LOCUS       pUgly-VEGF.2113      5010 bp    DNA     circular UNA 19-OCT-2007
DEFINITION  LMBP 8203.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Misc._feature   67..166
                     /vntifkey="21"
                     /label=attL1
     Misc._feature   197..629
                     /vntifkey="21"
                     /label=TRE
     Misc._feature   659..1375
                     /vntifkey="21"
                     /label=eGFP
     Misc._feature   1398..1518
                     /vntifkey="21"
                     /label="mir30 5'"
                     /label="mir30 5'"
     Misc._feature   1523..1625
                     /vntifkey="21"
                     /label=VEGFA.2113
                     /label=VEGF.2113
     Misc._feature   1626..1740
                     /vntifkey="21"
                     /label="mir30 3'"
                     /label="mir30 3'"
     Misc._feature   complement(1780..1879)
                     /vntifkey="21"
                     /label=attL2
     Misc._feature   2662..3196
                     /vntifkey="21"
                     /label=Gentamicin
     Replication_ori 4043..4717
                     /vntifkey="33"
                     /label=Ori
ORIGIN
        1 CTGACGGATG GCCTTTTTGC GTTTCTACAA ACTCTTCCTG TTAGTTAGTT ACTTAAGCTC
       61 GGGCCCCAAA TAATGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT
      121 AAGCAATGCT TTTTTATAAT GCCAACTTTG TACAAAAAAG CAGGCTTTAA AGGAACCAAT
      181 TCAGTCGACT GGATCCTTTA CCACTCCCTA TCAGTGATAG AGAAAAGTGA AAGTCGAGTT
      241 TACCACTCCC TATCAGTGAT AGAGAAAAGT GAAAGTCGAG TTTACCACTC CCTATCAGTG
      301 ATAGAGAAAA GTGAAAGTCG AGTTTACCAC TCCCTATCAG TGATAGAGAA AAGTGAAAGT
      361 CGAGTTTACC ACTCCCTATC AGTGATAGAG AAAAGTGAAA GTCGAGTTTA CCACTCCCTA
      421 TCAGTGATAG AGAAAAGTGA AAGTCGAGTT TACCACTCCC TATCAGTGAT AGAGAAAAGT
      481 GAAAGTCGAG CTCGGTACCC GGGTCGAGGT AGGCGTGTAC GGTGGGAGGC CTATATAAGC
      541 AGAGCTCGTT TAGTGAACCG TCAGATCGCC TGGAGACGCC ATCCACGCTG TTTTGACCTC
      601 CATAGAAGAC ACCGGGACCG ATCCAGCCTC CGCGGCCCCG AACTAGGCCT AGTCCACCAT
      661 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
      721 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
      781 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
      841 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA
      901 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
      961 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
     1021 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA
     1081 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG
     1141 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA
     1201 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
     1261 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT
     1321 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAAC
     1381 TAGTGACTCT AGTCCTAGTC GACTAGGGAT AACAGGGTAA TTGTTTGAAT GAGGCTTCAG
     1441 TACTTTACAG AATCGTTGCC TGCACATCTT GGAAACACTT GCTGGGATTA CTTCTTCAGG
     1501 TTAACCCAAC AGAAGGCTCG AGAAGGTATA TGCTGTTGAC AGTGAGCGAG GTACTTATTT
     1561 AATAGCCCTT TAGTGAAGCC ACAGATGTAA AGGGCTATTA AATAAGTACC GTGCCTACTG
     1621 CCTCGGAATT CAAGGGGCTA CTTTAGGAGC AATTATCTTG TTTACTAAAA CTGAATACCT
     1681 TGCTATCTCT TTGATACATT TTTACAAAGC TGAATTAAAA TGGTATAAAT TAAATCACTT
     1741 TTTTCAATTG GAAGACTAAT GCGTTTTCGA GATATCTAGA CCCAGCTTTC TTGTACAAAG
     1801 TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC
     1861 AAAATAAAAT CATTATTTGC CATCCAGCTG CAGCTCTGGC CCGTGTCTCA AAATCTCTGA
     1921 TGTTACATTG CACAAGATAA AAATATATCA TCATGAACAA TAAAACTGTC TGCTTACATA
     1981 AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG GCCGCGATTA
