ID pEntry-L4-rtTA-IRES-Puro-pA-TRE-R1; SV 1; circular; unknown; STD; UNC; 5466 BP. DT 28-JUL.-2021 DE LMBP 8214. OS unknown CC Accession number: LMBP 8214 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 28-JUL-2021 FH Key Location/Qualifiers FH FT CDS join(4870..5466,1..3) FT /label="puromycin resistance" FT terminator 90..314 FT /label="BGH polyA" FT promoter 374..742 FT /label="hCMVT promoter" FT misc_recomb complement(872..980) FT /label="attR1" FT CDS 1090..1899 FT /label="Tn903 neomycin resistance" FT rep_origin 2359..2761 FT /label="pMB1 ori" FT terminator complement(2840..2883) FT /label="T1 terminator" FT terminator 3015..3042 FT /label="T2 terminator" FT misc_recomb 3116..3211 FT /label="attL4" FT CDS 3309..4313 FT /label="rtTA" FT RBS 4314..4857 FT /label="IRES of EMCV polyprotein gene" SQ SEQUENCE 5466 AA; 1690372 MW; TGACGCCCGC CCCACGACCC GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG 60 ATGATCTAGA GCTCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT 120 TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA 180 ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG 240 GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC 300 GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCTGCAGC CCGGGGGATC CACTAGTTCT 360 AGAGCGGCCG CACTCGAGTT TACCACTCCC TATCAGTGAT AGAGAAAAGT GAAAGTCGAG 420 TTTACCACTC CCTATCAGTG ATAGAGAAAA GTGAAAGTCG AGTTTACCAC TCCCTATCAG 480 TGATAGAGAA AAGTGAAAGT CGAGTTTACC ACTCCCTATC AGTGATAGAG AAAAGTGAAA 540 GTCGAGTTTA CCACTCCCTA TCAGTGATAG AGAAAAGTGA AAGTCGAGTT TACCACTCCC 600 TATCAGTGAT AGAGAAAAGT GAAAGTCGAG TTTACCACTC CCTATCAGTG ATAGAGAAAA 660 GTGAAAGTCG AGCTCGGTAC CCGGGTCGAG GTAGGCGTGT ACGGTGGGAG GCCTATATAA 720 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC 780 TCCATAGAAG ACACCGGGAC CGATCCAGCC TCCGCGGCCC CGAATTCGGT ACCGGATCCA 840 GTCGAGATAT CTAGAAAGGG CGAATTCGAC CCAAGTTTGT ACAAAAAAGT TGAACGAGAA 900 ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA 960 TAATACTGTA AAACACAACA GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA 1020 CAAGATAAAA ATATATCATC ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA 1080 AGGGGTGTTA TGAGCCATAT TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG 1140 GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA 1200 ATCTATCGCT TGTATGGGAA GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT 1260 AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG 1320 CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT 1380 GCGATCCCCG GAAAAACAGC ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT 1440 ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT 1500 CCTTTTAACA GCGATCGCGT ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT 1560 TTGGTTGATG CGAGTGATTT TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG 1620 AAAGAAATGC ATAAACTTTT GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC 1680 TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA 1740 GTCGGAATCG CAGACCGATA CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT 1800 TCTCCTTCAT TACAGAAACG GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT 1860 AAATTGCAGT TTCATTTGAT GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG 1920 TAACATTATT CAGATTGGGC CCCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA 1980 GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA 2040 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA 2100 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC 2160 CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA 2220 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA 2280 CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG 2340 