LOCUS       Cre-ex-pRMCE-DV1-Hygro      6809 bp    DNA     circular SYN 7-JAN-2004
DEFINITION  LMBP 8175.
COMMENT     http://www.informaxinc.com/
FEATURES             Location/Qualifiers
     Misc._recombina join(6803..6809,1..14)
                     /label=attB1
     Misc._feature   41..1066
                     /label=Hygro
     Misc._recombina complement(1091..1111)
                     /label=attB2
     Intron          1116..1661
                     /vntifkey="15"
                     /label=IRES
     CDS             1663..2379
                     /vntifkey="4"
                     /label=EGFP
     Restriction_sit join(2701..2706,2701^2702)
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 19^20"
                     /note="cut_0_on_negative_strand: 23^24"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Misc._feature   2717..2959
                     /vntifkey="21"
                     /label=insulator
     Misc._feature   2960..3202
                     /vntifkey="21"
                     /label=insulator
     Misc._feature   3219..3739
                     /vntifkey="21"
                     /label="PGK promoter"
     Misc._feature   3747..3749
                     /vntifkey="21"
                     /label=ATG
     Misc._feature   3752..3799
                     /vntifkey="21"
                     /label="FRT mut5"
     Replication_ori 4064..4944
                     /vntifkey="33"
                     /label=ori
     Misc._feature   complement(5005..5865)
                     /vntifkey="21"
                     /label=AmpR
     Misc._feature   6483..6530
                     /vntifkey="21"
                     /label="FRT wt"
ORIGIN
        1 GTACAAAAAA GCAGCCGAAT TCGCCCTTGG CGTCGACACC ATGAAAAAGC CTGAACTCAC
       61 CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA
      121 GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATACGT
      181 CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT
      241 TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT
      301 GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA
      361 ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT
      421 TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG
      481 GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA
      541 CGACACCGTC AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA
      601 CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA
      661 CAATGGCCGC ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA
      721 CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT TGTATGGAGC AGCAGACGCG
      781 CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG CGGCTCCGGG CGTATATGCT
      841 CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC GGCAATTTCG ATGATGCAGC
      901 TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA GCCGGGACTG TCGGGCGTAC
      961 ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC TGTGTAGAAG TACTTGCCGA
     1021 TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG GAATAGCGCC GGCGGTGAAG
     1081 GGCGAATTCG CAGCTTTCTT GTACAAAGTG GTGATTCGAG GTCGACGGTA TCGATAAGCT
     1141 TGATATCGAA TTCCGCCCCT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT
     1201 GGAATAAGGC CGGTGAGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG
     1261 CAATGTGAGG GCCCGAAACC TGGCCCTGTC TTCTTGACGA CGATTCCTAG GGGTCTTTCC
     1321 CCTCTCGCCA AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT TCCTCTGGAA
     1381 GCTTCTTGAA GACAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC CCCCCACCTG
     1441 GCGACATGGA TAGTTGTGGA AAGAGTCAAA TGGCTCTCCT CAAGCGTATT CAACAAGGGG
     1501 CTGAAGGATG CCCAGAAGGT ACCCCATTGT ATGGGATCTG ATCTGGGGCC TCGGTGCACA
     1561 TGCTTTACAT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
     1621 TGGTTTTGGT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG CAAGGGCGAG
     1681 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC
     1741 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG
     1801 TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC
     1861 TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
     1921 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC
     1981 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
     2041 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC
     2101 AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC
     2161 AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
     2221 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC
     2281 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
     2341 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AAAGCGGCCG CGAGCTCGCT
     2401 GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC
     2461 CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG
     2521 CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA
