
     terminator      3723..3947
                     /vntifkey="43"
                     /label=BGH\polyA
     misc_feature    10798..11040
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    11054..11296
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     promoter        4531..5038
                     /vntifkey="29"
                     /label=mPGK1\promoter
     misc_feature    4027..4269
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    4270..4512
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    5062..5109
                     /vntifkey="21"
                     /label=FRT\mutant
     promoter        complement(5197..5280)
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             90..633
                     /vntifkey="32"
                     /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES)
     RBS             9278..9821
                     /vntifkey="32"
                     /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES)
     CDS             646..1362
                     /vntifkey="4"
                     /label=EGFP
     CDS             1387..3036
                     /vntifkey="4"
                     /label=LUCm
     terminator      3052..3143
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_feature    3144..3499
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             8273..9277
                     /vntifkey="4"
                     /label=rtTA
     CDS             9822..10421
                     /vntifkey="4"
                     /label=puromycin\resistance
     terminator      10508..10732
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        11315..11683
                     /vntifkey="29"
                     /label=hCMVT\promoter
     attenuator      11812..11836
                     /vntifkey="0"
                     /label=attB1
     attenuator      complement(15312..15)
                     /vntifkey="0"
                     /label=attB2
     attenuator      8154..8175
                     /vntifkey="0"
                     /label=attB4
     attenuator      complement(3610..3631)
                     /vntifkey="0"
                     /label=attB3
     CDS             12235..15039
                     /vntifkey="4"
                     /label=lacZ
     CDS             11947..12234
                     /vntifkey="4"
                     /label=NLS
BASE COUNT     3570 a      4146 c      4139 g      3466 t 
ORIGIN
        1 cttgtacaaa gtggttaaag gaaccaattc agtcgagctc aagcttcgaa ttgatccgcc 
       61 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg 
      121 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga 
      181 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa 
      241 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa 
      301 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct 
      361 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg 
      421 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg 
      481 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca 
      541 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg 
      601 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc 
      661 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 
      721 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 
      781 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 
      841 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 
      901 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 
      961 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 
     1021 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 
     1081 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac 
     1141 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 
     1201 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 
     1261 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 
     1321 accgccgccg ggatcactct cggcatggac gagctgtaca agtactcaga tccaccggtc 
     1381 gccaccgaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat 
     1441 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca 
     1501 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg 
     1561 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc 
     1621 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga 
     1681 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc 
     1741 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg 
     1801 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag 
     1861 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac 
     1921 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct 
     1981 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc 
     2041 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt 
     2101 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga 
     2161 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat 
     2221 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact 
     2281 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc 
     2341 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga 
     2401 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg 
     2461 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg 
     2521 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc 
     2581 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat 
     2641 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag 
     2701 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc 
     2761 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa 
     2821 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg 
     2881 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg 
     2941 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc 
     3001 ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agagcggccg cgatcttttt 
     3061 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa 
     3121 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga 
     3181 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg 
     3241 gcaacatatg cccatatgct ggctgccatg aacaaaggtg gctataaaga ggtcatcagt 
     3301 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt 
     3361 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct 
     3421 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc 
     3481 ctcttctctt atgaagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag 
     3541 ctgtttcctg tgtgaaaggg cgaattctgc agatatccat cacactggcg gccgcactcg 
     3601 agatatctac aactttatta tacatagttg atagcttgat agcttggcgt aatcatggtc 
     3661 atagctgttt cctgtgtgaa attgttatcc gctcgggccg cgagctcgct gatcagcctc 
     3721 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac 
     3781 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg 
