LOCUS       Cre-excised_pCOIN_DV        6228 bp    DNA     circular UNA 15-OCT-2013
DEFINITION  LMBP 8191.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8191
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 15-OCT-2013
COMMENT     VNTDATE|675943638|
COMMENT     VNTNAME|Cre excised pCOIN DV|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "Cre excised pCOIN DV" 0 6228 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BamHI" "EcoRI" "NotI" "PvuII" "SacI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 2766 2777 1 6228 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "PvuII" "cagctg" 4 0 4 1328 0 3692 0 5520 0 
COMMENT            6206 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "SacI" "gagctc" 6 0 3 40 0 283 0 5943 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 5737 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 4 1125 0 4014 0 4963 0 
COMMENT            5666 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 3780 0 5416 0 0 "")) 
COMMENT       (CObList 
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COMMENT            "chicken \337-globin 5'HS4 insulator core sequence" 21 0 0 35 277 0 
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COMMENT            "chicken \337-globin 5'HS4 insulator core sequence" 21 0 0 278 520 0 
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COMMENT        #7=(CFSignal (CObList) "FRT mutant" 21 0 0 1070 1117 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1070..1117") 
COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2323 3183 0 
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COMMENT        #9=(CFSignal (CObList) "FRT" 21 0 0 3801 3848 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3801..3848") 
COMMENT        #10=(CFSignal (CObList) "loxP" 21 0 1 4031 4064 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "attR4" 0 0 1 4162 4286 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4162..4286)") 
COMMENT        #12=(CFSignal (CObList) "ccdB" 4 0 1 4327 4632 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4327..4632)") 
COMMENT        #13=(CFSignal (CObList) "chloramphenicol resistance" 4 0 1 4974 5633 0 
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COMMENT        #14=(CFSignal (CObList) "attR3" 0 0 0 5743 5867 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5741..5865") 
COMMENT        #15=(CFSignal (CObList) "mPGK1 promoter" 29 0 0 539 1046 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "539..1046") 
COMMENT        #16=(CFSignal (CObList) "lac promoter" 30 0 1 1205 1288 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "pMB1 ori" 33 0 1 1380 1782 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1380..1782)") 
COMMENT        #18=(CFSignal (CObList) "BGH polyA" 43 0 0 5959 6183 0 (CStringList) 
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COMMENT        #19=(CFSignal (CObList) "lacUV5 promoter" 30 0 1 5682 5735 0 
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COMMENT        #20=(CFSignal (CObList) "SA" 21 0 0 3865 3968 0 (CStringList) 
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COMMENT       (CObList 
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COMMENT        #22=(CFSignal (CObList) "Last modified: 15-OCT-2013" 2 0 0 0 0 0 
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COMMENT                          "Created: 10/15/13 10:07" 1) 
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COMMENT                          "Last Modified: 10/15/13 10:07" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6228 bp" 1) 
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COMMENT                          "Division: UNA" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 8-JUL-2013" 1))) 
COMMENT                   "Standard Fields") 
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FEATURES             Location/Qualifiers
     misc_feature    35..277
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    278..520
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    1070..1117
                     /vntifkey="21"
                     /label=FRT\mutant
     CDS             complement(2323..3183)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    3801..3848
                     /vntifkey="21"
                     /label=FRT
     misc_feature    complement(4031..4064)
                     /vntifkey="21"
                     /label=loxP
     attenuator      complement(4162..4286)
                     /vntifkey="0"
                     /label=attR4
     CDS             complement(4327..4632)
                     /vntifkey="4"
                     /label=ccdB
     CDS             complement(4974..5633)
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     attenuator      5743..5867
                     /vntifkey="0"
                     /label=attR3
     promoter        539..1046
                     /vntifkey="29"
                     /label=mPGK1\promoter
     promoter        complement(1205..1288)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(1380..1782)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      5959..6183
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        complement(5682..5735)
                     /vntifkey="30"
                     /label=lacUV5\promoter
     misc_feature    3865..3968
                     /vntifkey="21"
                     /label=SA
BASE COUNT     1489 a      1626 c      1569 g      1542 t         2 others
ORIGIN
        1 cgcgccgggc cctcgcgact cgaggaataa attcgagctc acggggacag ccccccccca 
       61 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg 
      121 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc 
      181 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg 
      241 agcctgcaga cacctggggg atacggggaa aaagcttgag ctcacgggga cagccccccc 
      301 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc 
      361 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac 
      421 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct 
      481 ttgagcctgc agacacctgg gggatacggg gaaaaagctt gtcgacgaat aaattcaatt 
      541 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 
      601 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 
