LOCUS       pENTR1A-Neoprobe        3312 bp    DNA     circular UNA  1-AUG-2014
DEFINITION  LMBP 8197.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     CDS             36..830
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     attenuator      complement(1062..1157)
                     /vntifkey="0"
                     /label=attL2
     CDS             1281..2090
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
     rep_origin      2550..2875
                     /vntifkey="33"
                     /label=pMB1\ori
     attenuator      3189..3284
                     /vntifkey="0"
                     /label=attL1
     terminator      complement(2936..2979)
                     /vntifkey="43"
                     /label=T1\terminator
     terminator      3111..3138
                     /vntifkey="43"
                     /label=T2\terminator
BASE COUNT      780 a       805 c       875 g       852 t 
ORIGIN
        1 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 
       61 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 
      121 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 
      181 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt 
      241 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 
      301 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 
      361 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 
      421 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 
      481 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 
      541 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg 
      601 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 
      661 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 
      721 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 
      781 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct 
      841 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac 
      901 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 
      961 cctccagcgc ggggatctca tgctggagtt cttcgccgtt tccctgccac agtctgagag 
     1021 ctccctggcg aattcgcggc cgcactcgag atatctagac ccagctttct tgtacaaagt 
     1081 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca 
     1141 aaataaaatc attatttgcc atccagctgc agctctggcc cgtgtctcaa aatctctgat 
     1201 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa 
     1261 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa 
     1321 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat 
     1381 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac 
     1441 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga 
     1501 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt 
     1561 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt 
     1621 caggtgaaaa tattgttgat gcgctggcag tgtccctgcg ccggttgcat tcgattcctg 
     1681 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa 
     1741 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg 
     1801 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc 
     1861 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg 
     1921 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc 
     1981 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc 
     2041 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg 
     2101 ttaattggtt gtaacattat tcagattggg ccccgttcca ctgagcgtca gacccggtag 
     2161 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     2221 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     2281 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 
     2341 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     2401 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     2461 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     2521 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     2581 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     2641 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     2701 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     2761 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     2821 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca 
     2881 tggatctcgg ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa 
     2941 acgaaaggct cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 
     3001 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg 
     3061 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat 
     3121 cctgacggat ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct 
     3181 cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga 
     3241 taagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa 
     3301 ttcagtcgac tg 
//