     3841 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga 
     3901 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga 
     3961 aagaaccagc tggggctcga tcctctagtt ggcgcgccgg gccctcgcga ctcgaggaat 
     4021 aaattcgagc tcacggggac agcccccccc caaagccccc agggatgtaa ttacgtccct 
     4081 cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc 
     4141 gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc 
     4201 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg ggatacgggg 
     4261 aaaaagcttg agctcacggg gacagccccc ccccaaagcc cccagggatg taattacgtc 
     4321 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc 
     4381 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg 
     4441 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct gggggatacg 
     4501 gggaaaaagc ttgtcgacga ataaattcaa ttctaccggg taggggaggc gcttttccca 
     4561 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc 
     4621 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt 
     4681 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag 
     4741 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg 
     4801 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt 
     4861 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg 
     4921 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg 
     4981 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc 
     5041 tgcaatcgcc gccaccatgg agaagttcct attccgaagt tcctattctt caaaaggtat 
     5101 aggaacttcc gatcgggatc cccaatcctg catgcaagct tggcgtaatc atggtcatag 
     5161 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 
     5221 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 
     5281 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 
     5341 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 
     5401 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
     5461 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
     5521 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
     5581 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
     5641 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
     5701 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 
     5761 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
     5821 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
     5881 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
     5941 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 
     6001 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
     6061 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
     6121 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
     6181 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
     6241 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
     6301 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
     6361 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
     6421 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
     6481 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
     6541 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
     6601 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 
     6661 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
     6721 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
     6781 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
     6841 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
     6901 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 
     6961 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
     7021 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
     7081 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
     7141 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 
     7201 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 
     7261 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 
     7321 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg 
     7381 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 
     7441 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 
     7501 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 
     7561 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg 
     7621 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 
     7681 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 
     7741 cagtcacgac gttgtaaaac gacggccagt gaattccaca aaagtgcgat cggaagttcc 
     7801 tattccgaag ttcctattct ctagaaagta taggaacttc ttaattaagg gatctgtagg 
     7861 gcgcagtagt ccagggtttc cttgatgatg tcatacttat cctgtccctt ttttttccac 
     7921 agctcgcggt tgaggacaaa ctcttcgcgg tctttccagt ggggatcgac ggtatcgtag 
     7981 agtcgaggcc gctctagaac tagtggatcc ggaaccctta atataacttc gtataatgta 
     8041 tgctatacga agttattagg tccctcgacc tgcagcccaa gctagcttat cgataccgtc 
     8101 gaccgagctc gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttatcaactt 
     8161 tgtatagaaa agttggctcc gaattcgccc ttttaaagga accaattcag tcgaggtcga 
     8221 cggtatcgat aagcttgata tcgaattcac catgccaaag agacccagac cctctagatt 
     8281 agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg 
     8341 tttaacaacc cgtaaactcg cccagaagct tggtgtagag cagcctacac tgtattggca 
     8401 tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca 
     8461 tactcacttt tgccctttaa aaggggaaag ctggcaagat tttttacgca ataacgctaa 
     8521 aagttttaga tgtgctttac taagtcatcg caatggagca aaagtacatt cagatacacg 
     8581 gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg 
     8641 tttttcacta gagaacgcgt tatatgcact cagcgctgtg gggcatttta ctttaggttg 
     8701 cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga 
     8761 tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc 
     8821 agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga 
     8881 aagtgggtcc gcgtacagcc gcgcgcgtac gaaaaacaat tacgggtcta ccatcgaggg 
     8941 cctgctcgat ctcccggacg acgacgcccc cgaagaggcg gggctggcgg ctccgcgcct 
     9001 gtcctttctc cccgcgggac acacgcgcag actgtcgacg gcccccccga ccgatgtcag 
     9061 cctgggggac gagctccact tagacggcga ggacgtggcg atggcgcatg ccgacgcgct 
     9121 agacgatttc gatctggaca tgttggggga cggggattcc ccgggtccgg gatttacccc 
     9181 ccacgactcc gccccctacg gcgctctgga tatggccgac ttcgagtttg agcagatgtt 
     9241 taccgatgcc cttggaattg acgagtacgg tgggtagggc cgaagccgct tggaataagg 
     9301 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag 
     9361 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc 
     9421 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg 
     9481 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag 
     9541 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca 
     9601 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt 
     9661 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc 