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT 2400 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC 2460 ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC 2520 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC 2580 GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC 2640 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT 2700 ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG 2760 CGCCCAATAC GCAAACCGCT AGCATGGATC TCGGGGACGT CTAACTACTA AGCGAGAGTA 2820 GGGAACTGCC AGGCATCAAA TAAAACGAAA GGCTCAGTCG GAAGACTGGG CCTTTCGTTT 2880 TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGG GAGCGGATTT 2940 GAACGTTGTG AAGCAACGGC CCGGAGGGTG GCGGGCAGGA CGCCCGCCAT AAACTGCCAG 3000 GCATCAAACT AAGCAGAAGG CCATCCTGAC GGATGGCCTT TTTGCGTTTC TACAAACTCT 3060 TCCTGTTAGT TAGTTACTTA AGCTCGGGCC CGCGTTAACG CTACCATGGA GCTCCAAATA 3120 ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATAA GCAATGCTTT 3180 TTTATAATGC CAACTTTGTA TAGAAAAGTT GGCTCCGAAT TCGCCCTTTT AAAGGAACCA 3240 ATTCAGTCGA GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCACCATG CCAAAGAGAC 3300 CCAGACCCTC TAGATTAGAT AAAAGTAAAG TGATTAACAG CGCATTAGAG CTGCTTAATG 3360 AGGTCGGAAT CGAAGGTTTA ACAACCCGTA AACTCGCCCA GAAGCTTGGT GTAGAGCAGC 3420 CTACACTGTA TTGGCATGTA AAAAATAAGC GGGCTTTGCT CGACGCCTTA GCCATTGAGA 3480 TGTTAGATAG GCACCATACT CACTTTTGCC CTTTAAAAGG GGAAAGCTGG CAAGATTTTT 3540 TACGCAATAA CGCTAAAAGT TTTAGATGTG CTTTACTAAG TCATCGCAAT GGAGCAAAAG 3600 TACATTCAGA TACACGGCCT ACAGAAAAAC AGTATGAAAC TCTCGAAAAT CAATTAGCCT 3660 TTTTATGCCA ACAAGGTTTT TCACTAGAGA ACGCGTTATA TGCACTCAGC GCTGTGGGGC 3720 ATTTTACTTT AGGTTGCGTA TTGGAAGATC AAGAGCATCA AGTCGCTAAA GAAGAAAGGG 3780 AAACACCTAC TACTGATAGT ATGCCGCCAT TATTACGACA AGCTATCGAA TTATTTGATC 3840 ACCAAGGTGC AGAGCCAGCC TTCTTATTCG GCCTTGAATT GATCATATGC GGATTAGAAA 3900 AACAACTTAA ATGTGAAAGT GGGTCCGCGT ACAGCCGCGC GCGTACGAAA AACAATTACG 3960 GGTCTACCAT CGAGGGCCTG CTCGATCTCC CGGACGACGA CGCCCCCGAA GAGGCGGGGC 4020 TGGCGGCTCC GCGCCTGTCC TTTCTCCCCG CGGGACACAC GCGCAGACTG TCGACGGCCC 4080 CCCCGACCGA TGTCAGCCTG GGGGACGAGC TCCACTTAGA CGGCGAGGAC GTGGCGATGG 4140 CGCATGCCGA CGCGCTAGAC GATTTCGATC TGGACATGTT GGGGGACGGG GATTCCCCGG 4200 GTCCGGGATT TACCCCCCAC GACTCCGCCC CCTACGGCGC TCTGGATATG GCCGACTTCG 4260 AGTTTGAGCA GATGTTTACC GATGCCCTTG GAATTGACGA GTACGGTGGG TAGGGCCGAA 4320 GCCGCTTGGA ATAAGGCCGG TGTGCGTTTG TCTATATGTT ATTTTCCACC ATATTGCCGT 4380 CTTTTGGCAA TGTGAGGGCC CGGAAACCTG GCCCTGTCTT CTTGACGAGC ATTCCTAGGG 4440 GTCTTTCCCC TCTCGCCAAA GGAATGCAAG GTCTGTTGAA TGTCGTGAAG GAAGCAGTTC 4500 CTCTGGAAGC TTCTTGAAGA CAAACAACGT CTGTAGCGAC CCTTTGCAGG CAGCGGAACC 4560 CCCCACCTGG CGACAGGTGC CTCTGCGGCC AAAAGCCACG TGTATAAGAT ACACCTGCAA 4620 AGGCGGCACA ACCCCAGTGC CACGTTGTGA GTTGGATAGT TGTGGAAAGA GTCAAATGGC 4680 TCTCCTCAAG CGTATTCAAC AAGGGGCTGA AGGATGCCCA GAAGGTACCC CATTGTATGG 4740 GATCTGATCT GGGGCCTCGG TGCACATGCT TTACATGTGT TTAGTCGAGG TTAAAAAAAC 4800 GTCTAGGCCC CCCGAACCAC GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAATATG 4860 GCCACAACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA CGACGTCCCC 4920 AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG CCACACCGTC 4980 GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT CACGCGCGTC 5040 GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC GGTCTGGACC 5100 ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG CATGGCCGAG 5160 TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC GCCGCACCGG 5220 CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA CCAGGGCAAG 5280 GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC 5340 GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT CGGCTTCACC 5400 GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC 5460 GGTGCC 5466 //