     2581 AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT
     2641 CTGAGGCGGA AAGAACCAGC TGGGGCTCGA TCCTCTAGTT GGCGCGCCGG GCCCTCGCGA
     2701 CTCGAGGAAT AAATTCGAGC TCACGGGGAC AGCCCCCCCC CAAAGCCCCC AGGGATGTAA
     2761 TTACGTCCCT CCCCCGCTAG GGGGCAGCAG CGAGCCGCCC GGGGCTCCGC TCCGGTCCGG
     2821 CGCTCCCCCC GCATCCCCGA GCCGGCAGCG TGCGGGGACA GCCCGGGCAC GGGGAAGGTG
     2881 GCACGGGATC GCTTTCCTCT GAACGCTTCT CGCTGCTCTT TGAGCCTGCA GACACCTGGG
     2941 GGATACGGGG AAAAAGCTTG AGCTCACGGG GACAGCCCCC CCCCAAAGCC CCCAGGGATG
     3001 TAATTACGTC CCTCCCCCGC TAGGGGGCAG CAGCGAGCCG CCCGGGGCTC CGCTCCGGTC
     3061 CGGCGCTCCC CCCGCATCCC CGAGCCGGCA GCGTGCGGGG ACAGCCCGGG CACGGGGAAG
     3121 GTGGCACGGG ATCGCTTTCC TCTGAACGCT TCTCGCTGCT CTTTGAGCCT GCAGACACCT
     3181 GGGGGATACG GGGAAAAAGC TTGTCGACGA ATAAATTCAA TTCTACCGGG TAGGGGAGGC
     3241 GCTTTTCCCA AGGCAGTCTG GAGCATGCGC TTTAGCAGCC CCGCTGGGCA CTTGGCGCTA
     3301 CACAAGTGGC CTCTGGCCTC GCACACATTC CACATCCACC GGTAGGCGCC AACCGGCTCC
     3361 GTTCTTTGGT GGCCCCTTCG CGCCACCTTC TACTCCTCCC CTAGTCAGGA AGTTCCCCCC
     3421 CGCCCCGCAG CTCGCGTCGT GCAGGACGTG ACAAATGGAA GTAGCACGTC TCACTAGTCT
     3481 CGTGCAGATG GACAGCACCG CTGAGCAATG GAAGCGGGTA GGCCTTTGGG GCAGCGGCCA
     3541 ATAGCAGCTT TGCTCCTTCG CTTTCTGGGC TCAGAGGCTG GGAAGGGGTG GGTCCGGGGG
     3601 CGGGCTCAGG GGCGGGCTCA GGGGCGGGGC GGGCGCCCGA AGGTCCTCCG GAGGCCCGGC
     3661 ATTCTGCACG CTTCAAAAGC GCACGTCTGC CGCGCTGTTC TCCTCTTCCT CATCTCCGGG
     3721 CCTTTCGACC TGCAATCGCC GCCACCATGG AGAAGTTCCT ATTCCGAAGT TCCTATTCTT
     3781 CAAAAGGTAT AGGAACTTCC GATCGGGATC CCCAATCCTG CATGCAAGCT TGGCGTAATC
     3841 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG
     3901 AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT
     3961 TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG
     4021 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT
     4081 CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC
     4141 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG
     4201 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG
     4261 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     4321 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     4381 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     4441 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     4501 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     4561 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     4621 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     4681 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     4741 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     4801 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
     4861 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
     4921 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
     4981 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
     5041 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
     5101 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC
     5161 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
     5221 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
     5281 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
     5341 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
     5401 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
     5461 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
     5521 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
     5581 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
     5641 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
     5701 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
     5761 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
     5821 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
     5881 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
     5941 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT
     6001 CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT
     6061 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC
     6121 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC
     6181 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG
     6241 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG
     6301 GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC
     6361 GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC
     6421 AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTCCACA AAAGTGCGAT
     6481 CGGAAGTTCC TATTCCGAAG TTCCTATTCT CTAGAAAGTA TAGGAACTTC TTAATTAAGG
     6541 GATCTGTAGG GCGCAGTAGT CCAGGGTTTC CTTGATGATG TCATACTTAT CCTGTCCCTT
     6601 TTTTTTCCAC AGCTCGCGGT TGAGGACAAA CTCTTCGCGG TCTTTCCAGT GGGGATCGAC
     6661 GGTATCGTAG AGTCGAGGCC GCTCTAGAAC TAGTGGATCC GGAACCCTTA ATATAACTTC
     6721 GTATAATGTA TGCTATACGA AGTTATTAGG TCCCTCGACC TGCAGCCCAA GCTAGCTTAT
     6781 CGATACCGTC GACCTCGAAT CACAAGTTT
//
