LOCUS       pEntry-L4-rtTA-IRES-Puro-pA-TRE-R1      5466 bp    DNA     circular UNA 28-JUL-2021
DEFINITION  LMBP 8214.
COMMENT     Accession number: LMBP 8214
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-JUL-2021
FEATURES             Location/Qualifiers
     CDS             join(4870..5466,1..3)
                     /label=puromycin\resistance
     terminator      90..314
                     /label=BGH\polyA
     promoter        374..742
                     /label=hCMVT\promoter
     misc_recomb     complement(872..980)
                     /label=attR1
     CDS             1090..1899
                     /label=Tn903\neomycin\resistance
     rep_origin      2359..2761
                     /label=pMB1\ori
     terminator      complement(2840..2883)
                     /label=T1\terminator
     terminator      3015..3042
                     /label=T2\terminator
     misc_recomb     3116..3211
                     /label=attL4
     CDS             3309..4313
                     /label=rtTA
     RBS             4314..4857
                     /label=IRES\of\EMCV\polyprotein\gene
ORIGIN
        1 TGACGCCCGC CCCACGACCC GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG
       61 ATGATCTAGA GCTCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT
      121 TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA
      181 ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG
      241 GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC
      301 GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCTGCAGC CCGGGGGATC CACTAGTTCT
      361 AGAGCGGCCG CACTCGAGTT TACCACTCCC TATCAGTGAT AGAGAAAAGT GAAAGTCGAG
      421 TTTACCACTC CCTATCAGTG ATAGAGAAAA GTGAAAGTCG AGTTTACCAC TCCCTATCAG
      481 TGATAGAGAA AAGTGAAAGT CGAGTTTACC ACTCCCTATC AGTGATAGAG AAAAGTGAAA
      541 GTCGAGTTTA CCACTCCCTA TCAGTGATAG AGAAAAGTGA AAGTCGAGTT TACCACTCCC
      601 TATCAGTGAT AGAGAAAAGT GAAAGTCGAG TTTACCACTC CCTATCAGTG ATAGAGAAAA
      661 GTGAAAGTCG AGCTCGGTAC CCGGGTCGAG GTAGGCGTGT ACGGTGGGAG GCCTATATAA
      721 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC
      781 TCCATAGAAG ACACCGGGAC CGATCCAGCC TCCGCGGCCC CGAATTCGGT ACCGGATCCA
      841 GTCGAGATAT CTAGAAAGGG CGAATTCGAC CCAAGTTTGT ACAAAAAAGT TGAACGAGAA
      901 ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA
      961 TAATACTGTA AAACACAACA GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA
     1021 CAAGATAAAA ATATATCATC ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA
     1081 AGGGGTGTTA TGAGCCATAT TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG
     1141 GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA
     1201 ATCTATCGCT TGTATGGGAA GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT
     1261 AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG
     1321 CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT
     1381 GCGATCCCCG GAAAAACAGC ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT
     1441 ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT
     1501 CCTTTTAACA GCGATCGCGT ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT
     1561 TTGGTTGATG CGAGTGATTT TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG
     1621 AAAGAAATGC ATAAACTTTT GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC
     1681 TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA
     1741 GTCGGAATCG CAGACCGATA CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT
     1801 TCTCCTTCAT TACAGAAACG GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT
     1861 AAATTGCAGT TTCATTTGAT GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG
     1921 TAACATTATT CAGATTGGGC CCCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA
     1981 GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA
     2041 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA
     2101 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC
     2161 CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA
     2221 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA
