LOCUS Cre-excised_pRMCE_DV1 7394 bp DNA circular UNA 4-NOV-2013 DEFINITION LMBP 8195. SOURCE ORGANISM COMMENT Accession number: LMBP 8195 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) Last modified: 4-NOV-2013 COMMENT VNTDATE|677782603| COMMENT VNTNAME|Cre excised pRMCE DV1| COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "Cre excised pRMCE DV1" 0 7394 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 COMMENT 0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) COMMENT (CWordArray) (CStringList "EcoRI" "EcoRV" "PvuII" "XmnI") COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 COMMENT "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 1 7107 7106 1 7394 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList COMMENT #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 3780 0 4567 0 5861 0 0 COMMENT "") #1=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 5857 0 0 "") COMMENT #2=(CRSite (CStringList) "PvuII" "cagctg" 4 0 4 1328 0 3692 0 4467 0 COMMENT 7372 0 0 "") COMMENT #3=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 4 30 0 532 0 2996 0 COMMENT 6080 0 0 "")) COMMENT (CObList COMMENT #4=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2323 3183 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2323..3183)") COMMENT #5=(CFSignal (CObList) "loxP" 21 0 1 4031 4064 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(4031..4064)") COMMENT #6=(CFSignal (CObList) "attR1" 0 0 1 4120 4244 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(4120..4244)") COMMENT #7=(CFSignal (CObList) "attR2" 0 0 0 5700 5824 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5700..5824") COMMENT #8=(CFSignal (CObList) "5' UTR of the EMCV polyprotein gene (IRES)" 32 0 COMMENT 0 5868 6362 0 (CStringList) (CStringList) 1 1 1 1 "5868..6362") COMMENT #9=(CFSignal (CObList) "EGFP" 4 0 0 6375 7094 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "6375..7094") COMMENT #10=(CFSignal (CObList) "lac promoter" 30 0 1 1205 1288 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(1205..1288)") COMMENT #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 1380 1782 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(1380..1782)") COMMENT #12=(CFSignal (CObList) "mPGK1 promoter" 29 0 0 539 1046 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "539..1046") COMMENT #13=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 4252 4304 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "4252..4304") COMMENT #14=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 4353 5012 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "4353..5012") COMMENT #15=(CFSignal (CObList) "ccdB" 4 0 0 5354 5659 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5354..5659") COMMENT #16=(CFSignal (CObList) "BGH polyA" 43 0 0 7125 7349 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "7125..7349") COMMENT #17=(CFSignal (CObList) COMMENT "chicken \337-globin 5'HS4 insulator core sequence" 21 0 0 35 277 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "35..277") COMMENT #18=(CFSignal (CObList) COMMENT "chicken \337-globin 5'HS4 insulator core sequence" 21 0 0 278 520 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "278..520") COMMENT #19=(CFSignal (CObList) "FRT mutant" 21 0 0 1070 1117 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1070..1117") COMMENT #20=(CFSignal (CObList) "FRT" 21 0 0 3801 3848 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "3801..3848")) (CObList) (CObList) (CObList) COMMENT (CObList) COMMENT (CObList COMMENT #21=(CFSignal (CObList) "LMBP 8195" 2 0 0 0 0 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "") COMMENT #22=(CFSignal (CObList) "Last modified: 4-NOV-2013" 2 0 0 0 0 0 COMMENT (CStringList) (CStringList) 1 1 0 0 "")) (CObList) COMMENT (CTextView 0 COMMENT #23=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #24=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) COMMENT "Cre excised pRMCE DV1" 2) 5 "" 0 4) COMMENT #25=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'Cre excised pRMCE DV1'" 1) COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Created: 11/04/13 04:56" 1) COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modified: 11/04/13 05:06" 1) COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 7394 bp" 1) COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #33=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Original Source Database: GenBank" 1) COMMENT #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Division: UNA" 1) COMMENT #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Modification Date in the Original DB: 8-JUL-2013" 1))) COMMENT "Standard Fields") COMMENT #37=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #38=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) COMMENT (CObjectList COMMENT #39=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) COMMENT (CObjectList COMMENT #40=(CLinePar (CParagraph 0 (15 #21# 0) 1 2 2 0 180) COMMENT "" 1))) "LMBP 8195") COMMENT #41=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) COMMENT (CObjectList COMMENT #42=(CLinePar (CParagraph 0 (15 #22# 0) 1 2 2 0 180) COMMENT "" 1))) "Last modified: 4-NOV-2013"))) COMMENT "Annotations") COMMENT #43=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 2 0 0 178) COMMENT (CObjectList COMMENT #44=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (7 0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #45=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4120 (3 #6# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #46=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4120 End: 4244 (Complementary)" 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pRMCE DV1" 3084 2996 "XmnI" 6080 "XmnI" COMMENT (LOGPEN 0 0 0) 1.37517e-316 5.25394e-283) COMMENT #288=(CSampFrag "Cre ex. pRMCE DV1" 2464 532 "XmnI" 2996 "XmnI" COMMENT (LOGPEN 0 0 0) 1.97626e-323 5.24435e-283) COMMENT #289=(CSampFrag "Cre ex. pRMCE DV1" 1344 6080 "XmnI" 30 "XmnI" COMMENT (LOGPEN 0 0 0) 1.40047e-316 1.02894e-314) COMMENT #290=(CSampFrag "Cre ex. pRMCE DV1" 502 30 "XmnI" 532 "XmnI" COMMENT (LOGPEN 0 0 0) 1.43911e-316 9.5261e-308)))) FEATURES Location/Qualifiers misc_feature 35..277 /vntifkey="21" /label=chicken\ß-globin\5'HS4\insulator\core\sequence misc_feature 278..520 /vntifkey="21" /label=chicken\ß-globin\5'HS4\insulator\core\sequence misc_feature 1070..1117 /vntifkey="21" /label=FRT\mutant CDS complement(2323..3183) /vntifkey="4" /label=ampicillin\resistance misc_feature 3801..3848 /vntifkey="21" /label=FRT misc_feature complement(4031..4064) /vntifkey="21" /label=loxP attenuator complement(4120..4244) /vntifkey="0" /label=attR1 attenuator 5700..5824 /vntifkey="0" /label=attR2 RBS 5868..6362 /vntifkey="32" /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES) CDS 6375..7094 /vntifkey="4" /label=EGFP promoter complement(1205..1288) /vntifkey="30" /label=lac\promoter rep_origin complement(1380..1782) /vntifkey="33" /label=pMB1\ori promoter 539..1046 /vntifkey="29" /label=mPGK1\promoter promoter 4252..4304 /vntifkey="30" /label=lacUV5\promoter CDS 4353..5012 /vntifkey="4" /label=chloramphenicol\resistance CDS 5354..5659 /vntifkey="4" /label=ccdB terminator 7125..7349 /vntifkey="43" /label=BGH\polyA BASE COUNT 1770 a 1941 c 1944 g 1739 t ORIGIN 1 cgcgccgggc cctcgcgact cgaggaataa attcgagctc acggggacag ccccccccca 61 