LOCUS       pEntry-attR2-IRES-eGFP-luc+-pA-attL3        6273 bp    DNA     circular     30-JUL-2013
DEFINITION  LMBP 8200.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8200
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 30-JUL-2013

COMMENT     VNTDATE|545501441|
COMMENT     VNTNAME|pEntry attR2-IRES-eGFP-luc+-pA-attL3|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT            5009 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HincII" "gtyrac" 4 0 4 129 0 2407 0 2679 0 
COMMENT            5364 0 0 "") 
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COMMENT            "298..1107") 
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COMMENT            (CStringList) 1 1 1 1 "complement(13..108)") 
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COMMENT            (CStringList) 1 1 1 1 "4055..5704") 
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FEATURES             Location/Qualifiers
     terminator      complement(2173..2200)
                     /vntifkey="43"
                     /label=T2\terminator
     terminator      2332..2375
                     /vntifkey="43"
                     /label=T1\terminator
     promoter        complement(149..165)
                     /vntifkey="30"
                     /label=T7\promoter
     CDS             299..1108
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
     attenuator      complement(14..109)
                     /vntifkey="0"
                     /label=attL3
     CDS             4062..5711
                     /vntifkey="4"
                     /label=LUCm
     CDS             3321..4037
                     /vntifkey="4"
                     /label=EGFP
     rep_origin      1625..2027
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      5727..5818
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_feature    5819..6175
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     attenuator      complement(2529..2653)
                     /vntifkey="0"
                     /label=attR2
     RBS             2711..3291
                     /vntifkey="32"
                     /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES)
BASE COUNT     1597 a      1542 c      1536 g      1598 t 
ORIGIN
        1 tcgagatatc tagcaacttt attatacaaa gttggcatta taaaaaagca ttgcttatca 
       61 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg gagctccatg 
      121 gtagcgttaa cgcggccgcg atatccccta tagtgagtcg tattacatgg tcatagctgt 
      181 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa 
      241 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat 
      301 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt 
      361 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt 
      421 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa 
      481 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac 
      541 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg 
      601 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc 
      661 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag 
      721 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc 
      781 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca 
      841 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa 
      901 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc 
      961 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt 
     1021 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt 
     1081 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca 
     1141 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag 
     1201 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 
     1261 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 
     1321 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 
     1381 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 
     1441 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 
     1501 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 
     1561 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 
     1621 cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa 
     1681 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 
     1741 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 
     1801 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 
     1861 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 
     1921 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 
     1981 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 
     2041 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 
     2101 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt 
     2161 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca 
     2221 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc 
     2281 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga 
     2341 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct 
     2401 cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct 
     2461 taagctcggg ccctgcagct ctagagctcg aattctacag gtcactaata ccatctaagt 
     2521 agttgattca tagtgactgc atatgttgtg ttttacagta ttatgtagtc tgttttttat 
     2581 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcaactttct 
     2641 tgtacaaagt ggttaaagga accaattcag tcgaggtcga cggtatcgat aagcttgata 
     2701 tcgaattccg cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct 
     2761 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg 
     2821 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt 
     2881 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg 
     2941 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac 
     3001 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg 
     3061 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct 
     3121 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg 
     3181 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag 
     3241 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca 
     3301 accatggtat ggccacaacc atggtgagca agggcgagga gctgttcacc ggggtggtgc 
     3361 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 
     3421 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 
     3481 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc 
     3541 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 
     3601 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 
     3661 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 
     3721 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 
     3781 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 
     3841 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 
     3901 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 
     3961 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 
     4021 tggacgagct gtacaagtac tcagatccac cggtcgccac cgaagacgcc aaaaacataa 
     4081 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata 
     4141 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg 
     4201 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga 
     4261 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat 
     4321 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca 
     4381 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg 
     4441 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa 
     4501 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg 
     4561 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata 
     4621 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg 
     4681 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg 
     4741 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg 
     4801 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat 
     4861 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg 
     4921 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta 
     4981 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg 
     5041 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag 
     5101 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat 
     5161 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag 
     5221 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga 
     5281 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg 
     5341 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc 
     5401 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag 
     5461 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg 
     5521 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa 
     5581 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg 
     5641 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga 
     5701 tcgccgtgta attctagagc ggccgcgatc tttttccctc tgccaaaaat tatggggaca 
     5761 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa 
     5821 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt 
     5881 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg 
     5941 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc 
     6001 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg 
     6061 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt 
     6121 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg 
     6181 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaagggcgaa 
     6241 ttctgcagat atccatcaca ctggcggccg cac 
//