     2281 CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG
     2341 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT
     2401 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC
     2461 ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC
     2521 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC
     2581 GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC
     2641 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT
     2701 ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG
     2761 CGCCCAATAC GCAAACCGCT AGCATGGATC TCGGGGACGT CTAACTACTA AGCGAGAGTA
     2821 GGGAACTGCC AGGCATCAAA TAAAACGAAA GGCTCAGTCG GAAGACTGGG CCTTTCGTTT
     2881 TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGG GAGCGGATTT
     2941 GAACGTTGTG AAGCAACGGC CCGGAGGGTG GCGGGCAGGA CGCCCGCCAT AAACTGCCAG
     3001 GCATCAAACT AAGCAGAAGG CCATCCTGAC GGATGGCCTT TTTGCGTTTC TACAAACTCT
     3061 TCCTGTTAGT TAGTTACTTA AGCTCGGGCC CGCGTTAACG CTACCATGGA GCTCCAAATA
     3121 ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATAA GCAATGCTTT
     3181 TTTATAATGC CAACTTTGTA TAGAAAAGTT GGCTCCGAAT TCGCCCTTTT AAAGGAACCA
     3241 ATTCAGTCGA GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCACCATG CCAAAGAGAC
     3301 CCAGACCCTC TAGATTAGAT AAAAGTAAAG TGATTAACAG CGCATTAGAG CTGCTTAATG
     3361 AGGTCGGAAT CGAAGGTTTA ACAACCCGTA AACTCGCCCA GAAGCTTGGT GTAGAGCAGC
     3421 CTACACTGTA TTGGCATGTA AAAAATAAGC GGGCTTTGCT CGACGCCTTA GCCATTGAGA
     3481 TGTTAGATAG GCACCATACT CACTTTTGCC CTTTAAAAGG GGAAAGCTGG CAAGATTTTT
     3541 TACGCAATAA CGCTAAAAGT TTTAGATGTG CTTTACTAAG TCATCGCAAT GGAGCAAAAG
     3601 TACATTCAGA TACACGGCCT ACAGAAAAAC AGTATGAAAC TCTCGAAAAT CAATTAGCCT
     3661 TTTTATGCCA ACAAGGTTTT TCACTAGAGA ACGCGTTATA TGCACTCAGC GCTGTGGGGC
     3721 ATTTTACTTT AGGTTGCGTA TTGGAAGATC AAGAGCATCA AGTCGCTAAA GAAGAAAGGG
     3781 AAACACCTAC TACTGATAGT ATGCCGCCAT TATTACGACA AGCTATCGAA TTATTTGATC
     3841 ACCAAGGTGC AGAGCCAGCC TTCTTATTCG GCCTTGAATT GATCATATGC GGATTAGAAA
     3901 AACAACTTAA ATGTGAAAGT GGGTCCGCGT ACAGCCGCGC GCGTACGAAA AACAATTACG
     3961 GGTCTACCAT CGAGGGCCTG CTCGATCTCC CGGACGACGA CGCCCCCGAA GAGGCGGGGC
     4021 TGGCGGCTCC GCGCCTGTCC TTTCTCCCCG CGGGACACAC GCGCAGACTG TCGACGGCCC
     4081 CCCCGACCGA TGTCAGCCTG GGGGACGAGC TCCACTTAGA CGGCGAGGAC GTGGCGATGG
     4141 CGCATGCCGA CGCGCTAGAC GATTTCGATC TGGACATGTT GGGGGACGGG GATTCCCCGG
     4201 GTCCGGGATT TACCCCCCAC GACTCCGCCC CCTACGGCGC TCTGGATATG GCCGACTTCG
     4261 AGTTTGAGCA GATGTTTACC GATGCCCTTG GAATTGACGA GTACGGTGGG TAGGGCCGAA
     4321 GCCGCTTGGA ATAAGGCCGG TGTGCGTTTG TCTATATGTT ATTTTCCACC ATATTGCCGT
     4381 CTTTTGGCAA TGTGAGGGCC CGGAAACCTG GCCCTGTCTT CTTGACGAGC ATTCCTAGGG
     4441 GTCTTTCCCC TCTCGCCAAA GGAATGCAAG GTCTGTTGAA TGTCGTGAAG GAAGCAGTTC
     4501 CTCTGGAAGC TTCTTGAAGA CAAACAACGT CTGTAGCGAC CCTTTGCAGG CAGCGGAACC
     4561 CCCCACCTGG CGACAGGTGC CTCTGCGGCC AAAAGCCACG TGTATAAGAT ACACCTGCAA
     4621 AGGCGGCACA ACCCCAGTGC CACGTTGTGA GTTGGATAGT TGTGGAAAGA GTCAAATGGC
     4681 TCTCCTCAAG CGTATTCAAC AAGGGGCTGA AGGATGCCCA GAAGGTACCC CATTGTATGG
     4741 GATCTGATCT GGGGCCTCGG TGCACATGCT TTACATGTGT TTAGTCGAGG TTAAAAAAAC
     4801 GTCTAGGCCC CCCGAACCAC GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAATATG
     4861 GCCACAACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA CGACGTCCCC
     4921 AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG CCACACCGTC
     4981 GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT CACGCGCGTC
     5041 GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC GGTCTGGACC
     5101 ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG CATGGCCGAG
     5161 TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC GCCGCACCGG
     5221 CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA CCAGGGCAAG
     5281 GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC
     5341 GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT CGGCTTCACC
     5401 GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC
     5461 GGTGCC
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