     2041 AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA
     2101 TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA
     2161 CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG
     2221 ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG
     2281 TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT
     2341 TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTCCCTGC GCCGGTTGCA TTCGATTCCT
     2401 GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA
     2461 ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT
     2521 GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC AGTCGTCACT
     2581 CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAGG CCGGGAAGCC
     2641 GATCTCGGCT TGAACGAATT GTTAGGTGGC GGTACTTGGG TCGATATCAA AGTGCATCAC
     2701 TTCTTCCCGT ATGCCCAACT TTGTATAGAG AGCCACTGCG GGATCGTCAC CGTAATCTGC
     2761 TTGCACGTAG ATCACATAAG CACCAAGCGC GTTGGCCTCA TGCTTGAGGA GATTGATGAG
     2821 CGCGGTGGCA ATGCCCTGCC TCCGGTGCTC GCCGGAGACT GCGAGATCAT AGATATAGAT
     2881 CTCACTACGC GGCTGCTCAA ACCTGGGCAG AACGTAAGCC GCGAGAGCGC CAACAACCGC
     2941 TTCTTGGTCG AAGGCAGCAA GCGCGATGAA TGTCTTACTA CGGAGCAAGT TCCCGAGGTA
     3001 ATCGGAGTCC GGCTGATGTT GGGAGTAGGT GGCTACGTCT CCGAACTCAC GACCGAAAAG
     3061 ATCAAGAGCA GCCCGCATGG ATTTGACTTG GTCAGGGCCG AGCCTACATG TGCGAATGAT
     3121 GCCCATACTT GAGCCACCTA ACTTTGTTTT AGGGCGACTG CCCTGCTGCG TAACATCGTT
     3181 GCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCAAACA TCGACCCACG GCGTAACGCG
     3241 CTTGCTGCTT GGATGCCCGA GGCATAGACT GTACAAAAAA ACAGTCATAA CAAGCCATGA
     3301 AAACCGCCAC TGCGCCGTTA CCACCGCTGC GTTCGGTCAA GGTTCTGGAC CAGTTGCGTG
     3361 AGCGCATACG CTACTTGCAT TACAGTTTAC GAACCGAACA GGCTTATGTC AACTGGGTTC
     3421 GTGCCTTCAT CCGTTTCCAC GGTGTGCGTC ACCCGGCAAC CTTGGGCAGC AGCGAAGTCG
     3481 AGGCATTTCT GTCCTGGCTG GCGAACGAGC GCAAGGTTTC GGTCTCCACG CATCGTCAGG
     3541 CATTGGCGGC CTTGCTGTTC TTCTACGGCA AGGTGCTGTG CACGGATCTG CCCTGGCTTC
     3601 AGGAGATCGG AAGACCTCGG CCGTCGCGGC GCTTGCCGGT GGTGCTGACC CCGGATGAAG
     3661 TGGTTCGCAT CCTCGGTTTT CTGGAAGGCG AGCATCGTTT GTTCGCCCAG GACTCTAGCT
     3721 ATAGTTCTAG TGGTTGGCTA CTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG
     3781 ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC
     3841 AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC
     3901 ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA
     3961 GCATTACGCT GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT AACGTGAGTT
     4021 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT
     4081 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG
     4141 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA
     4201 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT
     4261 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA
     4321 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC
     4381 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT
     4441 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA
     4501 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG
     4561 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT
     4621 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT
     4681 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA
     4741 TTCTGTGGAT AACCGTATTA CCGCTAGCAT GGATCTCGGG GACGTCTAAC TACTAAGCGA
     4801 GAGTAGGGAA CTGCCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGGAAGA CTGGGCCTTT
     4861 CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC GCCGGGAGCG
     4921 GATTTGAACG TTGTGAAGCA ACGGCCCGGA GGGTGGCGGG CAGGACGCCC GCCATAAACT
     4981 GCCAGGCATC AAACTAAGCA GAAGGCCATC
//