      661 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 
      721 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 
      781 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 
      841 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 
      901 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 
      961 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 
     1021 ctcttcctca tctccgggcc tttcgacctg caatcgccgc caccatggag aagttcctat 
     1081 tccgaagttc ctattcttca aaaggtatag gaacttccga tcgggatccc caatcctgca 
     1141 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     1201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     1261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     1321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     1381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     1441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     1501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     1561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     1621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     1681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     1741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     1801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     1861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     1921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     1981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 
     2041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     2101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     2161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     2221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     2281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     2341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     2401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     2461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     2521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     2581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
     2641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     2701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     2761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     2821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
     2881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
     2941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
     3001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
     3061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
     3121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
     3181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
     3241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
     3301 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 
     3361 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 
     3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 
     3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 
     3541 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 
     3601 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 
     3661 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 
     3721 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 
     3781 attccacaaa agtgcgatcg gaagttccta ttccgaagtt cctattctct agaaagtata 
     3841 ggaacttctt aattaaggga tctgtagggc gcagtagtcc agggtttcct tgatgatgtc 
     3901 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc 
     3961 tttccagtgg ggatcgacgg tatcgtagag tcgaggccgc tctagaacta gtggatccgg 
     4021 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgacctg 
     4081 cagcccaagc tagcttatcg ataccgtcga cnctcgagnt tttcccagtc acgacgttgt 
     4141 aaaacgacgg ccagtgaatt atcaactttg tatagaaaag ttgaacgaga aacgtaaaat 
     4201 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt 
     4261 aaaacacaac atatccagtc actatggtcg acctgcagac tggctgtgta taagggagcc 
     4321 tgacatttat attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg 
     4381 gctgagatca gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt 
     4441 catcatgcgc cagctttcat ccccgatatg caccaccggg taaagttcac gggggacttt 
     4501 atctgacagc agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat 
     4561 aatatcactc tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac 
     4621 cttaaactgc atttcaccag cccctgttct cgtcggcaaa agagccgttc atttcaataa 
     4681 accgggcgac ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac 
     4741 gacgggcttc attctgcatg gttgtgctta ccgaaccgga gatattgaca tcatatatgc 
     4801 cttgagcaac tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcata 
     4861 cctctttttg acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca 
     4921 gcgcgcaaat acgcatactg ttatctggct tttagtaagc cggatcctct agattacgcc 
     4981 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag 
     5041 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc 
     5101 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt 
     5161 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata 
     5221 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg 
     5281 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt 
     5341 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct 
     5401 ttcattgcca tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag 
     5461 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc 
     5521 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta 
     5581 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct 
     5641 tccttagctc ctgaaaatct cgacggatcc taactcaaaa tccacacatt atacgagccg 
     5701 gaagcataaa gtgtaaagcc tgggggtgcc taatgcggcc gccatagtga ctggatatgt 
     5761 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata 
     5821 tttatatcat tttacgtttc tcgttcaact ttattataca tagttgatag cttgatagct 
     5881 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc gggccgcgag 
     5941 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 
     6001 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 
     6061 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 
     6121 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 
     6181 tggcttctga ggcggaaaga accagctggg gctcgatcct ctagttgg 
//