     9721 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa 
     9781 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatgaccgag tacaagccca 
     9841 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt 
     9901 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca 
     9961 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg 
    10021 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg 
    10081 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc 
    10141 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca 
    10201 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg 
    10261 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca 
    10321 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag 
    10381 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc 
    10441 agcgcccgac cgaaaggagc gcacgacccc atgcatcgat gatctagagc tcgctgatca 
    10501 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 
    10561 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 
    10621 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 
    10681 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 
    10741 gcggaaagaa cctgcagccc gggggatcca ctagttctag agcggccgcg gcgcgccgag 
    10801 ctcacgggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta 
    10861 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg 
    10921 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc 
    10981 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg gggatacggg gaaaaagctt 
    11041 ggccaaaaag gccgagctca cggggacagc ccccccccaa agcccccagg gatgtaatta 
    11101 cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc 
    11161 tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca 
    11221 cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctggggga 
    11281 tacggggaaa aagcttttaa ttaagcggcc gcactcgagt ttaccactcc ctatcagtga 
    11341 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc 
    11401 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat 
    11461 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg 
    11521 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact 
    11581 ccctatcagt gatagagaaa agtgaaagtc gagctcggta cccgggtcga ggtaggcgtg 
    11641 tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac 
    11701 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc 
    11761 ccgaattcgg taccggatcc agtcgagata tctagaaagg gcgaattcga cccaagtttg 
    11821 tacaaaaaag caggctttaa aggaaccaat tcagtcgact ggatccggta ccgaattcgc 
    11881 ggccgcactc gagatatcta gagtcgatcg acggatccgg ggaattcccc agtctcagga 
    11941 tccaccatgg ggcccaagaa gaaacgcaaa gtggggagca tgggggatcc cgtcgtttta 
    12001 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc 
    12061 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg 
    12121 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa 
    12181 agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag 
    12241 atgcacggtt acgatgcgcc catctacacc aacgtaacct atcccattac ggtcaatccg 
    12301 ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa 
    12361 agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat 
    12421 ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt 
    12481 gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgttgg 
    12541 agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac 
    12601 gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt 
    12661 aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt 
    12721 gactacctac gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc 
    12781 gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta 
    12841 cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg 
    12901 gtggttgaac tgcacaccgc cgacggcacg ctgattgaag cagaagcctg cgatgtcggt 
    12961 ttccgcgagg tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt 
    13021 cgaggcgtta accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg 
    13081 atggtgcagg atatcctgct gatgaagcag aacaacttta acgccgtgcg ctgttcgcat 
    13141 tatccgaacc atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtggat 
    13201 gaagccaata ttgaaaccca cggcatggtg ccaatgaatc tgctgaccga tgatccgcgc 
    13261 tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg 
    13321 agtgtgatca tctggtcgct ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg 
    13381 tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg cggcggagcc 
    13441 gacaccacgg ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc 
    13501 ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc 
    13561 ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa 
    13621 tactggcagg cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg ggactgggtg 
    13681 gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat 
    13741 tttggcgata cgccgaacca tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc 
    13801 acgccgcatc cagcgctgac ggaagcaaaa caccagcagc agtttttcca gttccgttta 
    13861 tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc 
    13921 ctgcactgga tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt gcctctggat 
    13981 gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc 
    14041 gggcaactct ggctcacagt acgcgtagtg caaccgaacg cgaccggatg gtcagaagcc 
    14101 gggcacatca gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc 
    14161 gccgcgtccc acgccatccc gcatctgacc accagcgaaa tggatttttg catcgagctg 
    14221 ggtaataagc gttggcaatt taaccgccag tcaggctttc tttcacagct gtggattggc 
    14281 gataaaaaac aactgctgac gccgctgcgc gatcagttca cccgtgcacc gctggataac 
    14341 gacattggcg taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag 
    14401 gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct 
    14461 gatgcggtgc tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc 
    14521 agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa 
    14581 gtggcgagcg atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta 
    14641 gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact 
    14701 gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc 
    14761 ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg 
    14821 cgcggcgact tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc 
    14881 catcgccatc tgctgcacgc ggaagaaggc acatggctga atatcgacgg tttccatatg 
    14941 gggattggtg gcgacgactc ctggagcccg tcagtatcgg cggaattaca gctgagcgcc 
    15001 ggtcgctacc attaccagtt ggtctggtgt caaaaataat aataaccggc aggccatgtc 
    15061 tgaaagtatt cgcgtaagga aatccattat gtactattta aaaaacacaa acttttggat 
    15121 gttcggttta ttctttttct tttacttttt tatcatggga gcctacttcc cgtttttccc 
    15181 gatttggcta catgacatca accatatgag caaaagtgat acgggtatta tttttgccgc 
    15241 tatttctctg ttgtcgctat tattccaacc gctgttggtc tgctttctga caaactcggc 
    15301 ctcgactcta gacccagctt t 
//