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg 121 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc 181 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg 241 agcctgcaga cacctggggg atacggggaa aaagcttgag ctcacgggga cagccccccc 301 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc 361 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac 421 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct 481 ttgagcctgc agacacctgg gggatacggg gaaaaagctt gtcgacgaat aaattcaatt 541 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 601 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 661 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 721 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 781 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 841 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 901 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 961 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 1021 ctcttcctca tctccgggcc tttcgacctg caatcgccgc caccatggag aagttcctat 1081 tccgaagttc ctattcttca aaaggtatag gaacttccga tcgggatccc caatcctgca 1141 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 1201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 1261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 1321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 1381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 1441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 1501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 1561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 1621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 1681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 1741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 1801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 1861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 1921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 1981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 2041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 2101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 2161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 2221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 2281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 2341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 2401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 2461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 2521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 2581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 2641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 2701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 2761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 2821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 2881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 2941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 3001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 3061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 3121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 3181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 3241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 3301 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 3361 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 3541 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 3601 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 3661 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 3721 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 3781 attccacaaa agtgcgatcg gaagttccta ttccgaagtt cctattctct agaaagtata 3841 ggaacttctt aattaaggga tctgtagggc gcagtagtcc agggtttcct tgatgatgtc 3901 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc 3961 tttccagtgg ggatcgacgg tatcgtagag tcgaggccgc tctagaacta gtggatccgg 4021 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgacctg 4081 cagcccaagc tagcttatcg ataccgtcga cctcgaatca caagtttgta caaaaaagct 4141 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa 4201 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca 4261 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc 4321 cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca 4381 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc 4441 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga 4501 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc 4561 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc 4621 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc 4681 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa 4741 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct 4801 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg 4861 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc 4921 aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac 4981 agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca 5041 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt 5101 atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga 5161 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc 5221 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag 5281 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac 5341 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct 5401 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct 5461 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat 5521 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat 5581 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct 5641 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc 5701 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct 5761 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag 5821 tggtgattcg aggtcgacgg tatcgataag cttgatatcg aattccgccc ctctccctcc 5881 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgagc gtttgtctat 5941 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccgaaa cctggccctg 6001 tcttcttgac gacgattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt 6061 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaacaa cgtctgtagc 6121 gaccctttgc aggcagcgga accccccacc tggcgacatg gatagttgtg gaaagagtca 6181 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt 6241 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa 6301 aaaacgtcta ggccccccga accacgggga cgtggttttg gtttgaaaaa cacgatgata 6361 atatggccac aaccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 6421 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 6481 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 6541 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc 6601 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 6661 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 6721 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 6781 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 6841 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 6901 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 6961 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 7021 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 7081 agctgtacaa gtaaagcggc cgcgagctcg ctgatcagcc tcgactgtgc cttctagttg 7141 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 7201 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 7261 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 7321 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 7381 gatcctctag ttgg //